Study on the properties of denitrifying carbon sources from cellulose plants and their nitrogen removal mechanisms

Carbon sources of cellulose plants are the promising materials that enhancing the activities of denitrifying bacteria in the groundwater system. To further verify the denitrification performance of cellulose plants and the main factors of affecting the denitrifying system, six cellulose plants from agricultural wastes (wood chip, corn cob, rice husk, corn straw, wheat straw, and sugar cane) were selected for bioavailable organic matter leaching experiments, carbon denitrification experiments, functional bacteria identification, and analysis experiments. The results show that the extracts of cellulose plants contain a mixed carbon sources system including small molecular organic acids, sugars, nitrogen-containing organic components, and esters. The qPCR results showed that the denitrifying bacteria had obvious advantages compare to anaerobic ammonia-oxidizing bacteria during the stable period; the denitrification experiment showed that each of six cellulose plants removed more than 80% of nitrogen, and the denitrification rates reached 1.00–2.00 mg N cm−3·d−1. The supplement of cellulose plants promotes the metabolism rate of denitrifying bacteria, and the additional denitrifying bacteria have little effect on nitrate removal. In summary, the expected denitrification reaction occurred in the cellulose plant system, which is suitable as a carbon source material for water body nitrogen pollution remediation.

Liver Disease Prediction Using Machine Learning Classification

People have disorder of liver that require medical care at correct time. It is utmost important to find the disease before it elapse the curable stage. Significantly, much of understanding of organ development has arisen from analyses of patients with liver deficiencies. Data mining is beneficial to find the disease at early stage based on the factors that can be gathered by performing test on the patient. Nowadays, around 65 % of the population in India are eating junk foods which minimize the metabolism rate and effect liver in many ways. In recent years, liver disorders have excessively increased and are still considered to be life threatening because it has caused low survivability. Still the patients having liver diseases are increasing and the symptoms of the diseases are difficult to identify. The doctors often failed to identify the symptoms which can cause severe damages to the patient and it requires utmost attention. So, we are applying Medical Data Mining (MDM) for predicting the liver disease by using the historical data and understanding their patterns. Here we are using prediction model i.e. Support Vector Machine (SVM) to achieve the goal.

Respons fisiologis benur udang vaname (Litopenaeus vannamei) terhadap penambahan kalsium selama adaptasi di salinitas rendah

The purpose of this study was to determine the optimum addition of calcium during the adaptation period of white shrimp seed in low salinity media, so the physiological conditions of shrimp still support maximally survival and growth. The research was carried out by using the Completely Randomized Design, i.e., the addition of calcium in freshwater for decreasing water salinity from 20 g L-1 to 0.5 g L-1 as much as 150, 200, 250, 300, and 350 mg L-1, respectively. The freshwater used in the salinity reduction process was based on previous research that included sodium and potassium additions of 75 mg L-1 and 50 mg L-1, respectively. The test animal was a white shrimp stadium PL15 that had been acclimated for 5 days to a medium salinity of 20 g L-1. The results of this study showed that the addition of 300 mg L-1- calcium in freshwater during the adaptation for 96 hours significantly accelerate the achievement of molting time (1360 minutes), suppress the stress levels and metabolism rate (body fluid glucose of 169.80 mg L-1 and oxygen consumption level of 0.95 mg O2 g-1 h-1), and produce the highest survival rate of white shrimp seed (99%).Keywords: adaptation, calcium, low salinity, physiology, white shrimp

Role of Tacrolimus C/D Ratio in the First Year After Pediatric Liver Transplantation

Background: The calcineurin inhibitor (CNI) tacrolimus (TAC) is a cornerstone agent in immunosuppressive therapy in pediatric liver transplantation (LTX). Adverse effects limit the use of CNI. In adults, calculating the individual TAC metabolism rate allows to estimate the transplant recipient's risk for therapy-associated complications.Methods: A retrospective, descriptive data analysis was performed in children who had undergone LTX in 2009–2017 and had received TAC twice daily in the first year after LTX. A weight-adjusted concentration/dose ratio (C/D ratio) was calculated [TAC trough level/(daily TAC dose/body weight)] every 3 months after LTX to estimate the average individual TAC metabolism rate. Depending on the C/D ratio, all patients were divided into two groups: fast metabolizers (FM) and slow metabolizers (SM). Clinical and laboratory parameters were analyzed as risk factors in both groups.Results: A total of 78 children (w 34, m 44, median age at LTX 2.4; 0.4–17.0 years) were enrolled in the study. FM (SM) had a mean C/D ratio of <51.83 (≥51.83) ng/ml/(mg/kg). FM were younger at the time of LTX (median age 1.7; 0.4–15.8 years) than SM (5.1, 0.4–17.0), p = 0.008. FM were more likely to have biliary atresia (20/39, 51%) compared to SM (11/39, 28%), p = 0.038, whereas SM were more likely to have progressive familial intrahepatic cholestasis (9/39, 23%) vs. in FM (1/39, 3%), p = 0.014. Epstein–Barr virus (EBV) infection occurred more frequently in FM (27/39, 69%) than SM (13/39, 33%), p = 0.002. Three FM developed post-transplant lymphoproliferative disorder. The annual change of renal function did not differ in both groups (slope FM 1.2 ± 0.6; SM 1.4 ± 0.8 ml/min/1.73 m2 per year, and p = 0.841).Conclusions: Calculation of individual, weight-adjusted TAC C/D ratio is a simple, effective, and cost-efficient tool for physicians to estimate the risk of therapy-associated complications and to initiate individual preventive adjustments after pediatric LTX. Lower TAC levels are tolerable in FM, especially in the presence of EBV infection, reduced renal function, or when receiving a liver transplant in the first 2 years of life.

