scholarly journals Peptide-modified Chitosan Hydrogel accelerates skin wound healing by promoting fibroblast proliferation, migration and secretion

2017 ◽  
Author(s):  
Xionglin Chen ◽  
Min Zhang ◽  
Shixuan Chen ◽  
Xueer Wang ◽  
Zhihui Tian ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Fibroblast Proliferation ◽  
Skin Wound Healing ◽  
Chitosan Hydrogel ◽  
Modified Chitosan

scholarly journals SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2611 ◽  
Author(s):  
Xionglin Chen ◽  
Xiaoming Cao ◽  
He Jiang ◽  
Xiangxin Che ◽  
Xiaoyuan Xu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Skin Wound ◽  
Matrix Remodeling ◽  
Skin Wounds ◽  
Skin Substitutes ◽  
Skin Wound Healing ◽  
Chitosan Hydrogel ◽  
Modified Chitosan ◽  
Positive Controls

: Skin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment strategies that enhance angiogenesis and growth factors secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H&E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that seen in the negative and positive controls. Masson’s trichrome staining showed that more collagen fibers were deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. Immunohistochemistry assays demonstrated that more myofibroblasts were deposited and more angiogenesis occurred in skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. In addition, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of growth factors in skin wounds. Taken together, these results suggest that the SIKVAV-modified chitosan hydrogel has the potential to be developed as synthesized biomaterials for the treatment of skin wounds.

scholarly journals Peptide-Modified Chitosan Hydrogels Accelerate Skin Wound Healing by Promoting Fibroblast Proliferation, Migration, and Secretion

2017 ◽  
Vol 26 (8) ◽  
pp. 1331-1340 ◽  
Author(s):  
Xionglin Chen ◽  
Min Zhang ◽  
Shixuan Chen ◽  
Xueer Wang ◽  
Zhihui Tian ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Skin Wound ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Promote Cell Proliferation ◽  
Modified Chitosan ◽  
Chitosan Hydrogels ◽  
Positive Controls

Skin wound healing is a complicated process that involves a variety of cells and cytokines. Fibroblasts play an important role in this process and participate in transformation into myofibroblasts, the synthesis of extracellular matrix (ECM) and fibers, and the secretion of a variety of growth factors. This study assessed the effects of peptide Ser-Ile-Lys-Val-Ala-Val (SIKVAV)--modified chitosan hydrogels on skin wound healing. We investigated the capability of peptide SIKVAV to promote cell proliferation and migration, the synthesis of collagen, and the secretion of a variety of growth factors using fibroblasts in vitro. We also treated skin wounds established in mice using peptide SIKVAV-modified chitosan hydrogels. Hematoxylin and eosin staining showed that peptide-modified chitosan hydrogels enhanced the reepithelialization of wounds compared with negative and positive controls. Masson’s trichrome staining demonstrated that more collagen fibers were deposited in the wounds treated with peptide-modified chitosan hydrogels compared with the negative and positive controls. Immunohistochemistry revealed that the peptide-modified chitosan hydrogels promoted angiogenesis in the skin wound. Taken together, these results suggest that peptide SIKVAV-modified chitosan hydrogels may be useful in wound dressing and the treatment of skin wounds.

A laminin mimetic peptide SIKVAV-conjugated chitosan hydrogel promoting wound healing by enhancing angiogenesis, re-epithelialization and collagen deposition

2015 ◽  
Vol 3 (33) ◽  
pp. 6798-6804 ◽  
Author(s):  
Shixuan Chen ◽  
Min Zhang ◽  
Xuebing Shao ◽  
Xueer Wang ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Collagen Deposition ◽  
Critical Factors ◽  
Skin Wound Healing ◽  
Chitosan Hydrogel ◽  
Mimetic Peptide

Angiogenesis and re-epithelialization are critical factors in skin wound healing.

scholarly journals Improvement of skin wound healing in diabetic mice by kinin B2 receptor blockade

2015 ◽  
Vol 130 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Dorinne Desposito ◽  
Catherine Chollet ◽  
Christopher Taveau ◽  
Vincent Descamps ◽  
François Alhenc-Gelas ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Models ◽  
Receptor Activation ◽  
Skin Wound ◽  
Receptor Blockade ◽  
Fibroblast Proliferation ◽  
Diabetic Mice ◽  
Skin Wound Healing ◽  
Normal Wound Healing

Kinin B2 receptor activation impairs skin wound healing in mice, in part through imbalance of keratinocyte/fibroblast proliferation. Such activation occurs in diabetes and contributes to delaying wound healing. B2 receptor blockade restores the normal wound healing pattern in mouse models of diabetes.

scholarly journals The Nf1 Tumor Suppressor Regulates Mouse Skin Wound Healing, Fibroblast Proliferation, and Collagen Deposited by Fibroblasts

1999 ◽  
Vol 112 (6) ◽  
pp. 835-842 ◽  
Author(s):  
Radhika P. Atit ◽  
Maria J. Crowe ◽  
David G. Greenhalgh ◽  
Richard J. Wenstrup ◽  
Nancy Ratner
Keyword(s):  
Wound Healing ◽  
Tumor Suppressor ◽  
Mouse Skin ◽  
Skin Wound ◽  
Fibroblast Proliferation ◽  
Skin Wound Healing

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

scholarly journals The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 457
Author(s):  
Andreu Blanquer ◽  
Jana Musilkova ◽  
Elena Filova ◽  
Johanka Taborska ◽  
Eduard Brynda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Chronic Wounds ◽  
Human Keratinocytes ◽  
Skin Wound ◽  
Platelet Lysate ◽  
Therapeutic Approaches ◽  
Skin Wound Healing ◽  
New Therapeutic Approaches ◽  
Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

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