scholarly journals In vitro antimicrobial susceptibility patterns of Propionibacterium acnes isolated from patients with acne vulgaris

2016 ◽  
Vol 10 (10) ◽  
pp. 1140-1145 ◽  
Author(s):  
Indu Biswal ◽  
Rajni Gaind ◽  
Neeraj Kumar ◽  
Srujana Mohanty ◽  
Vikas Manchanda ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Propionibacterium Acnes ◽  
Clinical Laboratory ◽  
Acne Vulgaris ◽  
Brain Heart Infusion ◽  
Tetracycline Resistance ◽  
Agar Dilution ◽  
Resistance Rates ◽  
Systemic Antibiotics

Introduction: Propionibacterium acnes has been implicated in the development of acne vulgaris. Rampant use of topical and systemic antibiotics for acne vulgaris has led to resistance due to selective pressure. This study aimed to determine antibiotic resistance of P. acnes. Methodology: A total of 102 samples were collected from acne lesions and cultured onto sheep’s blood agar and brain-heart infusion agar supplemented with 5 g/L glucose and 2 mg/L furazolidone) (BHIg) under aerobic and anaerobic conditions. Species identification was done by conventional methods and the VITEK2 Compact system. The isolates were tested for penicillin, erythromycin, clindamycin, ciprofloxacin, nadifloxacin, and tetracycline by E-test, and minimum inhibitory concentration (MIC) of minocycline was determined by agar dilution on BHIg. MIC results were interpreted as per EUCAST (European Committee on Antimicrobial Susceptibility Testing) and CLSI (Clinical Laboratory Standards Institute) guidelines. Results: P. acnes was the most common anaerobe (66%) isolated. Resistance rates using EUCAST and CLSI breakpoints were 10.6% and 6.1%, 7.6% and 0%, 7.8% and 0% for erythromycin, clindamycin, and minocycline, respectively. Tetracycline resistance was observed in 9.2% isolates irrespective of the interpretative criteria used. MIC50 and MIC90 values for nadifloxacin (0.25 and 1 µg/mL) were found to be twofold lower than those for ciprofloxacin (0.5 and 1 µg/mL). Similarly, MIC50 and MIC90 values for minocycline (0.125 and 0.5 µg/mL) were also two- to threefold lower than those for tetracycline (0.38 and 1 µg/mL). Conclusions: To the best of our knowledge, this is the first study focusing on P. acnes resistance from India.

scholarly journals Association of Antimicrobial Susceptibility and Treatment Outcome in Acne Vulgaris Patients: A Pilot Study

2020 ◽  
Vol 12 (04) ◽  
pp. 233-238
Author(s):  
Ashvini K. Yadav ◽  
Suneel Bhooshan ◽  
Allen Johnson ◽  
Dinesh P. Asati ◽  
Shashwati Nema ◽  
...  
Keyword(s):  
Treatment Outcome ◽  
Antimicrobial Susceptibility ◽  
Acne Vulgaris ◽  
Tertiary Care ◽  
Care Hospital ◽  
In Vitro Susceptibility ◽  
Persistent Infections ◽  
Significant Difference ◽  
Cutibacterium Acnes

Abstract Purpose Cutibacterium acnes (C. acnes) is an emerging pathogen that is highly resistant to antibiotics and is capable of causing persistent infections that are difficult to treat. Methods & Materials Acne vulgaris patients visiting dermatology OPD of our tertiary care hospital during the study period of 2 months were recruited. Skin swabs were collected, and the sample was processed on 5% sheep-blood agar for anaerobic culture by the GasPak method. Isolates were identified by the standard biochemical test. Antimicrobial susceptibility testing was performed for clinically relevant antibiotics by the E-strip method. The clinical response was evaluated after 1-month follow-up to the prescribed antibiotics. Results Minocycline, doxycycline, ceftriaxone, and tetracycline were the most effective antibiotics. Nonsusceptibility to clindamycin and erythromycin were observed in 11.9% and 31% isolates, respectively, with 9.5% isolates being nonsusceptible to both. For none of the antibiotics we found significant difference in the proportion of susceptible and nonsusceptible isolates between mild, moderate, and severe grades of acne vulgaris. For none of the antibiotic regimens, significant difference was observed between nonresponders and responders. Twenty-seven patients received clindamycin and among them 16 of 19 responders and 6 of 8 nonresponders yielded growth of clindamycin-susceptible isolates (p = 0.57). Conclusion We observed significant prevalence of resistant strains of C. acnes among patients with acne vulgaris. No association was observed between in vitro susceptibility results and treatment outcome.

scholarly journals In vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.

