scholarly journals Genital anatomy, jaw and radula of Guladentia subtussulcata (Helicoidea, Cepolidae), endemic to western Cuba

ZooKeys ◽  
2022 ◽  
Vol 1080 ◽  
pp. 99-106
Author(s):  
Maike Hernández ◽  
Manuel A. Bauzá ◽  
Thierry Backeljau
Keyword(s):  
Copulatory Organ ◽  
Accessory Gland ◽  
Entire Surface ◽  
Mucus Gland

This study provides the first data on the genital anatomy, jaw and radula of Guladentia subtussulcata (L. Pfeiffer, 1863). The auxiliary copulatory organ of this species is very peculiar, similar to that of Jeanneretia L. Pfeiffer, 1877, and different from that of other cepolids. It consists of an elongate, pedunculate mucus gland inserted apically on a muscular papilla and an atrial sac, all covered by a sheath. A sheath-like accessory gland is inserted at the base of the atrial sac. Another similarity with Jeanneretia is the presence of a fertilization pouch-spermatheca complex with a single exposed spermatheca. Like Jeanneretia, G. subtussulcata has an oxygnath, highly arched jaw with slight striae over the entire surface and a broad, well-developed median projection. The radula has triangular and monocuspid central and lateral teeth (the central teeth are smaller than the rest). The marginal teeth are multicuspid with the mesocone and ectocones smaller than the endocones. The similar structures of the auxiliary copulatory organ (without dart sac) and spermatheca (simple) strongly suggest that G. subtussulcata and Jeanneretia spp. are closely related. As such, it remains to be decided whether Guladentia Clench & Aguayo, 1951 and Jeanneretia should continue to be treated as separate genera.

Inhibition Effect Extract Stolephorus insularis as a Antimicroba in Staphylococcus aereus Bacteria

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 22
Author(s):  
Ayu Listya ◽  
Yulie Emilda Akhwan
Keyword(s):  
Staphylococcus Aureus ◽  
Root Canal ◽  
Quantitative Research ◽  
Media Culture ◽  
Root Canal Treatment ◽  
Research Needs ◽  
Dmso Solution ◽  
Entire Surface ◽  
Inhibition Effect ◽  
Inhibitory Power

<pre><em><strong>Background :</strong> U</em><em>ntreated caries will continue and microorganisms enter the pulp resulting in an inflammatory response and pulp necrosis. Sterilization is one part of root canal treatment and is important for the success of root canal treatment. Stolephorus insularis contains antibacterial substances such as fluor. Previous research showed that extract Stolephorus insularis has antibacterial activity in Streptococcus mutans bacteria.</em><em></em></pre><pre><em>Purpose : T</em><em>his study aims to determine the antibacterial power of Stolephorus insularis extract on the growth of Staphylococcus aereus bacteria by using 3 concentrations of 18%, 24% and 30%.</em></pre><pre><em>Material and Method :</em><em>This resesarch use culture of Staphylococcus aereus bacteria in MH blood agar, Stolephorus insularis extract with various concentrations (18%, 24%, 30%), 7% Na CMC, 1% DMSO solution, agar BHI media. Culture of Staphylococcus aereus was taken from liquid BHI media, then rubbed on the entire surface of BHI media using a sterile cotton stick. The antibacterial zone formed is measured.</em></pre><pre> </pre><pre><em>Result : B</em><em>ased on the results of the Mann-Whitney test there were significant differences in the inhibitory power of Staphylococcus aureus bacteria (p &lt;0.05) in the K + group with groups K-, P1, P2 and P3. Whereas between groups P1 with K-, group P2 with K- and P1, and group P3 with K-, P1 and P2 there were no significant differences because the significant value was greater than 0.05</em><em></em></pre><pre><em>Conclusion : T</em><em>here is no antimicrobial power at concentrations of 18%, 24%, and 30%. Quantitative research needs to be done to determine the decrease in the number of bacterial colonies.</em><em></em></pre><p><em><br /></em></p><p><em><strong>Keywords :</strong> Inhibition effect, Stolephorus insularis, Staphylococcus aereus</em></p><p><em><br /></em></p><p><em><strong>Correspondence :</strong> </em><em>Ayulistya Paramita, Paediatric Departemen. FKG UHT, Jl Arief Rahman Hakim no 150 Surabaya. email : [email protected]</em></p>

Enumeration of Cryptosporidium oocysts from surface water concentrates by laser-scanning cytometry

2000 ◽  
Vol 41 (7) ◽  
pp. 197-202 ◽  
Author(s):  
F. Zanelli ◽  
B. Compagnon ◽  
J. C. Joret ◽  
M. R. de Roubin
Keyword(s):  
Surface Water ◽  
Water Samples ◽  
Laser Scanning ◽  
Immunomagnetic Separation ◽  
Entire Surface ◽  
Cryptosporidium Oocysts ◽  
Different Types ◽  
Laser Scanning Cytometry ◽  
Direct Microscopic Observation

