scholarly journals The Influence of Air Contact on the Quality of Extended Boar Semen

2018 ◽  
Vol 44 (1) ◽  
pp. 7
Author(s):  
Caroline De Veronez Ribeiro ◽  
Júlia Linck Moroni ◽  
Mariana Boscato Menegat ◽  
Ana Paula Gonçalves Mellagi ◽  
Mari Lourdes Bernardi ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Repeated Measures ◽  
Semen Quality ◽  
Random Effect ◽  
Semen Parameters ◽  
Swine Industry ◽  
Subsequent Decrease ◽  
Boar Semen

Background: The extensive use of artificial insemination in the swine industry emphasizes the need for distribution of good quality semen doses by the artificial insemination centers. A factor that could modify seminal quality is the contact of extended semen with environmental air. However, only few studies have investigated the effects of air presence on semen parameters. Thus, the aim of the present study was to evaluate the influence of different amounts of air on porcine semen quality during the storage period.Materials, Methods & Results: Four ejaculates from five sexually mature boars were used. Each ejaculate (n = 20) was isothermically diluted (30 x 106/mL) in a BTS-extender and assigned to three groups differing on the amount of air entrapped within the polystyrene tubes (100 mL). In group AIR-0 the tubes were completely filled with extended semen (0% air); in group AIR-25, 75% of the tube volume was filled with extended semen and 25% with air; in group AIR-50, 50% of the tube volume was filled with extended semen. The semen doses were stored at 17.3 ± 0.5ºC for 120 h. The motility parameters were assessed at 24, 72 and 120 h of storage using a CASA system. The evaluation of acrosomal integrity was performed by examining formalin-fixed samples under phase-contrast microscopy (1000 x) at 72 and 120 h of storage. The pH was measured at 24, 48, 72, and 120 h of storage with a digital pH-meter. At 120 h, samples were incubated in waterbath at 38°C for the thermoresistance test and sperm motility was assessed after 30 and 120 min of incubation. For each time analysis, a different sealed tube was used in order to maintain a constant amount of air within the tubes during storage. Data were analyzed as repeated measures using the GLIMMIX procedure of SAS® Software. Boar and week of collection were included as random effect in the model. Groups were compared using the Tukey-Kramer test at a significance level of 5%. The pH was significantly influenced by the amount of air entrapped within the tubes (P < 0.01). Throughout the experiment, the pH of semen doses was higher as the amount of air increased within the tubes. The mean pH for groups AIR-50, AIR-25 and AIR-0 was 7.51 ± 0.06, 7.25 ± 0.06 and 7.07 ± 0.06, respectively. Overall motility was similar among the groups for up to 24 h of storage. At 72 h and 120 h of storage, total, progressive and rapid motility were higher (P < 0.05) in AIR-0 compared to AIR-50. The kinematic parameters DAP and VAP decreased as the volume of air within the tubes increased (P < 0.05). Group AIR-0 exhibited higher values (P < 0.05) for DSL, DCL, VSL, VCL, BCF, and WOB compared to AIR-25 and AIR-50. Furthermore, after the thermoresistance test performed at 120 h of storage, total motility, progressive motility and rapid motility were negatively affected (P < 0.05) by the increasing amounts of air within the tubes. There was no effect of air amount within the semen doses on acrosome integrity (P > 0.05).Discussion: The presence of air within tubes affects the quality of extended semen during storage, resulting in increase of pH and subsequent decrease in motility preservation. This pH-rise could be explained by the loss of CO2 from the diluted semen to the air compartment within the tube. At the same time, it is possible to infer that this event influences the intracellular pH of the spermatozoa, increasing sperm metabolism and, consequently, impairing sperm motility. Therefore, we conclude that avoiding air presence within storage containers is recommended in order to ensure the quality of boar semen doses.

scholarly journals Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

2019 ◽  
Vol 71 (2) ◽  
pp. 489-499
Author(s):  
B.A. Pereira ◽  
L.G.P. Rocha ◽  
M.C. Teles ◽  
W.E. Silva ◽  
J.A. Barbosa ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Sperm Motility ◽  
Semen Quality ◽  
Semen Parameters ◽  
Mitochondrial Activity ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Acrosomal Integrity

ABSTRACT A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

Value of semen parameters, with special reference to TNF-α, in predicting the quality of boar semen after short-term storage

2013 ◽  
Vol 61 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Janko Mrkun ◽  
Marjan Kosec ◽  
Petra Zrimšek
Keyword(s):  
Semen Quality ◽  
Semen Parameters ◽  
Short Term ◽  
Progressive Motility ◽  
Tnf Α ◽  
Semen Storage ◽  
Boar Semen ◽  
Α Level ◽  
Term Storage