1996 ◽  
Vol 40 (9) ◽  
pp. 2232-2235 ◽  
Author(s):  
H M Wexler ◽  
E Molitoris ◽  
D Molitoris ◽  
S M Finegold
Keyword(s):  
Antimicrobial Activity ◽  
Clinical Laboratory ◽  
Anaerobic Bacteria ◽  
Bacteroides Fragilis ◽  
National Committee ◽  
Dilution Technique ◽  
Agar Dilution ◽  
Group Species ◽  
Clostridium Spp

The antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the National Committee for Clinical Laboratory Standards-approved Wadsworth agar dilution technique. The species tested included Bacteroides fragilis (n = 91), other members of the B. fragilis group (n = 130), Campylobacter gracilis (n = 15), other Bacteroides spp. (n = 16), Prevotella spp. (n = 49), Porphyromonas spp. (n = 15), Fusobacterium spp. (n = 62), Bilophila wadsworthia (n = 24), Sutterella wadsworthensis (n = 21), Clostridium spp. (n = 61), Peptostreptococcus spp. (n = 38), and gram-positive non-spore-forming rods (n = 35). Trovafloxacin inhibited all strains of B. fragilis at < or = 0.5 microgram/ml, 99% of other B. fragilis group species at < or = 2 micrograms/ml, and 96% of all anaerobes tested at < or = 2 micrograms/ml.

scholarly journals Antimicrobial Susceptibility of Porphyromonas Gingivalis Strains Isolated From Periodontal Patients by Three Testing Protocols

2020 ◽  
Author(s):  
Lorena Isbej ◽  
Natacha Oyarzo ◽  
María José Contreras ◽  
Duniel Ortuño ◽  
Marusella Lam ◽  
...  
Keyword(s):  
Laboratory Test ◽  
Porphyromonas Gingivalis ◽  
Antimicrobial Susceptibility ◽  
Randomized Clinical Trials ◽  
Blood Agar ◽  
Agar Dilution ◽  
Susceptibility Tests ◽  
High Level ◽  
Testing Protocols

Abstract Background: The main goal of antimicrobials is to eliminate microorganisms that persists despite mechanical treatment. This is the case of Porphyromonas gingivalis (Pg), frequently isolated in patients with periodontitis. Global antibiotic studies evaluated in randomized clinical trials and in vitro studies have shown mixed results regarding effectiveness and susceptibility, even with different protocols where it is not clear if the laboratory test applied can affect the results. This information is relevant in order to obtain clinical outcomes and prevent antimicrobial resistance for their over-prescription or inadequate choice. The objective of this study was to describe the antimicrobial susceptibility in vitro of Pg to metronidazole, clindamycin, amoxicillin plus clavulanate, moxifloxacin and azithromycin in periodontal patients by three testing protocols.Methods: Microbiological samples were obtained in patients with a diagnosis of generalized moderate or severe periodontitis. They were incubated in anaerobic conditions for up to 7 days, and those morphologically compatible with Pg were isolated and identified by a mass spectrometer (MALDI-TOF MS). The three most frequently protocols for antimicrobials susceptibility tests (Blood agar- McFarland 0.5- Epsilometer test; Brucella blood agar- McFarland 1.0- Epsilometer test; Brucella blood agar- McFarland 0.5-Agar dilution) were applied to the same strain describing their profile and reporting any difference between the tests. The breakpoints considered the guidelines of CLSI and previous publications.Results: 50 patients (25 women, 25 men) with periodontitis between 34-69 years were selected. Finally, 25 Pg positives strains (50%) were recovered for the susceptibility analysis and all of them were highly sensitive to all antibiotics (range 96%-100%). Only one strain was resistant to azithromycin in one protocol, and no differences were found in the susceptibility results between the three tests.Conclusion: The Pg strains were highly susceptible to the five antibiotics evaluated in this population, showed a high level of susceptibility and significant agreement between the three tests applied, therefore the type of laboratory test used had not impacted on clinical interpretation. These findings are positives in terms of susceptibility and would provide several antibiotics treatment alternatives, and its prescription could be the best choice for the patient's specific context.

scholarly journals Determination of Antimicrobial Susceptibility Patterns between Different Strains of Propionibacterium Acnes

2019 ◽  
Vol 4 (06) ◽  
Author(s):  
Yusufu, W. N. ◽  
Suleiman, H. O. ◽  
Akwa, V. Y. ◽  
David, D. L. ◽  
Taiga, A.
Keyword(s):  
Antimicrobial Susceptibility ◽  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Antibiotics Resistance ◽  
No Inhibition ◽  
Zones Of Inhibition ◽  
Weak Activity ◽  
Inhibition Zones ◽  
Different Strains ◽  
Susceptibility Patterns

