scholarly journals Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

10.3791/57673 ◽  
2018 ◽  
Author(s):  
Hyunryul Ryu ◽  
Kyungyong Choi ◽  
Yanyan Qu ◽  
Taehong Kwon ◽  
Janet S. Lee ◽  
...  
Keyword(s):  
Closed Loop ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Airway Secretion

scholarly journals Patient-Derived Airway Secretion Dissociation Technique To Isolate and Concentrate Immune Cells Using Closed-Loop Inertial Microfluidics

2017 ◽  
Vol 89 (10) ◽  
pp. 5549-5556 ◽  
Author(s):  
Hyunryul Ryu ◽  
Kyungyong Choi ◽  
Yanyan Qu ◽  
Taehong Kwon ◽  
Janet S. Lee ◽  
...  
Keyword(s):  
Immune Cells ◽  
Closed Loop ◽  
Inertial Microfluidics ◽  
Airway Secretion

scholarly journals Microfluidic label-free bioprocessing of human reticulocytes from erythroid culture

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3445-3460
Author(s):  
Kerwin Kwek Zeming ◽  
Yuko Sato ◽  
Lu Yin ◽  
Nai-Jia Huang ◽  
Lan Hiong Wong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Closed Loop ◽  
Lateral Displacement ◽  
Label Free ◽  
Hematopoietic Stem ◽  
Deterministic Lateral Displacement ◽  
Dean Flow ◽  
Flow Fractionation ◽  
Human Rbcs

Developments in Dean flow fractionation (DFF) and deterministic lateral displacement (DLD) for label-free purification of cultured RBCs from human hematopoietic stem cells. An advancement in sorting and closed-loop manufacturing of viable human RBCs.

High-throughput rare cell separation from blood samples using steric hindrance and inertial microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2525-2538 ◽  
Author(s):  
Shaofei Shen ◽  
Chao Ma ◽  
Lei Zhao ◽  
Yaolei Wang ◽  
Jian-Chun Wang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Steric Hindrance ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Blood Samples ◽  
Rare Cells

We present a multistage microfluidic device for continuous label-free separation of rare cells using a combination of inertial microfluidics and steric hindrance.

scholarly journals Inertial Microfluidics Enabling Clinical Research

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 257
Author(s):  
Srivathsan Kalyan ◽  
Corinna Torabi ◽  
Harrison Khoo ◽  
Hyun Woo Sung ◽  
Sung-Eun Choi ◽  
...  
Keyword(s):  
Microfluidic Channel ◽  
Relevant Information ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Inertial Focusing ◽  
Viscous Forces ◽  
Solution Exchange ◽  
On Chip ◽  
Improving Accuracy ◽  
Hybrid Devices

Fast and accurate interrogation of complex samples containing diseased cells or pathogens is important to make informed decisions on clinical and public health issues. Inertial microfluidics has been increasingly employed for such investigations to isolate target bioparticles from liquid samples with size and/or deformability-based manipulation. This phenomenon is especially useful for the clinic, owing to its rapid, label-free nature of target enrichment that enables further downstream assays. Inertial microfluidics leverages the principle of inertial focusing, which relies on the balance of inertial and viscous forces on particles to align them into size-dependent laminar streamlines. Several distinct microfluidic channel geometries (e.g., straight, curved, spiral, contraction-expansion array) have been optimized to achieve inertial focusing for a variety of purposes, including particle purification and enrichment, solution exchange, and particle alignment for on-chip assays. In this review, we will discuss how inertial microfluidics technology has contributed to improving accuracy of various assays to provide clinically relevant information. This comprehensive review expands upon studies examining both endogenous and exogenous targets from real-world samples, highlights notable hybrid devices with dual functions, and comments on the evolving outlook of the field.

Label-Free Cancer Cell Separation from Human Whole Blood Using Inertial Microfluidics at Low Shear Stress

2013 ◽  
Vol 85 (13) ◽  
pp. 6213-6218 ◽  
Author(s):  
Myung Gwon Lee ◽  
Joong Ho Shin ◽  
Chae Yun Bae ◽  
Sungyoung Choi ◽  
Je-Kyun Park
Keyword(s):  
Shear Stress ◽  
Cancer Cell ◽  
Whole Blood ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Human Whole Blood ◽  
Free Cancer Cell ◽  
Low Shear Stress ◽  
Low Shear

scholarly journals Label-Free Enrichment of Adrenal Cortical Progenitor Cells Using Inertial Microfluidics

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46550 ◽  
Author(s):  
Soojung Claire Hur ◽  
Tatiana Z. Brinckerhoff ◽  
Christopher M. Walthers ◽  
James C. Y. Dunn ◽  
Dino Di Carlo
Keyword(s):  
Progenitor Cells ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Free Enrichment ◽  
Cortical Progenitor

scholarly journals High resolution and high throughput bacteria separation from blood using elasto-inertial microfluidics

