Development of a Mobile Mitochondrial Physiology Laboratory for Measuring Mitochondrial Energetics in the Field

Author(s):  
Hailey A. Parry ◽  
Kang Nian Yap ◽  
Geoffrey E. Hill ◽  
Wendy R. Hood ◽  
L. Bruce Gladden ◽  
...  

HAPS Educator ◽  
2017 ◽  
Vol 21 (3) ◽  
pp. 48-59
Author(s):  
Michael Pergola ◽  
Carol Britson ◽  
Audra Schaefer


2014 ◽  
Vol 19 (6) ◽  
pp. 34-40 ◽  
Author(s):  
Stephanie J. Guzzo ◽  
Susan W. Yeargin ◽  
Jeffery S. Carr ◽  
Timothy J. Demchak ◽  
Jeffrey E. Edwards

Context:Many athletic trainers use “ice to go” to treat their athletes. However, researchers have reported that icing a working muscle may negate intramuscular (IM) cooling.Objective:The purpose of our study was to determine the length of time needed to cool the gastrocnemius while walking followed by rest.Design:A randomized crossover study design was used.Setting:Exercise Physiology Laboratory.Patients or Other Participants:Nine healthy, physically active males and females (males 5, females 4; age 24.0 ± 2.0 years; height 174.0 ± 8.0 cm; weight 86.3 ± 6.5 kg; skinfold taken at center of gastrocnemius greatest girth, R leg 20.3 ± 4.4 mm, L leg 19.6 ± 4.1 mm) without lower extremity injury or cold allergy volunteered to complete the study.Intervention:Participants randomly experienced three treatment conditions on separate days: rest (R), walk for 15 minutes followed by rest (W15R), or walk for 30 minutes followed by rest (W30R). During each treatment, participants wore a 1 kg ice bag secured to their right gastrocnemius muscle. Participants walked at a 4.5km/hr pace on a treadmill during the W15R and W30R trials.Main Outcome Measures:A 1 × 3 within groups ANOVA was used to determine the effect of activity on cooling time needed for the gastrocnemius temperature to decrease 6 °C below baseline.Results:The R condition cooled faster (25.9 ± 5.5 min) than both W15R (33.7 ± 9.3 min;P= .002) and W30R (49.4 ± 8.4 min;P< .001). Average time to decrease 6 °C after W15R was 18.7 ± 9.3 minutes and after W30R was 19.4 ± 8.4 minutes.Conclusions:Clinicians should instruct their patients to stay and ice or to keep the ice on for an additional 20 minutes after they stop walking and begin to rest.



1996 ◽  
Vol 271 (6) ◽  
pp. S48 ◽  
Author(s):  
M S Jensen

Physiology educators read journals, such as this one, to gather ideas about curriculum and instruction. Most articles focus on curriculum (i.e., what is taught), but this paper will focus on instruction (i.e., how curriculum is implemented). Just as there are different types of curricula, there are different types of instruction. The most common strategy is lecture. Lectures are extremely efficient for delivering large amounts of information in a short period of time. A common laboratory strategy is discovery or inquiry-based learning (i.e., giving students tools, cognitive and physical, to deduce new information via investigations). A third instructional strategy is the use of cooperative learning. Proper conditions are required for each instructional strategy, and problems arise when the wrong combinations are put together. This paper will describe how a cooperative learning environment can be created in the anatomy and physiology laboratory through the use of cooperative quizzes. It will include a brief introduction to the pedagogical theory behind cooperative learning and an evaluation of the effectiveness of quizzes compared with more traditional methods.



2018 ◽  
Vol 17 (2) ◽  
pp. 181
Author(s):  
Latifa Fekri ◽  
Ridwan Affandi ◽  
Muhammad Fajar Rahardjo ◽  
Tatag Budiardi ◽  
Charles Parningotan Haratua Simanjuntak ◽  
...  

<p align="center"><strong>ABSTRACT</strong></p><p align="center"> </p><p>This study aimed to analyze the effect of water temperature on the physiological condition and growth performance of freshwater eel elver<em> Anguilla bicolor bicolor </em>(McClelland, 1844). This study was conducted in March 2017 at the Physiology Laboratory of Aquatic Animal, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University. The study used a completely randomized design with five different levels of temperature (22<sup>°</sup>C, 24<sup>°</sup>C, 26<sup>°</sup>C, 28<sup>°</sup>C, and 30<sup>°</sup>C) as treatments with two replications. The size of elver was 2‒3 g. Fish were fed with 1 mm pellet containing 45% of protein. The feeding level was 7 % of fish biomass and the feeding frequency was two times a day. The results showed that temperatures range from 24‒30<sup>°</sup>C could be used for freshwater eel elver rearing and 28‒30<sup>°</sup>Cwere the best temperatures to support survival and growth performance of eel elver. A temperature of 24<sup>°</sup>C was the best temperature that could reduce the metabolism rate and did not cause stress on the elver.</p><p> </p><p>Keywords: elver, physiological conditions, growth performance, metabolism, temperature</p><p> </p><p> </p><p align="center"><strong>ABSTRAK</strong></p><p align="center"><strong> </strong></p><p>Penelitian dengan tujuan menganalisis pengaruh suhu terhadap kondisi fisiologis dan kinerja pertumbuhan <em>elver</em> ikan sidat (<em>Anguilla bicolor bicolor</em> McClelland, 1844) telah dilakukan pada bulan Maret 2017 di Laboratorium Fisiologi Hewan Air FPIK IPB. Penelitian menggunakan rancangan acak lengkap dengan lima perlakuan suhu berbeda (22<sup>°</sup>C, 24<sup>°</sup>C, 26<sup>°</sup>C, 28<sup>°</sup>C, dan 30<sup>°</sup>C) dengan masing-masing dua ulangan. Ukuran benih yang digunakan 2‒3 g. Pakan yang diberikan berupa pellet berukuran 1 mm dengan kadar protein 45%. Jumlah pakan yang diberikan (FR) adalah 7% dari biomassa ikan dan diberikan dua kali sehari. Hasil penelitian menunjukkan bahwa kisaran suhu 24‒30<sup>°</sup>C dapat digunakan dalam pemeliharaan <em>elver</em> ikan sidat, dan suhu 28‒30<sup>°</sup>C merupakan suhu yang sangat baik untuk mendukung kelangsungan hidup dan pertumbuhan <em>elver</em> ikan sidat. Suhu media 24<sup>°</sup>C adalah suhu terbaik yang dapat menekan laju metabolisme dengan tidak menyebabkan stres pada <em>elver </em>ikan sidat.</p><p> </p><p>Kata kunci: <em>elver</em>, kondisi fisiologis, kinerja pertumbuhan, metabolisme, suhu</p><p> </p><p> </p>



