Abstract Generally, investigations on nanomedicine involve conventional
imaging techniques for obtaining static images on nanoparticle
internalization at a single time point where various phases can be
overlooked. In contrast, 3D live-cell imaging can be used for obtaining
cellular retention of drugs at various phases, and cells can be followed
for days. This article demonstrates the application of time-lapse
microscopy in the investigation of Poly-L-lysine coated ZnO nanoparticle
dynamics. In this work, a laser scanning confocal microscope has been
employed to quantify the dynamics of internalization particles and
reactive oxygen species generation (ROS) using volumetric imaging.
Firstly, we show that simultaneous spatial mapping of nanoparticle
uptake in MCF-7 cells and ROS in a single cell can be used to identify
the interdependence between the accumulation of particles and ROS
generation. Secondly, monitoring of ROS formation and cytotoxicity using
the same imaging platform offers an advantage over monitoring these
parameters using various instruments. Finally, the ability of the
fluorescent particles in inducing a significant reduction in cell
viability suggests its potential to be used as a therapeutic agent. The
proposed framework opens up a new avenue of research for investigating
mechanistic aspects of ZnO particle adsorption in vitro through long
term imaging. Keywords: Fluorescent ZnO particle, Time-lapse microscopy,
3D Live-cell imaging, laser scanning confocal microscope, Reactive
oxygen species
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
