scholarly journals Retrospective study on Cystic Echinococcosis in cattle of Italy

2017 ◽  
Vol 11 (09) ◽  
pp. 719-726 ◽  
Author(s):  
Giovanni Poglayen ◽  
Antonio Varcasia ◽  
Anna Paola Pipia ◽  
Claudia Tamponi ◽  
Maria Parigi ◽  
...  
Keyword(s):  
Cystic Echinococcosis ◽  
Southern Italy ◽  
Epidemiological Data ◽  
Economic Loss ◽  
Strain Typing ◽  
Intermediate Hosts ◽  
Direct Examination ◽  
Medical Importance ◽  
Sheep Strain

Introduction: Cystic Echinococcosis (CE) is one of the most widespread zoonosis of veterinary and medical importance still constituting a sanitary, economic and socio-cultural problem in Italy. Methodology: The aim of this study was to update epidemiological data on cattle CE in Italy. Data on CE positivity of 5,336 cattle were acquired from abattoir registers between January 2009 and July 2010. Morphobiological characterization of hydatids was performed by direct examination of liver and lungs of 1,664 animals butchered in the same slaughterhouses in 2010. Strain typing of parasites was carried out through the amplification and sequencing of nd1 and cox1 mitochondrial genes. Results: Overall CE prevalence was of 8.1% (430/5,336). Parasitological examination of hydatids showed an overall prevalence of 8.6% with a fertility rate of 0.7% (12/1,664). Regarding localization, hydatids were found in 8% of the livers and in 7.6% of the lungs, respectively. Among positive animals, higher prevalence was observed in the liver (93%) compared to lungs (88.1%) (p > 0.05). Conclusion: The economic loss due to organs condemnation related to CE in cattle amounted to almost € 24,000 per year in the examined abattoir during 2010. Sequence analysis showed the presence of G1 (sheep strain) or Echinococcus granulosus sensu strictu in all examined samples. The G1 confirmed, once more, its possible development into several intermediate hosts such as cattle, especially in areas like southern Italy and Sardinia where the lifecycle of the parasite is still to date carried on by sheep and dogs.

scholarly journals Prevalence of echinococcosis in humans, livestock and dogs in northern Italy

2011 ◽  
Vol 48 (2) ◽  
pp. 59-66 ◽  
Author(s):  
M. Manfredi ◽  
A. Cerbo ◽  
S. Zanzani ◽  
A. Moriggia ◽  
D. Fattori ◽  
...  
Keyword(s):  
Cystic Echinococcosis ◽  
Southern Italy ◽  
Epidemiological Data ◽  
National Registry ◽  
Northern Italy ◽  
Total Hospital ◽  
Lombardy Region ◽  
Total Hospital Stay ◽  
Faecal Samples ◽  
Hospital Discharge Records

AbstractThe presence of Echinococcus sp. cysts was investigated in 822 sheep, 123 goats and 112,521 cattle from Lombardy region, North Italy. Faecal samples from 40 sheepdogs were also analyzed, with 9 samples containing taeniid eggs (22.5 %), 8 samples being coproantigen-positive (20 %), and one dog from a northern province (Lecco) positively confirmed by PCR. Cystic Echinococcosis (CE) was detected in 0.36 % of sheep and in 0.29 % of cattle in 2004. No goat resulted to be infected. Data from CE patients treated in Lombardy were collected by inspecting hospital discharge records. In 2004, 156 CE-related admissions (62 % male and 38 % female) were reported in Lombardy. Total hospital stay was 1,372 days (1,286 for inpatients, 86 for outpatients). Most patients (72.4 %) were residents in Lombardy and 1.9 % were from Piedmont; the remaining patients were from central and southern Italy. According to acquired data CE resulted hypoendemic in animals in Lombardy. Prevalence rates in humans were higher than expected in this region, usually considered as non-endemic. Assessment of the prevalence of CE in humans remains a difficult, costly, time-consuming and labourintensive task. The present study suggests establishing a National Registry of Cystic Echinococcosis with the aim to highlight regional risk factors and to benefit from its matching both clinical and epidemiological data.

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

2015 ◽  
Vol 60 (4) ◽  
Author(s):  
Yosra H. Alam-Eldin ◽  
Heba E. Abdel Aaty ◽  
Mona A. Ahmed
Keyword(s):  
Ribosomal Rna ◽  
Cystic Echinococcosis ◽  
First Record ◽  
Current Status ◽  
Hydatid Cysts ◽  
Specific Pcr ◽  
Molecular Studies ◽  
Animal Isolates ◽  
Sheep Strain

AbstractFew molecular studies have identified the current status of cystic echinococcosis in Egypt. The present study aimed to ascertain the genotype(s) of Echinococcus granulosus responsible for human hydatidosis in different Egyptian governorates (regions). Animal isolates were collected from 40 camels, 5 pigs and 44 sheep. 27 human isolates were included in the present study. Specific PCR was performed and followed by DNA sequencing for mitochondrial 12S ribosomal RNA gene and BLAST analysis.The sheep cysts were not hydatid cysts. G6 genotype (camel starin) predominates in human, camel and pig isolates. G7 genotype (pig strain) was detected in two human isolates and one pig isolate. G1 genotype (sheep strain) was detected in one human isolate from Yemen and in no animal isolates. This is the first record of G7 in Egypt and G1 in Yemen.

