Design of fluorescence immune-chromatographic quantitative detection system

Abstract Background: The aim of this study was to compare the ABI PRISM 7700 Sequence Detection System and the LightCycler to develop a quantitative real-time PCR assay for the detection of human cytomegalovirus (HCMV) DNA suitable for routine hospital application. Methods: We used one exonuclease probe and five different hybridization probe sets as sequence-specific fluorescence detection formats. For the exonuclease assay and two hybridization probe sets, reproducibility and the detection limit were determined. To keep the total assay time to a minimum, we gradually shortened individual reaction steps on both instruments. Results: The exonuclease assay can be interchangeably performed on the 7700 and the LightCycler. No change of reaction conditions is required, except for the addition of bovine serum albumin to the LightCycler reaction. The shortest possible total assay time is 80 min for the ABI PRISM 7700 Sequence Detection System and 20 min for the LightCycler. When the LightCycler is used, the exonuclease probe can be replaced by a set of hybridization probes. All assays presented here detected HCMV DNA in a linear range from 101 to 107 HCMV genome equivalents/assay (r >0.995) with low intraassay (<5%) and interassay (<10%) variation. Conclusions: The ABI PRISM 7700 Sequence Detection System as well as the LightCycler are useful instruments for rapid and precise online PCR detection. Moreover, the two principles of fluorescence signal production allow HCMV quantification with the same accuracy.

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Ultraviolet Spectral Analysis and Quantitative Detection of Heptafluoroisobutyronitrile (C4F7N) in a C4F7N–Carbon Dioxide (CO2) Gas Mixture

Applied Spectroscopy ◽

10.1177/0003702819833702 ◽

2019 ◽

Vol 73 (8) ◽

pp. 917-926 ◽

Cited By ~ 3

Author(s):

Yin Zhang ◽

Xiaoxing Zhang ◽

Chang Liu ◽

Yi Li ◽

Zhaolun Cui ◽

...

Keyword(s):

Carbon Dioxide ◽

Absorption Spectroscopy ◽

Density Functional ◽

Sulfur Hexafluoride ◽

Detection System ◽

Spectral Characteristics ◽

Uv Absorption ◽

Quantitative Detection ◽

Gas Mixture ◽

Uv Absorption Spectroscopy

As an alternative to sulfur hexafluoride (SF6) with great potential for application, heptafluoroisobutyronitrile–carbon dioxide (C4F7N–CO2) gas mixture has been applied in various gas-insulated equipment. The insulation performance of the gas mixture is closely related to the mixing ratio. Therefore, accurate quantification of C4F7N in the C4F7N–CO2 gas mixture has very important engineering significance. At present, there are few reports on the rapid quantitative detection of the concentration of C4F7N in the gas mixture. In this paper, a rapid analytical method for C4F7N concentration based on ultraviolet (UV) absorption spectroscopy is constructed. The UV spectral characteristics of C4F7N molecules are calculated by density functional theory. The appropriate bands that can be detected are determined by analyzing the calculated results. A concentration detection system of C4F7N based on UV absorption spectroscopy is built. Through analysis of the calculated results and experimental results, a quantitative detection method of C4F7N in the C4F7N/CO2 gas mixture is determined. The method can achieve accurate detection of the concentration of the gas mixture in the conventional application range (including 4–10% C4F7N). The coefficient of the determination R2 of the concentration inversion curve reaches 0.999 and the inversion error ratio does not exceed 5%. The related research results provide an important reference for the engineering application of the gas mixture.

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Analytical characterization of IgG–cTpp and IgG–Mn-cTpp conjugates

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424615500984 ◽

2015 ◽

Vol 19 (11) ◽

pp. 1177-1184

Author(s):

Kamila Konopińska ◽

Mariusz Pietrzak ◽

Radosław Mazur ◽

Elżbieta Malinowska

Keyword(s):

