Trilobatin Protects Cardiomyocytes from Hypoxia/ Reperfusion Injury by Regulating the Nuclear Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway

Trilobatin is a natural plant-derived glycosylated flavonoid that has been shown to exhibit multiple beneficial pharmacologic activities including protection of heart against H/R-induced cardiomyocyte injury. However, the molecular mechanisms underlying protection from H/R-induced cardiomyocyte injury remain unknown. Using H9C2 cells as a model, we examined the effect of trilobatin on H/R-induced cellular injury, apoptosis, and generation of reactive oxygen species. The results showed that trilobatin protected H9C2 cells not only from cell death and apoptosis, but also counteracted H/R-induced changes in malondialdehyde, superoxide dismutase, glutathione, and glutathione peroxidase. The evaluation of the mechanism underlying the effect of trilobatin on protection from H/R-induced cellular injury suggested changes in the regulation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 pathway.

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Sodium arsenite induced reactive oxygen species generation, nuclear factor (erythroid-2 related) factor 2 activation, heme oxygenase-1 expression, and glutathione elevation in Chang human hepatocytes

Environmental Toxicology ◽

10.1002/tox.20731 ◽

2011 ◽

Vol 28 (7) ◽

pp. 401-410 ◽

Cited By ~ 29

Author(s):

Bing Li ◽

Xin Li ◽

Bo Zhu ◽

Xinyu Zhang ◽

Yi Wang ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Heme Oxygenase ◽

Sodium Arsenite ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Human Hepatocytes ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Nuclear Factor ◽

Oxygen Species

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Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/9580234 ◽

2016 ◽

Vol 2016 ◽

pp. 1-14 ◽

Cited By ~ 6

Author(s):

Eun Sik Choi ◽

Yun Jung Lee ◽

Chang Seob Seo ◽

Jung Joo Yoon ◽

Byung Hyuk Han ◽

...

Keyword(s):

Vascular Endothelium ◽

Molecular Mechanisms ◽

Water Extract ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Nuclear Factor ◽

Ros Production ◽

Herbal Formula ◽

Protective Effects ◽

Monocyte Adhesion

Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-κB) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-κB and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-κB was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-κB translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-αin HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.

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6-Gingerol Ameliorates Sepsis Induced Acute Lung Injury by Regulating Nuclear Factor-κB and Nuclear-Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Signaling Pathways

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.19:255-260 ◽

2020 ◽

Vol 19 (3) ◽

pp. 255-260

Author(s):

Fan Yang ◽

Lu Deng ◽

MuHu Chen ◽

Ying Liu ◽

Jianpeng Zheng

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Heme Oxygenase ◽

Interleukin 1 ◽

Nuclear Factor Κb ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Nuclear Factor ◽

Alveolar Collapse ◽

Factor Α

Acute lung injury initiated systemic inflammation leads to sepsis. Septic mice show a series of degenerative changes in lungs as demonstrated by pulmonary congestion, alveolar collapse, inflammatory cell infiltration, and increased wet-todry weight in lungs. 6-Gingerol ameliorates histopathological changes and clinical outcome of the sepsis. The increase in the levels of tumor necrosis factor-α, interleukin-1 beta, interleukin-6, and interleukin-18 in septic mice were reduced by administration with 6-Gingerol. Also, 6-Gingerol attenuates sepsis-induced increase of malonaldehyde and decrease of catalase, superoxide, and glutathione. Enhanced phospho-p65, reduced nuclear factor erythropoietin-2-related factor 2, and heme oxygenase 1 in septic mice were reversed by administration with 6-Gingerol. In conclusion, 6-Gingerol demonstrates anti-inflammatory and antioxidant effects against sepsis associated acute lung injury through inactivation of nuclear factor-kappa B and activation of nuclear-factor erythroid 2-related factor 2 pathways.

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