Genome-wide microarray gene expression, array-CGH analysis, and telomerase activity in advanced ovarian endometriosis: A high degree of differentiation rather than malignant potential

The essential nature of family therapy, the role of the therapist in the therapeutic process, and the need for family therapists to possess a high degree of differentiation of self are examined. As a prerequisite to conducting successful family systems therapy, therapists must examine their own family of origin.

Download Full-text

Differences in gene expression array of superantigen-stimulated PBMC from patients with atopic dermatitis (AD) vs normal subjects

Journal of Allergy and Clinical Immunology ◽

10.1016/s0091-6749(02)81360-4 ◽

2002 ◽

Vol 109 (1) ◽

pp. S86-S86

Author(s):

Ichiro Nomura ◽

Peck Y Ong ◽

Bifeng Gao ◽

Mark Boguniewicz ◽

Donald Ym Leung

Keyword(s):

Gene Expression ◽

Atopic Dermatitis ◽

Normal Subjects ◽

Gene Expression Array ◽

Expression Array

Download Full-text

Tools of Interregional Cooperation in the Public Administration System

REGIONOLOGY ◽

10.15507/2413-1407.115.029.202102.250-282 ◽

2021 ◽

Vol 29 (2) ◽

pp. 250-282

Author(s):

Vladimir V. Klimanov ◽

Sofia M. Kazakova ◽

Vita A. Yagovkina

Keyword(s):

Russian Federation ◽

Regional Cooperation ◽

Public Procurement ◽

Financial Assessment ◽

Scientific Potential ◽

Interregional Cooperation ◽

The Russian Federation ◽

Degree Of Differentiation ◽

Regional Authorities ◽

High Degree

Introduction. The development of modern Russia is characterized by a high degree of differentiation in the level of socio-economic development of the constituent entities of the Russian Federation. Interregional cooperation is one of the tools to overcome this differentiation. Although new forms of such cooperation have been emerging, its potential remains underestimated. The purpose of the study is to analyze the existing and prospective forms of cooperation between the regions of Russia. Materials and Methods. The study based on a structural and substantive analysis of regulatory legal acts of the federal and regional levels, as well as on strategic planning documents, various forms of budget reporting, bilateral and multilateral agreements between the constituent entities of the Russian Federation aimed at identifying various areas, tools and mechanisms for ensuring interregional cooperation. Results. The authors have considered the tools of interregional cooperation that involve direct financial participation of regional authorities or economic entities. The role and prospects of horizontal subsidies and budget loans provided by one region to another have been identified, their legal and regulatory justification has been given; a financial assessment has been made, and the identified practices have been analyzed. In terms of the implementation of public procurement from suppliers based in other regions, the authors have estimated the degree of how closed or open the constituent entities of the Russian Federation are. Discussion and Conclusion. The authors have confirmed the idea that interregional cooperation in Russia is still underdeveloped and lacks legislative and scientific-methodological justification. The authors also assess the prospects for further development of interregional cooperation in Russia and suggest a number of measures to promote it. The results of the study can be used in the interests of the authorities to improve the system of regulation of various mechanisms of regional cooperation, as well as to develop the scientific potential in this area.

Download Full-text

Solar simulated ultraviolet radiation inactivates HCoV-NL63 and SARS-CoV-2 coronaviruses at environmentally relevant doses

10.1101/2021.06.25.449831 ◽

2021 ◽

Author(s):

Georg T. Wondrak ◽

Jana Jandova ◽

Spencer J. Williams ◽

Dominik Schenten

Keyword(s):

Gene Expression ◽

Stress Response ◽

Uv Light ◽

Gene Expression Array ◽

Array Analysis ◽

Expression Array ◽

Response Gene ◽

Full Spectrum ◽

Solar Uv ◽

Stress Response Gene

The germicidal properties of short wavelength ultraviolet C (UVC) light are well established and used to inactivate many viruses and other microbes. However, much less is known about germicidal effects of terrestrial solar UV light, confined exclusively to wavelengths in the UVA and UVB regions. Here, we have explored the sensitivity of the human coronaviruses HCoV-NL63 and SARS-CoV-2 to solar-simulated full spectrum ultraviolet light (sUV) delivered at environmentally relevant doses. First, HCoV-NL63 coronavirus inactivation by sUV-exposure was confirmed employing (i) viral plaque assays, (ii) RT-qPCR detection of viral genome replication, and (iii) infection-induced stress response gene expression array analysis. Next, a detailed dose-response relationship of SARS-CoV-2 coronavirus inactivation by sUV was elucidated, suggesting a half maximal suppression of viral infectivity at low sUV doses. Likewise, extended sUV exposure of SARS-CoV-2 blocked cellular infection as revealed by plaque assay and stress response gene expression array analysis. Moreover, comparative (HCoV-NL63 versus SARS-CoV-2) single gene expression analysis by RT-qPCR confirmed that sUV exposure blocks coronavirus-induced redox, inflammatory, and proteotoxic stress responses. Based on our findings, we estimate that solar ground level full spectrum UV light impairs coronavirus infectivity at environmentally relevant doses. Given the urgency and global scale of the unfolding SARS-CoV-2 pandemic, these prototype data suggest feasibility of solar UV-induced viral inactivation, an observation deserving further molecular exploration in more relevant exposure models.

Download Full-text