scholarly journals An Ex vivo Culture System to Study Thyroid Development

10.3791/51641 ◽  
2014 ◽  
Author(s):  
Anne-Sophie Delmarcelle ◽  
Mylah Villacorte ◽  
Anne-Christine Hick ◽  
Christophe E. Pierreux
Keyword(s):  
Culture System ◽  
Ex Vivo ◽  
Thyroid Development

Establishment of a Novel Intervertebral Disc/Endplate Culture Model: Analysis of an Ex Vivo In Vitro Whole-Organ Rabbit Culture System

Spine ◽  
2006 ◽  
Vol 31 (25) ◽  
pp. 2918-2925 ◽  
Author(s):  
Daniel Haschtmann ◽  
Jivko V. Stoyanov ◽  
Ladina Ettinger ◽  
Lutz -P. Nolte ◽  
Stephen J. Ferguson
Keyword(s):  
Intervertebral Disc ◽  
Culture System ◽  
Ex Vivo ◽  
Model Analysis ◽  
Culture Model

DESIGN OF AN EX-VIVO VESSEL CULTURE SYSTEM FOR THE STUDY OF EARLY ARTERIALIZATION PHENOMENA IN HUMAN SAPHENOUS BYPASS GRAFTS

2012 ◽  
Vol 45 ◽  
pp. S151
Author(s):  
Marco Piola ◽  
Monica Soncini ◽  
Francesca Prandi ◽  
Maurizio Pesce ◽  
Gianfranco Beniamino Fiore
Keyword(s):  
Culture System ◽  
Ex Vivo ◽  
Bypass Grafts

Establishing an ex vivo culture system for normal pancreatic tissue

Pancreatology ◽  
2014 ◽  
Vol 14 (3) ◽  
pp. S120-S121
Author(s):  
Carlos Fernández Moro ◽  
Sougat Misra ◽  
Soledad Pouso ◽  
Marita Wallenberg ◽  
Rainer Heuchel ◽  
...  
Keyword(s):  
Culture System ◽  
Ex Vivo ◽  
Pancreatic Tissue ◽  
Normal Pancreatic Tissue ◽  
Ex Vivo Culture

Bench-Top Validation Tests for Tissue-Engineered Arteries

2000 ◽  
Author(s):  
Shawn Chin Quee ◽  
Hai-Chao Han ◽  
David N. Ku
Keyword(s):  
Organ Culture ◽  
Culture System ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Living System ◽  
Organ Culture System ◽  
Flow Pressure ◽  
Validation Tests

Abstract Standard tests are needed for evaluating and comparing the mechanical and biological functions of tissue engineered arteries and other vascular grafts. We propose an ex vivo organ culture system as a living system for testing tissue-engineered vascular grafts. This bench-top organ culture system mimics the physiological environment of arteries including the flow, pressure, and the axial stretch. Arterial mechanical properties and physiologic functions including compliance, burst pressure, and contractile functions can be assessed before an expensive long-term animal test is initiated. Test results of natural arteries indicate that organ culture is a valid model for comprehensive evaluation of tissue-engineered vascular grafts.

Axial Stretch Increases Cell Proliferation in Arteries in Organ Culture

2000 ◽  
Author(s):  
Hai-Chao Han ◽  
Raymond P. Vito ◽  
Kristin Michael ◽  
David N. Ku
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Organ Culture ◽  
Vascular Function ◽  
Culture System ◽  
Carotid Arteries ◽  
Ex Vivo ◽  
Axial Stretch ◽  
Organ Culture System ◽  
Physiological Flow

Abstract To study the effect of axial stretch on vascular function and wall remodeling, porcine carotid arteries were cultured under conditions of physiological flow and elevated axial stretch in an ex vivo organ culture system. Smooth muscle cell proliferation was measured by bromodeoxyuridine index. Results showed that cell proliferation was significantly increased in the highly stretched arteries when compared to the normally stretched arteries. This may indicate the feasibility of stimulating new arterial growth by stretching natural arteries.