The utilization of the ChemScan® RDI was tested for different types of water concentrates. Concentrates were prepared by cartridge filtration or flocculation, and analysed either without purification, or after Immunomagnetic separation (IMS) or flotation on percoll-sucrose gradients. Theenumeration of the oocysts was subsequently performed using the ChemScan® RDI Cryptosporidium application. Enumeration by direct microscopic observation of the entire surface of the membrane was carried out as a control, and recoveries were calculated as a ratio between the ChemScan® RDI result and the result obtained with direct microscopic enumeration. The Chemscan enumeration technique proved reliable, with recoveries yielding close to 100% in most cases (average 125%, range from 86 to 467%) for all the concentration/purification techniques tested. The quality of the antibodies was shown to be critical, with antibodies from some suppliers yielding recoveries a low as 10% in some cases. This difficulty could, however, be overcome by the utilization of the antibody provided by Chemunex. These data conclusively prove that laser scanning cytometry, which greatly facilitates the microscopic enumeration of Cryptosporidium oocysts from water samples and decreases the time of observation by four to six times, can be successfully applied to water concentrates prepared from a variety of concentration/purification techniques.

Female-Limited Polymorphism in the Copulatory Organ of a Traumatically Inseminating Insect

2007 ◽  
Vol 170 (6) ◽  
pp. 931
Author(s):  
Reinhardt ◽  
Ewan Harney ◽  
Naylor ◽  
Gorb ◽  
Siva-Jothy
Keyword(s):  
Copulatory Organ

scholarly journals Development of a Mucus Gland Bioreactor in Loach Paramisgurnus dabryanus

2021 ◽  
Vol 22 (2) ◽  
pp. 687
Author(s):  
Tong Zhou ◽  
Bolan Zhou ◽  
Yasong Zhao ◽  
Qing Li ◽  
Guili Song ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Grass Carp ◽  
Single Copy ◽  
Type I ◽  
Western Blots ◽  
Paramisgurnus Dabryanus ◽  
Expression Activity ◽  
Transgenic Construct ◽  
Mucus Gland

Most currently available bioreactors have some defects in the expression, activity, or purification of target protein and peptide molecules, whereas the mucus gland of fish can overcome these defects to become a novel bioreactor for the biopharmaceutical industry. In this study, we have evaluated the practicability of developing a mucus gland bioreactor in loach (Paramisgurnus dabryanus). A transgenic construct pT2-krt8-IFN1 was obtained by subcloning the promoter of zebrafish keratin 8 gene and the type I interferon (IFN1) cDNA of grass carp into the SB transposon. The IFN1 expressed in CIK cells exhibited an antiviral activity against the replication of GCRV873 and activated two genes downstream of JAK-STAT signaling pathway. A transgenic loach line was then generated by microinjection of the pT2-krt8-IFN1 plasmids and in vitro synthesized capped SB11 mRNA. Southern blots indicated that a single copy of IFN1 gene was stably integrated into the genome of transgenic loach. The expression of grass carp IFN1 in transgenic loaches was detected with RT-PCR and Western blots. About 0.0825 µg of grass carp IFN1 was detected in 20 µL mucus from transgenic loaches. At a viral titer of 1 × 103 PFU/mL, plaque numbers on plates containing mucus from transgenic loaches reduced by 18% in comparison with those of the control, indicating that mucus of IFN1-transgenic loaches exhibited an antiviral activity. Thus, we have successfully created a mucus gland bioreactor that has great potential for the production of various proteins and peptides.

Offsetting Effects of Wolbachia Infection and Heat Shock on Sperm Production inDrosophila simulans: Analyses of Fecundity, Fertility and Accessory Gland Proteins

Genetics ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 167-178 ◽  
Author(s):  
Rhonda R Snook ◽  
Sophia Y Cleland ◽  
Mariana F Wolfner ◽  
Timothy L Karr
Keyword(s):  
Heat Shock ◽  
Natural Populations ◽  
Adult Life ◽  
Reproductive Factors ◽  
Male Gamete ◽  
Wolbachia Infection ◽  
Accessory Gland ◽  
Sperm Production ◽  
Accessory Gland Proteins ◽  
Mating Conditions

