scholarly journals The Effect of pH Stress on the Survival of Rhizobium Trifolii and Sinorhizobium Meliloti in Vitro

2013 ◽  
Vol 70 (2) ◽  
pp. 449-450
Author(s):  
Monica NISTE ◽  
Roxana VIDICAN ◽  
Ioan ROTAR ◽  
Rodica POP
Keyword(s):  
Sinorhizobium Meliloti ◽  
Agar Medium ◽  
Rhizobium Trifolii ◽  
Ph Values ◽  
Optimum Ph ◽  
Yeast Extract Mannitol ◽  
Rhizobial Species ◽  
The Impact ◽  
Different Soils

Nitrogen-fixing symbiotic bacteria known as rhizobia can exist in different soils and adapt to different environmental conditions. The aim of this study was to determine the impact of pH on the growth of Rhizobium trifolii and Sinorhizobium meliloti. Rhizobial species were isolated using yeast extract mannitol agar medium) in which the pH values were adjusted to 5.0, 6.0, 8.0 and 9.0 by adding HCl and NaOH. The optimum pH for rhizobia is neutral or slightly alkaline (pH 8) and they are more sensitive to acidity. Sinorhizobium meliloti developed better in an acid medium compared to Rhizobium trifolii.

Trans-sialidase Associated with Atherosclerosis: Defining the Identity of a Key Enzyme Involved in the Pathology

2019 ◽  
Vol 20 (9) ◽  
pp. 938-941
Author(s):  
Victor Y. Glanz ◽  
Veronika A. Myasoedova ◽  
Andrey V. Grechko ◽  
Alexander N. Orekhov
Keyword(s):  
Acid Metabolism ◽  
Research Subject ◽  
Disease Pathogenesis ◽  
Sialidase Activity ◽  
Ph Values ◽  
Ldl Particles ◽  
Optimum Ph ◽  
Key Enzyme ◽  
St6gal I

Atherosclerosis is associated with the increased trans-sialidase activity, which can be detected in the blood plasma of atherosclerosis patients. The likely involvement in the disease pathogenesis made this activity an interesting research subject and the enzyme that may perform such activity was isolated and characterized in terms of substrate specificity and enzymatic properties. It was found that the enzyme has distinct optimum pH values, and its activity was enhanced by the presence of Ca2+ ions. Most importantly, the enzyme was able to cause atherogenic modification of lowdensity lipoprotein (LDL) particles in vitro. However, the identity of the discovered enzyme remained to be defined. Currently, sialyltransferases, mainly ST6Gal I, are regarded as major contributors to sialic acid metabolism in human blood. In this mini-review, we discuss the possibility that atherosclerosis- associated trans-sialidase does, in fact, belong to the sialyltransferases family.

The Influence of Some Sources of Nitrogen on the Growth and Development of the Phomopsis Incarcerata Pathogen (SACC.) Hohnel

2019 ◽  
Vol 70 (11) ◽  
pp. 4000-4002
Keyword(s):  
Growth And Development ◽  
Culture Media ◽  
Nitrogen Sources ◽  
Potato Dextrose Agar ◽  
Malt Extract ◽  
Beta Alanine ◽  
Ph Values ◽  
Ecological Aspects ◽  
Optimum Ph

Phomopsis incarcerata, known as the pathogen which caused the dieback of Rosa branches was detected in many orchards in Romania. Our investigations have approached a series of bio-ecological aspects of this pathogen: isolation, purification and obtaining the pathogen; determination and identification of the pathogen; establishing in vitro parameters of fungal development (nitrogen source). The isolate used in this study was obtained from Rosa spp. branches and was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and water agar which included five amino-acids: cysteine, glycine, beta-alanine, leucine and tryptophan. Leucine and glycine were favorable for the dynamics of the fungus.When the fungus grew on water agar, the sporulation was completely inhibited. The optimum pH values for the growth and creation of the Phomopsis incarcerata are in the range of 4.4-7, so weak to neutral acid. Keywords: Phomopsis, nitrogen sources, Rosa, pathogen