The aim of this study was to address the question whether changes in boar semen quality after short-term storage could be predicted on the basis of standard semen parameters and TNF-α level determined on the day of semen collection under commercial conditions. Progressive motility showed the highest positive correlation with morphology on day 0 of collection, and progressive motility on day 3 (P < 0.05) showed a negative correlation with acrosome abnormalities (P < 0.05). According to the area under receiver operating characteristics (ROC) curves (AUCs), progressive motility could also be used in predicting semen quality after 3 days of storage (AUC > 0.5; P < 0.05). TNF-α in seminal plasma is the only parameter measured on day 0 to show a significant correlation with the percentage of viable spermatozoa after 3 days of semen storage (r = 0.495, P < 0.05). ROC analysis shows that TNF-α level is helpful in discriminating viability outcome after semen storage (AUC = 0.94, P < 0.001). We can predict with 92.35% certainty that fresh semen samples with more than 150 pg/ml of TNF-α in the seminal plasma will retain more than 85% of viable spermatozoa after 3 days of storage. Thus, TNF-α can contribute to predicting the quality of short-term stored semen.

23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

2020 ◽  
Vol 32 (2) ◽  
pp. 137
Author(s):  
Y. Pirosanto ◽  
M. Valera ◽  
A. Molina ◽  
J. Dorado ◽  
S. Demyda-Peyrás
Keyword(s):  
Sperm Motility ◽  
Inbreeding Depression ◽  
Negative Correlation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Inbreeding Coefficient ◽  
Semen Parameters ◽  
Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P&lt;0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P&lt;0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P&lt;0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P&gt;0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

scholarly journals A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

2016 ◽  
Vol 85 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Marina Anastasia Karageorgiou ◽  
Georgios Tsousis ◽  
Constantin M. Boscos ◽  
Eleni D. Tzika ◽  
Panagiotis D. Tassis ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Medium Term ◽  
Semen Collection ◽  
Chromatin Integrity ◽  
Boar Semen ◽  
Short Time

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

scholarly journals Artificial insemination and cryopreservation of boar semen: current state and problematics

2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Joaquim Moreira da Silva
Keyword(s):  
Artificial Insemination ◽  
Cold Shock ◽  
Current Status ◽  
Freezing And Thawing ◽  
Swine Industry ◽  
Current State ◽  
Boar Semen ◽  
The Many ◽  
Cryopreservation Method

 Commercial artificial insemination with boar semen still prefers the usage of refrigerated semen up to 5 days over frozen-thawed, to date. This is because of the uneconomical properties of frozen-thawed boar semen, such as low motility, viability, fertility rates and the need for higher semen doses, because of the decreased quality after cryopreservation. Since boar semen is highly susceptive to cold shock damage, the invention of a successful cryopreservation method would be greatly beneficial for the swine industry. This review briefly focuses on the many factors that influence the quality of frozen-thawed boar semen, including the different compositions of extenders, comparison of commercial extenders, freezing and thawing methods (temperature and duration). It could be concluded from the present review that optimum freezing/thawing protocol for swine is not standardized, so far being the current status still considered poor-to-fair. 

scholarly journals Influences of bull age and season on sperm motility, sperm concentration, and ejaculate volume of Ongole Grade cattle in Singosari National Artificial Insemination Center

2020 ◽  
Vol 45 (4) ◽  
pp. 261-267
Author(s):  
S. Suyadi ◽  
B. Purwantara ◽  
A. Furqon ◽  
W. A. Septian ◽  
I. Novianti ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Dry Season ◽  
Production Data ◽  
Wet Season

Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.

PSV-9 Effects of Natural Honey Inclusion in Dilution and Freezing Extenders on Frozen-thawed Semen Quality in Boars

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 211-211
Author(s):  
Kayode B Balogun ◽  
Griffin Nicholls ◽  
Olujide Sokunbi ◽  
Kara R Stewart
Keyword(s):  
Semen Quality ◽  
Swine Industry ◽  
Normal Cells ◽  
Progressive Motility ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Boar Semen ◽  
Semen Extender ◽  
Natural Honey

