Changes of Allergenicity and Conformational Structure of Egg Ovomucoid by Gamma Irradiation in the Basic Condition

2002 ◽  
Vol 7 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Kun-Og Kang ◽  
Ju-Woon Lee ◽  
Cheo-Run Jo ◽  
Hong-Sun Yook ◽  
Myung-Woo Byun
Keyword(s):  
Gamma Irradiation ◽  
Conformational Structure

Synthesis, Quantum Chemical Calculations, Molecular Docking and Studying the Effect of High Energetic Gamma Irradiation on Cu(I, II), Zn(II) and Cd(II) Complexes with their Antibacterial Activity.

2021 ◽  
Author(s):  
Hussein Elganzory
Keyword(s):  
Molecular Docking ◽  
Gamma Irradiation ◽  
Density Functional ◽  
Topoisomerase Ii ◽  
Gamma Ray ◽  
Inhibitory Effect ◽  
Molar Conductance ◽  
Basis Set ◽  
Conformational Structure ◽  
Before And After

Abstract New complexes of Cu(I,II), Zn(II) and Cd(II) of thiosemicarbazide ligand 1-(p-(methylanilinocetyl-4-phenyl-thiosemicarbazide)(H2LB) have been prepared and characterized by 1HNMR, Mass spectra, FT-IR, elemental analyses, molar conductance, UV-visible spectra, magnetic susceptibility measurements, thermogravimetric analysis (TGA/DTG) and X-ray diffraction pattern before and after irradiation. The results confirmed that gamma ray enhanced the stability of irradiated compounds as compared to non-irradiated compounds. XRD patterns proved that increasing the crystallinity of the samples and the particles in nano range after gamma irradiation. The obtained data indicated that the Cu(I) and Cd(II) ions coordinated to the ligand through the (C = O), N(2)H and (C = S), the ligand behaves as neutral tridentate. While in complexes Cu(II) and Zn(II)complexes (B2 and B3) the ligand behave as neutral tetradentate and coordination take place via (C = O) and two N(2)H. These studies revealed that, two kinds of stereochemical geometries; Cu(II) and Zn(II) complexes were predicted to be octahedral, Cu(I) and Cd(II)complexes were found to be tetrahedral. The theoretical conformational structure analyses were performed using density functional theory for ligand and complexes at B3LYP functional with 6-31G(++)d,p basis set for ligand and LANL2DZ basis set for complexes. The ligand and its metal complexes have been tested for their inhibitory effect on the growth of bacteria against gram-positive (Streptococcus pyogenes) and gram-negative (Escherichia coli). Results suggested that in case of 1µg/ml and 5µg/ml for Cu(II) and Zn(II) complexes have higher activity than other complexes. The chelation could facilitate the ability to cross the cell membrane of E. coli and can be explained by Tweedy’s chelation theory. Molecular docking investigation proved that; the Zn(II) complex had interesting interactions with active site amino acids of topoisomerase II DNA gyrase enzymes (code: 2XCT).

scholarly journals Synthesis, Biological evaluation, Quantum Chemical Calculations, Molecular Docking and Effect of High Energetic Gamma Irradiation on Cu(I, II), Zn(II) and Cd(II) Complexes.

2021 ◽  
Author(s):  
Hussein Elganzory
Keyword(s):  
Molecular Docking ◽  
Gamma Irradiation ◽  
Topoisomerase Ii ◽  
Gamma Ray ◽  
Inhibitory Effect ◽  
Biological Evaluation ◽  
Basis Set ◽  
Tridentate Ligand ◽  
Conformational Structure ◽  
The Stability

Abstract Novel complexes of Cu(I,II), Zn(II) and Cd(II) of thiosemicarbazide ligand (H2LB) have been prepared and characterized. The results confirmed that gamma ray enhanced the stability of irradiated compounds as compared to non-irradiated . XRD patterns proved that increasing the crystallinity of particles in nano range after gamma irradiation. Data obtained indicated that the Cu(I) and Cd(II) ions coordinated to the ligand through the (C=O), N(2)H and (C=S), (neutral tridentate ligand). While, Cu(II) and Zn(II) complexes the ligand behaves as neutral tetradentate and coordination take place via (C=O) and two N(2)H . These studies revealed that, two kinds of stereochemical geometries; Cu(II) and Zn(II) complexes were predicted to be octahedral, Cu(I) and Cd(II) complexes were found to be tetrahedral. The theoretical conformational structure analyses were performed using DFT at B3LYP functional with 6‐31G(++)d,p basis set for ligand and LANL2DZ basis set for complexes. The inhibitory effect on the growth against gram-positive and gram-negative bacteria of prepared complexes have been tested. Results suggested that 1μg/ml and 5µg/ml for Cu(II) and Zn(II) complexes have higher activity than other complexes. The chelation could facilitate the ability to cross the cell membrane of E. coli. Molecular docking investigation proved that; the Zn(II) complex has interesting interactions with active site amino acids of topoisomerase II DNA gyrase enzymes (code: 2XCT).

