The Investigation of the Neopluramycin � DNA System by UV-visible Spectroscopy

2008 ◽  
Vol 59 (9) ◽  
Author(s):  
Loredana Elena V�jan
Keyword(s):  
Absorption Coefficient ◽  
Binding Constant ◽  
The Self ◽  
Molar Absorption Coefficient ◽  
Water Mixture ◽  
Visible Spectroscopy ◽  
Dimerization Constant ◽  
Calf Thymus ◽  
Uv Visible ◽  
Self Association

The self-association of neopluramycin and the binding of this drug to calf thymus DNA were investigated by using UV-visible spectroscopy. This classical pluramycin antibiotic self-associates in 1:1 ethanol - water mixture. Starting from a simple dimerization model, the molar absorption coefficient of monomer, the molar absorption coefficient of dimer and the dimerization constant were determined. The binding constant of neopluramycin to DNA was determined using Wolfe and Scatchard methods.

Characterization of the Interaction of Amphotericin B with Cholesteryl Trifluoromethylphenyl-Carbamate by UV-Visible Spectroscopy

2008 ◽  
Vol 59 (3) ◽  
pp. 297-299
Author(s):  
Loredana Elena Vijan ◽  
Carmen Mihaela Topala
Keyword(s):  
Absorption Coefficient ◽  
Amphotericin B ◽  
Molar Absorption Coefficient ◽  
Polyene Antibiotic ◽  
Visible Spectroscopy ◽  
Dimerization Constant ◽  
Cooperativity Effects ◽  
Uv Visible ◽  
Self Association

Amphotericin B, a polyene antibiotic, self-associates in ethanol. The self-association of antibiotic has characterized by UV-visible spectroscopy. Starting from a simple dimerization model and using different methods described in literature, one determined the molar absorption coefficient of monomer, the molar absorption coefficient of dimer and the dimerization constant. The binding of amphotericin B to cholesteryl trifluoromethylphenyl-carbamate has investigated using absorbance measurements and the results have rationalized in terms of literature models, taking into account both 1:1 drug-sterol system and cooperativity effects. The binding constant of amphotericin B to cholesteryl trifluoromethylphenyl-carbamate has determined using Benesi-Hildebrand, Scott and Scatchard methods.

FT-near-IR and FT-IR Studies of Cis-9-Octadecen-1-ol: Molar Absorption Coefficient of a Band Due to the First Overtone of the Stretching Mode of the Terminal OH Group and Comparison of Anharmonicity of an OH Stretching Mode among the Monomeric and Associated Species

1995 ◽  
Vol 49 (11) ◽  
pp. 1661-1668 ◽  
Author(s):  
Yongliang Liu ◽  
Hisashi Maeda ◽  
Yukihiro Ozaki ◽  
Mirosław A. Czarnecki ◽  
Masao Suzuki ◽  
...  
Keyword(s):  
Absorption Coefficient ◽  
Near Infrared ◽  
Molar Absorption Coefficient ◽  
Open Chain ◽  
Oh Groups ◽  
Ir Studies ◽  
Near Ir ◽  
Ft Ir ◽  
Self Association ◽  
Associated Species

Fourier transform near-infrared (FT-near-IR) and FT-IR spectra have been measured for cis-9-octadecen-1-ol in CCl4 solutions at various concentrations and temperatures partly to investigate its self-association and partly to explore its near-IR spectrum. Both spectra have demonstrated the appearances of bands due to free terminal OH groups of the linear dimer (open-chain dimer) and oligomer of the alcohol. They are observed at 3621 and 7070 cm−1 in the IR and near-IR regions, whose frequencies are lower by 16 and 34 cm−1, respectively, than those of the corresponding bands due to a free OH group of the monomer. The spectral changes in both regions suggest that (1) at concentrations below 0.10 M, the alcohol exists mostly as the monomer and dimer, and with increasing temperature the dimer dissociates into the monomer; and (2) as the concentration is increased, it associates to form aggregates (such as tetramer), and with temperature increase the aggregates dissociate into the dimer and monomer. The molar absorption coefficient of the band due to the first overtone of the stretching mode of the terminal OH groups has been determined to be 0.139 dm3 mol−1 cm−1. It is approximately 8% of that of the first overtone of the monomeric OH group (1.63 dm3 mol−1 cm−1). The anharmonicity of an OH stretching mode of the alcohol has been investigated for various OH groups, free, terminal, and hydrogen-bonded, by comparing the frequencies of the bands due to the OH stretching modes with those of the bands due to the corresponding first overtones. The anharmonicity of the stretching mode of the terminal OH groups of the associated species is very similar to that for the free OH group of the monomer.

scholarly journals 5-Ureido-3,3-diphenyl-3H-naphtho[2,1-b]pyrans: Photoswitchable self-assembling architectures

