Characterization of Interaction between Wheat Roots with Different Resistance and Heterodera filipjevi

2013 ◽  
Vol 38 (6) ◽  
pp. 1009-1017 ◽  
Author(s):  
Lei CUI ◽  
Xiu GAO ◽  
Xiao-Ming WANG ◽  
Heng JIAN ◽  
Wen-Hua TANG ◽  
...  
Keyword(s):  
Wheat Roots ◽  
Heterodera Filipjevi

scholarly journals Characterization of Dynamic Regulatory Gene and Protein Networks in Wheat Roots Upon Perceiving Water Deficit Through Comparative Transcriptomics Survey

2021 ◽  
Vol 12 ◽  
Author(s):  
Yousef Rahimi ◽  
Pär K. Ingvarsson ◽  
Mohammad Reza Bihamta ◽  
Hadi Alipour ◽  
Alireza Taleei ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Water Deficit ◽  
Regulatory Gene ◽  
Field Trials ◽  
Glutathione Metabolism ◽  
Cell Periphery ◽  
Wheat Roots ◽  
Ppi Networks ◽  
Extracellular Region

A well-developed root system benefits host plants by optimizing water absorption and nutrient uptake and thereby increases plant productivity. In this study we have characterized the root transcriptome using RNA-seq and subsequential functional analysis in a set of drought tolerant and susceptible genotypes. The goal of the study was to elucidate and characterize water deficit-responsive genes in wheat landraces that had been through long-term field and biochemical screening for drought tolerance. The results confirm genotype differences in water-deficit tolerance in line with earlier results from field trials. The transcriptomics survey highlighted a total of 14,187 differentially expressed genes (DEGs) that responded to water deficit. The characterization of these genes shows that all chromosomes contribute to water-deficit tolerance, but to different degrees, and the B genome showed higher involvement than the A and D genomes. The DEGs were mainly mapped to flavonoid, phenylpropanoid, and diterpenoid biosynthesis pathways, as well as glutathione metabolism and hormone signaling. Furthermore, extracellular region, apoplast, cell periphery, and external encapsulating structure were the main water deficit-responsive cellular components in roots. A total of 1,377 DEGs were also predicted to function as transcription factors (TFs) from different families regulating downstream cascades. TFs from the AP2/ERF-ERF, MYB-related, B3, WRKY, Tify, and NAC families were the main genotype-specific regulatory factors. To further characterize the dynamic biosynthetic pathways, protein-protein interaction (PPI) networks were constructed using significant KEGG proteins and putative TFs. In PPIs, enzymes from the CYP450, TaABA8OH2, PAL, and GST families play important roles in water-deficit tolerance in connection with MYB13-1, MADS-box, and NAC transcription factors.

scholarly journals Protein and lipid characterization of wheat roots plasma membrane damaged by Fe and H<sub>2</sub>O<sub>2</sub> using ATR-FTIR method

2013 ◽  
Vol 04 (01) ◽  
pp. 28-35 ◽  
Author(s):  
Xin Zhao ◽  
Xiaoju Yang ◽  
Yong Shi ◽  
Guoxiong Chen ◽  
Xinrong Li
Keyword(s):  
Plasma Membrane ◽  
Wheat Roots ◽  
Lipid Characterization

scholarly journals Network Analysis of Plasmidomes: The Azospirillum brasilense Sp245 Case

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Valerio Orlandini ◽  
Giovanni Emiliani ◽  
Marco Fondi ◽  
Isabel Maida ◽  
Elena Perrin ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Molecular Mechanisms ◽  
Gene Loss ◽  
Functional Characterization ◽  
Evolutionary Relationships ◽  
Computational Pipeline ◽  
Wheat Roots ◽  
Source Organism ◽  
Evolutionary Pathways

Azospirillum brasilense is a nitrogen-fixing bacterium living in association with plant roots. The genome of the strain Sp245, isolated in Brazil from wheat roots, consists of one chromosome and six plasmids. In this work, the A. brasilense Sp245 plasmids were analyzed in order to shed some light on the evolutionary pathways they followed over time. To this purpose, a similarity network approach was applied in order to identify the evolutionary relationships among all the A. brasilense plasmids encoded proteins; in this context a computational pipeline specifically devoted to the analysis and the visualization of the network-like evolutionary relationships among different plasmids molecules was developed. This information was supplemented with a detailed (in silico) functional characterization of both the connected (i.e., sharing homology with other sequences in the dataset) and the unconnected (i.e., not sharing homology) components of the network. Furthermore, the most likely source organism for each of the genes encoded by A. brasilense plasmids was checked, allowing the identification of possible trends of gene loss/gain in this microorganism. Data obtained provided a detailed description of the evolutionary landscape of the plasmids of A. brasilense Sp245, suggesting some of the molecular mechanisms responsible for the present-day structure of these molecules.

scholarly journals Sensitive and Direct Detection of Heterodera filipjevi in Soil and Wheat Roots by Species-Specific SCAR-PCR Assays

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1288-1294 ◽  
Author(s):  
Huan Peng ◽  
Xiaoli Qi ◽  
Deliang Peng ◽  
Haibo Long ◽  
Xufeng He ◽  
...  
Keyword(s):  
Direct Detection ◽  
Parasitic Nematodes ◽  
Cyst Nematodes ◽  
Specific Sequence ◽  
Wheat Roots ◽  
Sequence Characterized Amplified Region ◽  
Field Samples ◽  
Pcr Assays ◽  
Heterodera Filipjevi ◽  
Species Specific

Cereal cyst nematodes are the most important plant-parasitic nematodes on cereal crops in wheat producing areas of the world. Heterodera filipjevi was first reported in China in 2010. In this study, species-specific sequence characterized amplified region–polymerase chain reaction (SCAR-PCR) assays for detection and identification of H. filipjevi from infected wheat roots and soil were developed. The species-specific primers were designed according to the randomly amplified polymorphic DNA (RAPD) markers amplified with random primer OPK16. A 646-bp specific fragment of sequence was generated, which characterized amplified regions in H. filipjevi. The detection limitation of the PCR assay was as low as 0.125 μl second-stage juvenile (J2) lysate, 3.9 × 10–3 μl adult female lysate, and 10–3 μl cyst lysate. The method was able to detect the various stages (J2, J3, J4, and female) of H. filipjevi, and a single of nematode in 0.5 g of soil. H. filipjevi was detected by the method in two of six field samples, and one of those samples contained a mixed population of H. filipjevi and H. avenae. This study is the first to provide a definitive diagnostic assay for H. filipjevi in wheat roots and soil.

scholarly journals A Study of Wheat Roots Infected with Ophiobolus Graminis (Sacc.) Using Fluorescence and Electron Microscopy

1971 ◽  
Vol 24 (3) ◽  
pp. 815 ◽  
Author(s):  
AA Holland ◽  
RG Fulcher
Keyword(s):  
Electron Microscopy ◽  
Tobacco Mosaic Virus ◽  
New South ◽  
South Australia ◽  
Wheat Roots ◽  
Isolation And Characterization ◽  
South Wales ◽  
Fluorescence And Electron Microscopy ◽  
Unusual Strain

Virus-like symptoms were observed on leaves of frangipani (Plurneria acutifolia Poir.) from several gardens in South Australia and New South Wales. The leaves showed chlorotic ringspots or mosaic and were often distorted (Fig. 1). As far as we are aware no viruses from frangipani have been previously isolated. This communica-tion is concerned with the isolation and characterization of an unusual strain of tobacco mosaic virus (TMV) from frangipani.

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