The effects of gamma irradiation on the survival and development of Clonorchis sinensis metacercariae

1989 ◽  
Vol 27 (3) ◽  
pp. 187 ◽  
Author(s):  
S H Lee ◽  
Y H Park ◽  
W M Sohn ◽  
S T Hong ◽  
J Y Chai
Keyword(s):  
Gamma Irradiation ◽  
Clonorchis Sinensis ◽  
Survival And Development

Enhanced Stability and Controlled Delivery of MOF Encapsulated Vaccines and Their Immunogenic Response In Vivo

2018 ◽  
Author(s):  
Michael Luzuriaga ◽  
Raymond P. Welch ◽  
Madushani Dharmawardana ◽  
Candace Benjamin ◽  
Shaobo Li ◽  
...  
Keyword(s):  
Viral Vector ◽  
Controlled Delivery ◽  
Conformational Structure ◽  
Spectroscopy Analysis ◽  
Zeolitic Imidazolate Framework ◽  
Structural Conformation ◽  
Depth Study ◽  
Viral Nanoparticle

<div><div><div><p>Vaccines have an innate tendency to lose their structural conformation upon environmental and chemical stressors. A loss in conformation reduces the therapeutic ability to prevent the spread of a pathogen. Herein, we report an in-depth study of zeolitic imidazolate framework-8 (ZIF-8) and its ability to provide protection for a model viral vector against dena- turing conditions. The immunoassay and spectroscopy analysis together demonstrate enhanced thermal and chemical stability to the conformational structure of the encapsulated viral nanoparticle. The long-term biological activity of this virus-ZIF composite was investigated in animal models to further elucidate the integrity of the encapsulated virus, the bio-safety, and immunogenicity of the overall composite. Additionally, histological analysis found no observable tissue damage in the skin or vital organs in mice, following multiple subcutaneous administrations. This study shows that ZIF-based protein composites are strong candidates for improved preservation of proteinaceous drugs, are biocompatible, and capable of controlling the release and adsorption of drugs in vivo.</p></div></div></div>

Thermoluminescence properties of micro and nano structure hydroxyapatite after gamma irradiation

Kerntechnik ◽  
2016 ◽  
Vol 81 (6) ◽  
pp. 651-654 ◽  
Author(s):  
M. Shafaei ◽  
F. Ziaie ◽  
N. Hajiloo
Keyword(s):  
Gamma Irradiation ◽  
Nano Structure

THE EFFECT OF GAMMA STERILIZATION AND ITS EFFECT ON MICROSCOPIC STRUCTURE OF DEEP FROZEN DOG SKULL

2018 ◽  
pp. 37-40
Author(s):  
Phuong Thao Tien Nguyen ◽  
Anh Hung Tran ◽  
Van Tam Le ◽  
Huu Thao Phung ◽  
Phan Quynh Anh Nguyen
Keyword(s):  
Structural Change ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Key Words ◽  
Bone Matrix ◽  
Infected Tissue ◽  
Microscopic Structure ◽  
Bacteriological Examination ◽  
Microscopic Scale

Objective: To evaluate the effect of gamma sterilization and its effect on microscopic structure of deeply frozen dog skull. Subjects and methods: 50 pairs of dog skull fragments have the same size,which were taken symmetrically through the midline of the skull, were divided into two lots. Evident lot consisting of 50 pieces are just deeply frozen, not irradicated. Experimental lot which was composed of 50 pieces were deeply frozen and exposed to 25kGy of gamma radiation. Afterwards, to evaluate the effect of gamma sterilization and compare the alter of bone matrix between lot groups. Results: After gamma irradiation, all dog skull fragments were negative with bacteriological examination. In microscopic scale, no difference was found between the two lots and there was no structural change in the irradiated fragments of all dog skull. Conclusion: Gamma irradiation with 25 kGy: (i) It has the ability to kill bacteria completely in the type of bacteria commonly infected tissue. (ii) It does not alter the structure of dog skull in microstructures. Key words: dog skull, gamma, sterilize

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