scholarly journals AN INVESTIGATION TOWARDS DEVELOPING A MOLECULAR APPROACH TO IMPROVE THE EFFICIENCY OF COCONUT BREEDING BY RAPD-MARKER ASSISTED SELECTION

CORD ◽  
1999 ◽  
Vol 15 (02) ◽  
pp. 34
Author(s):  
J.M.D.T. Everard
Keyword(s):  
Molecular Markers ◽  
Sri Lanka ◽  
Marker Assisted Selection ◽  
Rapd Marker ◽  
Molecular Approach ◽  
Economic Traits ◽  
Random Primers ◽  
Rapd Pcr ◽  
Pcr Products ◽  
Hybrid Seeds

Tall, dwarf green and San Ramon are three phenotypically distinct forms of coconut, currently used for production of hybrid seeds in Sri Lanka. Development of molecular markers for further improvement of important economic traits of these hybrids was thought important due to various constraints faced by conventional breeders. Sixty random primers (OPERON) were used for generation of RAPD profiles using DNA from 3 individuals of each form. All the sixty primers except one (OPC3) yielded RAPD-PCR products with coconut DNA. They generated a total of 326 consistently amplified bands of which 54 distinguished tall, dwarf green and San Ramon. Among RAPDs detected 16 and 8 were specific to tall and dwarf respectively, 18 distinguished dwarf and San Ramon from tall, 9 distinguished tall and San Ramon from dwarf and 3 distinguished tall and dwarf green from San Ramon. It is expected to study the segregation of these markers in F2 populations derived from tall x dwarf green and tall x San Ramon F1 palms.

scholarly journals A Comparative Study of 5S rDNA Non-Transcribed Spacers in Elaeagnaceae Species

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 4
Author(s):  
Oleg S. Alexandrov ◽  
Olga V. Razumova ◽  
Gennady I. Karlov
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Markers ◽  
Dna Markers ◽  
5S Rdna ◽  
Chain Reaction ◽  
Closely Related Species ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Species Specific ◽  
Shepherdia Canadensis

5S rDNA is organized as a cluster of tandemly repeated monomers that consist of the conservative 120 bp coding part and non-transcribed spacers (NTSs) with different lengths and sequences among different species. The polymorphism in the 5S rDNA NTSs of closely related species is interesting for phylogenetic and evolutional investigations, as well as for the development of molecular markers. In this study, the 5S rDNA NTSs were amplified with universal 5S1/5S2 primers in some species of the Elaeagnaceae Adans. family. The polymerase chain reaction (PCR) products of five Elaeagnus species had similar lengths near 310 bp and were different from Shepherdia canadensis (L.) Nutt. and Sh. argentea (Pusch.) Nutt. samples (260 bp and 215 bp, respectively). The PCR products were cloned and sequenced. An analysis of the sequences revealed that intraspecific levels of NTS identity are high (approximately 95–96%) and similar in the Elaeagnus L. species. In Sh. argentea, this level was slightly lower due to the differences in the poly-T region. Moreover, the intergeneric and intervarietal NTS identity levels were studied and compared. Significant differences between species (except E. multiflora Thunb. and E. umbellata Thunb.) and genera were found. Herein, a range of the NTS features is discussed. This study is another step in the investigation of the molecular evolution of Elaeagnaceae and may be useful for the development of species-specific DNA markers in this family.

scholarly journals Analysis of Genetic Diversity of Indonesia Rodent Tuber (Typhonium flagelliforme Lodd.) Cultivars Based on RAPD Marker

2015 ◽  
Vol 2 (1) ◽  
pp. 139
Author(s):  
Danny Laurent ◽  
Nesti F. Sianipar ◽  
Chelen _ ◽  
Listiarini _ ◽  
Ariandana Wantho
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Medicinal Plant ◽  
Genetic Distance ◽  
Genetic Information ◽  
Rapd Marker ◽  
Anti Cancer ◽  
Cancer Agent

<p>Rodent tuber (Typhonium flagelliforme Lodd.) is a plant from Araceae family. The plant has high medical potential as anti-cancer agent. The information regarding Indonesian rodent tuber’s genetic diversity is not available yet. Genetic information is very important for the development of rodent tuber as medicinal plant. In this research, genetic diversity and genetic distance of three Indonesian rodent tuber’s cultivars, from Bogor, Pekalongan, and Medan, were analyzed by using RAPD molecular markers. The data obtained was analyzed by NTsys software. Out of 16 primers used in the study, the 12 primers were found to be polymorphic. There were 83 bands of DNA obtained and 31 of them were polymorphic. Dendogram analysis of the three rodent tuber cultivars showed that these cultivars were clustered into two clusters. The first cluster consists of rodent tuber Bogor and Medan. The second cluster consists of rodent tuber Pekalongan. The coefficient of similarity ranged from 0.81 to 0.87. The highest coefficient of similarity was 0.87, which was detected between rodent tuber Pekalongan and Medan. The lowest coefficient of similarity was 0.81, which was detected between rodent tuber Bogor and Pekalongan. Among these three cultivars of rodent tuber, cultivar Bogor was exclusively different.</p><p><br /><strong>Keywords</strong>: Indonesia-rodent tuber, genetic diversity, RAPD-marker</p>

scholarly journals Validation of molecular markers for the analysis of genetic diversity of amylase content and gel consistency among representative rice varieties in Sri Lanka

