scholarly journals FluidFM for single-cell biophysics

Nano Research ◽  
2021 ◽  
Author(s):  
Mi Li ◽  
Lianqing Liu ◽  
Tomaso Zambelli
Keyword(s):  
Single Cell ◽  
Three Dimensional ◽  
Cell Biophysics ◽  
Single Cell Level ◽  
Cell Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Underlying Mechanisms ◽  
Aqueous Conditions ◽  
Cell Force

AbstractFluidic force microscopy (FluidFM), which combines atomic force microscopy (AFM) with microchanneled cantilevers connected to a pressure controller, is a technique allowing the realization of force-sensitive nanopipette under aqueous conditions. FluidFM has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements of biological specimens at the micro-/nanoscale. Over the past decade, FluidFM has shown its potential in biophysical assays particularly in the investigations at single-cell level, offering novel possibilities for discovering the underlying mechanisms guiding life activities. Here, we review the utilization of FluidFM to address biomechanical and biophysical issues in the life sciences. Firstly, the fundamentals of FluidFM are represented. Subsequently, the applications of FluidFM for biophysics at single-cell level are surveyed from several facets, including single-cell manipulations, single-cell force spectroscopy, and single-cell electrophysiology. Finally, the challenges and perspectives for future progressions are provided.

scholarly journals Direct Detection of Cellular Adaptation to Local Cyclic Stretching at the Single Cell Level by Atomic Force Microscopy

2011 ◽  
Vol 100 (3) ◽  
pp. 564-572 ◽  
Author(s):  
Takahiro Watanabe-Nakayama ◽  
Shin-ichi Machida ◽  
Ichiro Harada ◽  
Hiroshi Sekiguchi ◽  
Rehana Afrin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Cell ◽  
Direct Detection ◽  
Single Cell Level ◽  
Cell Level ◽  
Cellular Adaptation ◽  
Force Microscopy ◽  
Cyclic Stretching ◽  
Atomic Force

scholarly journals Association Examined of Viscoelastic Properties with the Invasion of Ovarian Cancer Cells by Atomic Force Microscopy

2019 ◽  
Author(s):  
Mengdan Chen ◽  
Jinshu Zeng ◽  
Weiwei Ruan ◽  
Zhenghong Zhang ◽  
Yuhua Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Atomic Force Microscopy ◽  
Single Cell ◽  
Cancer Cells ◽  
Viscoelastic Properties ◽  
Ovarian Cancer Cells ◽  
Single Cell Level ◽  
Cell Level ◽  
Force Microscopy ◽  
Atomic Force

Cellular mechanical properties could serve as a prominent indicator for disease progression and early cancer diagnosis. This study utilized atomic force microscopy (AFM) to measure the viscoelastic properties and then examined their association with the invasion of ovarian cancer at living single cell level. The results demonstrated the elasticity and viscosity of ovarian cancer cell OVCAR-3 and HO-8910 significantly decreased than those of HOSEpiC, the ovarian cancer control cell. Further examination found the dramatic increase of migration/invasion and the obvious decease of microfilament density in OVCAR-3 and HO-8910 cells compared with those of HOSEpiC cells. And there was a significant relationship between viscoelastic and biological properties among these cells. In addition, the elasticity was significantly increased in OVCAR-3 and HO-8910 cells after the treatment of anticancer compound echinomycin (Ech), while no obvious change was found in HOSEpiC cells after Ech treatment. Interestingly, Ech seemed no effects on the viscosity of these cells. Furthermore, Ech significantly inhibited the migration/invasion and significantly increased the microfilament density in OVCAR-3 and HO-8910 cells compared with those of HOSEpiC cells, which was significantly related with the elasticity among these cells. Notably, an increase of elasticity and a decrease of invasion were found in OVCAR-3 and HO-8910 cells with Ech treatment. Together, this study clearly demonstrated the association of viscoelastic properties with the invasion of ovarian cancer cells and shed a light on the biomechanical changes for early diagnosis of tumor transformation and progression at single cell level.

scholarly journals In situelectro-sequencing in three-dimensional tissues

2021 ◽  
Author(s):  
Qiang Li ◽  
Zuwan Lin ◽  
Ren Liu ◽  
Xin Tang ◽  
Jiahao Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Three Dimensional ◽  
Cell Types ◽  
Developmental Trajectory ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Gene Expression ◽  
Cell Gene