The effect of rhizobiophages on populations of Rhizobium trifolii in the root zone of clover plants

1980 ◽  
Vol 26 (5) ◽  
pp. 572-576 ◽  
Author(s):  
Yvonne M. Barnet
Keyword(s):  
Root Zone ◽  
Agar Medium ◽  
Trifolium Subterraneum ◽  
Dry Weight ◽  
Susceptible Strain ◽  
Rhizobium Trifolii ◽  
Single Strain

The effect of rhizobiophages CT3 and CT4 on Rhizobium trifolii SU91 and SU36, respectively, was examined. These phage-susceptible strains were used, either singly or paired with a competing phage-resistant type as inoculum for Trifolium subterraneum plants growing in vitro in agar medium. Addition of bacteriophage to single strain experiments produced no significant effects on total bacterial numbers, nodulation, or plant dry weight. However, when phages were added to the paired inocula, significant decreases in counts of the susceptible strain were observed, frequently accompanied by a rise in numbers of the resistant type. An increase in the proportion of ineffective variants of the susceptible rhizobia was also observed, although plant dry weights and nodule numbers were not altered. These effects were dependent only upon a difference in phage susceptibility of the bacteria and were obtained even when the competing strain was only partially resistant and a relatively poor competitor.

scholarly journals The Influence of Some Sources of Nitrogen on the Growth and Development of the Phomopsis Incarcerata Pathogen (SACC.) Hohnel

2019 ◽  
Vol 70 (11) ◽  
pp. 4000-4002
Author(s):  
Cristina Mihaescu ◽  
Madalina Militaru ◽  
Madalina Butac
Keyword(s):  
Growth And Development ◽  
Culture Media ◽  
Potato Dextrose Agar ◽  
Malt Extract ◽  
Fungal Development ◽  
Beta Alanine ◽  
Ph Values ◽  
Ecological Aspects ◽  
Optimum Ph

Phomopsis incarcerata, known as the pathogen which caused the dieback of Rosa branches was detected in many orchards in Romania. Our investigations have approached a series of bio-ecological aspects of this pathogen: isolation, purification and obtaining the pathogen; determination and identification of the pathogen; establishing in vitro parameters of fungal development (nitrogen source). The isolate used in this study was obtained from Rosa spp. branches and was cultivated on three culture media: potato dextrose agar (PDA), malt extract agar and water agar which included five amino-acids: cysteine, glycine, beta-alanine, leucine and tryptophan. Leucine and glycine were favorable for the dynamics of the fungus.When the fungus grew on water agar, the sporulation was completely inhibited. The optimum pH values for the growth and creation of the Phomopsis incarcerata are in the range of 4.4-7, so weak to neutral acid.

Effects of incubation fluid pH and fibrolytic enzymes on the in vitro fermentation of pure substrates, assessed using the Reading Pressure Technique (RPT)

2002 ◽  
Vol 2002 ◽  
pp. 208-208 ◽  
Author(s):  
D. Colombatto ◽  
F. L. Mould ◽  
M. K. Bhat ◽  
E. Owen
Keyword(s):  
In Vitro Fermentation ◽  
Commercial Enzyme ◽  
Fibrolytic Enzymes ◽  
Optimum Activity ◽  
Ph Values ◽  
Optimum Ph ◽  
Ph Conditions ◽  
Ph Range ◽  
Optimum Ph Range

Modern feeding practices often lead to ruminal conditions being sub-optimal for fibre digestion. It has been speculated that fibrolytic enzymes, which usually show optimum activity at pH values below 6.0, may be of benefit when applied to diets of high producing animals. This study used a commercial enzyme mixture (EM), already identified as effective; to investigate its optimum pH range with respect to activity and its impact on the fermentation profiles of pure substrates, under differing pH conditions.

