Seasonal Variation and Age-Related Changes in Semen Quality of Limousin Bull in Indonesian Artificial Insemination Center

The objective of this study was to determine the effect of season and age on semen quality (individual motility, mass motility, concentration, volume, pre-freeze motility, and post-thaw motility) of Limousin Bull under the tropical condition in Indonesian artificial insemination center. This study used a large data set recorded over eight years (2011 until 2018) of production period (n = 6758 ejaculates, n = 19 bulls) retrieved from Singosari National Artificial Insemination Center. Rainfall and other climatic data were collected from Malang Meteorological, Climatology, and Geophysics Agency, Indonesia. Seasonal effect was grouped into three groups, i.e. rainy season (total rainfall per month >200mm), humid season (total rainfall per month = 100-200 mm), and dry season (total rainfall per month <100). Bull age is grouped into seven groups (2, 3, 4, 5, 6, 7 and 8). Data were analyzed using Mixed Model procedure classifying effect of season and age on fixed effect and bull as a random effect. Season had a highly significant effect on individual motility, pre-freeze motility, and post-thaw motility (P<0.001), and did not have a significant effect on concentration, volume, pH, and mass motility. Individual motility, pre-freeze motility, and post-thaw motility were highest in the rainy season and declined in the dry season. Bull age had a highly significant effect on individual motility, concentration, volume, pre-freeze motility, and post-thaw motility (P<0,001). Bull age did not have a significant effect on mass motility and pH. Two- and three-years old bulls have superior individual motility and concentration. However, it is decreasing at age three until eight years old. The lowest volume was at age two and increased gradually until age eight. Meanwhile, pre-freeze motility and post-thaw motility increased at two to five years old and then declined until eight years old.

2021 ◽  
Vol 32 (2) ◽  
pp. 327-341
Author(s):  
N. Nwoko ◽  
S. N. Ibe

A breeding programme, which spanned over four seasons (early dry, late dry, and early rainy, late rainy), was carried with one west African Dwarf (WAD) buck, one red sokoto (RS) buck, eight WAD does and eight RS does. The buck were subjected electrical stimulation wiht an electrd-ejaculator and their semen collected and analysed.Fertility rate was significant (P<0.05) higher in WAD than in RS in early dry season. There was no significant eason effect (P,0.05). of season on fertility of WAD. Fertility was least in the early dry season in RS and the difference from other season was significant (P,0.05). The effect of season on both semen volume and concentration was significant (P,0.05). There was a progressive increase in both parameter from early dry season to late rainy season. However, RS showed superiorityover WAD in both parameters in all the seasons. There were no significant effects of breeds or season on allother parameters studied. The indication is that both breeds could be used for all-year-round breeding with adequate feeding in the zone. However, for purpose of artificial insemination, RS bucks, which produce larger quatities of semen with higher concentration, should be preferred.


Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 499
Author(s):  
Salmatta Ibrahim A ◽  
Fayyaz Ali Memon ◽  
David Butler

Ensuring a sustainable urban water supply for developing/low-income countries requires an understanding of the factors affecting water consumption and technical evidence of individual consumption which can be used to design an improved water demand projection. This paper compared dry and rainy season water sources available for consumption and the end-use volume by each person in the different income groups. The study used a questionnaire survey to gather household data for a total of 398 households, which was analysed to develop the relationship between per capita water consumption characteristics: Socio-economic status, demographics, water use behaviour around indoor and outdoor water use activities. In the per capita water consumption patterns of Freetown, a seasonal variation was found: In the rainy season, per capita water consumption was found to be about 7% higher than the consumption for the full sample, whilst in the dry season, per capita water consumption was almost 14% lower than the full survey. The statistical analysis of the data shows that the average per capita water consumption for both households increases with income for informal slum-, low-, middle- and high-income households without piped connection (73, 78, 94 and 112 L/capita/day) and with connection (91, 97, 113 and 133 L/capita/day), respectively. The collected data have been used to develop 20 statistical models using the multiple linear stepwise regression method for selecting the best predictor variable from the data set. It can be seen from the values that the strongest significant relationships of per capita consumption are with the number of occupants (R = −0.728) in the household and time spent to fetch water for use (R = −0.711). Furthermore, the results reveal that the highest fraction of end use is showering (18%), then bathing (16%), followed by toilet use (14%). This is not in agreement with many developing countries where toilet use represents the largest component of indoor end use.


Water ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 851
Author(s):  
Jamilly N. Muniz ◽  
Klinger G. Duarte ◽  
Fábio H. Ramos Braga ◽  
Neuriane S. Lima ◽  
Darlan F. Silva ◽  
...  