AbstractPairwise mapping of single-cell gene expression and electrophysiology in intact three-dimensional (3D) tissues is crucial for studying electrogenic organs (e.g., brain and heart)1–5. Here, we introducein situelectro-sequencing (electro-seq), combining soft bioelectronics within situRNA sequencing to stably map millisecond-timescale cellular electrophysiology and simultaneously profile a large number of genes at single-cell level across 3D tissues. We appliedin situelectro-seq to 3D human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) patches, precisely registering the CM gene expression with electrophysiology at single-cell level, enabling multimodalin situanalysis. Such multimodal data integration substantially improved the dissection of cell types and the reconstruction of developmental trajectory from spatially heterogeneous tissues. Using machine learning (ML)-based cross-modal analysis,in situelectro-seq identified the gene-to-electrophysiology relationship over the time course of cardiac maturation. Further leveraging such a relationship to train a coupled autoencoder, we demonstrated the prediction of single-cell gene expression profile evolution using long-term electrical measurement from the same cardiac patch or 3D millimeter-scale cardiac organoids. As exemplified by cardiac tissue maturation,in situelectro-seq will be broadly applicable to create spatiotemporal multimodal maps and predictive models in electrogenic organs, allowing discovery of cell types and gene programs responsible for electrophysiological function and dysfunction.

scholarly journals Heterogeneity in the Rat Brain Vasculature Revealed by Quantitative Confocal Analysis of Endothelial Barrier Antigen and P-Glycoprotein Expression

2011 ◽  
Vol 32 (1) ◽  
pp. 81-92 ◽  
Author(s):  
Bruno Saubaméa ◽  
Véronique Cochois-Guégan ◽  
Salvatore Cisternino ◽  
Jean-Michel Scherrmann
Keyword(s):  
Single Cell ◽  
Rat Brain ◽  
Confocal Imaging ◽  
Endothelial Barrier ◽  
Single Cell Level ◽  
Cell Level ◽  
Brain Vasculature ◽  
P Glycoprotein ◽  
Underlying Mechanisms ◽  
Endothelial Barrier Antigen

While phenotypic endothelial heterogeneity is well documented in peripheral organs, it is only now being explored in the brain. We used confocal imaging of thick sections of rat brain to qualitatively and quantitatively examine the expression of two key markers of the blood—brain barrier (BBB) in the rat, P-glycoprotein (P-gp), and endothelial barrier antigen (EBA). We found that these markers were not uniformly distributed throughout the whole vasculature of the cortex and hippocampus. P-glycoprotein displayed a gradient of expression from an almost undetectable level in large penetrating arterioles to a high and uniform level in capillaries and venules. While EBA was lacking in all cerebral arterioles, regardless of their size, its expression varied greatly among endothelial cells in capillaries and venules, yielding a striking mosaic pattern. A detailed quantitative analysis of the distribution of these markers at the single cell level in capillaries is provided. These results challenge the view of a uniform BBB and suggest that regulatory mechanisms might differentially modulate BBB features not only among arterioles/capillaries/venules but also at the single cell level within the capillaries. Hypotheses are made regarding the underlying mechanisms and physiopathological consequences of this heterogeneity.

scholarly journals Single cell profiling of surface carbohydrates on Bacillus cereus

2015 ◽  
Vol 12 (103) ◽  
pp. 20141109 ◽  
Author(s):  
Congzhou Wang ◽  
Christopher J. Ehrhardt ◽  
Vamsi K. Yadavalli
Keyword(s):  
Single Cell ◽  
Biochemical Properties ◽  
Single Cell Level ◽  
Cell Level ◽  
Bacterial Surface ◽  
Force Microscopy ◽  
Force Mapping ◽  
Single Cell Profiling ◽  
Entire Cell ◽  
Surface Carbohydrates

Cell surface carbohydrates are important to various bacterial activities and functions. It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved. Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N -acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell. Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown. These results demonstrate AFM-based ‘recognition force mapping’ as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.

scholarly journals Quantifying the Effect of Anti-cancer Compound (Piperlongumine) on Cancer Cells Using Single-Cell Force Spectroscopy

2021 ◽  
Author(s):  
Nayara Sousa de Alcântara-Contessoto ◽  
Marinônio Lopes Cornélio ◽  
Ching-Hwa Kiang
Keyword(s):  
Mechanical Properties ◽  
Single Cell ◽  
Hela Cells ◽  
Cell Manipulation ◽  
Tumor Growth Inhibition ◽  
Force Microscopy ◽  
Therapeutic Drugs ◽  
Atomic Force ◽  
Anti Cancer ◽  
Cell Force

AbstractNatural compounds have shown a great potential in anti-cancer research by tumor growth inhibition and anti-metastatic properties. Piperlongumine (PL) is a natural compound derived from pepper species that has been demonstrated to have anti-cancer effect on HeLa cells. Here we focus on understanding the mechanical properties of HeLa cells under PL treatment, using Atomic Force Microscopy (AFM) based single-cell manipulation technique. We used AFM to pull single HeLa cells and acquired the force-distance curves that presented stepwise patterns. We analyzed the step force (SF) and observed that cells treated with PL exhibit higher force compared to control cells. This SF increase was also observed in experiments performed on substrates of different stiffness. Therefore, analyzing SF, it is possible to investigate the effect of PL on the mechanical properties of the HeLa cells. The understanding of the PL action on HeLa cells’ mechanical properties may help in the development of effective therapeutic drugs against cancers.

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