Docosahexaenoic acid supplementation alleviates behavioral memory impairment caused via repeated administration of sevoflurane in aged rats

Abstract The current study aimed to explore the effect of docosahexaenoic acid (DHA) on the behavioral memory impairment caused by repeated anesthesia of sevoflurane in aged rats. A total of 54 Sprague‑Dawley (SD) aged rats were randomly divided into six groups: Blank control group (Control), sevoflurane group (Sev), DHA group (3g/kg), Sev + DHA (0.3g/kg) group, Sev + DHA (1g/kg) group and Sev + DHA (3g/kg) group. Morris water maze experiment was used to evaluate the learning and memory ability. Hematoxylin and eosin (H&E) staining was used to observe histological changes. The content of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected. Immunohistochemistry and western blot analysis were used to determine the expression of proteins. Rats were indicated to exhibit prolonged escape latency following sevoflurane anesthesia. The number of times taken to cross the platform and the time for target quadrant stay were also demonstrated to be significantly reduced. Rats treated with different doses of DHA were revealed to exhibit reduced escape latency. The number of times taken to cross the platform and the time for target quadrant stay increased. Histopathological examination indicated that DHA treatment ameliorated the pathological change of the rats brain tissue. Furthermore, the expression of Nrf2 and HO-1 protein were demonstrated to be significantly increased. The present study revealed that DHA has a protective effect on learning and memory impairment in aged rats induced by repeated sevoflurane anesthesia, and the mechanism may be associated with the Nrf2/HO-1 signaling pathway.

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Interventional Effects of DHA on Behavioral Memory Impairment Caused by Repeated Anesthesia of Sevoflurane in Aged Rats

10.21203/rs.3.rs-29872/v1 ◽

2020 ◽

Author(s):

Ming Tian ◽

Kezhong Li ◽

Xiaoling Zhao

Keyword(s):

Signaling Pathway ◽

Learning And Memory ◽

Memory Impairment ◽

Escape Latency ◽

Control Group ◽

High Dose ◽

Group Model ◽

Aged Rats ◽

Sevoflurane Anesthesia ◽

Behavioral Memory

Abstract Backgroud: The current study aimed to explore the effects of Docosahexaenoic acid (DHA) on the behavioral memory impairment induced by repeated anesthesia of sevoflurane in aged rats. Methods: A total of 45 Sprague‑Dawley (SD) aged rats were randomly divided into five groups: Blank control group (Control), sevoflurane group (Model), low-dose DHA group (L-DHA; 0.3g/kg), medium-dose DHA group (M-DHA; 1g/kg) and high-dose DHA group (H-DNA; 3g/kg). Morris water maze experiment was used to evaluate the learning and memory ability of rats. Hematoxylin and eosin staining was used to observe histological changes in the hippocampus. Immunohistochemistry and western blot analysis were used to determine the expression of the Nuclear factor erythroid-2 (NF-E2)-related factor 2(Nrf2)/hemeoxygenase-1 (HO-1) signaling pathway. Results: Rats were indicated to exhibit prolonged escape latency following sevoflurane anesthesia. The number of times taken to cross the platform and the time for target quadrant stay were also demonstrated to be significantly reduced. Rats treated with different doses of DHA were revealed to exhibit reduced escape latency. The number of times taken to cross the platform and the time for target quadrant stay increased. Histopathological examination indicated that DHA attenuated the brain function of the rats that were repeatedly anesthetized using sevoflurane. Furthermore, the expression of Nrf2 and HO-1 protein were demonstrated to be significantly increased. Conclusions: The present study revealed that DHA has a protective effect on learning and memory impairment in aged rats induced by repeated sevoflurane anesthesia, and the mechanism may be associated with the Nrf2/HO-1 signaling pathway.

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Docosahexaenoic acid supplementation-increased oxidative damage in bone marrow DNA in aged rats and its relation to antioxidant vitamins

Free Radical Research ◽

10.1080/10715760100300361 ◽

2001 ◽

Vol 34 (4) ◽

pp. 427-435 ◽

Cited By ~ 21

Author(s):

Keizo Umegaki ◽

Michio Hashimoto ◽

Hiroshi Yamasaki ◽

Yoshimid Fujii ◽

Mika Yoshimura ◽

...

Keyword(s):

Bone Marrow ◽

Docosahexaenoic Acid ◽

Oxidative Damage ◽

Antioxidant Vitamins ◽

Aged Rats ◽

Acid Supplementation

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DHA supplementation improves behavioral memory impairment and alleviates oxidative stress in hippocampal caused by repeated anesthesia of sevoflurane in aged rats

10.21203/rs.3.rs-29872/v2 ◽

2020 ◽

Author(s):

Ming Tian ◽

Kezhong Li ◽

Xiaoling Zhao

Keyword(s):

Signaling Pathway ◽

Learning And Memory ◽

Memory Impairment ◽

Escape Latency ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Group Model ◽

Aged Rats ◽

Sevoflurane Anesthesia ◽

Behavioral Memory

Abstract Backgroud: The current study aimed to explore the effects of docosahexaenoic acid (DHA) on the behavioral memory impairment induced by repeated anesthesia of sevoflurane in aged rats. Methods: A total of 54 Sprague‑Dawley (SD) aged rats were randomly divided into five groups: Blank control group (Control), sevoflurane group (Model), DHA group (3g/kg), Sev + DHA (0.3g/kg) group, Sev + DHA (1g/kg) group and Sev + DHA (3g/kg) group. Morris water maze experiment was used to evaluate the learning and memory ability. Hematoxylin and eosin (H&E) staining was used to observe histological changes in the hippocampus. Enzyme linked immunosorbent assay (ELISA) was used to detect the content of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in hippocampus. Immunohistochemistry and western blot analysis were used to determine the expression of the Nuclear factor erythroid-2 (NF-E2)-related factor 2(Nrf2)/ hemeoxygenase-1 (HO-1) signaling pathway. Results: Rats were indicated to exhibit prolonged escape latency following sevoflurane anesthesia. The number of times taken to cross the platform and the time for target quadrant stay were also demonstrated to be significantly reduced. Rats treated with different doses of DHA were revealed to exhibit reduced escape latency. The number of times taken to cross the platform and the time for target quadrant stay increased. Histopathological examination indicated that DHA ameliorated the brain function of the rats that were repeatedly anesthetized using sevoflurane. Furthermore, the expression of Nrf2 and HO-1 protein were demonstrated to be significantly increased. Conclusions: The present study revealed that DHA has a protective effect on learning and memory impairment in aged rats induced by repeated sevoflurane anesthesia, and the mechanism may be associated with the Nrf2/HO-1 signaling pathway.

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