scholarly journals The Effect of Sweet Lupine Seed Hulls on the Probiotic Viability of Strained Yogurt

2021 ◽  
pp. 1-5
Author(s):  
Fahad Al-Asmari
Keyword(s):  
Starter Culture ◽  
Bifidobacterium Bifidum ◽  
Lactobacillus Bulgaricus ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Related Factors ◽  
Lupine Seed ◽  
Keywords Probiotics ◽  
Bowel Diseases ◽  
Inflammatory Bowel

Recent advances in gut microbial flora research have shown the health benefits of probiotic bacterial strains on the small and large intestines. These strains, particularly Bifidobacterium, offer health advantages, including obesity, atopic diseases, inflammatory bowel diseases, and intestinal cancers in the human body. However, food components, additives, and processing-related factors can have an impact on probiotic survival. As a result, adding appropriate supplements to increase the viability of probiotics may be necessary for some food products. This study investigated the influence of sweet lupine (Lupineus albus L.) seed hulls (SLSH) on the probiotic viability of strained yogurt. Traditional strained yogurt prepared using Bifidobacterium bifidum and the two starter culture strains Streptococcus thermophiles, and Lactobacillus bulgaricus. The strained yogurt was supplemented with different concentrations of SLSH (1, 2, and 3%). Over 7 to 14 days of cold storage, the total bacterial cells were enumerated. The result showed B. bifidum strains increasing more than double with a slight decrease in the starter culture strains. The overall acceptance of strained yogurt supplemented with 1% SLSH was convenient, compared to 2% and 3% of SLSH. KEYWORDS Probiotics, viability, lupine, B. bifidum, strained yogurt

scholarly journals Kinetic properties of pyruvate ferredoxin oxidoreductase of intestinal sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

2015 ◽  
Vol 64 (2) ◽  
pp. 107-114 ◽  
Author(s):  
IVAN V. KUSHKEVYCH
Keyword(s):  
Enzyme Reaction ◽  
Sulfate Reducing Bacteria ◽  
Kinetic Properties ◽  
Bacterial Strains ◽  
Ferredoxin Oxidoreductase ◽  
Sulfate Reducing ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Pyruvate Ferredoxin Oxidoreductase ◽  
Reducing Bacteria

Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate via pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. have never been well-characterized and have not been yet studied. In this paper we present for the first time the specific activity of pyruvate-ferredoxin oxidoreductase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains. Microbiological, biochemical, biophysical and statistical methods were used in this work. The optimal temperature (+35°C) and pH 8.5 for enzyme reaction were determined. The spectral analysis of the puri- fied pyruvate-ferredoxin oxidoreductase from the cell-free extracts was demonstrated. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period) and maximum velocity of the pyruvate-ferredoxin oxidoreductase reaction (V ) were defined. Michaelis constants (Km) of the enzyme reaction were calculated for both intestinal bacterial strains. The studies of the kinetic enzyme properties in the intestinal sulfate-reducing bacteria strains in detail can be prospects for clarifying the etiological role of these bacteria in the development of inflammatory bowel diseases.

scholarly journals Acetate kinase Activity and Kinetic Properties of the Enzyme in Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 Intestinal Bacterial Strains

2014 ◽  
Vol 8 (1) ◽  
pp. 138-143 ◽  
Author(s):  
Ivan V Kushkevych
Keyword(s):  
Kinase Activity ◽  
Specific Activity ◽  
Enzyme Reaction ◽  
Acetate Kinase ◽  
Kinetic Properties ◽  
Dissimilatory Sulfate Reduction ◽  
Bacterial Strains ◽  
Bowel Diseases ◽  
Michaelis Constants ◽  
Inflammatory Bowel

Activity of acetate kinase in cell-free extracts and individual fractions and the kinetic properties of the enzyme obtained from theDesulfovibrio pigerVib-7 andDesulfomicrobiumsp. Rod-9 intestinal bacterial strains were presented at the first time. The highest activity of the enzyme was measured in the cell-free extracts (1.52 ± 0.163 and 0.46 ± 0.044 U × mg-1 protein forD. pigerVib-7 andDesulfomicrobiumsp. Rod-9, respectively) compared to other fractions. The specific activity of acetate kinase in the extracts of both bacterial strains was determined at different temperature and pH. Analysis of the kinetic properties of the purified acetate kinase was carried out. The acetate kinase activity, initial (instantaneous) reaction rate (V0) and maximum rate of the acetate kinase reaction (Vmax) inD. pigerVib-7 andDesulfomicrobiumsp. Rod-9 intestinal bacterial strains were defined. Michaelis constants (KmAcetyl phosphate and KmADP) of the enzyme reaction (2.54 ± 0.26 and 2.39 ± 0.24 mM forD. pigerVib-7 as well as 2.68 ± 0.25 and 2.47 ± 0.27 mM forDesulfomicrobiumsp. Rod-9, respectively) were calculated. The described results of acetate kinase, an important enzyme in the process of organic compounds oxidation and dissimilatory sulfate reduction would be perspective and useful for clarification of the etiological role of these bacteria in the development of inflammatory bowel diseases in humans and animals.