Watersheds are defined as a set of lands where water drainage occurs through rivers and their tributaries. A large quantity of water resources exist in the state of Maranhão, Brazil, where rivers and their basins must meet environmental quality standards defined by the limits set out in national environmental council (CONAMA) legislation 357/05 for physicochemical and microbiological parameters, including parasites. Multivariate statistical techniques were applied to study the temporal and spatial variations in water quality of a segment of the Pindaré River. The data set included nine parameters for three sampling points over eleven months. Principal component analysis grouped the monitored sampling points into four clusters and identified electrical conductivity, temperature, total dissolved solids (TDS), pH, salinity, and Escherichia coli as being associated with the dry season and nitrite, nitrate, and turbidity as being associated with the rainy season. Three principal components explained 83.80% of the data variance during the rainy and dry seasons. The evaluated correlations indicated that during the rainy season, nitrite (~0.18 mg L−1) and turbidity (~46.00 NTU) levels were the highest, but pH was at its lowest (~6.61). During the dry season, TDS (~155.00 mg L−1) and pH (~8.10) were highest, and E. coli bacteria was more abundant.


2020 ◽  
Vol 45 (4) ◽  
pp. 261-267
Author(s):  
S. Suyadi ◽  
B. Purwantara ◽  
A. Furqon ◽  
W. A. Septian ◽  
I. Novianti ◽  
...  

Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.


2021 ◽  
Vol 902 (1) ◽  
pp. 012008
Author(s):  
M H Prasetyowati ◽  
L A Pradista ◽  
N Widyas ◽  
S Prastowo

Abstract This study aims to estimate the Most Probable Producing Ability (MPPA) values of semen quality in five Bali cattle bull that collected from January to October 2016 at Singosari AI Center. The semen quality parameter consists of semen volume (ml), motility (%), concentration (x106), and total sperm (x106/ml) observed in rainy and dry season. Data were analyzed using intraclass correlation to estimate the repeatability and MPPA values. The average of semen quality in rainy and dry seasons, respectively, in volume were 3.91-6.24 ml and 4.38-6.84 ml, motility was 46.31-70.00% and 48.26-70.45%, semen concentrations were 844.78-1059.02 × 106/ml and 1033.15-1260.16 × 106/ml, and total sperm were 3280.58-5964.50 × 106 and 4493.31-7206.96×106. In this study, dry season shows better semen quality parameter as well as the repeatability value, therefore the estimation of MPPA in dry season is more accurate compared to rainy season.


2015 ◽  
Vol 31 (2) ◽  
pp. 181-192
Author(s):  
A.O. Oguntunji ◽  
O.A. Oladejo ◽  
K.L. Ayorinde

Seasonal variation is one of the principal non-genetic factors influencing performance of poultry in tropical environment. This study was conducted to investigate influence of seasonal variation on egg production and incidence of mortality in intensively-reared non-descript Muscovy ducks in Nigeria. Egg production and incidence of mortality in sixty two (62) female Muscovy ducks was studied in a 12-month trial divided into two major seasons: wet (April - September) and dry (October - March) and four sub-seasons: early rainy season (April - June), late rainy season (July - September), early dry season (October - December) and late dry season (January - March). Student?s t-test and Completely Randomized Design was used to analyse seasonal and sub-seasonal effect on performance, respectively. Season and sub-season significantly (P < 0.05) affected egg production; higher egg production was recorded in wet season compared with dry season (16.18% vs. 1.32%). Among sub-seasons, highest egg production was recorded in late rainy season (20.92%) while the least (0.00%) was obtained in late dry season. Conversely, there was no significant (P > 0.05) effect of season and sub-season on mortality. It is evident that seasonal variation had no effect on incidence of mortality but significantly affected egg production of Muscovy duck and the adverse effect is more pronounced in dry season most especially in late dry season.


2015 ◽  
Vol 4 (1) ◽  
pp. 350-355
Author(s):  
Aderson Martins Viana Neto ◽  
Maria Gorete Flores Salles ◽  
Inti Campos Salles Rodrigues ◽  
Vicente José Figueirêdo Freitas ◽  
Arlindo Alencar Araripe Noronha Moura ◽  
...  

This study aimed to evaluate the efficiency of biostimulation (Male Effect) through kidding rate of artificially inseminated goats during rainy (March-April) and dry (August-September) seasons of 2012 at Ceara, Brazil. The male effect was effective regardless of season, resulting in 94.5% of goats in estrus. The pregnancy rate for the dry season (57.6%) was proportionally higher than the rainy season (44.4%). However, the kidding rate has tended (P = 0.074) to be higher in the dry season. In general, the second estrus was more conducive to pregnancy, either to rainy or dry season. Thus, the artificial insemination of goats with estrus induced and synchronized by the male effect can be a feasible management resulting in satisfactory productive and reproductive index.


