scholarly journals The use of lactic acid bacteria isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), as growth promoters in fish fed low protein diets

2017 ◽  
Vol 41 (3) ◽  
pp. 490-497
Author(s):  
Maurilio Lara-Flores ◽  
Miguel A. Olvera-Novoa

In this study, the effect as growth promoter of five lactic acid strains (Enterococcus faecium, E. durans, Leuconostoc sp., Streptococcus sp. I and Streptococcus sp. II), isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), was evaluated. Eight isocaloric diets were formulated: one containing 40% of protein as positive control, and seven with 27% protein. Five diets with 27% protein were supplemented with one of the isolated lactic acid bacteria in a concentration of 2.5x106 cfu g-1 of diet. A commercial probiotic based on S. faecium and Lactobacillus acidophilus was added at the same concentration to one 27% protein diet as a comparative diet, and the last diet was not supplemented with bacteria (negative control). Tilapia fry (280 mg basal weight) stocked in 15 L aquaria at a density of two per liter were fed for 12 weeks with experimental diets. Results showed that fry fed with native bacteria supplemented diets presented significantly higher growth and feeding performance than those fed with control diet. Treatment with Streptococcus sp. I isolated from the intestine of Tilapia produced the best growth and feeding efficiency, suggesting that this bacteria is an appropriate native growth promoter.

2012 ◽  
Vol 4 (1) ◽  
Author(s):  
Ana A Zapata ◽  
Maurilio Lara-Flores

The intestinal microflora of Nile tilapia (Oreochromis niloticus) fish was studied to isolate and identify lactic acid bacteria as new probiotic. A total of five lactic acid bacteria were isolated from intestine to evaluate with antibacterial properties. These strains were identified by conventional and molecular techniques as: Enterococcus faecium, Leuconostocmesenteroides, Lactobacillus fermentum, Lactobacillus plantarum, Enterococcus durans. Leuc.mesenteroides present more ability to inhibit growth of fish pathogens bacteria and selected as possible probiotic bacteria to use in aquaculture.


Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3199
Author(s):  
Alejandra Domínguez-Negrete ◽  
Sergio Gómez-Rosales ◽  
María de Lourdes Angeles ◽  
Luis Humberto López-Hernández ◽  
Tercia Cesaria Reis de Souza ◽  
...  

Different sources and inclusion levels of humic substances (HS) have been tested in broiler rations as an alternative to the addition of growth promoter antibiotics (GPA) with promising results. The current study was carried out to assess the influence of HS extracted from worm compost on broiler production parameters, carcass yield, tibia characteristics, lactic acid bacteria (LAB) counts, excretion of Eimeria oocysts, and antioxidant status of breast meat. A total of 1200 broilers were used, housed in groups of 30 per pen, and assigned to five treatments: 1 = basal diet with GPA (positive control), 2 = basal diet without GPA (negative control), 3–5 = basal diet with 0.15, 0.30, and 0.45% HS, respectively. The data was subjected to a variance analysis and orthogonal contrasts. The FI decreased linearly (p < 0.05) from 1–14, 29–42, and 1–42 days as the inclusion of HS in the feed increased. The FCR had quadratic responses (p < 0.01) from 29–42 and 1–42 days concerning the HS inclusion levels. Lactic acid bacteria was higher (p ˂ 0.05) in ten-day-old chicks with 0.45% HS in the diet. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity antioxidant potential decreased linearly (p < 0.05) concerning increasing HS in the feed. The results indicate that HS can be used as growth promoters in broiler feeds.


2018 ◽  
Vol 10 (3) ◽  
pp. 193-202
Author(s):  
NEDAL MOHAMMED SIDDIG SWAR ◽  
ASAAD HASSAN WIDAA MOHAMED

Swar NMS, Mohamed AHW. 2018. Incorporation of dietary palm date seeds in all-male Nile tilapia (Oreochromis niloticus) diets. Nusantara Bioscience 10: 193-202. The study utilizes a 45-days randomized factorial design 3×2, three levels (25, 50 and 75%) of palm date seeds, three levels (non, with and without) of 3% bakery yeast (Saccharomyces cerevisiae) and three replicates. The observation was carried out in twenty-one plastic aquaria. All-male Nile tilapia (Oreochromis niloticus) fingerlings were put in each aquarium. Each aquarium was provided with well-aerated and triggered dechlorinated tap water with an average weight of 1.9±1.11 g/fish (10 fish/aquaria) and a total length of 5.18±0.69 cm/fish. Fish were given food three times/day (10 days, at 8.30,11.30, 3.30 A.M) at a rate of 12, 8 and 4% of body weight, to examine the impact of partial substitution of animal protein (fish meal) in the diet on growth achievement, carcass contexture, feed utilization, condition factor (k) and feed expense. Seven experimental diets were prepared; control diet (T0, CP 36.84) concluding 45% (fish meal) as animal protein and 0% (palm date seed meal) plant protein. Tested diets (T1, T2 and T3) concluding 25, 50, 75% with 3% bakery yeast (CP 35.71, 35.53, 34.74) and 25, 50, 75% without yeast (CP36.01, 36.27 and 35.57) respectively ; so, they substitute about 75, 50, and 25 of fish meal diet respectively. The outcomes showed that, tilapia were given food on T1, T2 and T4 diets (25, 50, and 25%) substitution with and without yeast respectively registered the greater growth achievement, feed and protein utilization than other experimental diets such as control diet, also they indicate the highest condition factor (K) grades which state that the fish are in decent health. Tilapia were given food by diet T3 (25% fish meal) T5, T6 (50 and 25% fish meal) with and without yeast respectively possessed deficient growth and diverged remarkably (p>0.05) from the other diets. Diet contexture remarkably had an impact on carcass contexture. These data prompted that Palm date seed with and without yeast S. cerevisiae can, to a certain extent, substitute fish meal (animal protein) in a diet for all-male Nile tilapia fingerlings at level-up to 50 and 25% with and without yeast respectively, without any unfavorable result on accretion achievement. Furthermore, fish diet, partially substituted with 3% yeast, resulted from superior accretion achievement than other diets within the present experimental circumstance. This study showed that there is an economic efficiency of confounding palm date seed (plant protein) as partial substitution of fish meal (animal protein) with and without yeast in all-male Nile tilapia, that it could decrease the expense of feeds.


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