scholarly journals A Rapid Screening Method of Candidate Probiotics for Inflammatory Bowel Diseases and the Anti-inflammatory Effect of the Selected Strain Bacillus smithii XY1

2021 ◽  
Vol 12 ◽  
Author(s):  
Xuedi Huang ◽  
Fang Ai ◽  
Chen Ji ◽  
Pengcheng Tu ◽  
Yufang Gao ◽  
...  
Keyword(s):  
Screening Method ◽  
Bacillus Coagulans ◽  
Neutrophil Infiltration ◽  
Rapid Screening ◽  
Cell Integrity ◽  
Related Factors ◽  
Bowel Diseases ◽  
Probiotic Intervention ◽  
Inflammatory Bowel ◽  
Bacillus Smithii

Inflammatory bowel disease (IBD) is a chronic intestinal disease associated with the inflammatory gastrointestinal tract and microbiome dysbiosis. Probiotics are a promising intervention, and several probiotics have been reported to positively affect IBD remission and prevention, particularly on ulcerative colitis (UC). However, there is still a limitation in the knowledge of effectiveness and safety of probiotics therapies for IBD. Exploring more potential probiotics helps to find extensive evidence for probiotic intervention. This study established a rapid method for probiotics candidate screening and finally screened out one strain with the best protective effect. Forty strains isolated from four different sources were used for this screening. Hemolysis tests and acute toxic test evaluated strain safety. Zebrafish were first treated with dextran sodium sulfate (DSS) for colitis induction, and every bacteria were individually added to the fish water subsequently. Results showed eight strains could lower the larvae mortality within 3 days under a 0.6% DSS concentration, including Lacticaseibacillus rhamnosus GG, L. rhamnosus NBRC3425, Bacillus smithii DSM4216, B. smithii XY1, Bacillus coagulans NBRC12583, Bacillus coagulans XY2, Lactobacillus parafarraginis XYRR2, and Bacillus licheniformis XYT3. Among eight, B. smithii XY1 was the only strain having the equal ability to alleviate neutrophil infiltration in the larvae intestine with that ability of prednisolone under a 0.5% DSS concentration. Bacillus smithii XY1 restored intestinal epithelial cell integrity after DSS damage, as well as regulated the gene expression inflammation-related factors, indicating its bio-function of inflammatory response alleviation.

scholarly journals The Bowel Microbiota and Inflammatory Bowel Diseases

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Gerald W. Tannock
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Diseases ◽  
Bacterial Species ◽  
Bacterial Cells ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Specific Species ◽  
Bowel Mucosa ◽  
Microbiological Research

The human bowel contains a large and biodiverse bacterial community known as the microbiota or microbiome. It seems likely that the microbiota, fractions of the microbiota, or specific species comprising the microbiota provide the antigenic fuel that drives the chronic immune inflammation of the bowel mucosa that is characteristic of Crohn's disease and ulcerative colitis. At least twenty years of microbiological research have been expended on analysis of the composition of the bowel microbiota of inflammatory bowel disease patients in comparison to that of control subjects. Despite extensive speculations about the aetiological role of dysbiosis in inflammatory bowel diseases, knowledge that can be easily translated into effective remedies for patients has not eventuated. The causes of this failure may be due to poorly defined and executed bacteriological studies, as well as the overwhelming complexity of a biome that contains hundreds of bacterial species and trillions of bacterial cells.

