Nucleic Acid (Calf Thymus-DNA, Yeast tRNA) Binding and Cytotoxic Properties of a Dinuclear (Ru,Co) Metal Polypyridyl Complex

2010 ◽  
Vol 63 (10) ◽  
pp. 1453 ◽  
Author(s):  
Xi-Ling Liang ◽  
Li-Feng Tan
Keyword(s):  
Nucleic Acid ◽  
Equilibrium Dialysis ◽  
Calf Thymus Dna ◽  
Visible Spectroscopy ◽  
Yeast Trna ◽  
Polypyridyl Complex ◽  
Calf Thymus ◽  
Tumour Cell Lines ◽  
Uv Visible ◽  
Trna Binding

Based on [L2Ru{DPPZ(11–11′)DPPZ}RuL2]4+ (where L = 1,10-phenanthroline or 2,2′-bipyridyl, DPPZ(11–11′)DPPZ = 11,11′-bi(dipyrido-[3,2-a:2′,3′-c]-phenazinyl)), a heterodinuclear (Ru,Co) metal polypyridyl complex [(phen)2Ru{DPPZ(11–11′)DPPZ}Co(phen)2]5+ (phen = 1,10-phenanthroline) has been designed and synthesized. A comparative study on the interaction of the complex with calf thymus DNA and yeast tRNA was investigated by UV-visible spectroscopy, fluorescence spectroscopy and viscosity measurements, as well as equilibrium dialysis and circular dichroism. The antitumour activities of the complex were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraazolium bromide method and Giemsa staining experiment. These results indicate that the configuration and structures of nucleic acids have significant effects on the binding behaviours of metal complexes. Furthermore, the complex shows different antitumour activities against selected tumour cell lines, and can cause cell apoptosis.

scholarly journals Binding of the Bi (III) Complex of Naringin with β-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Sameena Yousuf ◽  
Israel V. Muthu Vijayan Enoch
Keyword(s):  
Inclusion Complex ◽  
Binding Constants ◽  
Calf Thymus Dna ◽  
Visible Absorption ◽  
X Ray ◽  
Fluorescence Measurements ◽  
Infrared Scanning ◽  
Calf Thymus ◽  
Fluorescence Characteristics ◽  
Uv Visible

Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb with β-Cyclodextrin (β-CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb with β-CD was 1 : 1 with a binding constant of 5.18 × 102 mol−1 dm3. The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence of β-CD. The binding constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.29 × 105 mol−1 dm3 and 6.89 × 104 mol−1 dm3, respectively. The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.25 × 104 mol−1 dm3 and 5.10 × 103 mol−1 dm3, respectively. The lowering of the binding affinity and the Ksv were observed for the interaction of Narb with ctDNA in the presence of β-CD.

The Investigation of the Neopluramycin � DNA System by UV-visible Spectroscopy

2008 ◽  
Vol 59 (9) ◽  
Author(s):  
Loredana Elena V�jan
Keyword(s):  
Absorption Coefficient ◽  
Binding Constant ◽  
The Self ◽  
Molar Absorption Coefficient ◽  
Water Mixture ◽  
Visible Spectroscopy ◽  
Dimerization Constant ◽  
Calf Thymus ◽  
Uv Visible ◽  
Self Association

The self-association of neopluramycin and the binding of this drug to calf thymus DNA were investigated by using UV-visible spectroscopy. This classical pluramycin antibiotic self-associates in 1:1 ethanol - water mixture. Starting from a simple dimerization model, the molar absorption coefficient of monomer, the molar absorption coefficient of dimer and the dimerization constant were determined. The binding constant of neopluramycin to DNA was determined using Wolfe and Scatchard methods.

Biocompatibility of Silver Nano Bio-conjugates from Vitis vinifera Seeds and its Major Component Resveratrol

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
R. Preethi ◽  
P. Padma
Keyword(s):  
Zeta Potential ◽  
Vitis Vinifera ◽  
Silver Ions ◽  
Seed Extract ◽  
Polydispersity Index ◽  
Potential Range ◽  
Visible Spectroscopy ◽  
Grape Seeds ◽  
X Ray ◽  
Uv Visible

