A Rapid Screening Method of Candidate Probiotics for Inflammatory Bowel Diseases and the Anti-inflammatory Effect of the Selected Strain Bacillus smithii XY1

Inflammatory bowel disease (IBD) is a chronic intestinal disease associated with the inflammatory gastrointestinal tract and microbiome dysbiosis. Probiotics are a promising intervention, and several probiotics have been reported to positively affect IBD remission and prevention, particularly on ulcerative colitis (UC). However, there is still a limitation in the knowledge of effectiveness and safety of probiotics therapies for IBD. Exploring more potential probiotics helps to find extensive evidence for probiotic intervention. This study established a rapid method for probiotics candidate screening and finally screened out one strain with the best protective effect. Forty strains isolated from four different sources were used for this screening. Hemolysis tests and acute toxic test evaluated strain safety. Zebrafish were first treated with dextran sodium sulfate (DSS) for colitis induction, and every bacteria were individually added to the fish water subsequently. Results showed eight strains could lower the larvae mortality within 3 days under a 0.6% DSS concentration, including Lacticaseibacillus rhamnosus GG, L. rhamnosus NBRC3425, Bacillus smithii DSM4216, B. smithii XY1, Bacillus coagulans NBRC12583, Bacillus coagulans XY2, Lactobacillus parafarraginis XYRR2, and Bacillus licheniformis XYT3. Among eight, B. smithii XY1 was the only strain having the equal ability to alleviate neutrophil infiltration in the larvae intestine with that ability of prednisolone under a 0.5% DSS concentration. Bacillus smithii XY1 restored intestinal epithelial cell integrity after DSS damage, as well as regulated the gene expression inflammation-related factors, indicating its bio-function of inflammatory response alleviation.

Download Full-text

Synbiotic supplementation with prebiotic green banana resistant starch and probiotic Bacillus coagulans spores ameliorates gut inflammation in mouse model of inflammatory bowel diseases

European Journal of Nutrition ◽

10.1007/s00394-020-02200-9 ◽

2020 ◽

Vol 59 (8) ◽

pp. 3669-3689 ◽

Cited By ~ 8

Author(s):

Tanvi Shinde ◽

Agampodi Promoda Perera ◽

Ravichandra Vemuri ◽

Shakuntla V. Gondalia ◽

David J. Beale ◽

...

Keyword(s):

Inflammatory Bowel Diseases ◽

Resistant Starch ◽

Animal Health ◽

Bacillus Coagulans ◽

Chow Diet ◽

Inflammatory Status ◽

Bowel Diseases ◽

Inflammatory Bowel ◽

Synbiotic Supplementation ◽

Green Banana

Abstract Purpose The research goal is to develop dietary strategies to help address the growing incidence of inflammatory bowel diseases (IBD). This study has investigated the effectiveness of green banana resistant starch (GBRS) and probiotic Bacillus coagulans MTCC5856 spores for the amelioration of dextran-sulfate sodium (DSS)-induced colitis in mice. Methods Eight-week-old C57BL/6 mice were fed standard rodent chow diet supplemented with either B. coagulans, GBRS or its synbiotic combination. After 7 days supplementation, colitis was induced by adding 2% DSS in drinking water for 7 days while continuing the supplemented diets. Animal health was monitored and after 14 days all animals were sacrificed to measure the biochemical and histochemical changes associated with each supplement type. Results The disease activity index and histological damage score for DSS-control mice (6.1, 17.1, respectively) were significantly higher (p < 0.0001) than the healthy mice. Synbiotic supplementation alleviated these markers (− 67%, − 94% respectively) more adequately than B. coagulans (− 52%, − 58% respectively) or GBRS (− 57%, − 26%, respectively) alone. Compared to DSS-control synbiotic supplementation significantly (p < 0.0001) maintained expressions of tight junction proteins. Moreover, synbiotic effects accounted for ~ 40% suppression of IL-1β and ~ 29% increase in IL-10 levels in serum while also reducing C-reactive protein (− 37%) compared to that of the DSS-control. While, B. coagulans alone could not induce additional levels of short-chain fatty acid (SCFA) production beyond the caecum, the synbiotic combination with GBRS resulted in substantial increased SCFA levels across the whole length of the colon. Conclusion The synbiotic supplementation with B. coagulans and GBRS ameliorated the overall inflammatory status of the experimental IBD model via synergistic functioning. This supports researching its application in mitigating inflammation in human IBD.

