The Influence of Some Sources of Nitrogen on the Growth and Development of the Phomopsis Incarcerata Pathogen (SACC.) Hohnel

2019 ◽  
Vol 70 (11) ◽  
pp. 4000-4002
Keyword(s):  
Growth And Development ◽  
Culture Media ◽  
Nitrogen Sources ◽  
Potato Dextrose Agar ◽  
Malt Extract ◽  
Beta Alanine ◽  
Ph Values ◽  
Ecological Aspects ◽  
Optimum Ph

Phomopsis incarcerata, known as the pathogen which caused the dieback of Rosa branches was detected in many orchards in Romania. Our investigations have approached a series of bio-ecological aspects of this pathogen: isolation, purification and obtaining the pathogen; determination and identification of the pathogen; establishing in vitro parameters of fungal development (nitrogen source). The isolate used in this study was obtained from Rosa spp. branches and was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and water agar which included five amino-acids: cysteine, glycine, beta-alanine, leucine and tryptophan. Leucine and glycine were favorable for the dynamics of the fungus.When the fungus grew on water agar, the sporulation was completely inhibited. The optimum pH values for the growth and creation of the Phomopsis incarcerata are in the range of 4.4-7, so weak to neutral acid. Keywords: Phomopsis, nitrogen sources, Rosa, pathogen

scholarly journals The Influence of Some Sources of Nitrogen on the Growth and Development of the Phomopsis Incarcerata Pathogen (SACC.) Hohnel

2019 ◽  
Vol 70 (11) ◽  
pp. 4000-4002
Author(s):  
Cristina Mihaescu ◽  
Madalina Militaru ◽  
Madalina Butac
Keyword(s):  
Growth And Development ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
Malt Extract ◽  
Fungal Development ◽  
Beta Alanine ◽  
Ph Values ◽  
Ecological Aspects ◽  
Optimum Ph

Phomopsis incarcerata, known as the pathogen which caused the dieback of Rosa branches was detected in many orchards in Romania. Our investigations have approached a series of bio-ecological aspects of this pathogen: isolation, purification and obtaining the pathogen; determination and identification of the pathogen; establishing in vitro parameters of fungal development (nitrogen source). The isolate used in this study was obtained from Rosa spp. branches and was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and water agar which included five amino-acids: cysteine, glycine, beta-alanine, leucine and tryptophan. Leucine and glycine were favorable for the dynamics of the fungus.When the fungus grew on water agar, the sporulation was completely inhibited. The optimum pH values for the growth and creation of the Phomopsis incarcerata are in the range of 4.4-7, so weak to neutral acid.

scholarly journals Mycelial growth and sporulation of Apoharknessia eucalyptorum on different culture media

2019 ◽  
Vol 45 (3) ◽  
pp. 272-278
Author(s):  
Alexandre Techy de Almeida Garrett ◽  
Álvaro Figueredo Santos ◽  
Afonso Figueiredo Filho ◽  
Evandro Vagner Tambarussi ◽  
Arthur Birais Gonçalves ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
In Vitro Cultivation ◽  
Malt Extract ◽  
Optimal Conditions ◽  
Southern Brazil ◽  
Eucalyptus Dunnii ◽  
White Mycelium

ABSTRACT Apoharknessia eucalyptorum was first described in 2017 and identified on leaves of Eucalyptus dunnii in Southern Brazil. However, information about in vitro cultivation for complementary studies is lacking. In the present study, leaves of E. dunnii were inoculated to demonstrate the pathogenicity of A. eucalyptorum, and growth and sporulation were evaluated at temperatures of 15, 20, and 25°C on four culture media: malt extract agar (MEA); potato dextrose agar (PDA); V8 juice agar (V8); and bean dextrose agar (BEAN), under constant lighting. Apoharknessia eucalyptorum caused leaf blight on the inoculated leaves. The best conditions for mycelial growth were at 25°C on PDA, BEAN and MEA. Considering sporulation, optimal conditions were 25°C for all tested media and 20°C for PDA and BEAN. Colony characteristics changed with temperature; at 15°C colonies formed a fluffy mycelium, whereas at 25°C mycelium spread across the medium forming dark margins lined by dirty-white mycelium and conidia. The conditions indicated for in vitro growth and sporulation of A. eucalyptorum are the culture media MEA, PDA and BEAN at 25°C

scholarly journals In vitro evaluation of fungicide and bio-fungicides against isolates of Alternaria solani Sorauer

