Characterization of the Interaction of Amphotericin B with Cholesteryl Trifluoromethylphenyl-Carbamate by UV-Visible Spectroscopy
Amphotericin B, a polyene antibiotic, self-associates in ethanol. The self-association of antibiotic has characterized by UV-visible spectroscopy. Starting from a simple dimerization model and using different methods described in literature, one determined the molar absorption coefficient of monomer, the molar absorption coefficient of dimer and the dimerization constant. The binding of amphotericin B to cholesteryl trifluoromethylphenyl-carbamate has investigated using absorbance measurements and the results have rationalized in terms of literature models, taking into account both 1:1 drug-sterol system and cooperativity effects. The binding constant of amphotericin B to cholesteryl trifluoromethylphenyl-carbamate has determined using Benesi-Hildebrand, Scott and Scatchard methods.