Germinación de semillas de quina, Cinchona pubescens Vahl. con ácido giberélico, nitrato de postasio y agua de coco

2016 ◽  
Vol 4 (1) ◽  
pp. 13
Author(s):  
Joseph Campos Ruiz

Cinchona pubscens Vahl. es una especie forestal de gran valor medicinal, comercial y ecológica que se encuentra distribuida desde Costa Rica hasta el Perú y que, por la alteración de su hábitat y la difícil germinación de sus semillas en campo, se encuentra en peligro de extinción. En este trabajo se evaluó el efecto del ácido giberélico, nitrato de potasio y agua de coco en la germinación de sus semillas en material colectado en las montañas del distrito de Colasay (Jaén, Perú). El nitrato de potasio actúo como un promotor en la aceleración de la germinación de las semillas. La concentración 1000 ppm brindó el más alto porcentaje de germinación, energía germinativa y valor de germinación; en cambio, el tratamiento con AG3 a las concentraciones de 1000, 2000 y 3000 ppm inhibió la germinación de estas semillas; y no se justifica una alta inversión en la adquisición del ácido giberélico, cuando se puede implementar un sistema fácil y económico, como es el tratamiento con KNO3

2021 ◽  
Author(s):  
David Clements

Abstract C. pubescens is a source of the drug quinine, used to control the effects of malaria, and Cinchona bark was being used in Europe as a treatment for malaria as early as the 1650s, before the plant was authenticated botanically (Raintree, 2003). The Jesuits had introduced it from the New World, hence the name Jesuit's bark or powder (Prendergast and Dolley, 2001). The bark was collected in its native Andean range and probably comprised a mixture of species including C. pubescens. The native range of C. pubescens extends from Andean South America in Bolivia north to Costa Rica. It was widely distributed throughout the tropics as a source of quinine, particularly by the British during the latter half of the 1800s (Moureau, 1945) and is probably still held in many tropical botanical gardens worldwide for its medicinal interest.


Author(s):  
O. E. Bradfute

Maize rayado fino virus (MRFV) causes a severe disease of corn (Zea mays) in many locations throughout the neotropics and as far north as southern U.S. MRFV particles detected by direct electron microscopy of negatively stained sap from infected leaves are not necessarily distinguishable from many other small isometric viruses infecting plants (Fig. 1).Immunosorbent trapping of virus particles on antibody-coated grids and the antibody coating or decoration of trapped virus particles, was used to confirm the identification of MRFV. Antiserum to MRFV was supplied by R. Gamez (Centro de Investigacion en Biologia Celular y Molecular, Universidad de Costa Rica, Ciudad Universitaria, Costa Rica).Virus particles, appearing as a continuous lawn, were trapped on grids coated with MRFV antiserum (Fig. 2-4). In contrast, virus particles were infrequently found on grids not exposed to antiserum or grids coated with normal rabbit serum (similar to Fig. 1). In Fig. 3, the appearance of the virus particles (isometric morphology, 30 nm diameter, stain penetration of some particles, and morphological subunits in other particles) is characteristic of negatively stained MRFV particles. Decoration or coating of these particles with MRFV antiserum confirms their identification as MRFV (Fig. 4).


2001 ◽  
Vol 60 (2) ◽  
pp. 89-98 ◽  
Author(s):  
Alain Clémence ◽  
Thierry Devos ◽  
Willem Doise

Social representations of human rights violations were investigated in a questionnaire study conducted in five countries (Costa Rica, France, Italy, Romania, and Switzerland) (N = 1239 young people). We were able to show that respondents organize their understanding of human rights violations in similar ways across nations. At the same time, systematic variations characterized opinions about human rights violations, and the structure of these variations was similar across national contexts. Differences in definitions of human rights violations were identified by a cluster analysis. A broader definition was related to critical attitudes toward governmental and institutional abuses of power, whereas a more restricted definition was rooted in a fatalistic conception of social reality, approval of social regulations, and greater tolerance for institutional infringements of privacy. An atypical definition was anchored either in a strong rejection of social regulations or in a strong condemnation of immoral individual actions linked with a high tolerance for governmental interference. These findings support the idea that contrasting definitions of human rights coexist and that these definitions are underpinned by a set of beliefs regarding the relationships between individuals and institutions.


2010 ◽  
Vol 30 (S 01) ◽  
pp. S28-S31 ◽  
Author(s):  
J. Arroyo ◽  
L. Salazar-Sánchez ◽  
G. Jiménez-Cruz ◽  
P. Chaverri ◽  
E. Arrieta-Bolaños ◽  
...  

SummaryHaemophilia is the most frequent hereditary haemorrhagic illness and it is due to the deficiency of coagulation factors VIII (haemophilia A, HA) or IX (haemophilia B, HB).The prevalence of this disease varies according to the country, those having better survival rates having also higher prevalences. Specifically in Costa Rica, there are around 130 HA and 30 HB families. This study reports the prevalence and a spatial distribution analysis of both types of the disease in this country. The prevalence of haemophilia in this country is 7 cases per 100 000 men, for HA it is 6 cases per 100 000 and for HB it is 1 case per 100 000 male inhabitants. The prevalence of this disease is low when compared with other populations. This low prevalence could be due to the many patients that have died because of infection with human immunodeficiency virus during the 1980s. The prevalence of haemophilia in Costa Rica is almost one half of that present in developed countries. Nevertheless, the ratio between HA and HB follows world tendency: 5 : 1. In this study, nationwide geographical distribution maps were drawn in order to visualize the origin of severe cases and how this influences the pattern of distribution for both types of haemophilia. By means of these maps, it was possible to state that there is no association between the sites of maximum prevalence of mutated alleles and ethnicity. With this study, haemophilia prevalence distribution maps can be used to improve efforts for the establishment of hemophilia clinics or specialized health centers in those areas which hold the highest prevalences in this country. Also, this knowledge can be applied to improve treatment skills and offer the possibility of developing focused genetic counseling for these populations.


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