scholarly journals A DNA barcode-assisted annotated checklist of the spider (Arachnida, Araneae) communities associated to white oak woodlands in Spanish National Parks

2018 ◽  
Vol 6 ◽  
Author(s):  
Luís Crespo ◽  
Marc Domènech ◽  
Alba Enguídanos ◽  
Jagoba Malumbres-Olarte ◽  
Pedro Cardoso ◽  
...  

A large scale semi-quantitative biodiversity assessment was conducted in white oak woodlands in areas included in the Spanish Network of National Parks, as part of a project aimed at revealing biogeographic patterns and identify biodiversity drivers. The semi-quantitative COBRA sampling protocol was conducted in sixteen 1-ha plots across six national parks using a nested design. All adult specimens were identified to species level based on morphology. Uncertain delimitations and identifications due to either limited information of diagnostic characters or conflicting taxonomy were further investigated using DNA barcode information.We identified 376 species belonging to 190 genera in 39 families, from the 8,521 adults found amongst the 20,539 collected specimens. Faunistic results include the discovery of 7 new species to the Iberian Peninsula, 3 new species to Spain and 11 putative new species to science. As largely expected by environmental features, the southern parks showed a higher proportion of Iberian and Mediterranean species than the northern parks, where the Palearctic elements were largely dominant. The analysis of approximately 3,200 DNA barcodes generated in the present study, corroborated and provided finer resolution to the morphologically based delimitation and identification of specimens in some taxonomically challenging families. Specifically, molecular data confirmed putative new species with diagnosable morphology, identified overlooked lineages that may constitute new species, confirmed assignment of specimens of unknown sexes to species and identified cases of misidentifications and phenotypic polymorphisms.

ZooKeys ◽  
2021 ◽  
Vol 1054 ◽  
pp. 85-93
Author(s):  
Ruiwen Wu ◽  
Xiongjun Liu ◽  
Takaki Kondo ◽  
Shan Ouyang ◽  
Xiaoping Wu

We diagnose and describe a new freshwater mussel species of the genus Inversidens, I. rentianensissp. nov. from Jiangxi Province, China based on morphological characters and molecular data. This paper includes a morphological description and photograph of the holotype, and partial sequences of mitochondrial COI as DNA barcode data.


ZooKeys ◽  
2021 ◽  
Vol 1037 ◽  
pp. 57-71
Author(s):  
Zhaoyang Chen ◽  
Dengqing Li ◽  
Daiqin Li ◽  
Xin Xu

We diagnose and describe three new species of the primitively segmented spider genus Songthela from Guizhou Province, China, based on morphological characters and molecular data: S. liuisp. nov. (♂♀), S. tianzhusp. nov. (♂♀), and S. yupingsp. nov. (♂♀). We provide the genetic distances within and among the three new species based on the DNA barcode gene, cytochrome c oxidase subunit I (COI) to support our descriptions. We also provide the COI GenBank accession codes for the three new species for future identification.


Zootaxa ◽  
2020 ◽  
Vol 4747 (1) ◽  
pp. 77-112 ◽  
Author(s):  
LUIS M. P. CERÍACO ◽  
MATTHEW P. HEINICKE ◽  
KELLY L. PARKER ◽  
MARIANA P. MARQUES ◽  
AARON M. BAUER

The genus Panaspis in Angola is represented by four species, most of them part of taxonomically and nomenclaturally challenging species-complexes. We present a taxonomic revision of the group in the region and describe one new species, Panaspis mocamedensis sp. nov., endemic to the lowland areas of the Namibe province, southwestern Angola. Phylogenetic analysis using a combination of mitochondrial (16S, cytb) and nucleares (RAG1, PDC) markers, as well as morphological and meristic data support the recognition of the new species. In addition, these data support the presence of nominotypical Panaspis cabindae, P. wahlbergi and P. maculicollis in Angola. Reexamination of the Angolan population of P. breviceps was based on morphological analysis, as no molecular data from Angola is available for this species. According to our results, this population likely represents the nominotypical form, but due to its complex taxonomic and nomenclatural history and the lack of molecular data, this population needs to be reconsidered when molecular data become available. The description of a new species and revision of the Angolan Panaspis contributes to a better understanding of the true species richness of the Angolan herpetofauna, as well as to understanding the major biogeographic patterns of the region. A key to Angolan Panaspis species is also presented. 