2021 ◽  
Vol 26 (1) ◽  
pp. 2295-2301
Author(s):  
CRISTINA MIHAESCU ◽  
MONICA STURZEANU ◽  
ALIN DIN
Keyword(s):  
Early Blight ◽  
Culture Media ◽  
Alternaria Solani ◽  
Colony Growth ◽  
Potato Dextrose Agar ◽  
Malt Extract ◽  
Mycelia Growth ◽  
Growth Of Fungi ◽  
High Inhibition

In vitro control of Alternaria solani included studies of different fungicides, essential oils basil and ethyl extract of Allium cepa. In Romania in the case of tomato culture it is very low due to several production constrains including diseases. One such disease is the early blight caused by fungus Alternaria solani (Ell.) Martin. The isolate used in this study was obtained from tomato and it was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and Czapek Dox medium. Using chlorothalonil, thiophanate methyl, prochloraz, tebuconazole and cooper sulphate, they were evaluated in order to control colony growth of fungi, at the concentrations recommended in agriculture by the specialized literature. The extract of Allium concentration with three levels (3%, 75% and 100%) significantly stronger effect on reducing mycelia growth, reducing spore germination and causing high inhibition percentage of A. solani. The fungal effect of the essential oil basil is very low being close to that of the control.

Trans-sialidase Associated with Atherosclerosis: Defining the Identity of a Key Enzyme Involved in the Pathology

2019 ◽  
Vol 20 (9) ◽  
pp. 938-941
Author(s):  
Victor Y. Glanz ◽  
Veronika A. Myasoedova ◽  
Andrey V. Grechko ◽  
Alexander N. Orekhov
Keyword(s):  
Acid Metabolism ◽  
Research Subject ◽  
Disease Pathogenesis ◽  
Sialidase Activity ◽  
Ph Values ◽  
Ldl Particles ◽  
Optimum Ph ◽  
Key Enzyme ◽  
St6gal I

Atherosclerosis is associated with the increased trans-sialidase activity, which can be detected in the blood plasma of atherosclerosis patients. The likely involvement in the disease pathogenesis made this activity an interesting research subject and the enzyme that may perform such activity was isolated and characterized in terms of substrate specificity and enzymatic properties. It was found that the enzyme has distinct optimum pH values, and its activity was enhanced by the presence of Ca2+ ions. Most importantly, the enzyme was able to cause atherogenic modification of lowdensity lipoprotein (LDL) particles in vitro. However, the identity of the discovered enzyme remained to be defined. Currently, sialyltransferases, mainly ST6Gal I, are regarded as major contributors to sialic acid metabolism in human blood. In this mini-review, we discuss the possibility that atherosclerosis- associated trans-sialidase does, in fact, belong to the sialyltransferases family.

scholarly journals The Effect of pH Stress on the Survival of Rhizobium Trifolii and Sinorhizobium Meliloti in Vitro

2013 ◽  
Vol 70 (2) ◽  
pp. 449-450
Author(s):  
Monica NISTE ◽  
Roxana VIDICAN ◽  
Ioan ROTAR ◽  
Rodica POP
Keyword(s):  
Sinorhizobium Meliloti ◽  
Agar Medium ◽  
Rhizobium Trifolii ◽  
Ph Values ◽  
Optimum Ph ◽  
Yeast Extract Mannitol ◽  
Rhizobial Species ◽  
The Impact ◽  
Different Soils

Nitrogen-fixing symbiotic bacteria known as rhizobia can exist in different soils and adapt to different environmental conditions. The aim of this study was to determine the impact of pH on the growth of Rhizobium trifolii and Sinorhizobium meliloti. Rhizobial species were isolated using yeast extract mannitol agar medium) in which the pH values were adjusted to 5.0, 6.0, 8.0 and 9.0 by adding HCl and NaOH. The optimum pH for rhizobia is neutral or slightly alkaline (pH 8) and they are more sensitive to acidity. Sinorhizobium meliloti developed better in an acid medium compared to Rhizobium trifolii.

scholarly journals DETERMINATION OF OPTIMUM CULTURE CONDITIONS FOR MYCELIAL GROWTH OF Macrolepiota procera MUSHROOM

2020 ◽  
Vol 19 (1) ◽  
pp. 11-20
Author(s):  
Aysun Pekşen ◽  
Beyhan Kibar
Keyword(s):  
Yeast Extract ◽  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Malt Extract ◽  
The Public ◽  
Carbon And Nitrogen ◽  
Ph Values ◽  
Incubation Temperatures ◽  
Macrolepiota Procera