2020 ◽  
Author(s):  
Sharath Narayana Iyengar ◽  
Tharagan Kumar ◽  
Gustaf Mårtensson ◽  
Aman Russom
Keyword(s):  
Sample Preparation ◽  
Blood Sample ◽  
High Throughput ◽  
Blood Cells ◽  
Separation Efficiency ◽  
Unmet Need ◽  
Outer Wall ◽  
Separation Performance ◽  
Label Free ◽  
Inertial Microfluidics

AbstractImproved sample preparation has the potential to address a huge unmet need for fast turnaround sepsis tests that enable early administration of appropriate antimicrobial therapy. In recent years, inertial and elasto-inertial microfluidics-based sample preparation has gained substantial interest for bioparticle separation applications. However, for applications in blood stream infections the throughput and bacteria separation efficiency has thus far been limited. In this work, for the first time we report elasto-inertial microfluidics-based bacteria isolation from blood at throughputs and efficiencies unparalleled with current microfluidics-based state of the art. In the method, bacteria-spiked blood sample is prepositioned close to the outer wall of a spiral microchannel using a viscoelastic sheath buffer. The blood cells will remain fully focused throughout the length of the channel while bacteria migrate to the inner wall for effective separation. Initially, particles of different sizes were used to investigate particle focusing and the separation performance of the spiral device. A separation efficiency of 96% for the 1 µm particles was achieved, while 100% of 3 µm particles were recovered at the desired outlet at a high throughput of 1 mL/min. Following, processing blood samples revealed a minimum of 1:2 dilution was necessary to keep the blood cells fully focus at the outer wall. In experiments involving bacteria spiked in diluted blood, viable E.coli were continuously separated at a total flow rate of 1 mL/min, with an efficiency between 82 to 90% depending on the blood dilution. Using a single spiral, it takes 40 minutes to process 1 mL of blood at a separation efficiency of 82% and 3 hours at 90% efficiency. To the best of our knowledge, this is the highest blood sample throughput per single microfluidic chip reported for the corresponding separation efficiency. As such, the label-free, passive and high throughput bacteria isolation method has a great potential for speeding up downstream phenotypic and molecular analysis of bacteria.

scholarly journals Label‐Free Isolation and Single Cell Biophysical Phenotyping Analysis of Primary Cardiomyocytes Using Inertial Microfluidics

Small ◽  
2020 ◽  
pp. 2006176
Author(s):  
Hossein Tavassoli ◽  
Prunella Rorimpandey ◽  
Young Chan Kang ◽  
Michael Carnell ◽  
Chris Brownlee ◽  
...  
Keyword(s):  
Single Cell ◽  
Label Free ◽  
Inertial Microfluidics

scholarly journals Heart on A Chip: Label‐Free Isolation and Single Cell Biophysical Phenotyping Analysis of Primary Cardiomyocytes Using Inertial Microfluidics (Small 8/2021)

Small ◽  
2021 ◽  
Vol 17 (8) ◽  
pp. 2170034
Author(s):  
Hossein Tavassoli ◽  
Prunella Rorimpandey ◽  
Young Chan Kang ◽  
Michael Carnell ◽  
Chris Brownlee ◽  
...  
Keyword(s):  
Single Cell ◽  
Label Free ◽  
Inertial Microfluidics

scholarly journals Label-free Isolation and Single Cell Biophysical Phenotyping Analysis of Primary Cardiomyocytes Using Inertial Microfluidics

2020 ◽  
Author(s):  
Hossein Tavassoli ◽  
Prunella Rorimpandey ◽  
Young Chan Kang ◽  
Michael Carnell ◽  
Chris Brownlee ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Single Cell ◽  
Real Time ◽  
Cell Types ◽  
Label Free ◽  
Inertial Microfluidics ◽  
And Function ◽  
Deformability Cytometry

AbstractTo advance our understanding of cardiomyocyte identity and function, we need appropriate tools to isolate pure primary cardiomyocytes. We have developed a label-free method to purify viable cardiomyocytes from mouse neonatal hearts using a simple inertial microfluidics biochip. Cardiomyocytes were sorted from neonatal hearts and isolated to >90% purity and their physico-mechanical properties were evaluated using real time deformability cytometry. Purified cardiomyocytes were viable and retained their identity and function as depicted by expression of cardiac specific markers and contractility. Furthermore, we showed that cardiomyocytes have a distinct physico-mechanical phenotype that could be used as an intrinsic biophysical marker to distinguish these cells from other cell types within the heart. Taken together, this cardiomyocyte isolation and phenotyping method could serve as a valuable tool to progress our understanding of cardiomyocyte identity and function, which will ultimately benefit many diagnostic development and cardiac treatment studies.

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