scholarly journals Association Examined of Viscoelastic Properties with the Invasion of Ovarian Cancer Cells by Atomic Force Microscopy

2019 ◽  
Author(s):  
Mengdan Chen ◽  
Jinshu Zeng ◽  
Weiwei Ruan ◽  
Zhenghong Zhang ◽  
Yuhua Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Atomic Force Microscopy ◽  
Single Cell ◽  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Ovarian Cancer Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Force Microscopy ◽  
Atomic Force

Cellular mechanical properties could serve as a prominent indicator for disease progression and early cancer diagnosis. This study utilized atomic force microscopy (AFM) to measure the viscoelastic properties and then examined their association with the invasion of ovarian cancer at living single cell level. The results demonstrated the elasticity and viscosity of ovarian cancer cell OVCAR-3 and HO-8910 significantly decreased than those of HOSEpiC, the ovarian cancer control cell. Further examination found the dramatic increase of migration/invasion and the obvious decease of microfilament density in OVCAR-3 and HO-8910 cells compared with those of HOSEpiC cells. And there was a significant relationship between viscoelastic and biological properties among these cells. In addition, the elasticity was significantly increased in OVCAR-3 and HO-8910 cells after the treatment of anticancer compound echinomycin (Ech), while no obvious change was found in HOSEpiC cells after Ech treatment. Interestingly, Ech seemed no effects on the viscosity of these cells. Furthermore, Ech significantly inhibited the migration/invasion and significantly increased the microfilament density in OVCAR-3 and HO-8910 cells compared with those of HOSEpiC cells, which was significantly related with the elasticity among these cells. Notably, an increase of elasticity and a decrease of invasion were found in OVCAR-3 and HO-8910 cells with Ech treatment. Together, this study clearly demonstrated the association of viscoelastic properties with the invasion of ovarian cancer cells and shed a light on the biomechanical changes for early diagnosis of tumor transformation and progression at single cell level.

scholarly journals Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

2020 ◽  
Vol 11 ◽  
pp. 568-582 ◽  
Author(s):  
Mengdan Chen ◽  
Jinshu Zeng ◽  
Weiwei Ruan ◽  
Zhenghong Zhang ◽  
Yuhua Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Single Cells ◽  
Biological Properties ◽  
Ovarian Cancer Cells ◽  
Force Microscopy ◽  
Early Cancer Diagnosis ◽  
Atomic Force

The mechanical properties of cells could serve as an indicator for disease progression and early cancer diagnosis. This study utilized atomic force microscopy (AFM) to measure the viscoelastic properties of ovarian cancer cells and then examined the association with the invasion of ovarian cancer at the level of living single cells. Elasticity and viscosity of the ovarian cancer cells OVCAR-3 and HO-8910 are significantly lower than those of the human ovarian surface epithelial cell (HOSEpiC) control. Further examination found a dramatic increase of migration/invasion and an obvious decease of microfilament density in OVCAR-3 and HO-8910 cells. Also, there was a significant relationship between viscoelastic and biological properties among these cells. In addition, the elasticity was significantly increased in OVCAR-3 and HO-8910 cells after the treatment with the anticancer compound echinomycin (Ech), while no obvious change was found in HOSEpiC cells after Ech treatment. Interestingly, Ech seemed to have no effect on the viscosity of the cells. Ech significantly inhibited the migration/invasion and significantly increased the microfilament density in OVCAR-3 and HO-8910 cells, which was significantly related with the elasticity of the cells. An increase of elasticity and a decrease of invasion were found in OVCAR-3 and HO-8910 cells after Ech treatment. Together, this study clearly demonstrated the association of viscoelastic properties with the invasion of ovarian cancer cells and shed a light on the biomechanical changes for early diagnosis of tumor transformation and progression at single-cell level.

scholarly journals Direct Detection of Cellular Adaptation to Local Cyclic Stretching at the Single Cell Level by Atomic Force Microscopy

2011 ◽  
Vol 100 (3) ◽  
pp. 564-572 ◽  
Author(s):  
Takahiro Watanabe-Nakayama ◽  
Shin-ichi Machida ◽  
Ichiro Harada ◽  
Hiroshi Sekiguchi ◽  
Rehana Afrin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Direct Detection ◽  
Single Cell Level ◽  
Cell Level ◽  
Cellular Adaptation ◽  
Force Microscopy ◽  
Cyclic Stretching ◽  
Atomic Force

Viscoelastic properties of doxorubicin-treated HT-29 cancer cells by atomic force microscopy: the fractional Zener model as an optimal viscoelastic model for cells

2019 ◽  
Vol 19 (3) ◽  
pp. 801-813 ◽  
Author(s):  
Maricela Rodríguez-Nieto ◽  
Priscila Mendoza-Flores ◽  
David García-Ortiz ◽  
Luis M. Montes-de-Oca ◽  
Marco Mendoza-Villa ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Viscoelastic Model ◽  
Zener Model ◽  
Force Microscopy ◽  
Atomic Force ◽  
Fractional Zener Model ◽  
Ht 29

scholarly journals FluidFM for single-cell biophysics

Nano Research ◽  
2021 ◽  
Author(s):  
Mi Li ◽  
Lianqing Liu ◽  
Tomaso Zambelli
Keyword(s):  
Single Cell ◽  
Three Dimensional ◽  
Cell Biophysics ◽  
Single Cell Level ◽  
Cell Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Underlying Mechanisms ◽  
Aqueous Conditions ◽  
Cell Force

AbstractFluidic force microscopy (FluidFM), which combines atomic force microscopy (AFM) with microchanneled cantilevers connected to a pressure controller, is a technique allowing the realization of force-sensitive nanopipette under aqueous conditions. FluidFM has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements of biological specimens at the micro-/nanoscale. Over the past decade, FluidFM has shown its potential in biophysical assays particularly in the investigations at single-cell level, offering novel possibilities for discovering the underlying mechanisms guiding life activities. Here, we review the utilization of FluidFM to address biomechanical and biophysical issues in the life sciences. Firstly, the fundamentals of FluidFM are represented. Subsequently, the applications of FluidFM for biophysics at single-cell level are surveyed from several facets, including single-cell manipulations, single-cell force spectroscopy, and single-cell electrophysiology. Finally, the challenges and perspectives for future progressions are provided.

Study of NSCLC cell migration promoted by NSCLC-Derived Extracellular Vesicle using atomic force microscopy

2021 ◽  
Author(s):  
Shuwei Wang ◽  
Jiajia Wang ◽  
Tuoyu Ju ◽  
Kaige Qu ◽  
Fan Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Migration ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Extracellular Vesicle ◽  
Cancer Microenvironment ◽  
Biological Processes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cellular Components

Extracellular Vesicles (EVs) secreted by cancer cells have a key role in the cancer microenvironment and progression. Previous studies have mainly focused on molecular functions, cellular components and biological processes...

Sign in / Sign up

Export Citation Format

Share Document