Propionibacterium acnes (P. acnes) isolated in infections of deep tissues was originally thought to be as a result of improper sterilization of skin and hence contamination of medical processes with this commensal/ normal flora of the skin. P. acnes has latter been identified as the principal agent responsible for the progression of some deep tissue diseases. The antimicrobial susceptibility patterns between different strains of P. acnes was determined. Ten (10) P. acnes clinical isolates of infections were considered, two (2) from acne vulgaris and eight (8) [two (2) per recA types 1A1, 1B, II and III] from lumber herniation tissues. Antibiotic susceptibility of some antibiotics was also determined and resistance to tetracycline was seen in 1 isolate, trimethoprim (1 isolate) and weak activity seen in 5 isolates. Heteroresistance to rifampicin was witnessed among 6 isolates. Upon four days of incubation, no inhibition of the bacterial growth by tetracycline in isolate 1(III). Isolate 17(1B) had no inhibition from trimethoprim. There was low inhibition of P. acnes by this antibiotic in isolates 71(IA1), 82(IB), 55(II), 1 (III) and 64(III). Amoxicillin and rifampicin created greater zones of inhibition among P. acnes isolates. Erythromycin was the next most inhibiting antibiotic with greater inhibition zones. Although Rifampicin had large inhibition zones, heteroresistance was observed among isolates 82(IB), 24(II), I (III), 17(IB), 55(II) and 71(IA1) as colonies were observed growing within the zones after 4 days of incubation. The antibiotics resistance expressed in the study were seen displayed among the lumber disc herniation isolates only with none involving acne vulgaris isolates. This study supports the theory that most P. acnes isolates in deep tissues exhibit higher rate of antibiotic resistance. P. acnes hence might be named the etiological agent of foreign-body infections like infections of indwelling medical devices.

Report: In vitro antimicrobial susceptibility of Propionibacterium acnes isolated from acne patients in northern Mexico

2010 ◽  
Vol 49 (9) ◽  
pp. 1003-1007 ◽  
Author(s):  
Roger González ◽  
Oliverio Welsh ◽  
Jorge Ocampo ◽  
Rosa M. Hinojosa-Robles ◽  
Lucio Vera-Cabrera ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Propionibacterium Acnes ◽  
Northern Mexico

An improved susceptibility test based on Amberlite reveals the potential antilisterial activity of fosfomycin in vitro

2013 ◽  
Vol 59 (4) ◽  
pp. 252-259 ◽  
Author(s):  
Lei Han ◽  
Jin'e Lei ◽  
Shaoshan Han ◽  
Li He ◽  
Chaofeng Ma ◽  
...  
Keyword(s):  
Brain Heart Infusion ◽  
Susceptibility Test ◽  
Host Cells ◽  
Clinical Isolates ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Broth Microdilution Method ◽  
Agar Dilution

Listeria monocytogenes is resistant to fosfomycin in vitro but is susceptible in vivo due to increased expression of positive regulator factor A (PrfA) and its dependent factor, hexose phosphate transporter (Hpt), upon infection of host cells. Amberlite, a polymeric adsorbent resin, could induce PrfA-dependent gene expression and thus, in theory, improve the sensitivity of L. monocytogenes to fosfomycin in vitro. In the current study, an improved susceptibility test based on Amberlite was developed using reference strains. Thirty-five clinical isolates were further examined to verify those preliminary results. Briefly, Amberlite increased in vitro fosfomycin sensitivity of all strains. Optimal Amberlite concentrations, as evaluated through the expression of phospholipase B (PlcB) and Hpt, were 10% and 15% (w/v) in agar media and 3% (w/v) in broth media. Mueller–Hinton (MH) medium, tryptone soya (TS) medium, and brain heart infusion (BHI) medium were used to verify the results in the control strains using agar dilution and broth micro- and macro-dilution methods. Better listerial growth was shown in TS and BHI than in MH. Both broth dilution methods yielded lower minimal inhibitory concentration (MIC) of fosfomycin than the agar dilution method. The MIC of fosfomycin for 35 clinical isolates was 2–32 μg/mL, suggesting improved susceptibility. In conclusion, in vitro sensitivity of L. monocytogenes to fosfomycin was substantially improved in the presence of 3% Amberlite-supplemented TSB or BHIB and the broth microdilution method. This improved method revealed the potential antilisterial activity of fosfomycin in vitro and could facilitate the therapy of listeriosis using fosfomycin.

scholarly journals UJI ANTI BAKTERI EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP ZONA HAMBAT BAKTERI JERAWAT Propionibacterium acnes SECARA IN VITRO

2018 ◽  
Vol 10 (01) ◽  
pp. 10
Author(s):  
Ruhana Afifi ◽  
Euis Erlin ◽  
Jeti Rachmawati
Keyword(s):  
Propionibacterium Acnes ◽  
Acne Vulgaris

