The detection of pollen in royal jelly of honey bees (Apis mellifera)

2011 ◽  
Vol 3 (3) ◽  
pp. 137-139 ◽  
Author(s):  
Leila Nunes Morgado ◽  
Ortrud Monika Barth
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Royal Jelly

scholarly journals Rearing Drones in Queen Cells of Apis mellifera Honey Bees

2016 ◽  
Vol 60 (2) ◽  
pp. 119-128
Author(s):  
Georgios Goras ◽  
Chrysoula Tananaki ◽  
Sofia Gounari ◽  
Elissavet Lazaridou ◽  
Dimitrios Kanelis ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Larval Development ◽  
Honey Bees ◽  
Royal Jelly ◽  
Early Stage ◽  
Egg Laying ◽  
Horizontal Position ◽  
Before And After ◽  
Queen Larvae ◽  
As If

Abstract We investigated the rearing of drone larvae grafted in queen cells. From the 1200 drone larvae that were grafted during spring and autumn, 875 were accepted (72.9%) and reared as queens. Drone larvae in false queen cells received royal jelly of the same composition and of the same amounts as queen larvae. Workers capped the queen cells as if they were drones, 9-10 days after the egg laying. Out of 60 accepted false queen cells, 21 (35%) were capped. The shape of false queen cells with drone larvae is unusually long with a characteristically elongate tip which is probably due to the falling of larvae. Bees start the destruction of the cells when the larvae were 3 days old and maximised it before and after capping. Protecting false queen cells in the colony by wrapping, reversing them upside down, or placing in a horizontal position, did not help. The only adult drones that emerged from the false queen cells were those protected in an incubator and in push-in cages. Adult drones from false queen cells had smaller wings, legs, and proboscis than regular drones. The results of this study verify previous reports that the bees do not recognise the different sex of the larvae at least at the early stage of larval development. The late destruction of false queen cells, the similarity in quality and quantity of the produced royal jelly, and the bigger drone cells, allow for the use of drone larvae in cups for the production of royal jelly.

scholarly journals Transcriptional Control of Honey Bee (Apis mellifera) Major Royal Jelly Proteins by 20-Hydroxyecdysone

Insects ◽  
2018 ◽  
Vol 9 (3) ◽  
pp. 122 ◽  
Author(s):  
Paul Winkler ◽  
Frank Sieg ◽  
Anja Buttstedt
Keyword(s):  
Gene Expression ◽  
Apis Mellifera ◽  
Juvenile Hormone ◽  
Honey Bee ◽  
Honey Bees ◽  
Transcriptional Control ◽  
Royal Jelly ◽  
Adult Worker ◽  
Gene Expression Dynamics

One of the first tasks of worker honey bees (Apis mellifera) during their lifetime is to feed the larval offspring. In brief, young workers (nurse bees) secrete a special food jelly that contains a large amount of unique major royal jelly proteins (MRJPs). The regulation of mrjp gene expression is not well understood, but the large upregulation in well-fed nurse bees suggests a tight repression until, or a massive induction upon, hatching of the adult worker bees. The lipoprotein vitellogenin, the synthesis of which is regulated by the two systemic hormones 20-hydroxyecdysone and juvenile hormone, is thought to be a precursor for the production of MRJPs. Thus, the regulation of mrjp expression by the said systemic hormones is likely. This study focusses on the role of 20-hydroxyecdysone by elucidating its effect on mrjp gene expression dynamics. Specifically, we tested whether 20-hydroxyecdysone displayed differential effects on various mrjps. We found that the expression of the mrjps (mrjp1–3) that were finally secreted in large amounts into the food jelly, in particular, were down regulated by 20-hydroxyecdysone treatment, with mrjp3 showing the highest repression value.

scholarly journals Longevity extension of worker honey bees (Apis mellifera) by royal jelly: optimal dose and active ingredient

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3118 ◽  
Author(s):  
Wenchao Yang ◽  
Yuanyuan Tian ◽  
Mingfeng Han ◽  
Xiaoqing Miao
Keyword(s):  
Apis Mellifera ◽  
Ammonium Sulfate ◽  
Honey Bees ◽  
Royal Jelly ◽  
Sucrose Solution ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Water Soluble ◽  
Main Component ◽  
Colony Productivity