Abstract Improvements in the post-thaw quality of frozen semen could allow increased utilization of this technology in the swine industry. The objective of this study was to investigate the effects of natural honey inclusion in semen extender and freezing media on motility, mobility, and morphology of cryopreserved boar sperm. Ejaculates from 6 terminal cross-bred boars were collected using the gloved-hand technique for 3 weeks and used in a 2 x 3 factorial study design. Following collection, semen samples were incubated overnight in dilution extender with and without natural honey (D0: Androhep Plus; D1: Androhep Plus + 0.25% honey). The following day, the semen samples were cooled to 4 C in LEY cooling extender then frozen in freezing media containing 93% cooling extender + 6% glycerol + 1% Equex-STM Paste (F1), or freezing media with natural honey replacing 50% of the glycerol (F2) or 50% of the Equex-STM paste (F3). Semen samples were frozen using a controlled-rate freezer and stored in liquid nitrogen. Two straws per treatment for each boar were thawed and semen quality assessed. The inclusion of natural honey in dilution extender had no effect on post-thaw motility (P=0.733), progressive motility (P=0.562), or other mobility parameters (0.995≤P≥0.081). However, D1 had a higher percentage of normal acrosomes (P=0.001) and morphologically normal cells (P&lt; 0.001) resulting from lower tail abnormalities compared to D0 (P=0.006). Post-thaw motility (P&lt; 0.001) and progressive motility (P&lt; 0.001) were increased in F3 compared to both F2 and F1. F1 had reduced normal acrosomes (P=0.009) and morphologically normal cells (P&lt; 0.001) resulting from higher tail abnormalities (P&lt; 0.001). In conclusion, the inclusion of natural honey, at 0.25%, in dilution extender helps maintain normal sperm and acrosome morphology, and replacing 50% Equex-STM Paste with honey in freezing extender improves post-thaw sperm motility and progressive motility of frozen-thawed boar semen.

scholarly journals Boar semen quality of the Přeštice black-pied breed (Short Communication)

2011 ◽  
Vol 54 (4) ◽  
pp. 374-380 ◽  
Author(s):  
S. Frydrychová ◽  
A. Lustyková ◽  
J. Lipenský ◽  
M. Rozkot
Keyword(s):  
Dilution Rate ◽  
Sperm Motility ◽  
Short Communication ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Test Period ◽  
Boar Semen ◽  
Natural Mating

Abstract. The objective of this study was to examine semen quality parameters for the Přeštice black-pied breed over a test period of 8 years while considering the potential effects of collection month and boar age. Ninety-nine ejaculates were collected using the gloved-hand technique from healthy and fertile mature boars from selected farms. Ejaculate volumes were relatively low because the boars were accustomed to natural mating. Sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa (MAS), and sperm motility after 24 h of storage in Androhep extender (dilution rate 1:1.5) were assessed. Significant differences were found in sperm concentration and MAS rate in relation to collection months and boar age in the monitored years (P<0.05). A tendency for MAS to increase with monitored years was observed. Significant differences in sperm motility and motility after 24 h of storage were only observed in relation to collection months (P<0.05). Results of this study detected effects due to collection month and boar age on boar semen quality during the monitored years.

scholarly journals Spectrophotometric Analysis of the Resazurin Reduction Test as a Tool for Assessing Canine Semen Quality

2013 ◽  
Vol 57 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Rafał Strzeżek ◽  
Krystyna Filipowicz ◽  
Marta Stańczak ◽  
Władysław Kordan
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Spectrophotometric Analysis ◽  
Normal Morphology ◽  
Additional Tool ◽  
Reduction Test ◽  
Highly Correlated

Abstract The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

scholarly journals Effect of Supplementation of Polyvinylpyrrolidone in Extender on Buffalo Semen Parameters

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Bushra Ismail Khan ◽  
Shamim Akhter ◽  
Sanwal Aslam ◽  
Rabea Ejaz
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Bubalus Bubalis ◽  
Semen Parameters ◽  
Suction Pump ◽  
Bull Semen ◽  
Buffalo Semen ◽  
Live Sperm ◽  
And Control

The current study was planned to evaluate the supplementation of Polyvinylpyrrolidone in extender on cryopreservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentrations of PVP (0.01, 0.05, 0.1mM). The extender without PVP was kept as control. Semen was stored at 4°C for a period of 2 h and kept at 4°C for 4h. Semen was filled in 0.5 ml French straws using suction pump at 4°C, plunged and stored in liquid nitrogen (-196°C). Semen straws were rewarmed at 37°C for 30 seconds and assessed for sperm motility, plasma membrane integrity (PMI), dead sperm percentage and the live sperm percentage. The data on the role of PVP on different parameters of semen quality were analyzed by using ANOVA and RCBD. Higher percentage (P< 0.05) of sperm motility (66.1±7.51 and 59.4±10.72) and PMI (72.9±5.39 and 75.7±6.5) was observed in extenders having 0.05 mM and 0.1mM PVP compared to extenders having 1.5mM PVP and control. The percentage acrosomal integrity was observed greater (P< 0.05) in extended semen containing 0.1mM (68.2±0.50) PVP compared to extenders having 0.01 and control.

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