2004 ◽  
Vol 6 (4) ◽  
pp. 169-173 ◽  
Author(s):  
X. Sallenave ◽  
S. Delbaere ◽  
S. Ahmed ◽  
G. Vermeersch ◽  
J. L. Pozzo
Keyword(s):  
Carbonyl Group ◽  
Uv Irradiation ◽  
2D Nmr ◽  
Intramolecular Interactions ◽  
The Self ◽  
Visible Spectroscopy ◽  
Photochromic Properties ◽  
Self Assembling ◽  
Amino Derivatives ◽  
Uv Visible

5-Ureido-3,3-diphenyl-3H-naphtho[2,1-b]pyrans have been synthesized and their photochromic properties have been quantified using UV-Visible spectroscopy. The urea pattern is responsive for the selfassembling properties under both colourless and coloured forms whereas the corresponding amino derivatives do not show any aggregative properties. The geometries of the different states have been assigned using1Hand19FNMR spectroscopies, and thereafter their respective kind of aggregates could be proposed through the different inter- and intramolecular interactions. Upon UV irradiation, the pyranic ring is opened and the resulting photogenerated carbonyl group is competing with the urea in the self-assembling process and consequently strongly affects the supramolecular assemblies. The inter- and intramolecular contributions have been quantified through 1D and 2D NMR experiments along with the variation in the concentration.

Nucleic Acid (Calf Thymus-DNA, Yeast tRNA) Binding and Cytotoxic Properties of a Dinuclear (Ru,Co) Metal Polypyridyl Complex

2010 ◽  
Vol 63 (10) ◽  
pp. 1453 ◽  
Author(s):  
Xi-Ling Liang ◽  
Li-Feng Tan
Keyword(s):  
Nucleic Acid ◽  
Equilibrium Dialysis ◽  
Calf Thymus Dna ◽  
Visible Spectroscopy ◽  
Yeast Trna ◽  
Polypyridyl Complex ◽  
Calf Thymus ◽  
Tumour Cell Lines ◽  
Uv Visible ◽  
Trna Binding

Based on [L2Ru{DPPZ(11–11′)DPPZ}RuL2]4+ (where L = 1,10-phenanthroline or 2,2′-bipyridyl, DPPZ(11–11′)DPPZ = 11,11′-bi(dipyrido-[3,2-a:2′,3′-c]-phenazinyl)), a heterodinuclear (Ru,Co) metal polypyridyl complex [(phen)2Ru{DPPZ(11–11′)DPPZ}Co(phen)2]5+ (phen = 1,10-phenanthroline) has been designed and synthesized. A comparative study on the interaction of the complex with calf thymus DNA and yeast tRNA was investigated by UV-visible spectroscopy, fluorescence spectroscopy and viscosity measurements, as well as equilibrium dialysis and circular dichroism. The antitumour activities of the complex were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraazolium bromide method and Giemsa staining experiment. These results indicate that the configuration and structures of nucleic acids have significant effects on the binding behaviours of metal complexes. Furthermore, the complex shows different antitumour activities against selected tumour cell lines, and can cause cell apoptosis.

Synthesis of Water-Soluble Phthalocyanine Derivatives as Models for Petroleum Fractions

2014 ◽  
Vol 926-930 ◽  
pp. 266-269
Author(s):  
Ying Hui Bian ◽  
Shao Tang Xu ◽  
Le Chun Song ◽  
Dao Hong Xia
Keyword(s):  
Absorption Spectra ◽  
Water Soluble ◽  
The Self ◽  
The Novel ◽  
Model Compounds ◽  
Petroleum Fractions ◽  
Association Behavior ◽  
Uv Visible ◽  
Phthalocyanine Derivative ◽  
Self Association

As model compounds to petroleum fractions, the novel phthalocyanine derivative was synthesized and characterized. The self-association behavior of the phthalocyanine derivatives in water-DMSO were investigated by UV-visible (UV-vis) absorption spectra.

Fluorescent Rosette Nanotubes from the C-analogue of the Guanine–Cytosine (G∧C) Motif

2015 ◽  
Vol 1796 ◽  
pp. 1-6 ◽  
Author(s):  
Belete Legesse ◽  
Jae-Young Cho ◽  
Rachel L. Beingessner ◽  
Takeshi Yamazaki ◽  
Hicham Fenniri
Keyword(s):  
Electron Microscopy ◽  
Self Assembly ◽  
The Self ◽  
Visible Spectroscopy ◽  
Fluorescent Properties ◽  
Force Microscopy ◽  
Rosette Nanotubes ◽  
Atomic Force ◽  
Transmission Electron ◽  
Uv Visible

ABSTRACTRosette nanotubes (RNTs) are tubular architectures generated through the hierarchical self-assembly of the guanine-cytosine (G∧C) motif 1 or 2 (Figure 1). Motif 2 differs from 1 by the substitution at the N-atom in the G-ring with a C-atom as shown in red. In this paper, we prepare a new tricyclic G∧C base 3 from a functionalized derivative of 2 and demonstrate its self-assembly into fluorescent helical RNTs in N,N-dimethylformamide (DMF). The self-assembly and fluorescent properties of RNTs 3 were established using scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM) and UV-visible spectroscopy.