Macrolepiota procera, commonly called the Parasol Mushroom, is a delicious mushroom collected from the nature and commonly consumed by the public in many regions of Turkey. This study was conducted to determine the optimum culture conditions (pH, temperature, carbon and nitrogen sources) for mycelial growth of M. procera. Three pH values (pH 5.0, 5.5 and 6.0), four incubation temperatures (15, 20, 25 and 30°C), seven carbon (C) sources (dextrose, glucose, lactose, maltose, mannitol, sucrose and xylose) and six nitrogen (N) sources ((NH4)2HPO4, NH4NO3 and Ca(NO3)2, malt extract, peptone and yeast extract) were investigated. In the second step of the study, the effect of seven pH values (4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 7.0) on the mycelial colony diameter was examined at 20 and 25°C since these temperatures gave the best mycelial growth in the previously conducted temperature experiment. The best mycelial growth was determined at pH 6.0. The optimum temperature for mycelial growth of M. procera was found as 25°C. The use of glucose as carbon source and yeast extract and peptone as nitrogen source in the culture medium gave the best results for mycelial growth. Determining of optimum culture conditions for mycelial growth of M. procera will provide important contributions to the fortcoming studies on it’s commercially cultivation in Turkey.

scholarly journals Role of ascorbic acid in promoting follicle integrity and survival in intact mouse ovarian follicles in vitro

Reproduction ◽  
2001 ◽  
pp. 89-96 ◽  
Author(s):  
AA Murray ◽  
MD Molinek ◽  
SJ Baker ◽  
FN Kojima ◽  
MF Smith ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Basement Membrane ◽  
Growth And Development ◽  
Culture Media ◽  
Membrane Integrity ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Follicular Growth ◽  
Intact Mouse

Ascorbic acid has three known functions: it is necessary for collagen synthesis, promotes steroidogenesis and acts as an antioxidant. Within the ovary, most studies have concentrated on the role of ascorbic acid in luteal formation and regression and little is known about the function of this vitamin in follicular growth and development. Follicular growth and development were investigated in this study using an individual follicle culture system that allows the growth of follicles from the late preantral stage to Graafian morphology. Follicles were isolated from prepubertal mice and cultured for 6 days. Control media contained serum and human recombinant FSH. Further groups of follicles were cultured in the same media but with the addition of ascorbic acid at concentrations of either 28 or 280 micromol l(-1). Addition of ascorbic acid at the higher concentration significantly increased the percentage of follicles that maintained basement membrane integrity throughout culture (P < 0.001). Ascorbic acid had no effect on the growth of the follicles or on oestradiol production. Metalloproteinase 2 activity tended to increase at the higher concentration of ascorbic acid and there was a significant concomitant increase in the activity of tissue inhibitor of metalloproteinase 1 (P < 0.01). Follicles cultured without the addition of serum but with FSH and selenium in the culture media underwent apoptosis. Addition of ascorbic acid to follicles cultured under serum-free conditions significantly reduced apoptosis (P < 0.05). From these data it is concluded that ascorbic acid is necessary for remodelling the basement membrane during follicular growth and that the ability of follicles to uptake ascorbic acid confers an advantage in terms of granulosa cell survival.

scholarly journals Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

2020 ◽  
Vol 50 (1) ◽  
pp. 61-67
Author(s):  
Marcos Diones Ferreira SANTANA ◽  
Ruby VARGAS-ISLA ◽  
Janaina da Costa NOGUEIRA ◽  
Thiago ACCIOLY ◽  
Bianca Denise Barbosa da SILVA ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Brazilian Amazon ◽  
Potato Dextrose Agar ◽  
Morphological Description ◽  
Agar Culture ◽  
Biotechnological Potential ◽  
Sexual Crosses ◽  
High Diversity

ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.

scholarly journals Effect of Nutritive Media and PH on in vitro Mycelial Growth of some Pleurotus eryngii Strains

2016 ◽  
Vol 73 (2) ◽  
pp. 276
Author(s):  
Alexandru Valentin Zagrean ◽  
Gicuta Sbirciog ◽  
Mihaela Alina Buzatu ◽  
Iuliana Mandru
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Weak Acid ◽  
Pleurotus Eryngii ◽  
Mycelium Growth ◽  
Ph Values ◽  
Mycelial Growth Rate ◽  
Initial Ph ◽  
Optimal Values

The mycelial growth rate of 4 strains of Pleurotus eryngii was first studied on different culture media - MEA, PDA and WEA (wheat extract agar). In the next experiment, we checked the mycelial growth on the same medium but at 3 different pHs. MEA medium ensured the highest growth rates for all the strains under test, followed by PDA. Pery-G strain showed the fastest growing rate on MEA (5.79 mm day-1) and PDA (3.04 mm day-1), respectively. Test concerning the mycelium growth on PDA at different initial pH values (5.5, 6.0, 6.5) showed the preference of our P.eryngii strains for media with weak acid to the neutral reaction , the optimal values being 6.0. There were no big differences among mycelium growing rates of the same strain for the three pH variants

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