Mareks Disease in An Aseel Chicken - Cytohistopathological Approach

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
V. Kumar ◽  
N. Pazhanivel ◽  
K. Gopal ◽  
R. Thangathurai ◽  
C. Balachandran
Keyword(s):  
Herpes Virus ◽  
Epidemiological Data ◽  
Economic Loss ◽  
Lymphoproliferative Disease ◽  
Clinical Feature ◽  
Poultry Industry ◽  
Herpès Virus

Mareks diseases (MD) is a lymphoproliferative disease of chicken affecting all organs including skin. Mareks disease is become a menace to the poultry industry owing to the serious economic loss. MD is caused by herpes virus with three serotype of MDV (Carter et al., 2006). MD is a very serious problem and it produces up to 60% of mortality in layers and 10% of mortality in broilers. Epidemiological data recorded average about 10–40% flock mortality (Arulmozi et al. 2011). The clinical feature of MD is characterized by paralysis of the wings, legs and neck with lameness, depression and death occurred. The present paper reports the occurrence of MD in Aseel chicken.

Identification and molecular characterization of a novel 55-kb deletion recurrent in southern Italy: the ItalianGγ(Aγδβ)°-thalassemia

2013 ◽  
Vol 90 (3) ◽  
pp. 214-219 ◽  
Author(s):  
Giuseppina Lacerra ◽  
Romeo Prezioso ◽  
Gennaro Musollino ◽  
Giulio Piluso ◽  
Lucia Mastrullo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Southern Italy

scholarly journals Human Cystic Echinococcosis in Lebanon: A Retrospective Study and Molecular Epidemiology

2021 ◽  
Author(s):  
Gaelle Joanny ◽  
Maria Grazia Cappai ◽  
Francesca Nonnis ◽  
Claudia Tamponi ◽  
Giorgia Dessì ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Epidemiological Data ◽  
Sensu Stricto ◽  
Control Measures ◽  
Mediterranean Countries ◽  
High Gene ◽  
Human Infections ◽  
And Control ◽  
Human Cystic Echinococcosis

Abstract Purpose Human cystic echinococcosis (CE) is a zoonotic parasitic disease that constitutes a public health challenge and a socio-economic burden in endemic areas worldwide. No specific surveillance system of CE infections in humans exists in Lebanon. The incidence and trends over time have not been documented. The current study aimed to assess the demographic and epidemiologic features of human CE surgical cases over a 14-year period in the five main regions of Lebanon. Methods From 2005 to 2018, a total of 894 surgically confirmed cases of hydatidosis were recorded from five anatomy and pathology laboratories. Results The mean annual surgical incidence was 1.23/100,000 inhabitants. Over the span of these years, the incidence increased from 0.53 to 1.94 cases/100,000 inhabitants in 2005 and 2018, respectively. CE is present in Lebanon with an uneven distribution from one region to the other with higher prevalence in Bekaa (29.0%), a rural area where sheep raising is widespread. Human CE cases were more common in females (60.1%) than in males (39.9%) and a high burden of infection was reported for the age group of 30–39 years. Besides, 66.7% of the cases expressed only liver complications whereas, 20.5% showed predilection towards lungs. The 7.8% of cases presented cysts in other organs, and 1.3% showed multiple localizations. Additionally, predominant involvement of Echinococcus granulosus sensu stricto was recorded in human infections. Comparison of Echinococcus granulosus s.s. populations from different Mediterranean countries also revealed high gene flow among this region and sharing of alleles. Conclusion The current study is a step forward to fill the gap of knowledge for the hydatidosis in Lebanon where the lack of epidemiological data and control measures have resulted in higher incidence of human CE. Graphic Abstract

scholarly journals Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv. sobria Strain K133 which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms

2021 ◽  
Vol 22 (8) ◽  
pp. 4272
Author(s):  
Katarzyna Dworaczek ◽  
Maria Kurzylewska ◽  
Magdalena Laban ◽  
Dominika Drzewiecka ◽  
Agnieszka Pękala-Safińska ◽  
...  
Keyword(s):  
Epidemiological Data ◽  
13C Nmr Spectroscopy ◽  
Freshwater Species ◽  
Fish Farms ◽  
1H And 13C Nmr ◽  
Aeromonas Veronii ◽  
Specific Polysaccharide ◽  
O Antigens ◽  
Unique Composition

In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv. sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm. The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy. It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup. Western blotting and ELISA confirmed that A. veronii bv. sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp. in Polish aquacultures. Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.