Interaction of diatomic ligands with Fe(II) meso-mono-4-pyridyl-tri-phenyl-porphyrinate. Spectral evidence of self-assembly in sublimed layers

2003 ◽  
Vol 07 (09) ◽  
pp. 623-629 ◽  
Author(s):  
Tigran S. Kurtikyan ◽  
J. Steven Ogden ◽  
Robert K. Kazaryan ◽  
Valery N. Madakyan
Keyword(s):  
Low Temperature ◽  
Self Assembly ◽  
Thin Layers ◽  
The Self ◽  
Ambient Conditions ◽  
Visible Spectroscopy ◽  
Pyridyl Group ◽  
Sublimed Layers ◽  
Uv Visible ◽  
Adjacent Molecule

The interaction of CO , NO and O2 gases with thin layers of meso-mono-4-pyridyl-tri-phenylporphyrinatoiron(II) ( FeMPyTPP ) obtained by sublimation onto low-temperature (T = 77 K ) substrates has been investigated by means of IR and UV-visible spectroscopy. In contrast to the closely-related meso-tetraphenylporphyrinatoiron(II) ( FeTPP ), the formation of two types of axial complexes has been observed. In one of these the 5th coordination site is occupied by the pyridyl group of an adjacent molecule indicating the self-assembly of Fe ( MPyTPP ) in layers with formation of coordinatively linked oligomers. The degree of oligomerisation depends on the nature of the interacting gas. Due to the specificity of the supramolecular structure, the layers are fairly stable in ambient conditions and conserve their microporosity to bind reversibly with the aforementioned ligands.

scholarly journals UV-Visible Spectrophotometric Method and Validation of Organic Compounds

2018 ◽  
Vol 3 (3) ◽  
pp. 8 ◽  
Author(s):  
Abdul Moiz Mohammed
Keyword(s):  
Absorption Coefficient ◽  
Organic Compounds ◽  
Simple Technique ◽  
Inorganic Compounds ◽  
Visible Region ◽  
Ultra Violet ◽  
Intrinsic Property ◽  
Spectroscopic Technique ◽  
Molar Absorption Coefficient ◽  
Uv Visible

Absorption and emission of radiation by a compound at different wavelengths in visible and ultra violet region forms an analytical tool for identification of organic compounds. Researchers and scientists from different fields of chemistry and life sciences are constantly in search of an alternate ways to analyze the chemical reactions involving organic and inorganic compounds. The UV-Visible spectroscopic technique serves as an important and simple technique in finding concentration of molecules in a solution, identification the functional groups identification of conjugated organic compounds. The molar absorption coefficient is an intrinsic property of species, and may be treated as a fingerprint to an organic compound. This papers presents a systematic and detailed validation of organic compounds with the molar absorption coefficient in UV-visible region.

Self-Association of the Macromolecules of Aldrich Humic Acid in Aqueous Solutions Using Ultraviolet–Visible (UV–Vis) Absorption Spectroscopy

2019 ◽  
Vol 73 (7) ◽  
pp. 810-815
Author(s):  
Nikolay L. Lavrik ◽  
Nikolay M. Bazhin
Keyword(s):  
Aqueous Solutions ◽  
Absorption Spectroscopy ◽  
Spectral Dependence ◽  
The Self ◽  
Dimerization Constant ◽  
Absorption Bands ◽  
Characteristic Absorption ◽  
Mathematical Algorithm ◽  
The Difference ◽  
Self Association

The ultraviolet–visible (UV–Vis) spectra of the aqueous solutions of humic acids (HA) Aldrich were obtained within the concentration range 1–20 mg/L (0.1–2ċ10–6 mol/L). A conclusion on the existence of the self-association (dimer formation) of HA macromolecules is based on the deviation of the concentration dependence of optical density (OD) from the linear one at [HA] > 10 mg/L (>10–6 mol/L). An original, mathematical algorithm is proposed to determine a dimerization constant K and a molar coefficient of dimer absorption [Formula: see text] The value of K was (2.56 ± 0.07) ċ 106 L/mol. The calculated molar coefficients of HA macromolecule dimer absorption, [Formula: see text], indicate an increase in the dimer extinction coefficient [Formula: see text] compared to the double value of the molar monomer absorption 2[Formula: see text]. It was established that the spectral dependence of the value β = [Formula: see text]/[Formula: see text] exhibits a minimum within a wavelength range of 300–450 nm, which is due to the difference in the efficiency of the interaction of various sites upon HA macromolecule dimerization. Thus, an approach of studying the processes of self-association is proposed using the method of UV–Vis absorption spectroscopy. This method is implemented for molecules that do not have characteristic absorption bands. The proposed method can also be successfully applied to molecules with characteristic absorption bands.

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