Download Full-text

The Effect of Sweet Lupine Seed Hulls on the Probiotic Viability of Strained Yogurt

Basic and Applied Sciences - Scientific Journal of King Faisal University ◽

10.37575/b/agr/210061 ◽

2021 ◽

pp. 1-5

Author(s):

Fahad Al-Asmari

Keyword(s):

Starter Culture ◽

Bifidobacterium Bifidum ◽

Lactobacillus Bulgaricus ◽

Bacterial Cells ◽

Bacterial Strains ◽

Related Factors ◽

Lupine Seed ◽

Keywords Probiotics ◽

Bowel Diseases ◽

Inflammatory Bowel

Recent advances in gut microbial flora research have shown the health benefits of probiotic bacterial strains on the small and large intestines. These strains, particularly Bifidobacterium, offer health advantages, including obesity, atopic diseases, inflammatory bowel diseases, and intestinal cancers in the human body. However, food components, additives, and processing-related factors can have an impact on probiotic survival. As a result, adding appropriate supplements to increase the viability of probiotics may be necessary for some food products. This study investigated the influence of sweet lupine (Lupineus albus L.) seed hulls (SLSH) on the probiotic viability of strained yogurt. Traditional strained yogurt prepared using Bifidobacterium bifidum and the two starter culture strains Streptococcus thermophiles, and Lactobacillus bulgaricus. The strained yogurt was supplemented with different concentrations of SLSH (1, 2, and 3%). Over 7 to 14 days of cold storage, the total bacterial cells were enumerated. The result showed B. bifidum strains increasing more than double with a slight decrease in the starter culture strains. The overall acceptance of strained yogurt supplemented with 1% SLSH was convenient, compared to 2% and 3% of SLSH. KEYWORDS Probiotics, viability, lupine, B. bifidum, strained yogurt

Download Full-text

Hepatitis B and C virus prevalence and related factors in patients with inflammatory bowel diseases

Clinics and Research in Hepatology and Gastroenterology ◽

10.1016/j.clinre.2011.08.005 ◽

2011 ◽

Vol 35 (12) ◽

pp. 857 ◽

Cited By ~ 1

Author(s):

Mustafa Yakut ◽

Gökhan Kabaçam ◽

Yusuf Üstün ◽

Hülya Cetinkaya ◽

Irfan Soykan

Keyword(s):

Hepatitis B ◽

Inflammatory Bowel Diseases ◽

Related Factors ◽

Virus Prevalence ◽

Bowel Diseases ◽

Inflammatory Bowel

Download Full-text

A41 LINKING THE APPENDIX MICROBIOME WITH INFLAMMATORY BOWEL DISEASES

Journal of the Canadian Association of Gastroenterology ◽

10.1093/jcag/gwab002.039 ◽

2021 ◽

Vol 4 (Supplement_1) ◽

pp. 270-271

Author(s):

N Arjomand Fard ◽

H Armstrong ◽

M W Carroll ◽

H Q Huynh ◽

E Wine

Keyword(s):

Inflammatory Bowel Diseases ◽

Host Cells ◽

Intestinal Epithelial ◽

Related Factors ◽

Shotgun Metagenomics ◽

Funding Agencies ◽

Bowel Diseases ◽

Recent Method ◽

Inflammatory Bowel

Abstract Background The appendix has been shown to be associated with the pathogenesis and health outcomes in inflammatory bowel diseases (IBD). Specifically, post appendectomy patients are found to be protective for development of ulcerative colitis (UC); however, mechanisms of appendix involvement remain unclear. Aims Our aim is to examine the microbes associated with the appendix of IBD patients by identifying changes in microbe abundance and interactions with the host in patient cecum luminal washes, collected from close to the neck of the appendix during colonoscopy. We hypothesize that microbes originating in the appendix of IBD patients, through interactions with host-cells in a disrupted microenvironment in the appendix, could contribute to the pathogenesis of UC. Methods Shotgun metagenomics was performed on cecum luminal washes of IBD patients and non-IBD controls. Guided by the metagenomic results, we performed gentamicin protection assays to determine virulence of microbes of interest using Caco2 intestinal epithelial cells. Co-culturing them with human host cells in vitro will identify relevant disease-related factors secreted by microbes and/or host cells using disease models and multiomic approaches. Results Shotgun metagenomics results showed that among numerous microbes, several bacterial taxa demonstrated differences in abundance between IBD and non-IBD patients: Flavonifractor, Bacteroide fragilis, and Alistipes represented 8%, 10%, and 21% abundance respectively in non-IBD patients, while in IBD patients they were present below 0.1%. In contrast, Bacteroide vulgatus and Escherichia coli were about 9% and 69% respectively, in IBD patients, whilst they were present at 1.7% and 1.2% in non-IBD patients, respectively. Following our recent method for validating pathobionts (Armstrong, 2019), we used the gentamicin protection assays to assess the ability of these bacteria to invade Caco2 cells, demonstrating a correlation between invasive potential of these microbes and cecal abundance. Mechanistic experiments, aimed at identifying factors impacting invasion, are in progress. Conclusions These results provide preliminary, but promising findings suggesting mechanisms by which microbiota possibly originating in the appendix may show altered virulence, which may be related to changes in the appendix microenvironment in IBD. With plans in place to increase our patient cohort we will validate these findings. Identifying and profiling these microbes in IBD patients can help improve the understanding of mechanisms underlying microenvironment changes within the appendix and the gut, which could shed light on the role of the appendix in IBD pathogenesis and clarify how microbes drive inflammation in IBD. Funding Agencies CIHR

Download Full-text