2018 ◽  
Vol 38 (3) ◽  
pp. 158-163
Author(s):  
Komang Sri Rahayu Widiasari ◽  
Susanthy Djajalaksana ◽  
Harun Al Rasyid

Background: Muscle wasting is one of extrapulmonary manifestations that occur in 20-40% of patients with COPD as a result of an imbalance of protein synthesis and degradation, where it is thought to be a consequence of chronic inflammation. One of the factor that affect muscle wasting is nutritional factor. The purpose of this study is to prove that nutrition therapy can improve inflammation (measured by levels of leptin, adiponectin) further improve muscle wasting and improve the quality of life of patients COPD with muscle wasting. Method: The clinical study design is pre and post auto control quasi experimental in stable COPD patients with comorbid muscle wasting. The experiment was conducted in Pulmonary Outpatient Clinic Dr. Saiful Anwar Hospital and Physiology Laboratory of Medical Faculty Brawijaya University. Chronic obstructive pulmonary disease was diagnosed based on 2014 GOLD criteria. Muscle wasting was diagnosed through examination of the BIA. Levels of leptin and adiponectin was measured using ELISA method, and quality of life was assessed using CAT score. We measured BIA, Leptin, Adiponectin and CAT in 32 COPD patients with muscle wasting, before and after 12 weeks supplementation of Opiocephalus striatus extract 3x1000mg/day. Results: There were significant increased of BMI (p = 0.046), no significant increase of FFMI (p = 0506), a significant decrease in leptin levels (p = 0.000) and a significant increase in adiponectin levels (p = 0.048) and improvement of quality of life (score CAT) (p = 0.000) ) after administration of opiocephalus striatus extract for 12 weeks. Conclusion: Suplementation of Opiocephalus striatus extract for 12 weeks can improve BMI, decrease levels of leptin and increase level of adiponectin resulting in improvement of quality of life in stable COPD patients with muscle wasting.





1992 ◽  
Vol 262 (6) ◽  
pp. S25
Author(s):  
R G Montes ◽  
R F Gottal ◽  
T M Bayless ◽  
T R Hendrix ◽  
J A Perman

Breath hydrogen testing (BHT) is a simple and reliable method for identifying impaired carbohydrate absorption. We describe a laboratory exercise in physiology for medical students using BHT as the teaching tool. The students collect fasting samples of expired air from each other using a simple nasal prong technique. They then drink one of several different aqueous carbohydrate solutions. Additional samples of expired air are collected by the students at 90 and 120 min after substrate ingestion and are analyzed by gas chromatography. Between sampling periods, discussions of digestive physiology are provided by the faculty. Students tabulate their BHT results as well as recording any symptoms using a standard scoring system. A total of 460 students have participated. We found that the percentage of students who malabsorbed a given substrate was similar each year. The results obtained in these student exercises closely parallel those reported in the literature. We conclude that BHT is an excellent teaching tool for illustrating carbohydrate digestion and absorption, even when performed by minimally trained subjects.



2015 ◽  
Vol 39 (4) ◽  
pp. 405-410 ◽  
Author(s):  
Caroline Altermann ◽  
Rithiele Gonçalves ◽  
Marcus Vinícius S. Lara ◽  
Ben-Hur S. Neves ◽  
Pâmela B. Mello-Carpes

The purpose of the present article is to describe three simple practical experiments that aim to observe and discuss the anatomic and physiological functions and differences between arteries and veins as well as the alterations observed in skin blood flow in different situations. For this activity, students were divided in small groups. In each group, a volunteer is recruited for each experiment. The experiments only require a sphygmomanometer, rubber bands, and a clock and allow students to develop a hypothesis to explain the different responses to the interruption of arterial and venous blood flow. At the end, students prepare a short report, and the results are discussed. This activity allows students to perceive the presence of physiology in their daily lives and helps them to understand the concepts related to the cardiovascular system and hemodynamics.



1942 ◽  
Vol 42 (5) ◽  
pp. 610
Author(s):  
Hazel Houston ◽  
Edmond J. Farris


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