The Predominant microRNAs in β-cell Clusters for Insulin Regulation and Diabetic Control

2020 ◽  
Vol 21 (7) ◽  
pp. 722-734
Author(s):  
Adele Soltani ◽  
Arefeh Jafarian ◽  
Abdolamir Allameh
Keyword(s):  
Mirna Expression ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Diabetic Control ◽  
In Vitro Proliferation ◽  
Cell Functions ◽  
Mirna Expression Profiles ◽  
Multiple Processes ◽  
The Impact

micro (mi)-RNAs are vital regulators of multiple processes including insulin signaling pathways and glucose metabolism. Pancreatic β-cells function is dependent on some miRNAs and their target mRNA, which together form a complex regulative network. Several miRNAs are known to be directly involved in β-cells functions such as insulin expression and secretion. These small RNAs may also play significant roles in the fate of β-cells such as proliferation, differentiation, survival and apoptosis. Among the miRNAs, miR-7, miR-9, miR-375, miR-130 and miR-124 are of particular interest due to being highly expressed in these cells. Under diabetic conditions, although no specific miRNA profile has been noticed, the expression of some miRNAs and their target mRNAs are altered by posttranscriptional mechanisms, exerting diverse signs in the pathobiology of various diabetic complications. The aim of this review article is to discuss miRNAs involved in the process of stem cells differentiation into β-cells, resulting in enhanced β-cell functions with respect to diabetic disorders. This paper will also look into the impact of miRNA expression patterns on in vitro proliferation and differentiation of β-cells. The efficacy of the computational genomics and biochemical analysis to link the changes in miRNA expression profiles of stem cell-derived β-cells to therapeutically relevant outputs will be discussed as well.

The Impact of Antioxidants from the Diet on Breast Cancer Cells Monitored by Raman Microspectroscopy

2018 ◽  
Vol 16 (2) ◽  
pp. 127-137
Author(s):  
Paula Sofia Coutinho Medeiros ◽  
Ana Lúcia Marques Batista de Carvalho ◽  
Cristina Ruano ◽  
Juan Carlos Otero ◽  
Maria Paula Matos Marques
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Breast Adenocarcinoma ◽  
Coumaric Acid ◽  
Human Breast ◽  
The Impact

Background: The impact of the ubiquitous dietary phenolic compound p-coumaric acid on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic analysis in cells. </P><P> Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities, depending on the concentration and cell line probed: a significant anti-invasive ability was detected for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen- dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome on the triple-negative MDA-MB-231 cell line. Results: The main impact on the cellular biochemical profile was verified to be on proteins and lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability. Conclusion: p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.

VDAC1 Mediated Anticancer Activity of Gallic Acid in Human Lung Adenocarcinoma A549 Cells

2018 ◽  
Vol 18 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Aikebaier Maimaiti ◽  
Amier Aili ◽  
Hureshitanmu Kuerban ◽  
Xuejun Li
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Gallic Acid ◽  
Molecular Mechanisms ◽  
A549 Cells ◽  
Dependent Manner ◽  
Lung Carcinoma Cells ◽  
Induced Apoptosis ◽  
The Impact

Aims: Gallic acid (GA) is generally distributed in a variety of plants and foods, and possesses cell growth-inhibiting activities in cancer cell lines. In the present study, the impact of GA on cell viability, apoptosis induction and possible molecular mechanisms in cultured A549 lung carcinoma cells was investigated. Methods: In vitro experiments showed that treating A549 cells with various concentrations of GA inhibited cell viability and induced apoptosis in a dose-dependent manner. In order to understand the mechanism by which GA inhibits cell viability, comparative proteomic analysis was applied. The changed proteins were identified by Western blot and siRNA methods. Results: Two-dimensional electrophoresis revealed changes that occurred to the cells when treated with or without GA. Four up-regulated protein spots were clearly identified as malate dehydrogenase (MDH), voltagedependent, anion-selective channel protein 1(VDAC1), calreticulin (CRT) and brain acid soluble protein 1(BASP1). VDAC1 in A549 cells was reconfirmed by western blot. Transfection with VDAC1 siRNA significantly increased cell viability after the treatment of GA. Further investigation showed that GA down regulated PI3K/Akt signaling pathways. These data strongly suggest that up-regulation of VDAC1 by GA may play an important role in GA-induced, inhibitory effects on A549 cell viability.

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