Characterization of antimicrobial resistant Salmonella enterica serovars Enteritidis and Typhimurium isolates from animal and food in Southern Italy

2010 ◽  
Vol 34 (4) ◽  
pp. 387-392 ◽  
Author(s):  
Anna Giannina Perugini ◽  
Maria Rosaria Carullo ◽  
Assunta Esposito ◽  
Vincenzo Caligiuri ◽  
Federico Capuano ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Southern Italy

Characterization of excretory–secretory products from protoscoleces of Echinococcus granulosus and evaluation of their potential for immunodiagnosis of human cystic echinococcosis

Parasitology ◽  
2004 ◽  
Vol 129 (3) ◽  
pp. 371-378 ◽  
Author(s):  
D. CARMENA ◽  
J. MARTÍNEZ ◽  
A. BENITO ◽  
J. A. GUISANTES
Keyword(s):  
Echinococcus Granulosus ◽  
Cystic Echinococcosis ◽  
Secretory Protein ◽  
Major Protein ◽  
Healthy Donors ◽  
Secretory Products ◽  
Protein Components ◽  
Human Cystic Echinococcosis

This study describes, for the first time, the characterization of excretory–secretory antigens (ES-Ag) from Echinococcus granulosus protoscoleces, evaluating their usefulness in the immunodiagnosis of human cystic echinococcosis. ES-Ag were obtained from the first 50 h maintenance of protoscoleces in vitro. This preparation contained over 20 major protein components which could be distinguished by 1-dimensional SDS–PAGE with apparent masses between 9 and 300 kDa. The culture of of protoscoleces from liver produced a greater variety of excretory–secretory protein components than those from lung. Determination of enzymatic activities of secreted proteins revealed the presence of phosphatases, lipases and glucosidases, but no proteases. These findings were compared to those obtained from somatic extracts of protoscoleces and hydatid cyst fluid products. Immunochemical characterization was performed by immunoblotting with sera from individuals infected by cystic echinococcosis (n=15), non-hydatidic parasitoses (n=19), various liver diseases (n=24), lung neoplasia (n=16), and healthy donors (n=18). Antigens with apparent masses of 89, 74, 47/50, 32, and 20 kDa showed specificity for immunodiagnosis of human hydatidosis. The 89 and 74 kDa components corresponded to antigens not yet described in E. granulosus, whereas proteins of 41–43 kDa and 91–95 kDa were recognized by the majority of the non-hydatid sera studied.

Sharing Geophysical Data for Seismic Characterization of the Matera (Southern Italy) Urban Area

2021 ◽  
Author(s):  
N. Tragni ◽  
G. Calamita ◽  
L. Lastilla ◽  
V. Belloni ◽  
R. Ravanelli ◽  
...  
Keyword(s):  
Urban Area ◽  
Southern Italy ◽  
Geophysical Data ◽  
Seismic Characterization

scholarly journals PREVALENCE OF BRUCELLOSIS IN BLACK BENGAL GOATS IN BANGLADESH

2013 ◽  
Vol 10 (1-2) ◽  
pp. 51-56 ◽  
Author(s):  
M. S. Rahman ◽  
S. Mithu ◽  
M. T. Islam ◽  
M. J. Uddin ◽  
R. R. Sarker ◽  
...  
Keyword(s):  
Epidemiological Data ◽  
Economic Loss ◽  
Cross Sectional Study ◽  
Confirmatory Test ◽  
Sectional Study ◽  
Cross Sectional ◽  
History Of ◽  
Significant Economic Loss ◽  
Sera Samples ◽  
Structured Questionnaire

Brucellosis is an important bacterial zoonotic disease causing significant economic loss in dairy industries worldwide including Bangladesh. But limited studies are devoted to determine the prevalence of brucellosis in goat in all districts of Bangladesh. Therefore, a cross-sectional study was undertaken to determine the seroprevalence of brucellosis in Black Bengal goats in Nilphamari Sadar and Kishoreganj upazillas of Nilphamari district of Bangladesh using Rose Bengal Test (RBT) as screening test and I-ELISA as confirmatory test. A total of 154 sera samples from Black Bengal goats were collected from Nilphamari district. Epidemiological data on the selected Black Bengal goats were collected using a structured questionnaire. The overall seroprevalence of brucellosis was found to be 2.59% in Black Bengal goats. A significantly (p<0.01) higher prevalence of brucellosis was found in Black Bengal goats with the history of previous abortion (33.33%). An insignificant (p>0.05) but higher prevalence of brucellosis was found in adult Black Bengal goats (>24 months) than young. The prevalence was relatively higher in cross-bred than pure Black Bengal goats, in female than male and in pregnant than non-pregnant Black Bengal goats. The result of the study will provide baseline data for control of brucellosis in goat in Bangladesh.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15646

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