In the Western honey bee, Apis mellifera, queens and workers have different longevity although they share the same genome. Queens consume royal jelly (RJ) as the main food throughout their life, including as adults, but workers only eat worker jelly when they are larvae less than 3 days old. In order to explore the effect of RJ and the components affecting longevity of worker honey bees, we first determined the optimal dose for prolonging longevity of workers as 4% RJ in 50% sucrose solution, and developed a method of obtaining long lived workers. We then compared the effects of longevity extension by RJ 4% with bee-collected pollen from rapeseed (Brassica napus). Lastly, we determined that a water soluble RJ protein obtained by precipitation with 60% ammonium sulfate (RJP60) contained the main component for longevity extension after comparing the effects of RJ crude protein extract (RJCP), RJP30 (obtained by precipitation with 30% ammonium sulfate), and RJ ethanol extract (RJEE). Understanding what regulates worker longevity has potential to help increase colony productivity and improve crop pollination efficiency.

scholarly journals Evaluation of Apis mellifera Carniolan and Africanized honey bees in royal jelly production

2004 ◽  
Vol 47 (3) ◽  
pp. 469-476 ◽  
Author(s):  
Gisele Fernanda Mouro ◽  
Vagner de Alencar Arnaut de Toledo
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Royal Jelly ◽  
Africanized Honey Bees ◽  
Africanized Honeybees ◽  
Lower Production

Investigations were carried out to evaluate Africanized and Carniolan hybrid Apis mellifera honeybees in royal jelly production. Ten colonies were randomly collected in nature and housed in Langstroth beehives. Each hive was having two nuclei with five frames. In superior nucleus frame was containing 28 acrylic cups in two bars. The five colonies with the best production remained in the experiment and their queens replaced by the respective daughters. Carniolan queens were placed in the five Africanized honeybees colonies with lower production. The hybrid Carniolan showed a higher production (P=0.0001) of royal jelly per colony, in three-day collect (3.95 ± 2.92 g) when compared to the Africanized honeybees (2.23 ± 2.13 g). The Carniolan also had a higher percentage of larvae acceptance (55.45 ± 36.7%) compared to the Africanized honeybees (35.8 ± 28.3%).

scholarly journals Antimicrobial potential of royal jelly from supplemented Apis mellifera africanized honey bees

2010 ◽  
Vol 1 (1) ◽  
pp. 17
Author(s):  
Mariellen Guilherme dos Santos ◽  
Dayane Aparecida dos Santos ◽  
Angela Kwiakowski ◽  
Maria Josiane Sereia ◽  
Lívia Bracht
Keyword(s):  
Antimicrobial Activity ◽  
Apis Mellifera ◽  
Palm Oil ◽  
Honey Bees ◽  
Royal Jelly ◽  
Linseed Oil ◽  
Diffusion Method ◽  
Dependent Manner ◽  
Disk Diffusion Method ◽  
Africanized Honey Bees

<p>The aim of this paper was to study the in vitro antimicrobial activity of royal jelly obtained from supplemented honey bees against the microorganism Staphylococcus aureus using the disk diffusion method. The royal jelly was obtained from Apis mellifera africanized honey bees fed with complete supplement constituted of palm oil plus linseed oil, palm oil alone, linseed oil alone and with no supplementation. Samples obtained from the different supplementations were applied to paper disks in the concentration range 0 -100%.&nbsp; All the samples of royal jelly were able to inhibit the growth of S. aureus in a dose-dependent manner. There was no difference between the antimicrobial activity of the different samples of royal jelly obtained from supplemented or not supplemented bees.</p><p>&nbsp;</p><p><span>DOI:&nbsp;<a href="http://dx.doi.org/10.14685/rebrapa.v4i1.102">http://dx.doi.org/10.14685/rebrapa.v1i1.3</a></span></p><p>&nbsp;</p>

INFLUENCE OF AGE AND POPULATION SIZE ON OVARIAN DEVELOPMENT, AND OF TROPHALLAXIS ON OVARIAN DEVELOPMENT AND VITELLOGENIN TITRES OF QUEENLESS WORKER HONEY BEE (HYMENOPTERA: APIDAE)