2018 ◽  
Vol 61 (2) ◽  
pp. 127-140 ◽  
Author(s):  
Beatriz N. Torrano-Silva ◽  
Bruno R. Vieira ◽  
Rafael Riosmena-Rodríguez ◽  
Mariana C. Oliveira

AbstractMultiple-marker (COI-5P, UPA,psbA andrbcL-3P) and two algorithmic approaches [automatic barcode gap discovery (ABGD) and Poisson tree process (PTP)] were used for species delimitation of Lithophylloideae in Brazil. The integrative approach was mostly congruent between markers and algorithmic methods of species delimitation, suggesting the occurrence of 24 species. Based on morphology and molecular data,Amphiroa rigida,Amphiroa vanbosseae,Lithophyllum atlanticum,Lithophyllum kaiseri,Lithophyllum margaritae,Titanoderma pustulatum,Titanoderma prototypumandPaulsilvella huveorum, which were previously reported for Brazil, are confirmed in this work. Six new species are distinguished by both molecular and morphological traits, and they are provisionally named asAmphiroasp. 1,Amphiroasp. 2,Amphiroasp. 3,Lithophyllumsp. 1,Lithophyllumsp. 2 andLithophyllumsp. 3. Another 10 species are cryptic and cannot be distinguished based on traditionally used morphological traits. These includeAmphiroasp. 4,Lithophyllumsp. 4, three species that are morphologically named underAmphiroa beauvoisii, and six that share the morphology described forAmphiroa fragilissima. All four markers used were useful for species delimitation. However, a combination of practical aspects and levels of intra- and interspecific divergence values led us to propose the use ofrbcL-3P as a standard DNA barcode marker for the Corallinales.


Zootaxa ◽  
2018 ◽  
Vol 4446 (1) ◽  
pp. 81 ◽  
Author(s):  
HANNA R. ROYALS ◽  
JEAN-FRANÇOIS LANDRY ◽  
TODD M. GILLIGAN

The genus Paralobesia Obraztsov, 1953 is found primarily in eastern North America and consists of 18 described and several undescribed species. Prior to 1900, all North American Paralobesia were assumed to be P. viteana (Clemens). However, rearing experiments by William Kearfott in the early 1900s suggested that species of Paralobesia were monophagous and could be separated by host. Recently, a species of Paralobesia was reared from showy lady’s slipper, Cypripedium reginae Walter (Orchidaceae), during a study of two populations of this orchid in eastern Ontario and southwestern Québec. Although originally assumed to be P. cypripediana (Forbes), which was described from specimens reared from Cypripedium in Manitoba, DNA barcode data and genital morphology confirmed that this was a new species similar to P. cypripediana and P. monotropana (Heinrich). Herein, we describe P. marilynae, sp. n., and provide specifics of its discovery and life history. Rearing records indicate that Paralobesia can span the range from strictly monophagous to polyphagous, even for very similar species with similar feeding habits, and that host records should be combined with morphological and molecular data when circumscribing species in this genus. This work is part of a complete systematic revision of Paralobesia currently in progress. 


Zootaxa ◽  
2011 ◽  
Vol 3147 (1) ◽  
pp. 1 ◽  
Author(s):  
ANNEMARIE OHLER ◽  
KATHARINA C. WOLLENBERG ◽  
STÉPHANE GROSJEAN ◽  
RALF HENDRIX ◽  
MIGUEL VENCES ◽  
...  

Frogs in the subgenus Lalos of the genus Leptolalax (Megophryidae) are highly diversified in continental Asia and consist of about 17 nominal species. These frogs are small, inconspicuous, and of high superficial morphological similarity.        We here formulate a hypothesis of phylogenetic relationships and assess the amount of genetic variation among genealogical lineages on the basis of 536bp of mitochondrial 16S rDNA sequences. Combining molecular data with a study of morpho-logy, morphometric divergence and geographical proximity, we tested hypotheses of species identity. We (1) used character-based and morphometric analyses to assign the onymophoronts (type specimens) of species in Lalos available to us to respectively one of the main clades, in order to propose the best potential correct taxonomic and nomenclatural allocation for the individuals included in the molecular study, and (2) tried to also assign the historical museum specimens to these molecular taxonomic units and to reclassify them whenever necessary.        We also used the molecular data to match tadpoles with adults and provide tadpole descriptions for species the larvae of which were previously unknown. Specimens, that could neither be allocated to a molecularly characterised species (on the basis of their DNA “barcode”) nor to a morphologically defined species named on the basis of a type specimen, are described here as new species. Based on this integrative set of data and analyses we describe two new species, Leptolalax eos n. sp. and Leptolalax nyx n. sp., we resurrect Leptolalax minimus, and reassess the distribution of the species studied. We propose changes in the Red List status of L. pelodytoides and L. ventripunctatus and suggest a conservation status for the new species described herein.