Parameters sensitivity assessment and heat source localization using infrared imaging techniques

Background At present, infrared (IR) imaging is used both as a non-invasive and a non-ionizing technology. Using an IR camera, it is possible to measure body surface temperature in order to detect tumors and malignant cells. Tumors have a high amount of vasculature and an enhanced metabolism rate, which may result in an increase in body surface temperature by several degrees above its normal level.

Parameters sensitivity assessment and heat source localization using infrared imaging techniques

Background At present, infrared (IR) imaging is used both as a non-invasive and a non-ionizing technology. Using an IR camera, it is possible to measure body surface temperature in order to detect tumors and malignant cells. Tumors have a high amount of vasculature and an enhanced metabolism rate, which may result in an increase in body surface temperature by several degrees above its normal level.

Label-free spectral imaging to study drug distribution and metabolism in single living cells

During drug development, evaluation of drug and its metabolite is an essential process to understand drug activity, stability, toxicity and distribution. Liquid chromatography (LC) coupled with mass spectrometry (MS) has become the standard analytical tool for screening and identifying drug metabolites. Unlike LC/MS approach requiring liquifying the biological samples, we showed that spectral imaging (or spectral microscopy) could provide high-resolution images of doxorubicin (dox) and its metabolite doxorubicinol (dox’ol) in single living cells. Using this new method, we performed measurements without destroying the biological samples. We calculated the rate constant of dox translocating from extracellular moiety into the cell and the metabolism rate of dox to dox’ol in living cells. The translocation rate of dox into a single cell for spectral microscopy and LC/MS approaches was similar (~ 1.5 pM min−1 cell−1). When compared to spectral microscopy, the metabolism rate of dox was underestimated for about every 500 cells using LC/MS. The microscopy approach further showed that dox and dox’ol translocated to the nucleus at different rates of 0.8 and 0.3 pM min−1, respectively. LC/MS is not a practical approach to determine drug translocation from cytosol to nucleus. Using various methods, we confirmed that when combined with a high-resolution imaging, spectral characteristics of a molecule could be used as a powerful approach to analyze drug metabolism. We propose that spectral microscopy is a new method to study drug localization, translocation, transformation and identification with a resolution at a single cell level, while LC/MS is more appropriate for drug screening at an organ or tissue level.

13C-METHACETIN BREATHE TEST IN EARLY DIAGNOSTICS OF NON-ALCOHOLIC FATTY LIVER DISEASE

The aim: Of the study was to evaluate the early utility changes of the 13C methacetin breath test parameters in patients with NAFLD. Materials and methods: There were included 50 subjects in the study, among them 35 patients had steatosis and 15 patients had steatohepatitis, including 35 (70.0%) male subjects and 15 (30.0%) female subjects. The control group included 17 apparently healthy volunteers, among them 10 (58.8%) subjects were male and 7 (41.2%) subjects were female. Results: It was determined that metabolism kinetics in case of liver steatosis was significant decreased more than 30% compared to the control group (p = 0.0001) and in case of steatohepatitis that decrease was more than 65% (p = 0.00001) compared with normal values. It resulted in less cumulative dose accumulation in steatosis (p = 0.00001) and steatohepatitis (p = 0.00001). Among the reasons for the decrease in the kinetics of metabolism in steatosis, there were insufficient response of hepatocytes on 10 minutes (<10 dose/h,%) in 40% of cases and reduction of metabolism rate amplitude at 20-40 minutes following methacetin administration. Conclusions: The results of 13C-methacetin breath test demonstrate that in patients with NAFLD there is a gradual slowing of metabolism rate in hepatocytes, which leads to a decrease in cumulative dose.