2018 ◽  
Vol 44 (1) ◽  
pp. 7
Author(s):  
Caroline De Veronez Ribeiro ◽  
Júlia Linck Moroni ◽  
Mariana Boscato Menegat ◽  
Ana Paula Gonçalves Mellagi ◽  
Mari Lourdes Bernardi ◽  
...  

Background: The extensive use of artificial insemination in the swine industry emphasizes the need for distribution of good quality semen doses by the artificial insemination centers. A factor that could modify seminal quality is the contact of extended semen with environmental air. However, only few studies have investigated the effects of air presence on semen parameters. Thus, the aim of the present study was to evaluate the influence of different amounts of air on porcine semen quality during the storage period.Materials, Methods & Results: Four ejaculates from five sexually mature boars were used. Each ejaculate (n = 20) was isothermically diluted (30 x 106/mL) in a BTS-extender and assigned to three groups differing on the amount of air entrapped within the polystyrene tubes (100 mL). In group AIR-0 the tubes were completely filled with extended semen (0% air); in group AIR-25, 75% of the tube volume was filled with extended semen and 25% with air; in group AIR-50, 50% of the tube volume was filled with extended semen. The semen doses were stored at 17.3 ± 0.5ºC for 120 h. The motility parameters were assessed at 24, 72 and 120 h of storage using a CASA system. The evaluation of acrosomal integrity was performed by examining formalin-fixed samples under phase-contrast microscopy (1000 x) at 72 and 120 h of storage. The pH was measured at 24, 48, 72, and 120 h of storage with a digital pH-meter. At 120 h, samples were incubated in waterbath at 38°C for the thermoresistance test and sperm motility was assessed after 30 and 120 min of incubation. For each time analysis, a different sealed tube was used in order to maintain a constant amount of air within the tubes during storage. Data were analyzed as repeated measures using the GLIMMIX procedure of SAS® Software. Boar and week of collection were included as random effect in the model. Groups were compared using the Tukey-Kramer test at a significance level of 5%. The pH was significantly influenced by the amount of air entrapped within the tubes (P < 0.01). Throughout the experiment, the pH of semen doses was higher as the amount of air increased within the tubes. The mean pH for groups AIR-50, AIR-25 and AIR-0 was 7.51 ± 0.06, 7.25 ± 0.06 and 7.07 ± 0.06, respectively. Overall motility was similar among the groups for up to 24 h of storage. At 72 h and 120 h of storage, total, progressive and rapid motility were higher (P < 0.05) in AIR-0 compared to AIR-50. The kinematic parameters DAP and VAP decreased as the volume of air within the tubes increased (P < 0.05). Group AIR-0 exhibited higher values (P < 0.05) for DSL, DCL, VSL, VCL, BCF, and WOB compared to AIR-25 and AIR-50. Furthermore, after the thermoresistance test performed at 120 h of storage, total motility, progressive motility and rapid motility were negatively affected (P < 0.05) by the increasing amounts of air within the tubes. There was no effect of air amount within the semen doses on acrosome integrity (P > 0.05).Discussion: The presence of air within tubes affects the quality of extended semen during storage, resulting in increase of pH and subsequent decrease in motility preservation. This pH-rise could be explained by the loss of CO2 from the diluted semen to the air compartment within the tube. At the same time, it is possible to infer that this event influences the intracellular pH of the spermatozoa, increasing sperm metabolism and, consequently, impairing sperm motility. Therefore, we conclude that avoiding air presence within storage containers is recommended in order to ensure the quality of boar semen doses.


Author(s):  
Nurul Isnaini ◽  
Tri Harsi ◽  
Devi Maharani

This study was carried out to evaluate the seasonal variation on semen characteristics of Murrah buffalo bulls in Indonesia. A total of three Murrah buffalo bulls were included in this study. The semen was collected once a week during the rainy season (from January to April and from October to December) and the dry season (from May to September). Immediately after collection, the semen volume, sperm concentration, total sperm output, individual motility, post-thawing motility, and straw production were evaluated. The results showed that the season did not change (P0.05) the semen volume, individual motility, and post-thawing motility. However, sperm concentration, total sperm output, and straw production were decreased in the dry season (P0.05) as compared to the rainy season. In conclusion, several semen characteristics of Murrah buffalo bulls may vary depending on the season of collection. The semen collection during the rainy season is more profitable than the dry season.


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