scholarly journals Sulforaphane suppresses polyinosinic‑polycytidylic acid‑stimulated release of cytokines, chemokines and MMPs by human corneal fibroblasts

2020 ◽  
Vol 22 (6) ◽  
pp. 5463-5471
Author(s):  
Pingping Liu ◽  
Ye Liu ◽  
Hui Zheng ◽  
Xiaoshuo Zheng ◽  
Xiuxia Yang ◽  
...  
Keyword(s):  
Polycytidylic Acid ◽  
Corneal Fibroblasts ◽  
Polyinosinic Polycytidylic Acid

scholarly journals Polyinosinic-Polycytidylic Acid Is the Most Effective TLR Adjuvant for SIV Gag Protein–Induced T Cell Responses In Nonhuman Primates

2013 ◽  
Vol 190 (8) ◽  
pp. 4103-4115 ◽  
Author(s):  
Haesun Park ◽  
Lauren Adamson ◽  
Tae Ha ◽  
Karl Mullen ◽  
Shoko I. Hagen ◽  
...  
Keyword(s):  
T Cell ◽  
Nonhuman Primates ◽  
T Cell Responses ◽  
Gag Protein ◽  
Cell Responses ◽  
Polycytidylic Acid ◽  
Polyinosinic Polycytidylic Acid

scholarly journals Polyinosinic: Polycytidylic Acid and Murine Cytomegalovirus Modulate Expression of Murine IL-10 and IL-21 in White Adipose Tissue

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 569
Author(s):  
Pablo Garcia-Valtanen ◽  
Ruth Marian Guzman-Genuino ◽  
John D. Hayball ◽  
Kerrilyn R. Diener
Keyword(s):  
Adipose Tissue ◽  
B Cells ◽  
White Adipose Tissue ◽  
Ex Vivo ◽  
Interleukin 10 ◽  
Poly I:C ◽  
Murine Cytomegalovirus ◽  
Polycytidylic Acid ◽  
Polyinosinic Polycytidylic Acid

White adipose tissue (WAT) produces interleukin-10 and other immune suppressors in response to pathogen-associated molecular patterns (PAMPs). It also homes a subset of B-cells specialized in the production of IL-10, referred to as regulatory B-cells. We investigated whether viral stimuli, polyinosinic: polycytidylic acid (poly(I:C)) or whole replicative murine cytomegalovirus (MCMV), could stimulate the expression of IL-10 in murine WAT using in vivo and ex vivo approaches. Our results showed that in vivo responses to systemic administration of poly(I:C) resulted in high levels of endogenously-produced IL-10 and IL-21 in WAT. In ex vivo WAT explants, a subset of B-cells increased their endogenous IL-10 expression in response to poly(I:C). Finally, MCMV replication in WAT explants resulted in decreased IL-10 levels, opposite to the effect seen with poly(I:C). Moreover, downregulation of IL-10 correlated with relatively lower number of Bregs. To our knowledge, this is the first report of IL-10 expression by WAT and WAT-associated B-cells in response to viral stimuli.

Involvement of protein kinase C in macrophage activation by poly(I.C)

1994 ◽  
Vol 266 (1) ◽  
pp. C134-C142 ◽  
Author(s):  
F. R. Lake ◽  
E. C. Dempsey ◽  
J. D. Spahn ◽  
D. W. Riches
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Phorbol Myristate Acetate ◽  
Mrna Levels ◽  
Myristate Acetate ◽  
Western Blots ◽  
Level Of Involvement ◽  
Polycytidylic Acid ◽  
Kinase C ◽  
Polyinosinic Polycytidylic Acid

The expression of cytocidal activity is initiated by the interaction of macrophages with priming [e.g., interferon (IFN)] and triggering stimuli (polyinosinic-polycytidylic acid). We have shown that the triggering step can be initiated in a Ca(2+)-dependent fashion and hypothesized that protein kinase C (PKC) may couple the Ca2+ signal to the expression of a gene product, Bf, that accompanies the expression of macrophage cytocidal activity. Exposure of IFN-primed macrophages to polyinosinic-polycytidylic acid in the presence of the PKC inhibitors H-7 or sphingosine or after downregulation of PKC with phorbol myristate acetate markedly inhibited Bf synthesis. Western blots of macrophage lysates revealed the presence of the alpha-, delta-, and zeta-isozymes of PKC, and all were found to be downregulated by phorbol myristate acetate. Inhibition of PKC also prevented the increase in IFN-beta mRNA levels and partially blocked the response to IFN-beta. These data suggest that the alpha-, delta-, and zeta-isozymes of PKC are involved in signaling leading to Bf expression and that the level of involvement is restricted to the induction and response to IFN-beta.

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