THE USE OF BIOTECHNOLOGY IN THE PREVENTION OF FAILURE IN THE IMPOSITION OF SMALL INTESTINAL ANASTOMOSES IN THE EXPERIMENT

2020 ◽  
pp. 87-91
Author(s):  
M. Tymchenko ◽  
Yu. Ivanova ◽  
V. Padalko ◽  
H. Sheremet ◽  
O. Kozlova ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Intestinal Mucosa ◽  
Intestinal Anastomosis ◽  
Experimental Studies ◽  
Operating Conditions ◽  
Laboratory Animals ◽  
Control Group ◽  
Intestinal Anastomoses ◽  
Series Of Experiments ◽  
Immunohistochemical Studies

Summary. The aimof the study is to develop a method of sealing the sutured of the small intestine anastomosis in conditions of high risk anastomosis leakege by stopping the inflammation’ cascade in experiment. Materials and methods.The work was experimental in nature, was performed in the SI «ZIGUS NAMSU» in collaboration with the Scientific Research Institute of Biology V. N. Karazin KhNU in the period from 2018 to 2019. To increase the tightness of the intestinal anastomoses applied under conditions of laparatomy, experimental studies were conducted on laboratory animals. The studies were carried out after the permission of the ethics committee SI «ZIGUS NAMSU» in compliance with all ethical requirements for the humane treatment of animals.Surgical operations in two series of experiments were performed under operating conditions under general anesthesia on 10 white sexually mature linear rats – Wistar males, 12-18 months old, weighing about 250 grams. All morphological material (the first and second series of experiments) was marked and fixed in a 10% solution of neutral formalin. Subsequently, paraffin sections were prepared according to the generally accepted histotechnical method and stained with hematoxylin - eosin; according to Van Gieson and Mallory, an immunohistochemical study was used to study microenvironment factors. Results and Discussion. Immunohistochemical examination of the intestine was carried out in several stages, at the first stage we took intestinal tissue during the surgery to form the intestinal anastomosis, before the introduction of allogeneic cell transplants. Also carried out immunohistochemical studies of the emerging mucous membrane at various times after the formation of insolvency of intestinal anastomoses in both studied groups. When using a cell allograft, the mechanisms of repair of the intestinal mucosa include the appearance of cells that carry the expression of markers of mesenchymal and epithelial differentiation (Vim, CD34, CKW, Ecad), and are usually localized in the walls of blood vessels of granulation tissue, as well as in areas of the repairing epithelium. In addition, in the main group, only 7 days after the reaction to cytokeratins (CKW) and Ecad, areas of the repairing epithelium were detected, while the visualization of the epithelium in the control group did not begin even after 10 days. The analysis of immunophenotypes of repairing cells allows us to suggest the participation of mesenchymal-epithelial transformation mechanisms in this process. Conclusions.Thus, studies have shown that the use of cell transplantation of culture of allogeneic cells of the intestine leads to the reconstruction of the intestinal mucosa in a fairly short time. Conducted histological and immunohistochemical studies showed that in the vast majority of animals (77.2%) 10-14 days after the operation, an almost unchanged mucous membrane forms with the preservation of its elasticity.

scholarly journals MORPHOLOGY OF GLANDULAR AREA OF HARD PALATE MUCOSA IN INTACT ALBINO RATS

2020 ◽  
Vol 20 (2) ◽  
pp. 189-193
Author(s):  
Ye.S. Khilinich ◽  
V.Yu. Davydenko
Keyword(s):  
Salivary Glands ◽  
Mucous Membrane ◽  
Human Body ◽  
Hard Palate ◽  
Experimental Studies ◽  
Laboratory Animals ◽  
Albino Rats ◽  
Minor Salivary Glands ◽  
Palate Mucosa ◽  
The Impact

Some reports state that the morphology, histochemistry and innervation of mucous membrane in rats are quite similar to that in humans. Most experimental studies on the impact of certain factors on the human body involve laboratory animals, rats in particular. In our previous experimental studies we used rats to study the effect of acrylic monomer on salivary glands in order to further extrapolate data to the morphological features of minor salivary glands of rats and humans. This study was aimed at investigating morphology of glandular area of the hard palate mucosa of intact albino rats with subsequent extrapolation of the results obtained to human body. The experimental studies were conducted on adult Wistar rats aged 1 to 1.5 years. The light microscopy (slight magnification) of transverse sections of the hard palate mucosa samples of albino rats revealed the mucous membrane and well-developed submucous layer with numerous minor salivary glands within its structure. The findings confirm the similarity between the structure of minor salivary glands of rats and humans that supports the rational choice of experimental animals for subsequent extrapolation of the resulting data.