The study focused on the green synthesis of silver nanobioconjugates (AgNPs) from phenolic-rich fruit source, Vitis vinifera seed extract and its major component phenolic, resveratrol respectively. Sunlight exposure for 20 minutes was the method of choice for the synthesis of AgNPs of the extract as well as the phenolic, resveratrol. The synthesized nanobioconjugates were characterized using UV-Visible spectroscopy, Transmission electron microscopy (TEM), Energy dispersive X-ray analysis (EDAX), X-ray diffraction (XRD), Polydispersity index, Zeta potential and Fourier transform infrared spectroscopy (FTIR). The reduction of silver ions was confirmed by UV-visible spectroscopy with peaks at 440nm for both nanobioconjugates synthesized from seed extract and compound. The nanobioconjugates showed the spherical in shape with 14-35nm in size and crystalline in nature. The conjugates are well dispersed with 0.301 and 0.287 polydispersity index and the zeta potential range at -13.6 and -14.3mV for stability. The FTRI data proved that the components in grape seeds act as good reductants and stabilizers for the silver nanobioconjugate synthesis. All the synthesized nanobioconjugates exhibited steady and sustained release of the medicinal components conjugated, proving their druggability, and were biocompatible with human cells, demonstrating their safety. The findings of the study validate the anticancer properties of silver nanobioconjugates of Vitis vinifera and its active component resveratrol.

Evaluation of Antibacterial and Antidiabetic Potential of Silver Nanoparticles using Eugenia Uniflora L. Seed Extract

2020 ◽  
Vol 13 (6) ◽  
pp. 5217-5225
Author(s):  
Guru Kumar Dugganaboyana ◽  
Chethankumar Mukunda ◽  
Suresh Darshini Inakanally
Keyword(s):  
Biomedical Applications ◽  
Pathogenic Bacteria ◽  
Seed Extract ◽  
Drug Formulation ◽  
Visible Spectroscopy ◽  
Plant Materials ◽  
X Ray ◽  
Eugenia Uniflora ◽  
Uv Visible

In recent years, green nanotechnology-based approaches using plant materials have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the seed extract of the Eugenia uniflora L. (E.uniflora). Characterization was done using UV-Visible spectroscopy, X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV-Visible spectroscopy (at 466 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in seed of E.uniflora extract accountable for the reduction of Ag+ ion and the stabilization of AgNPs was investigated. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AgNPs were evaluated for their antibacterial and along with their antidiabetic potential. The results showed that AgNPs are extremely effective with potent antidiabetic potential at a very low concentration. It also exhibited potential antibacterial activity against the three tested human pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for in vitro evaluation of their antibacterial and antidiabetic effects with potent biomedical applications.

Molecular Transformations in DNA under the Influence of UV-radiation

2020 ◽  
Vol 04 ◽  
Author(s):  
Vigen G. Barkhudaryan ◽  
Gayane V. Ananyan ◽  
Nelli H. Karapetyan
Keyword(s):  
Uv Radiation ◽  
Uv Irradiation ◽  
Absorption Spectroscopy ◽  
Calf Thymus Dna ◽  
Dilute Solutions ◽  
Concentrated Solutions ◽  
Buffer Solution ◽  
Low Concentration ◽  
Calf Thymus ◽  
Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

Ce(IV)-mediated formation of benzenediazonium ion from a non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) and its binding to DNA

1989 ◽  
Vol 54 (7) ◽  
pp. 2021-2026
Author(s):  
Marie Stiborová ◽  
Befekadu Asfaw ◽  
Pavel Anzenbacher
Keyword(s):  
Azo Dyes ◽  
Acidic Medium ◽  
Model System ◽  
Calf Thymus Dna ◽  
Suitable Model ◽  
Principal Product ◽  
Calf Thymus ◽  
Sudan I ◽  
A Minor

Ce(IV) ions in acidic medium convert a carcinogenic non-aminoazo dye, 1-phenylazo-2-hydroxy-naphthalene (Sudan I) into an ultimate carcinogen, which binds to calf thymus DNA. The principal product of Sudan I oxidation by the Ce(IV) system is the benzenediazonium ion. A minor product is the dihydroxyderivative of Sudan I, 1-(4-hydroxyphenylazo)-2,6-dihydroxynaphthalene. Other minor coloured products (yellow and brown) were not identified. The principal product (the benzenediazonium ion) is responsible for the carcinogenicity of Sudan I, as it covalently binds to DNA. Ce(IV) ions in acidic medium represent a suitable model system, which imitates the activation route of carcinogenic azo dyes.

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