Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

10.3791/59043 ◽  
2019 ◽  
Author(s):  
Anja Schulz-Kuhnt ◽  
Sebastian Zundler ◽  
Anika Grüneboom ◽  
Clemens Neufert ◽  
Stefan Wirtz ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Allergic Inflammation ◽  
Light Sheet ◽  
In Vivo Model ◽  
Advanced Imaging ◽  
Light Sheet Microscopy

scholarly journals Spatiotemporal co-dependency between macrophages and exhausted CD8+ T cells in cancer

2021 ◽  
Author(s):  
Kelly Kersten ◽  
Kenneth H Hu ◽  
Alexis J Combes ◽  
Bushra Samad ◽  
Tory Harwin ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Light Sheet ◽  
Light Sheet Microscopy ◽  
Spatially Resolved ◽  
Hypoxic Conditions ◽  
Synaptic Interactions ◽  
Positive Feedback Circuit ◽  
Major Impediment

T cell exhaustion is a major impediment to anti-tumor immunity. However, it remains elusive how other immune cells in the tumor microenvironment (TME) contribute to this dysfunctional state. Here we show that the biology of tumor-associated macrophages (TAM) and exhausted T cells (Tex) in the TME is extensively linked. We demonstrate that in vivo depletion of TAM reduces exhaustion programs in tumor-infiltrating CD8+ T cells and reinvigorates their effector potential. Reciprocally, transcriptional and epigenetic profiling reveals that Tex express factors that actively recruit monocytes to the TME and shape their differentiation. Using lattice light sheet microscopy, we show that TAM and CD8+ T cells engage in unique long-lasting antigen-specific synaptic interactions that fail to activate T cells, but prime them for exhaustion, which is then accelerated in hypoxic conditions. Spatially resolved sequencing supports a spatiotemporal self-enforcing positive feedback circuit that is aligned to protect rather than destroy a tumor.

Multiphoton Light-sheet Microscopy at Optimal Pulse Frequency for Fast In Vivo Imaging

2020 ◽  
Author(s):  
Vincent Maioli ◽  
Antoine Boniface ◽  
Pierre Mahou ◽  
Júlia Ferrer Ortas ◽  
Lamiae Abdeladim ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
Pulse Frequency ◽  
Light Sheet Microscopy

scholarly journals Coupling optogenetics and light-sheet microscopy to study signal transduction in vivo

2017 ◽  
Vol 145 ◽  
pp. S70-S71
Author(s):  
Prameet Kaur ◽  
Timothy E. Saunders ◽  
Nicholas Tolwinski
Keyword(s):  
Signal Transduction ◽  
Light Sheet ◽  
Light Sheet Microscopy

Functional in vivo imaging using fluorescence lifetime light sheet microscopy (Conference Presentation)

2019 ◽  
Author(s):  
Simon M. Ameer-Beg ◽  
Claire A. Mitchell ◽  
Simon P. Poland ◽  
Robert D. Knight ◽  
Guoqing Wang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

In vivo light‐sheet microscopy resolves localisation patterns of FSD1 , a superoxide dismutase with function in root development and osmoprotection

2020 ◽  
Vol 44 (1) ◽  
pp. 68-87 ◽  
Author(s):  
Petr Dvořák ◽  
Yuliya Krasylenko ◽  
Miroslav Ovečka ◽  
Jasim Basheer ◽  
Veronika Zapletalová ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Root Development ◽  
Light Sheet ◽  
Light Sheet Microscopy

scholarly journals Observing the Cell in Its Native State: Imaging Subcellular Dynamics in Multicellular Organisms

2018 ◽  
Author(s):  
Tsung-Li Liu ◽  
Srigokul Upadhyayula ◽  
Daniel E. Milkie ◽  
Ved Singh ◽  
Kai Wang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
High Speed ◽  
Developmental Stages ◽  
Phenotypic Diversity ◽  
Metastatic Cancer ◽  
Light Sheet ◽  
Intrinsic Variability ◽  
Spatiotemporal Resolution ◽  
Light Sheet Microscopy

AbstractTrue physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution without inducing undue stress on either. We combined lattice light sheet microscopy with two-channel adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages, and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.One Sentence SummaryCombining lattice light sheet microscopy with adaptive optics enables high speed, high resolution in vivo 3D imaging of dynamic processes inside cells under physiological conditions within their parent organisms.

scholarly journals Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy

ACS Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 1036-1049 ◽  
Author(s):  
Guy Malkinson ◽  
Pierre Mahou ◽  
Élodie Chaudan ◽  
Thierry Gacoin ◽  
Ali Y. Sonay ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy

Advances in imaging feto-placental vasculature: new tools to elucidate the early life origins of health and disease

2020 ◽  
pp. 1-11
Author(s):  
Yutthapong Tongpob ◽  
Caitlin Wyrwoll
Keyword(s):  
Ex Vivo ◽  
Light Sheet ◽  
Experimental Models ◽  
Cardiovascular Development ◽  
Micro Computed Tomography ◽  
Placental Function ◽  
Light Sheet Microscopy ◽  
Health And Disease ◽  
Insight Into

Abstract Optimal placental function is critical for fetal development, and therefore a crucial consideration for understanding the developmental origins of health and disease (DOHaD). The structure of the fetal side of the placental vasculature is an important determinant of fetal growth and cardiovascular development. There are several imaging modalities for assessing feto-placental structure including stereology, electron microscopy, confocal microscopy, micro-computed tomography, light-sheet microscopy, ultrasonography and magnetic resonance imaging. In this review, we present current methodologies for imaging feto-placental vasculature morphology ex vivo and in vivo in human and experimental models, their advantages and limitations and how these provide insight into placental function and fetal outcomes. These imaging approaches add important perspective to our understanding of placental biology and have potential to be new tools to elucidate a deeper understanding of DOHaD.

scholarly journals Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish

2015 ◽  
Vol 6 (5) ◽  
pp. 1797 ◽  
Author(s):  
Zhe Yang ◽  
Li Mei ◽  
Fei Xia ◽  
Qingming Luo ◽  
Ling Fu ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Light Sheet Microscopy

scholarly journals Functional in vivo imaging using fluorescence lifetime light-sheet microscopy

2017 ◽  
Vol 42 (7) ◽  
pp. 1269 ◽  
Author(s):  
Claire A. Mitchell ◽  
Simon P. Poland ◽  
James Seyforth ◽  
Jakub Nedbal ◽  
Thomas Gelot ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
In Vivo Imaging ◽  
Light Sheet ◽  
Light Sheet Microscopy
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