Hepatitis B and C virus prevalence and related factors in patients with inflammatory bowel diseases

2011 ◽  
Vol 35 (12) ◽  
pp. 857 ◽  
Author(s):  
Mustafa Yakut ◽  
Gökhan Kabaçam ◽  
Yusuf Üstün ◽  
Hülya Cetinkaya ◽  
Irfan Soykan
Keyword(s):  
Hepatitis B ◽  
Inflammatory Bowel Diseases ◽  
Related Factors ◽  
Virus Prevalence ◽  
Bowel Diseases ◽  
Inflammatory Bowel

scholarly journals A41 LINKING THE APPENDIX MICROBIOME WITH INFLAMMATORY BOWEL DISEASES

2021 ◽  
Vol 4 (Supplement_1) ◽  
pp. 270-271
Author(s):  
N Arjomand Fard ◽  
H Armstrong ◽  
M W Carroll ◽  
H Q Huynh ◽  
E Wine
Keyword(s):  
Inflammatory Bowel Diseases ◽  
Host Cells ◽  
Intestinal Epithelial ◽  
Related Factors ◽  
Shotgun Metagenomics ◽  
Funding Agencies ◽  
Bowel Diseases ◽  
Recent Method ◽  
Inflammatory Bowel

Abstract Background The appendix has been shown to be associated with the pathogenesis and health outcomes in inflammatory bowel diseases (IBD). Specifically, post appendectomy patients are found to be protective for development of ulcerative colitis (UC); however, mechanisms of appendix involvement remain unclear. Aims Our aim is to examine the microbes associated with the appendix of IBD patients by identifying changes in microbe abundance and interactions with the host in patient cecum luminal washes, collected from close to the neck of the appendix during colonoscopy. We hypothesize that microbes originating in the appendix of IBD patients, through interactions with host-cells in a disrupted microenvironment in the appendix, could contribute to the pathogenesis of UC. Methods Shotgun metagenomics was performed on cecum luminal washes of IBD patients and non-IBD controls. Guided by the metagenomic results, we performed gentamicin protection assays to determine virulence of microbes of interest using Caco2 intestinal epithelial cells. Co-culturing them with human host cells in vitro will identify relevant disease-related factors secreted by microbes and/or host cells using disease models and multiomic approaches. Results Shotgun metagenomics results showed that among numerous microbes, several bacterial taxa demonstrated differences in abundance between IBD and non-IBD patients: Flavonifractor, Bacteroide fragilis, and Alistipes represented 8%, 10%, and 21% abundance respectively in non-IBD patients, while in IBD patients they were present below 0.1%. In contrast, Bacteroide vulgatus and Escherichia coli were about 9% and 69% respectively, in IBD patients, whilst they were present at 1.7% and 1.2% in non-IBD patients, respectively. Following our recent method for validating pathobionts (Armstrong, 2019), we used the gentamicin protection assays to assess the ability of these bacteria to invade Caco2 cells, demonstrating a correlation between invasive potential of these microbes and cecal abundance. Mechanistic experiments, aimed at identifying factors impacting invasion, are in progress. Conclusions These results provide preliminary, but promising findings suggesting mechanisms by which microbiota possibly originating in the appendix may show altered virulence, which may be related to changes in the appendix microenvironment in IBD. With plans in place to increase our patient cohort we will validate these findings. Identifying and profiling these microbes in IBD patients can help improve the understanding of mechanisms underlying microenvironment changes within the appendix and the gut, which could shed light on the role of the appendix in IBD pathogenesis and clarify how microbes drive inflammation in IBD. Funding Agencies CIHR

An Overview of the Therapeutic Aspect of Living Drugs Probiotics

2019 ◽  
pp. 1-34
Author(s):  
Vandana Bharat Patravale ◽  
Amita Keertimaan Joshi
Keyword(s):  
Human Microbiome ◽  
Positive Influence ◽  
Vital Role ◽  
Bacterial Cells ◽  
Therapeutic Aspect ◽  
Activity Density ◽  
Bowel Diseases ◽  
Research Findings ◽  
Inflammatory Bowel ◽  
Micro Organisms

The human body harbors ten times more bacterial cells than human cells. These bacterial cells form the human microbiome that plays a vital role in human health. An imbalance in the gut microbiome (i.e., dysbiosis) can result in various pathological conditions. This dysbiosis can be refurbished with the supplement of “probiotics.” Probiotics have thus been defined as non-pathogenic micro-organisms that, when ingested, exert a positive influence on host health or physiology. The most commonly used probiotic bacteria comes from two genera: Lactobacillus and Bifidobacterium. Various research findings have proposed a correlation between the alteration of microbiota (composition/activity/density) with disorders like infectious diarrhea, inflammatory bowel diseases, obesity, to name a few. The ultimate effect of administration of probiotics on health or physiology is either direct or indirect. This chapter summarizes the concept of probiotics, their therapeutic aspect along with possible mechanism of action.

Sign in / Sign up

Export Citation Format

Share Document