Detection System ◽

Size Exclusion ◽

Quantitative Detection ◽

Measured Signal ◽

Indirect Determination ◽

Exclusion Chromatography ◽

Model Protein ◽

Spectrofluorimetric Detection ◽

Secondary Antibodies

Herein, the conjugation of carboxylated tetraphenylporphyrin or its derivative containing manganese cation and model protein — immunoglobulin G is presented. The obtained IgG–cTpp and IgG–Mn-cTpp conjugates were subsequently used for model immunoassays construction. The IgG–cTpp formation was confirmed using size-exclusion chromatography. Thanks to the unique properties of applied labels the assay analysis was carried out with both spectrophotometric and spectrofluorimetric detection. The assays were performed creating semi-quantitative detection system using 96-well plates. The incubation time, ensuring full saturation of the surface with secondary antibodies was also optimized. Moreover, in the case of IgG–Mn-cTpp conjugates we present the possibility of both direct and indirect determination of the label, the latter based on the catalytic activity of Mn-cTpp, which allows for amplification of the measured signal. We proved that both cTpp and Mn-cTpp may be successfully used for protein labeling and serve as universal tracers for various formats of affinity assays and sensors.

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Broken Steel Wire Rope Inspection Based on RBF Neural Network

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.614-615.1734 ◽

2012 ◽

Vol 614-615 ◽

pp. 1734-1737 ◽

Cited By ~ 1

Author(s):

Zhen Chen ◽

Yan Mei Liu

Keyword(s):

Neural Network ◽

Detection System ◽

Rbf Neural Network ◽

Steel Wire ◽

Wire Rope ◽

Experimental Results ◽

Quantitative Detection ◽

Design Scheme ◽

Steel Rope ◽

Steel Wires

In view of the problem of low detection precision exists in traditional quantitative detection system of broken wire for steel rope, the paper proposed a design scheme of quantitative detection system of broken wire for steel rope based on RBF neural network. The RBF Neural Network was introduced to establish the model of discerning quantitative broken steel wires. Experimental results showed that the method performed well in the identification of damage.

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Quantitative Detection of Trichloroethylene-Degrading Mycobacterium sp. TA27 with a Real-time PCR Product Detection System.

Microbes and Environments ◽

10.1264/jsme2.2001.109 ◽

2001 ◽

Vol 16 (2) ◽

pp. 109-116 ◽

Cited By ~ 1

Author(s):

Akiko Hashimoto ◽

Kazuhiro Iwasaki ◽

Mutsuyasu Nakajima ◽

Osami Yagi

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Detection System ◽

Quantitative Detection ◽

Pcr Product

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Quantitative detection system for immunostrips in 180nm standard CMOS technology

Analog Integrated Circuits and Signal Processing ◽

10.1007/s10470-020-01712-y ◽

2020 ◽

Author(s):

Engincan Tekin ◽

Caner Celikdemir ◽

Busra Ucar ◽

Ozgur Gul ◽

Baykal Sarioglu

Keyword(s):

Detection System ◽

Cmos Technology ◽

Quantitative Detection

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Quantitative Detection of C2H2 Gas Based on an Infrared Laser Gas Sensor

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1092-1093.400 ◽

2015 ◽

Vol 1092-1093 ◽

pp. 400-406

Author(s):

Jia Gui Tao ◽

Jing Lei Shi ◽

Xiao Xing Zhang

Keyword(s):

Gas Sensor ◽

Volume Fraction ◽

Detection System ◽

Least Square Method ◽

Infrared Laser ◽

State Assessment ◽

Least Square ◽

Transformer Oil ◽

System Structure ◽

Quantitative Detection

Detection of C2H2 gas in transformer oil is significant for diagnosing the operating state assessment of power equipment. The paper develops an infrared laser gas sensor to detect the C2H2 gas in transformer oil, and also introduces the system structure in detail. A gas-absorbed laser cell that contains a series of laser reflectors is designed and used in our detection system, which adds the optical path without changing the volume of the cell. 1529.16nm is chosen as the characteristic spectrum line of C2H2 gas, and concentration of C2H2 gas is quantitatively analyzed based on least square method. The experiment result shows that in certain volume fraction range of acetylene, the absorbance has a good linear relationship with the acetylene concentration, and the minimum detection limit for acetylene is 10μL/L. In general, the developed infrared laser gas sensor can detect C2H2 gas in transformer oil effectively.

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