Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin–carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces strongly dominate over the non-specific ones. However, obtaining quantitative results requires the complex and tedious calibration of a torsional force. Here, a new and relatively simple method for the calibration of the torsional force is presented. The proposed calibration method is validated through the measurement of the interaction forces between human fibronectin and its monoclonal antibody. The results obtained using LFM and AFM-based classical force spectroscopies showed similar unbinding forces recorded at similar loading rates. Our studies verify that the proposed lateral force calibration method can be applied to study single molecule interactions.

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Nano and Micro Mechanical Measurement of Interaction Forces Between Solid Surfaces

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.326-328.1 ◽

2006 ◽

Vol 326-328 ◽

pp. 1-4

Author(s):

Kyung Suk Kim

Keyword(s):

Scale Effects ◽

Measurement Techniques ◽

Calibration Method ◽

Lateral Force ◽

Solid Surfaces ◽

Lateral Interaction ◽

Force Measurements ◽

Interaction Forces ◽

Size Scale ◽

Force Calibration

Two different types of experimental methods have beeen developed for measuring lateral interaction forces between two solid surfaces for nano- and micro-meter scale contacts. One is the type of direct measurement methods which typically utilize AFM instrumentations. In the direct lateral force measurements some size-scale effects are commonly observed due to the effects of adhesion and surface roughness. A recent development of a fine AFM lateral force calibration method, a diamagnetic lateral force calibrator, has made it possible to study such size-scale effects systematically. The other type is the field projection method which requires a high resolution measurement of a deformation field near the edge of a contact. For such measurements a comprehensive map of deformation measurement techniques is introduced in a domain of spatial and strain resolutions. This technique provides a way of assessing the non-uniform distribution of the surface interaction forces for nano and micro-meter scale contacts.

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Force calibration in lateral force microscopy: a review of the experimental methods

Journal of Physics D Applied Physics ◽

10.1088/0022-3727/43/6/063001 ◽

2010 ◽

Vol 43 (6) ◽

pp. 063001 ◽

Cited By ~ 62

Author(s):

Martin Munz

Keyword(s):

Lateral Force ◽

Experimental Methods ◽

Lateral Force Microscopy ◽

Force Microscopy ◽

Force Calibration

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Force Calibration in Lateral Force Microscopy

Journal of Colloid and Interface Science ◽

10.1006/jcis.2000.6840 ◽

2000 ◽

Vol 227 (1) ◽

pp. 55-65 ◽

Cited By ~ 73

Author(s):

Robert G. Cain ◽

Simon Biggs ◽

Neil W. Page

Keyword(s):

Lateral Force ◽

Lateral Force Microscopy ◽

Force Microscopy ◽

Force Calibration

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Lateral force calibration in atomic force microscopy: A new lateral force calibration method and general guidelines for optimization

Review of Scientific Instruments ◽

10.1063/1.2198768 ◽

2006 ◽

Vol 77 (5) ◽

pp. 053701 ◽

Cited By ~ 140

Author(s):

Rachel J. Cannara ◽

Michael Eglin ◽

Robert W. Carpick

Keyword(s):

Atomic Force Microscopy ◽

Calibration Method ◽

Lateral Force ◽

Force Microscopy ◽

Atomic Force ◽

Force Calibration

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Sliding Friction by Atomic Force Microscopy

MRS Proceedings ◽

10.1557/proc-543-51 ◽

1998 ◽

Vol 543 ◽

Author(s):

V. Pasquier ◽

J. M. Drake

Keyword(s):

Atomic Force Microscopy ◽

Sliding Friction ◽

Lateral Force ◽

Lateral Force Microscopy ◽

Force Microscopy ◽

Load Dependence ◽

Silica Surfaces ◽

Atomic Force ◽

Friction Measurements ◽

Force Calibration

AbstractLateral Force Microscopy offers the possibility of exploring tribological properties of interfaces atthe nanoscale. Our research focused on some crucial conditions that must be fuffilled to obtainquantitative and reliable LFM friction measurements. We have characterized the mechanical andvibrational properties of the cantilever. Precise force calibration were made based on ourknowledge of the intrinsic coupling modes of the cantilever. We report measurements of the slidingfriction between two silica surfaces. The load dependence of the friction force was analyzedassuming different models for the contact, from Hertzian to Amontons law.

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