Jerawat (acne vulgaris) merupakan penyakit kulit yang terjadi karena adanya penyumbatan folikel oleh sel-sel mati, sebum, dan peradangan yang disebabkan oleh� bakteri Propionibacterium acnes pada folikel sebasea. Antibiotik dapat mengobati jerawat namun dapat menimbulkan resistensi dari suatu bakteri, sehingga diperlukan cara yang lebih aman dan lebih murah. Salah satunya dengan penggunaan daun Belimbing wuluh (Averrhoa bilimbi L) karena mengandung zat aktif Flavonoid dan Tanin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan konsentrasi ekstrak daun belimbing wuluh terhadap zona hambat bakteri P. acnes secara in-vitro dan mengetahui konsentrasi minimal ekstrak yang dapat menghambat pertumbuhan bakteri P. acnes. Penelitian ini bertempat di Laboratorium Biologi FKIP Universitas Galuh, dilakukan dengan menggunakan metode eksperimen dan didesain menggunakan Rancangan Acak Lengkap (RAL). Teknik pengujian menggunakan metode sumur dengan tujuh perlakuan dan empat kali ulangan. Konsentrasi ekstrak daun belimbing wuluh yang digunakan berdasarkan hasil penelitian pendahuluan adalah 75 mgml-1, 100 mgml-1, 125 mgml-1, 150 mgml-1, 175� mgml-1, 200 mgml-1, dan 225 mgml-1. Parameter yang digunakan adalah dengan mengukur diameter zona hambat pada daerah bening sekitar sumur yaitu daerah yang tidak ditumbuhi bakteri dalam satuan milimeter. Data yang diperoleh dianalisis dengan Analisis Varian Satu Faktor (ANAVA), dan berdasarkan hasil analisis diperoleh Fhitung 285,28 lebih besar dari Ftabel (0,01)dengan taraf nyata (α) 1% sebesar 3,81 yang berarti bahwa perbedaan konsentrasi ekstrak daun belimbing wuluh berpengaruh sangat nyata terhadap diameter zona hambat pertumbuhan bakteri P. acnes yang terbentuk secara in-vitro. Berdasarkan hasil Uji Jarak Berganda Duncan diperoleh bahwa konsentrasi minimal ekstrak daun Belimbing wuluh yang dapat menghambat pertumbuhan bakteri bakteri P. acnes secara in-vitro adalah 100 mgml-1.Kata kunci: Anti bakteri, Ekstrak daun belimbing wuluh, Propionibacterium acnes, In vitr

scholarly journals Evaluation of Anti-Propionibacterium Acnes and Anti-Inflammatory Effects of Polyphenolic Extracts of Medicinal Herbs in Jordan

2019 ◽  
Vol 12 (1) ◽  
pp. 211-217 ◽  
Author(s):  
Luay Abu-Qatouseh ◽  
Eyad Mallah ◽  
Kenza Mansour
Keyword(s):  
Antimicrobial Activity ◽  
Propionibacterium Acnes ◽  
Acne Vulgaris ◽  
Interleukin 1 ◽  
Phenolic Contents ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Broth Microdilution Method ◽  
Anti Inflammatory

Acne vulgaris is one of the most common health problem where medical treatment is sought in adults worldwide. It has been long described the integral role of Propionibacterium acnes in the pathogenesis of this disease. In this study, a group of local herbs known for their antimicrobial effects were selected for the evaluation of potential anti-acnes effects in vitro. Phenolics and flavonoid contents of methanolic extracts of Eucalyptus globulus, Mentha rotundifolia, Inula viscosa, Utrica dioica, Malva sylvestris, Quercus calliprinos, Arum palaestinum and Achille aodorata collected from different regions in Jordan during 2016-2017 were screened for antimicrobial activity against Propionibacterium acnes by disc diffusion and by broth microdilution method. Measurement of release of interleukin 1 alpha from human skin explants by ELISA was used for the evaluation of anti-inflammatory effects of the herbal preparations and extracts. M. rotundifolia and E. globulus, showed the highest phenolic and flavonoid contents in contrast to M. sylvestris which showed the least phenolic contents. Moreover, polyphenolic fractions exhibited modest anti-acne activity of herbal extracts of E. globulus and A. palaestinum (MIC 0.125 mg/ml), U. dioica (0.25 mg/ml) and I. viscosa (0.5 mg/ml), compared to not significant antimicrobial activity for others (MIC >1mg/ml). Regarding anti-inflammatory effects of the tested fractions, E. globulus and A. palaestinum extracts showed inhibition of interleukin 1 alpha release by more than 60 % for concentrations of 0.5 mg/ml respectively. The presence of anti-inflammatory and anti-acne activities in the polyphenolic extracts of local medicinal plants would increase the potential of using these herbs in the control of Acne vulgaris.

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