1999 ◽  
Vol 131 (5) ◽  
pp. 695-706 ◽  
Author(s):  
Huarong Lin ◽  
Mark L. Winston ◽  
Norbert H. Haunerland ◽  
Keith N. Slessor
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Royal Jelly ◽  
Ovarian Development ◽  
Egg Laying ◽  
Direct Access ◽  
Ovary Development ◽  
Dominance Status ◽  
Access To Food

AbstractWe examined the factors that might influence ovary development in worker honey bees, Apis mellifera L. Queenless workers at different ages (≤ 12 h, and 4, 8, and 21 d) were tested in cages for ovarian development. Newly emerged, 4- and 8-d-old, and 21-d-old workers had medium-, large-, and small-sized ovaries, respectively, suggesting that of the worker ages tested only 4- and 8-d-old workers are likely to become egg layers in a queenless colony. Also, we compared ovarian development of newly emerged workers that were caged for 14 d and allowed to consume either pollen or royal jelly to that of another group of workers similarly caged but screened so that they could only obtain food via trophallaxis from young bees. Ovaries of newly emerged workers that received food from young bees were as well developed as those of newly emerged workers allowed to take pollen or royal jelly directly. Screened workers also had lower but still elevated vitellogenin levels compared with bees having direct access to food. These results indicate that nurse-age bees functioning as pollen-digesting units affect the ovarian development of other workers and to a lesser extent vitellogenesis via food exchange. We compared the influence of group sizes of 25, 125, and 600 bees per cage on ovarian development for 14 d. The two groups of 25 and 125 bees had similar mean ovary scores, and higher scores than a group of 600 bees. Our findings suggest that nurse-age bees could play an important role in mediating worker fertility via trophallaxis, possibly by differentiating worker dominance status, and generally only young workers become fertile when a queen is lost in a colony. Vitellogenin is a more sensitive parameter to measure bee fertility, and might be a useful tool to further explore ovary development and egg laying in worker social insects. We recommend measuring haemolymph vitellogenin titres and (or) oocyte length of workers in a group of 25 bees per cage, supplied with 50% royal jelly in honey as a standard method to assess honey bee worker fertility in future experiments.

scholarly journals Comparison of the nutrient composition of royal jelly and worker jelly of honey bees (Apis mellifera)

Apidologie ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Ying Wang ◽  
Lanting Ma ◽  
Weixing Zhang ◽  
Xuepei Cui ◽  
Hongfang Wang ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bees ◽  
Royal Jelly ◽  
Nutrient Composition

scholarly journals The Transition Ratio of Nosema spp. Spores From Colonies to Honey Versus Honey to Colonies

2018 ◽  
Vol 11 (1) ◽  
pp. 72
Author(s):  
Ceren Sarıbıyık ◽  
Aslı Özkırım
Keyword(s):  
Risk Assessment ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Digestive System ◽  
Royal Jelly ◽  
Nosema Apis ◽  
Major Threat ◽  
Bee Disease ◽  
Bee Colonies

Honey bees (Apis mellifera L.) are insects that have an important role in plant pollination as well as production of benefical products such as honey, propolis, pollen, royal jelly, bee venom and beeswax. There is a growing interest in bee diseases and loss, which is a major threat to the economy and human health. Nosemosis is an adult honey bee disease which effects its digestive system mostly. The cause for the disease is Nosema apis or Nosema ceranae but the two can be seen together, too. This article aims to explore the transmission of Nosemosis and its effect on honey. For this purpose, a field study was conducted in Muğla province, where 51 pieces were collected from bee yards as spring samples and 51 pieces from bee yards as autumn samples during these two seasons, and 51 honey samples from bee yards were examined during the honey harvest. The results revealed that Nosema spp. which was obtained from honey bee samples collected in spring was more effective on honey samples. Nosema spp. was found to have a linear relation with the infection in the hive. It was determined that the percentage of Nosema spores seen in adults was 1.63%. This result contributed to the literature by providing this ratio used in estimating the level of infection in the colonies by means of honey sampling. Furthermore this is the first study where the contamination risk of honey from the infected colonies is calculated. Hopefully, this study can provide background for further research on the protection of bee colonies and risk assessment against Nosema spp. disease.

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