Zootaxa ◽  
2017 ◽  
Vol 4226 (2) ◽  
pp. 151 ◽  
Author(s):  
CLAUDIA HEMP

A checklist of Ensifera and Acridomorpha of Kazimzumbwi Forest Reserve, Kisarawe near Dar es Salaam is given and eight new Tettigoniidae species described. These are the Agraeciini species Afroagraecia kisarawe n. sp., the Meconematinae species Phlugidia kisarawe n. sp., and the female of Aerotegmina megaloptera (Hexacentrinae). The Phaneropterinae species Dioncomena scutellata n. sp. is known at present only from two localities, the Pugu Hills near Dar es Salaam and Kwamgumi forest reseve on the foothills of the East Usambara Mountains. Two new Eurycorypha species, E. annexata n. sp. and E. ligata n. sp. are described from the area known at present only from the male sex. A second species is described in the genus Lunidia Hemp, L. acuticercata n. sp. Two new Phaneropterinae genera are erected on Pseudopreussia flavifolia n. gen. n. sp. and Materuana ericki n. gen. n. sp., species of wet lowland forest along coastal Tanzania and forest reserves in the East Usambara and on the foothills of the Uluguru Mountains.Kazimzumbwi Forest Reserve is severly threatened by encroachment and deforestation although it is recognized as belonging to the oldest surviving forests of the world. 


2021 ◽  
Vol 157 (2) ◽  
pp. 109-145
Author(s):  
H. Charles ◽  
J. Godfray

An annotated checklist of the British Dacnusini and the Dapsilarthra genus group of the Alysiini (Hymenoptera: Braconidae, Alysiinae) is provided. In addition to a species list with synonymy (based on Broad, Shaw & Godfray 2016) further details are given of: (i) intrageneric groupings; (ii) a reference to each species' treatment in the unindexed and multipart major revisions of Nixon & Griffith; (iii) a hypothesis about host range, where known (about three quarters of species), and the evidence upon which this is based; (iv) DNA barcode BIN references where available (currently for about half the species); (v) any further relevant notes. Brief mention is made of 10 likely new species which require further material before they can be described. 23 new 'nation' records (i.e. for Scotland, Wales etc.) and 29 new parasitoid-host associations are recorded.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5867 ◽  
Author(s):  
Olivia Messinger Carril ◽  
Terry Griswold ◽  
James Haefner ◽  
Joseph S. Wilson

Interest in bees has grown dramatically in recent years in light of several studies that have reported widespread declines in bees and other pollinators. Investigating declines in wild bees can be difficult, however, due to the lack of faunal surveys that provide baseline data of bee richness and diversity. Protected lands such as national monuments and national parks can provide unique opportunities to learn about and monitor bee populations dynamics in a natural setting because the opportunity for large-scale changes to the landscape are reduced compared to unprotected lands. Here we report on a 4-year study of bees in Grand Staircase-Escalante National Monument (GSENM), found in southern Utah, USA. Using opportunistic collecting and a series of standardized plots, we collected bees throughout the six-month flowering season for four consecutive years. In total, 660 bee species are now known from the area, across 55 genera, and including 49 new species. Two genera not previously known to occur in the state of Utah were discovered, as well as 16 new species records for the state. Bees include ground-nesters, cavity- and twig-nesters, cleptoparasites, narrow specialists, generalists, solitary, and social species. The bee fauna reached peak diversity each spring, but also experienced a second peak in diversity in late summer, following monsoonal rains. The majority of GSENM’s bees are highly localized, occurring in only a few locations throughout the monument, and often in low abundance, but consistently across the four years. Only a few species are widespread and super-abundant. Certain flowering plants appear to be inordinately attractive to the bees in GSENM, including several invasive species. GSENM protects one of the richest bee faunas in the west; the large elevational gradient, incredible number of flowering plants, and the mosaic of habitats are all likely contributors to this rich assemblage of bees.


Zootaxa ◽  
2019 ◽  
Vol 4691 (2) ◽  
pp. 139-152
Author(s):  
DENGQING LI ◽  
HAO LIU ◽  
XIN XU

We diagnose and describe two Songthela and one Vinathela species of the primitively segmented spiders, Liphistiidae, as new to science based on morphological characters and molecular data: Songthela pyriformis sp. nov. (male, female), Songthela shuyuan sp. nov. (male, female), Vinathela nenglianggu sp. nov. (male, female), which were collected at Mt Yuelu in Changsha, Hunan Province, China. To facilitate future identification of our species, we also provide the GenBank accession numbers of the DNA barcode gene, cytochrome c oxidase subunit I (COI) for all type specimens and the evidence of genetic distances based on COI for three new species. 


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