scholarly journals THE EVALUATION OF THE EFFECTIVENESS OF LOCAL APPLICATION OF OZONE THERAPY FOLLOWING CRYOTHERAPY OF LESIONS OF VERRUCOUS LEUKOPLAKIA OF THE ORAL MUCOUS MEMBRANE

2017 ◽  
Vol 22 (4) ◽  
pp. 184-187
Author(s):  
Svetlana I. Tokmakova ◽  
O. V Bondarenko ◽  
J. N Shilova
Keyword(s):  
Mucous Membrane ◽  
Postoperative Period ◽  
Local Treatment ◽  
Clinical Signs ◽  
Laboratory Animals ◽  
Control Group ◽  
Ozone Therapy ◽  
Mucosal Membrane ◽  
Traditional Scheme ◽  
Clinical Changes

There are presented results of the study morphological and clinical changes of the oral mucosal membrane. In the experiment on laboratory animals the use of ozone therapy following the destruction of the mucous membrane of the mouth was proved to appear earlier with the more complete epithelization of the defect, compared to the treatment implemented according to the traditional scheme. There was performed a clinical examination of 32 patients with verrucous leukoplakia of the oral mucosal membrane, the treatment of which was carried out according to the traditional scheme (cryotherapy and topical anti-inflammatory treatment - group comparison) and with the use of the ozonized solution of olive oil with a known peroxide during the postoperative period (control group). To assess the effectiveness of the proposed method of the management of the postoperative wound there was used a point scoring system of clinical signs at stages of the postoperative period. There was revealed a more earlier onset of the complete epithelization in cases of the application of the local ozone therapy in comparison with traditional local treatment.

scholarly journals USING MESENCHYMAL STEM CELLS FOR TREATING GINGIVAL RECESSION IN THE EXPERIMENT

2018 ◽  
Vol 25 (5) ◽  
pp. 83-92
Author(s):  
S. P. Rubnikovich ◽  
Yu. L. Denisova ◽  
V. A. Andreeva ◽  
G. Yu. Panasenkova ◽  
I. S. Khomich
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Experimental Studies ◽  
Gingival Recession ◽  
Laboratory Animals ◽  
Control Group ◽  
Periodontal Tissues ◽  
White Rats ◽  
Clinical Changes ◽  
Complete Regeneration

Aim. This research was conducted to study the type of clinical changes in the diseased periodontal tissues by means of using mesenchymal stem cells for treating gingival recession in the experiment.Materials and methods. Adipose tissue in a volume of 1-2 ml was sampled from one specimen in sterile conditions in order to obtain allogeneic mesenchymal stem cells before the beginning of the experimental studies. Random-bred female white rats (60 specimens) were used as a model in the experiment. The surgical intervention was performed in the area of incisors on the upper and lower jaws of rats. All animals were divided into 5 groups (10 rats in each group) depending on the planned method of the treatment. The control group, intact rats, consisted of 10 laboratory animals with a healthy gingiva. The creation of a model of the experimental gingival recession was carried out by the mechanical V-shaped excision of periodontal tissues.Results. The experimental studies showed the possibility of complete regeneration of the dentogingival papillae as well as periodontal tissues regeneration in the area of the experimental gingival recession during the first 28 days. Meanwhile, there is a complete absence of inflammatory signs in the 5th group (hyperemia, edema, gingival bleeding), along with their significant decrease in the 3rd and the 4th groups that confirms the pronounced therapeutic effect of mesenchymal stem cells and sterile bioplastic collagen material «Collost» (7% gel).The conclusion. While studying the type of clinical changes in diseased periodontal tissues using mesenchymal stem cells in the treatment of gingival recession, we established the positive dynamics in the experiment. The decrease in the intensity and prevalence of gingival inflammation starts from the 14th day. On the 28th day there was noted a complete absence of inflammatory signs in the animals after injecting the transplant consisting of the cell suspension on the sterile bioplastic material Collost (7% gel). Furthermore, the significant increase in the regeneration of the gingival margin was observed on the 24th day from the moment of creating the gingival recession model. The complete regeneration of the dentogingival papillae and the absence of the gingival recession were noted on the 38th day of general observation. 