2015 ◽  
Vol 26 (2) ◽  
pp. 317 ◽  
Author(s):  
H.K.D.H. Fernando ◽  
T.J.C. Kajenthini ◽  
S.P. Rebeira ◽  
T.C. Bamunuarachchige ◽  
H.A.M. Wickramasinghe
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Sri Lanka ◽  
Rice Varieties ◽  
Gel Consistency

scholarly journals RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis

2012 ◽  
Vol 39 (No. 1) ◽  
pp. 33-37 ◽  
Author(s):  
Y. Ii ◽  
A. Uragami ◽  
Y. Uno ◽  
M. Kanechi ◽  
N. Inagaki
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Genomic Dna ◽  
Asparagus Officinalis ◽  
Breeding Line ◽  
Male And Female ◽  
Future Studies ◽  
Random Primers

Asparagus (Asparagus officinalis L.) plants are dioecious. All-male cultivars are desired because of their higher yields. To increase the proportion of male individuals planted in the field and expedite the breeding of all-male cultivars in asparagus, development of generally applicable molecular markers to distinguish male and female individuals is required. Bulked genomic DNA samples from ten male (XY) and ten female (XX) plants was screened with 10-bp random primers. Of the 188 primers tested, the primer T35R54 produced a 1600-bp fragment observed only in male individuals. The specificity of this T35R54-1600 marker was verified using DNA from one supermale (YY) and one female (XX) breeding line and their four F<sub>1</sub> progenies (XY). The T35R54-1600 marker fragment was observed in both supermale and all-male lines. The sequence of the T35R54 primer (5'-TTCACGGTGG-3') was absent among the sequences of primers or amplified fragments from previous studies. Therefore, this marker could be useful as a sex-related marker in future studies to increase the reliability of sex determination in asparagus.

scholarly journals Marker Assisted Selection (MAS) for chickpea Fusarium oxysporum wilt resistant genotypes using PCR based molecular markers

2014 ◽  
Vol 41 (10) ◽  
pp. 6755-6762 ◽  
Author(s):  
Zakia Ahmad ◽  
Abdul Samad Mumtaz ◽  
Abdul Ghafoor ◽  
Amjad Ali ◽  
Mohammad Nisar
Keyword(s):  
Molecular Markers ◽  
Fusarium Oxysporum ◽  
Marker Assisted Selection ◽  
Resistant Genotypes

Molecular characterization and SNP identification in HSPB1 gene in Murrah buffalo

2015 ◽  
Author(s):  
Ashwani Arya ◽  
Archana Verma ◽  
I. D. Gupta ◽  
Shahid A. Shergojry ◽  
Ankit Magotra ◽  
...  
Keyword(s):  
Heat Shock ◽  
Marker Assisted Selection ◽  
Bos Taurus ◽  
Nucleotide Position ◽  
Coding Region ◽  
Murrah Buffalo ◽  
Future Improvement ◽  
Pcr Products ◽  
Exon 1 ◽  
Heat Shock Protein Genes

Present study was conducted on 100 lactating Murrah buffaloes maintained at LRC, NDRI Karnal (Haryana) to characterize and to identify genetic polymorphisms in HSPB1 gene. Two sets of primers specific to coding sequence of HSPB1 gene were designed using Primer3 software and PCR products of 631 bp and 670 bp were obtained. Amplicons were custom sequenced and subjected to ClustalW analysis which revealed 8 nucleotide changes, 7 in non coding region and one in coding region in Murrah buffalo sequence as compared to Bos taurus. Only one SNP at nucleotide position G225A in exon 1 of HSPB1 gene was observed in Murrah buffalo, which resulted in two genotypes GG and AG with respective frequency of 0.84 and 0.16 indicating the existence of variability in the sampled population. The study has opened the possibility of identifying and using genetic variations in major heat shock protein genes, for the future improvement of livestock through marker assisted selection.

Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat

1994 ◽  
Vol 87 (7) ◽  
pp. 789-794 ◽  
Author(s):  
L. E. Talbert ◽  
N. K. Blake ◽  
P. W. Chee ◽  
T. K. Blake ◽  
G. M. Magyar
Keyword(s):  
Molecular Markers ◽  
Sequence Tagged Site ◽  
Pcr Products

DEVELOPING MOLECULAR MARKERS FOR MARKER ASSISTED SELECTION OF FIRE BLIGHT RESISTANT APPLE SEEDLINGS

2007 ◽  
pp. 117-122 ◽  
Author(s):  
A. Peil ◽  
M.-V. Hanke ◽  
H. Flachowsky ◽  
K. Richter ◽  
T. Garcia ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Marker Assisted Selection ◽  
Fire Blight ◽  
Selection Of
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