scholarly journals Evaluation of bone formation on orthopedic implant surfaces using an ex-vivo bone bioreactor system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rupak Dua ◽  
Hugh Jones ◽  
Philip C. Noble
Keyword(s):  
Culture System ◽  
Ex Vivo ◽  
Calcium Phosphates ◽  
Wire Mesh ◽  
Bioreactor Culture ◽  
Static Culture ◽  
Group 4 ◽  
The Matrix ◽  
Bioreactor System ◽  
Fiber Mesh

AbstractRecent advances in materials and manufacturing processes have allowed the fabrication of intricate implant surfaces to facilitate bony attachment. However, refinement and evaluation of these new design strategies are hindered by the cost and complications of animal studies, particularly during early iterations in the development process. To address this problem, we have previously constructed and validated an ex-vivo bone bioreactor culture system that can maintain the viability of bone samples for an extended period ex-vivo. In this study, we investigated the mineralization of a titanium wire mesh scaffold under both static and dynamic culturing using our ex vivo bioreactor system. Thirty-six cancellous bone cores were harvested from bovine metatarsals at the time of slaughter and divided into five groups under the following conditions: Group 1) Isolated bone cores placed in static culture, Group 2) Unloaded bone cores placed in static culture in contact with a fiber-mesh metallic scaffold, Group 3) Bone cores placed in contact with a fiber-mesh metallic scaffold under the constant pressure of 150 kPa, Group 4) Bone core placed in contact with a fiber-mesh metallic scaffold and exposed to cyclic loading with continuous perfusion flow of media within the ex-vivo culture system and Group 5) Bone core evaluated on Day 0 to serve as a positive control for comparison with all other groups at weeks 4 and 7. Bone samples within Groups 1–4 were incubated for 4 and 7 weeks and then evaluated using histological examination (H&E) and the Live-Dead assay (Life Technologies). Matrix deposits on the metallic scaffolds were examined with scanning electron microscopy (SEM), while the chemical composition of the matrix was measured using energy-dispersive x-ray spectroscopy (EDX). We found that the viability of bone cores was maintained after seven weeks of loading in our ex vivo system. In addition, SEM images revealed crystallite-like structures on the dynamically loaded metal coupons (Group 4), corresponding to the initial stages of mineralization. EDX results further confirmed the presence of carbon at the interface and calcium phosphates in the matrix. We conclude that a bone bioreactor can be used as an alternate tool for in-vivo bone ingrowth studies of new implant surfaces or coatings.

Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1623-1636 ◽  
Author(s):  
Chu-Chih Shih ◽  
Mickey C.-T. Hu ◽  
Jun Hu ◽  
Jeffrey Medeiros ◽  
Stephen J. Forman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Ex Vivo ◽  
Human Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Culture System

Abstract We have developed a stromal-based in vitro culture system that facilitates ex vivo expansion of transplantable CD34+thy-1+ cells using long-term hematopoietic reconstitution in severe combined immunodeficient-human (SCID-hu) mice as an in vivo assay for transplantable human hematopoietic stem cells (HSCs). The addition of leukemia inhibitory factor (LIF) to purified CD34+ thy-1+ cells on AC6.21 stroma, a murine bone marrow–derived stromal cell line, caused expansion of cells with CD34+ thy-1+ phenotype. Addition of other cytokines, including interleukin-3 (IL-3), IL-6, granulocyte-macrophage colony-stimulating factor, and stem cell factor, to LIF in the cultures caused a 150-fold expansion of cells retaining the CD34+ thy-1+ phenotype. The ex vivo–expanded CD34+ thy-1+ cells gave rise to multilineage differentiation, including myeloid, T, and B cells, when transplanted into SCID-hu mice. Both murine LIF (cannot bind to human LIF receptor) and human LIF caused expansion of human CD34+ thy-1+ cells in vitro, suggesting action through the murine stroma. Furthermore, another human HSC candidate, CD34+ CD38− cells, shows a similar pattern of proliferative response. This suggests thatex vivo expansion of transplantable human stem cells under this in vitro culture system is a general phenomenon and not just specific for CD34+ thy-1+ cells.

scholarly journals Ex vivo organotypic culture system of precision-cut slices of human pancreatic ductal adenocarcinoma

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sougat Misra ◽  
Carlos F. Moro ◽  
Marco Del Chiaro ◽  
Soledad Pouso ◽  
Anna Sebestyén ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Culture System ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Ductal Adenocarcinoma
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