scholarly journals GENE EXPRESSION ANALYSES OF SOME TRANSPOSONS, PHYSIOLOGICAL AND MORPHOLOGICAL CHANGES IN MELON CULTIVARS EXPOSED TO DROUGHT STRESS

2022 ◽  
Vol 32 (4) ◽  
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Morphological Changes ◽  
Gene Expression Analyses

scholarly journals Shaping Durum Wheat for the Future: Gene Expression Analyses and Metabolites Profiling Support the Contribution of BCAT Genes to Drought Stress Response

2020 ◽  
Vol 11 ◽  
Author(s):  
Valentina Buffagni ◽  
Filippo Vurro ◽  
Michela Janni ◽  
Mariolina Gullì ◽  
Arturo A. Keller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Stress Response ◽  
Durum Wheat ◽  
Gene Expression Analyses ◽  
The Future

Morphologic and anatomic response, catalase gene expression in drought varieties of tomato and bioinformatics analyses of microarray studies of the catalase gene

2021 ◽  
Author(s):  
Hanieh Mohajjel Shoja ◽  
Taha Khezriani ◽  
Maryam Kolahi ◽  
Elham Elham Mohajel Kazemi ◽  
Milad Yazdi
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Real Time ◽  
Morphological Changes ◽  
Field Capacity ◽  
Bioinformatics Analyses ◽  
Catalase Gene ◽  
Significant Difference ◽  
Drought Conditions ◽  
Microarray Studies

Abstract Crops in arid and semi-arid regions are exposed to adverse environmental factors such as drought. Experiments were conducted to determine the morphologic and anatomic response of drought-susceptible and tolerant varieties of tomato (Solanum lycopersicum L.) under drought conditions (100%, 75%, 50%, 25% of field capacity). To investigate the role of antioxidant enzyme, catalase gene expression was examined by real-time RT-qPCR and microarray studies of the catalase gene in tomatoes under stress examined utilizing bioinformatics. The results showed significant morphological changes under drought conditions. Anatomical studies revealed that CaljN3 is more resistant than SuperstrainB varieties under drought stress. Relative expression of the CAT1 gene did not show any significant difference in both Caljn3 and SuperstrainB varieties based on quantitative Real-Time PCR, under drought stress. The bioinformatics results from microarray analysis revealed that this gene did not show a significant difference in expression in any of the cultivars and under any of the stresses. This gene is in the conserve cluster, a cluster with 118 members and a z score of 14.26148. This showed that this cluster is fully protected between two susceptible and tolerant varieties. The enrichment gene of this cluster did not show any significant intracellular pathways. It appears that in response to stress, an activating mechanism other than catalase is necessary. The fight against oxidative stress may begin one step before that of the enzymes and seeks to combat the stressor by activating proteins, especially channels, pumps and some cellular messengers.

Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage)

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Made Pharmawati ◽  
Ni Nyoman Wirasiti ◽  
Luh Putu Wrasiati
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Control Plant ◽  
Reproductive Stage ◽  
Limiting Factors ◽  
Rice Grain ◽  
Aquaporin Gene ◽  
Peg Treatment

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice

scholarly journals Unearthing LTR retrotransposon gag genes co-opted in the deep evolution of eukaryotes

2021 ◽  
Author(s):  
Jianhua Wang ◽  
Guan-Zhu Han
Keyword(s):  
Gene Expression ◽  
Selective Pressure ◽  
Ltr Retrotransposon ◽  
Ltr Retrotransposons ◽  
Cellular Functions ◽  
The Family ◽  
Family Level ◽  
Gene Expression Analyses ◽  
Comprehensive Picture ◽  
Gypsy Retrotransposons

Abstract LTR retrotransposons comprise a major component of the genomes of eukaryotes. On occasion, retrotransposon genes can be recruited by their hosts for diverse functions, a process formally referred to as co-option. However, a comprehensive picture of LTR retrotransposon gag gene co-option in eukaryotes is still lacking, with several documented cases exclusively involving Ty3/Gypsy retrotransposons in animals. Here we use a phylogenomic approach to systemically unearth co-option of retrotransposon gag genes above the family level of taxonomy in 2,011 eukaryotes, namely co-option occurring during the deep evolution of eukaryotes. We identify a total of 14 independent gag gene co-option events across more than 740 eukaryote families, eight of which have not been reported previously. Among these retrotransposon gag gene co-option events, nine, four, and one involve gag genes of Ty3/Gypsy, Ty1/Copia, and Bel-Pao retrotransposons, respectively. Seven, four, and three co-option events occurred in animals, plants, and fungi, respectively. Interestingly, two co-option events took place in the early evolution of angiosperms. Both selective pressure and gene expression analyses further support that these co-opted gag genes might perform diverse cellular functions in their hosts, and several co-opted gag genes might be subject to positive selection. Taken together, our results provide a comprehensive picture of LTR retrotransposon gag gene co-option events that occurred during the deep evolution of eukaryotes, and suggest paucity of LTR retrotransposon gag gene co-option during the deep evolution of eukaryotes.

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