scholarly journals Intestinal mucosal lymphocytes in neonatal sepsis

2019 ◽  
Vol 64 (4) ◽  
pp. 52-59
Author(s):  
Kh. S. Khaertynov ◽  
V. A. Anokhin ◽  
G. R. Burganova ◽  
G. O. Pevnev ◽  
M. O. Mavlikeev ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Large Intestine ◽  
Intestinal Mucosa ◽  
Neonatal Sepsis ◽  
Morphological Changes ◽  
Intestinal Bacteria ◽  
Main Group ◽  
Control Group ◽  
Small Intestinal Mucosa ◽  
Cd4 Lymphocytes

We studied the autopsy material obtained from 7 children who died in the neonatal period in order to evaluate the composition of lymphocytes of the intestinal mucosa against the background of morphological changes in the tissues of the gastrointestinal tract in newborns with sepsis. The main group consisted of 4 children with neonatal sepsis, the control group – of 3 newborns who died from other causes. The research material included the specimen of the small and large intestine.Results. Small intestine: it was found that there were less CD4 + lymphocytes in the small intestinal mucosa in the group of children who died from neonatal sepsis in 75% of cases than in the control group, but this difference was not statistically significant (p=0.1). There were no differences in the number of CD8 + and CD20 + cells in the studied groups. Large intestine: the number of CD4 + lymphocytes of the mucous membrane of the colon was greater in the main group of children than in the control group (p=0.03). An increase in the number of CD4 + cells was registered in 3 of 4 cases of neonatal sepsis. The number of CD8+ and CD20+ lymphocytes in the studied groups was the same (р>0.05).Conclusion. The increase in T-lymphocytes CD4+ in the mucous membrane of the large intestine is probably connected with the antigenic stimulation of opportunistic intestinal bacteria. We found no morphological signs of the suppression of the cells of adaptive immunity associated with the intestinal mucosa. 

scholarly journals Efficacy of application of a human placenta hydrolysate in prophylaxis of the intestinal anastomoses sutures insufficiency

2019 ◽  
Vol 86 (7) ◽  
pp. 9-12
Author(s):  
E. K. Aghayev ◽  
T. E. Mamedov ◽  
E. M. Gasimov ◽  
Z. E. Ismayilova
Keyword(s):  
Human Placenta ◽  
Standard Treatment ◽  
Intestinal Anastomosis ◽  
Main Group ◽  
Experimental Investigations ◽  
Control Group ◽  
Intestinal Anastomoses ◽  
Clinical Investigations ◽  
Intestinal Segments ◽  
Group A

Objective. Studying of impact of a human placenta hydrolysate on regeneration of intestinal anastomosis and prophylaxis of their sutures insufficiency. Materials and methods. Experimental investigations were conducted on two groups of rabbits. In every group a simulation model of an acute strangulation ileus was created. In a one day a relaparotomy, resection of necrotized intestinal segments with anastomosing in a “side-to-side” fashion were done in rabbits of both groups. After the operation the control group rabbits have obtained a standard treatment, while in the main group the rabbits together with a standard treatment have obtained a human placenta hydrolysate preparation «Laennec». On the days 3, 5, 7 and 15th the according intestinal segments were probed for morpho-histochemical investigations. Clinical investigations were conducted in 122 patients, consisting of resection of intestinal segments with formation of anastomoses. The control group consisted of 60 patients, and the main one – in 62. The data of the control group were studied retrospectively. In the main group the patients, together with a standard treatment, a human placenta hydrolysate in a form of preparation “Laennec” was applied for prophylaxis of insufficiency of the intestinal anastomoses sutures. Results. Basing on experimental results it may be stressed, that application of preparation «Laennec» strengthens regeneration and angiogenesis in zone of anastomosis, because in animals of the main group, comparing with a control one, high mitotic index, good angiogenesis and the motor-evacuation function integrity were noted. In clinical practice the anastomotic sutures insufficiency in the control group have occurred in 13.3% of observations, and in the main group – in 1.6% of observations. Conclusion. Application of the human placenta hydrolysate for prophylaxis of the sutures insufficiency in intestinal anastomoses is affordable due to successive clinical and experimental data obtained.

scholarly journals USE OF FATTY STROMAL CELLS IN THE COMPLEX TREATMENT OF TROPHIC ULCERS IN THE EXPERIMENT

2021 ◽  
pp. 14-22
Author(s):  
Volodymyr Lupaltsov ◽  
Andriy Yagniuk ◽  
Sergii Kitchenko ◽  
Irina Sorokina ◽  
Oksana Kaluzhina
Keyword(s):  
Adipose Tissue ◽  
Drug Therapy ◽  
Stromal Cells ◽  
Morphological Study ◽  
Venous Insufficiency ◽  
Autologous Transplantation ◽  
Experimental Studies ◽  
Control Group ◽  
Immunohistochemical Studies ◽  
Adipose Tissue Cells

