Association Mapping Reveals Novel Stem Rust Resistance Loci in Durum Wheat at the Seedling Stage

Stem rust resistance gene Sr13, found frequently in tetraploid wheats, was tested effective against Puccinia graminis f. sp. tritici pathotype Ug99 (TTKSK) and its derivatives. It remains a candidate for developing new cultivars with diverse combinations of stem rust resistance genes. To combine Sr13 with other genes that produce a similar phenotype, linked markers would be required. We used the AFLP approach to identify markers linked closely with Sr13. The STS marker AFSr13, derived from an AFLP fragment, mapped at 3.4–6.0 cM proximal to Sr13 across three mapping populations. Marker dupw167, previously reported to be linked with Sr13, mapped 2.3–5.7 cM distal to Sr13 in four F3 populations. Marker gwm427 mapped proximal to AFSr13 in two populations, and these markers were monomorphic on one population each. The map order dupw167–Sr13–AFSr13–gwm427 was deduced from the recombination data. Markers dupw167 and AFSr13 were validated on 21 durum wheat genotypes. Combination of dupw167 and AFSr13 would facilitate marker-assisted selection of Sr13 in segregating populations. At the hexaploid level, only gwm427 showed polymorphism and differentiated the presence of Sr13 in 10 of the 15 backcross derivatives carrying Sr13 from their Sr13-lacking recurrent parents.

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THE INHERITANCE OF REACTION TO BLACK STEM RUST OF WHEAT IN A DICOCCUM × VULGARE CROSS

Canadian Journal of Research ◽

10.1139/cjr29-010 ◽

1929 ◽

Vol 1 (2) ◽

pp. 163-188 ◽

Cited By ~ 4

Author(s):

J. B. Harrington ◽

W. K. Smith

Keyword(s):

Plant Breeding ◽

Stem Rust ◽

Rust Resistance ◽

Genetic Factor ◽

Stem Rust Resistance ◽

Seedling Stage ◽

Genetical Study ◽

Seed Shape ◽

Seedling Resistance ◽

Black Stem Rust

A genetical study of resistance of wheat to black stem rust, and a plant breeding attack on the rust problem are described. A large F2 population of the cross Vernal (T. dicoccum) × Marquis (T. vulgare) was grown under severe natural epidemic conditions in the field and hundreds of F3 progenies were exposed in the seedling stage, under controlled conditions, to pure physiologic forms of rust. In the field Vernal is highly resistant and Marquis susceptible to most forms of stem rust. Resistance in the field proved incompletely dominant and appeared to be governed by a single genetic factor. Marquis and Vernal were found to differ by one main genetic factor, Rb, for seedling reaction to form 21. This factor Rb, carried by Vernal, also governs seedling resistance to forms 17, 29 and 36 and appears to be responsible for the slight seedling resistance of Vernal to form 27. There was some evidence that the factor Rb is the same factor that controls the resistance of the F2 plants to the forms of rust in the field (forms 17, 21, 29 and 36 were known to be present.) A different factor Ra causes the resistance of Marquis seedlings to form 27. Vernal resistance was not found to be associated closely with the seed shape of that variety nor with its adherence of glumes to the seed.

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Characterization and Inheritance of Adult Plant Stem Rust Resistance in Durum Wheat

Crop Science ◽

10.2135/cropsci1997.0011183x003700040010x ◽

1997 ◽

Vol 37 (4) ◽

pp. 1094-1098 ◽

Cited By ~ 11

Author(s):

R. A. Hare

Keyword(s):

Durum Wheat ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Adult Plant ◽

Plant Stem

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Association mapping and gene–gene interaction for stem rust resistance in CIMMYT spring wheat germplasm

Theoretical and Applied Genetics ◽

10.1007/s00122-011-1664-y ◽

2011 ◽

Vol 123 (8) ◽

pp. 1257-1268 ◽

Cited By ~ 91

Author(s):

Long-Xi Yu ◽

Aaron Lorenz ◽

Jessica Rutkoski ◽

Ravi P. Singh ◽

Sridhar Bhavani ◽

...

Keyword(s):

Association Mapping ◽

Spring Wheat ◽

Stem Rust ◽

Rust Resistance ◽

Gene Interaction ◽

Stem Rust Resistance ◽

Wheat Germplasm

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Genome-Wide Association Mapping of Stem Rust Resistance inHordeum vulgaresubsp.spontaneum

G3 Genes|Genome|Genetics ◽

10.1534/g3.117.300222 ◽

2017 ◽

Vol 7 (10) ◽

pp. 3491-3507 ◽

Cited By ~ 13

Author(s):

Ahmad H. Sallam ◽

Priyanka Tyagi ◽

Gina Brown-Guedira ◽

Gary J. Muehlbauer ◽

Alex Hulse ◽

...

Keyword(s):

Association Mapping ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Genome Wide Association ◽

Genome Wide

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Mapping and Characterization of a Wheat Stem Rust Resistance Gene in Durum Wheat “Kronos”

Frontiers in Plant Science ◽

10.3389/fpls.2021.751398 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hongna Li ◽

Lei Hua ◽

Matthew N. Rouse ◽

Tianya Li ◽

Shuyong Pang ◽

...

Keyword(s):

Durum Wheat ◽

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Wheat Variety ◽

Wheat Breeding ◽

Stem Rust Resistance ◽

Wheat Stem Rust ◽

Seedling Resistance ◽

Or Gene

Wheat stem (or black) rust is one of the most devastating fungal diseases, threatening global wheat production. Identification, mapping, and deployment of effective resistance genes are critical to addressing this challenge. In this study, we mapped and characterized one stem rust resistance (Sr) gene from the tetraploid durum wheat variety Kronos (temporary designation SrKN). This gene was mapped on the long arm of chromosome 2B and confers resistance to multiple virulent Pgt races, such as TRTTF and BCCBC. Using a large mapping population (3,366 gametes), we mapped SrKN within a 0.29 cM region flanked by the sequenced-based markers pku4856F2R2 and pku4917F3R3, which corresponds to 5.6- and 7.2-Mb regions in the Svevo and Chinese Spring reference genomes, respectively. Both regions include a cluster of nucleotide binding leucine-repeat (NLR) genes that likely includes the candidate gene. An allelism test failed to detect recombination between SrKN and the previously mapped Sr9e gene. This result, together with the similar seedling resistance responses and resistance profiles, suggested that SrKN and Sr9e may represent the same gene. We introgressed SrKN into common wheat and developed completely linked markers to accelerate its deployment in the wheat breeding programs. SrKN can be a valuable component of transgenic cassettes or gene pyramids that includes multiple resistance genes to control this devastating disease.

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