AbstractInfection in Drosophila simulans with the endocellular symbiont Wolbachia pipientis results in egg lethality caused by failure to properly initiate diploid development (cytoplasmic incompatibility, CI). The relationship between Wolbachia infection and reproductive factors influencing male fitness has not been well examined. Here we compare infected and uninfected strains of D. simulans for (1) sperm production, (2) male fertility, and (3) the transfer and processing of two accessory gland proteins, Acp26Aa or Acp36De. Infected males produced significantly fewer sperm cysts than uninfected males over the first 10 days of adult life, and infected males, under varied mating conditions, had lower fertility compared to uninfected males. This fertility effect was due to neither differences between infected and uninfected males in the transfer and subsequent processing of accessory gland proteins by females nor to the presence of Wolbachia in mature sperm. We found that heat shock, which is known to decrease CI expression, increases sperm production to a greater extent in infected compared to uninfected males, suggesting a possible link between sperm production and heat shock. Given these results, the roles Wolbachia and heat shock play in mediating male gamete production may be important parameters for understanding the dynamics of infection in natural populations.

scholarly journals Accuracy Verification of an Anatomical Model Manufactured Using Low-Cost Additive Production

2021 ◽  
Vol 11 (2) ◽  
pp. 594
Author(s):  
Teodor Tóth ◽  
Patrik Varga ◽  
Branko Štefanovič ◽  
Lucia Bednarčíková ◽  
Marek Schnitzer ◽  
...  
Keyword(s):  
Surface Coverage ◽  
Reference Model ◽  
Low Cost ◽  
Cervical Vertebra ◽  
3D Printer ◽  
3D Scanner ◽  
Entire Surface ◽  
Additive Production ◽  
The Third ◽  
And Training

The paper deals with the separation of the third cervical vertebra using the software VGStudio MAX, Mimics, and inVesalius. During the separation, various parameters of the threshold were used to determine the effect. The comparison of models from Mimics and inVesalius to VGStudio MAX showed that the cumulative variance distribution for 95% surface coverage is less than 0.935 mm. When comparing medically oriented software, Mimics and inVesalius, the deviation was less than 0.356 mm. The model was made of polylactic acid (PLA) material on a low-cost 3D printer, Prusa i3 MK2.5 MMU1. The printed model was scanned by four scanners: Artec Eva, 3Shape D700, Steinbichler Comet L3D, and Creaform EXAscan. The outputs from the scanners were compared to the reference model (standard tessellation language (STL) model for 3D printing) as well as to the scanner with the best accuracy (3Shape). Compared to the publications below, the analysis of deviations was evaluated on the entire surface of the model and not on selected dimensions. The cumulative variance distribution for comparing the output from the 3D scanner with the reference model, as well as comparing the scanners, shows that the deviation for 95% of the surface coverage is at the level of 0.300 mm. Since the model of the vertebra is planned for education and training, the used software and technologies are suitable for use in the design and the production process.

Autogeny in saltmarsh mosquitoes induced by a substance from the male accessory gland

Nature ◽  
1977 ◽  
Vol 267 (5609) ◽  
pp. 342-344 ◽  
Author(s):  
GEORGE F. O'MEARA ◽  
DAVID G. EVANS
Keyword(s):  
Accessory Gland ◽  
Male Accessory Gland

scholarly journals A NOTE ON THE PROGRESS OF CICATRIZATION OF WAR WOUNDS

1918 ◽  
Vol 27 (2) ◽  
pp. 165-178
Author(s):  
Theodore Tuffler ◽  
R. Desmarres
Keyword(s):  
Epithelial Proliferation ◽  
Entire Surface ◽  
Chemical Substances ◽  
War Wounds ◽  
Arterial Circulation ◽  
Calculated Curve ◽  
Biologic Process ◽  
Scalp Wound ◽  
Lost Time ◽  
Organic Fluids

Some hypothetical conclusions bearing on the evolution of cicatricial tissue can be suggested. The arterial circulation deposits in the wound chemical substances necessary for contraction of the wound and for epithelial proliferation. When the biologic process is not hindered by any special or severe bacterial infection this deposit is as regular as the circulation itself, and enables us to determine in advance the date of cicatrization. It even seems as though when the epidermization process is retarded by a slight infection the substances necessary for epidermization are stored up in the wound, and when the delay due to infection is removed the epithelium finds an accumulation of nutritive substances, and, so to speak, makes up the lost time. Moreover, when an infection entirely or partially stops epidermization, we have observed (Experiments 3, 4, and 5) that after the infection has disappeared the progress of new epidermization is much more rapid than normally; it even passes the calculated curve. The infection apparently destroyed only the epithelium and left in the wound the chemical substances which activate epidermization. The existence of these physical or chemical activating agents has been indicated again by two anatomical clinical facts. In treating a scalp wound in which there had been practically no epidermization for many months, we applied over the entire surface of the sterile wound dermo-epidermic grafts of fetal skin. After apparently taking, the grafts were absorbed and disappeared, but epidermization of the periphery of the wound, which hitherto had not progressed, took place abundantly, almost a hundred times as much as before. We believe that by mathematical measurements we can solve the problem of the action of various organic fluids on the cicatrization of wounds.

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