Abstract. The aim of the study. The purpose of the research is to study the effectiveness of the treatment of trophic ulcers by autologous transplantation of stromal cells from adipose tissue in the experiment. Materials and methods of research. Experimental studies of the effectiveness of the treatment of trophic ulcers of venous etiology by autotransplantation of adipose tissue cells on 14 rabbits of the Chinchilla line, aged more than 4 months in both sexes, weighing 3 kilograms, are presented. After receiving the trophic ulcer, the rabbits were divided into 2 groups. A control group of 7 rabbits who used drug therapy and a core group of 7 rabbits who underwent autotransplantation of stromal cells from adipose tissue against the background of drug therapy. Both groups performed morphological and immunohistochemical studies. Results and conclusions. It is determined that the use of the method of autotransplantation of the stromo-vascular fraction of adipose tissue (SVF) in the treatment of trophic ulcer of venous etiology in the experiment in rabbits leads to acceleration of the processes in the healing of trophic ulcer, which is increased by the level of cytokyntinucleation, into more mature connective tissue. Keywords: mesenchymal stromal cells from adipose tissue, trophic ulcer, chronic venous insufficiency, experiment, morphological study, immunohistochemically research.

scholarly journals Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment

2021 ◽  
Vol 6 (5) ◽  
pp. 184-195
Author(s):  
D. V. Plotkin ◽  
T. I. Vinogradova ◽  
M. N. Reshetnikov ◽  
Yu. R. Zyuzya ◽  
S. V. Okovityi ◽  
...  
Keyword(s):  
Abdominal Cavity ◽  
Experimental Studies ◽  
Tuberculous Peritonitis ◽  
Lung Damage ◽  
Laboratory Animals ◽  
Control Group ◽  
Peritoneal Tuberculosis ◽  
Leading Role ◽  
Tnf Α

Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity. 

P2575Excess and deficiency of serotonin in embryogenesis affects the contractility of the right ventricle myocardium

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
F Mindubayeva ◽  
R Nedorezova ◽  
R Nigmatullina ◽  
M Akhmetova
Keyword(s):  
Right Ventricle ◽  
Myocardial Contractility ◽  
Experimental Studies ◽  
Control Group ◽  
Serotonin Metabolism ◽  
Force Of Contraction ◽  
Embryonic Period ◽  
Serotonin Deficiency ◽  
Series Of Experiments ◽  
The Right

Abstract Background Congenital heart disease (CHD) is one of the most common pathologies among congenital malformations in children. The incidence of CHD is currently more than 30% of all malformations. The most frequent and severe complication of CHD is pulmonary hypertension (PH). PH worsens the CHD prognosis, affects the survival rate of children, and increases the risk of disability and mortality. Experimental studies demonstrate a high correlation between the PH degree, concentrations of serotonin and its metabolites in the blood. The purpose of our study was to investigate the effect of excess and lack of serotonin in the embryonic period on the functioning of the heart in early postnatal ontogenesis. Methods Study was conducted according to “Principles of laboratory animal care”. The study was carried out on pregnant female Wistar rats and their offspring at the age of 14 days. Myocardial contractility in vitro was studied on myocardial strips of right ventricle. The amplitude-time characteristics of the contraction were calculated by the method of S. Laer 1998. The following series of experiments were conducted: 1. Pregnant females, starting from the 11th day of pregnancy for 10 days were injected intraperitoneally: group 1 - serotonin synthesis inhibitor (p-chlorophenylalanine) at a dose of 100 mg/kg; group 2 - selective serotonin reuptake inhibitor (fluoxetine) at a dose of 50 mg/kg; group 3 - (control group) - saline solution. Inotropic myocardial function was studied in the offsprings of each group at the age of 14 days. The reactions of the contraction of myocardial stripes of rat pups were evaluated in 2 batches: first on epinephrine and then on the calcium channel inhibitor (verapamil) at concentrations of 2.5 μM and in the reverse order. Results Our experiment demonstrates that the reaction of inotropic function of the right ventricle on epinephrine in 2 weeks of age rats is not affected by changes in serotonin metabolism in the embryonic period of development. Against the background of a pronounced positive effect from the use of epinephrine, verapamil was used to inhibit calcium channels. We observed a significant reduction in the force of contraction by 83.41±3.14% in the control group, by 71.42±2.95% in the group with an excess of serotonin and by 63.97±7.93% in group with serotonin deficiency. In the next series of experiments, verapamil was first applied, resulting the decrease of contraction strength by 78.58±1.71% in the control group, by 80.81±7.33% in the myocardium of rats with an excess of serotonin and by 67.70±4, 66% in the group with serotonin deficiency. Subsequent exposure of epinephrine led to an increase in myocardial contractility equally in all groups at 132–135%. Conclusion Violation of serotonin metabolism during embryogenesis affects the regulation of contractility (force of contraction) of the right ventricle. Acknowledgement/Funding This work was supported by the Ministry of Science and Education of Republic of Kazakhstan [grant number AP05136034]

IMPREGNATION OF ORAL MUCOSA OVER IMPACTED TEETH BY SUBPOPULATIONS OF MACROPHAGES M1 AND M2

2021 ◽  
Vol 74 (6) ◽  
pp. 1451-1456
Author(s):  
Pavlo I. Tkachenko ◽  
Maryna І. Dmytrenko ◽  
Mykola О. Cholovskyi ◽  
Lidiia D. Korovina ◽  
Tetiana V. Mamontova
Keyword(s):  
Diagnostic Criteria ◽  
Lamina Propria ◽  
Mucous Membrane ◽  
Oral Mucosa ◽  
Clinical Situation ◽  
Control Group ◽  
M2 Macrophages ◽  
Impacted Teeth ◽  
Cd 68 ◽  
Immunohistochemical Studies

The aim: Of the study is to research quantitative parameters of mucous membrane macrophages populations M1 (CD68+) and M2 (CD163+) over vestibularly and palatally impacted teeth. Materials and methods: A group of 21 people aged from 10 to 16 years was formed to conduct the research. Clinical situation according to diagnostic criteria was identical in all the patients. The group was divided into two groups - control and experimental, which in their turn were fragmented into two subgroups. Immunohistochemical studies of mucosal biopsies were performed in accordance with the recommendations for selection. Results: Study of ratio of CD68+/CD163+ cells revealed imbalance in individuals with vestibularly impacted teeth due to higher infiltration density of CD163+ (p<0,05), compared to CD68+ of control group. In individuals with palatally impacted teeth, ratio of CD68+/CD163+ increased 3,6 times, as well as compared with control group, but due increased infiltration density of CD68+. Conclusions: In the epithelium of oral mucosa located over impacted teeth, both on vestibular and palatal surface, number of CD 68+ and CD163+ cells had no significant differences compared to control group. In biopsies of the lamina propria of mucosa over vestibularly impacted teeth, the ratio M1/M2=0,91±0,11 (p<0,05) decreases, with predominance of macrophages CD163+ subpopulation activity, and over palatally impacted teeth balance of M1/ M2 macrophages elevated (M1/M2= 2,10 ± 0,32, p<0,05), due to increased infiltration density of CD68+.

scholarly journals Troponins in Experimental Studies

2002 ◽  
Vol 45 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Michaela Adamcová ◽  
Vladimír Geršl ◽  
Jarmila Macháčková ◽  
Radomír Hrdina ◽  
Ivona Klimtová ◽  
...  
Keyword(s):  
Cardiac Troponin ◽  
Diagnostic Performance ◽  
Troponin I ◽  
Troponin T ◽  
Experimental Studies ◽  
Repeated Administration ◽  
Treated Group ◽  
Laboratory Animals ◽  
Coefficient Of Determination ◽  
Control Group

The aim of our study was to compare the diagnostic performance of cardiac troponin T (cTnT) and cardiac troponin I (cTnI) in three groups of rabbits: 1) control (saline 1 ml/kg i.v.); 2) daunorubicin (3 mg/kg i.v.); 3) daunorubicin (3 mg/kg i.v.) + dexrazoxane (60 mg/kg i.p.). The drugs were given once a week, 10 administrations. The concentration of cTnT was measured using Elecsys Troponin T STAT Immunoassay (Roche). The concentration of cTnI was measured using AxSYM Troponin I (Abbott). The linear regression model was applied to see if there is a dependence between cTnT and cTnI. The coefficient of determination (R2 = 0.79) was acceptable only in the control group. In the remaining cases (i.e. in the daunorubicin group and in the daunorubicin + dexrazoxane treated group) R2 was too small (0.53, and 0.06). We may conclude that in rabbits after repeated administration of cardiotoxic or cardioprotective drugs meaningful dependence between cTnT and cTnI was not found. The choice of the most suitable cardiomarker in laboratory animals deserves further studies.

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