Antioxidant activity optimisation of Spirulina platensis C-phycocyanin obtained by freeze-thaw, microwave-assisted and ultrasound-assisted extraction methods

2017 ◽  
Vol 9 (1) ◽  
pp. 1-9 ◽  
Author(s):  
R. Vali Aftari ◽  
K. Rezaei ◽  
A.R. Bandani ◽  
A. Mortazavi
Keyword(s):  
Antioxidant Activity ◽  
Spirulina Platensis ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Freeze Thaw ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals EKSTRAKSI FIKOSIANIN DARI SPIRULINA PLANTESIS SEBAGAI BIOPIGMEN DAN ANTIOKSIDAN

2017 ◽  
Vol 8 (1) ◽  
pp. 36
Author(s):  
Siti Irma Rahmawati ◽  
Syarif Hidayatullah ◽  
Mira Suprayatmi
Keyword(s):  
Human Body ◽  
Spirulina Platensis ◽  
Extraction Method ◽  
Antioxidant Activities ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Freeze Thaw ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Better Than

Pewarna alami lebih disukai karena tidak memiliki efek yang negatif terhadap tubuh manusia sehingga mempunyai tingkat keamanan pangan yang tinggi, selain itu juga pewarna alami mudah diuraikan. Bahan pewarna alami bisa diperoleh dari berbagai jenis sumber, salah satunya yaitu spesies alga yang merupakan tumbuhan tingkat rendah di perairan. Alga terdapat 2 jenis yaitu makroalga yang berukuran besar dan mikroalga yang berukuran kecil (renik). Spesies mikroalga yang dapat menghasilkan pewarna alami salah satunya adalah Spirulina. Jenis pewarna alami yang terkandung pada mikroalga tersebut yaitu fikosianin. Pada penelitian ini, dilakukan ekstraksi fikosianin dari Spirulina platensis dengan menggunakan tiga metode berbeda yaitu Maserasi, Ultrasound-Assisted-Extraction (UAE) dan Freezing untuk mengetahui metode mana untuk menghasilkan yield dan antioksidan tertinggi dari ekstrak yang dihasilkan. Hasil dari penelitian ini didapatkan bahwa berdasarkan nilai yield tertinggi dan kandungan fikosianin, freezing merupakan metode ekstraksi terbaik dengan kandungan fikosianin yang paling tinggi yaitu sebesar 26,53%. Sedangkan nilai aktivitas antioksidan terbesar dihasilkan oleh metode ekstraksi maserasi dengan nilai IC50 sebesar 49,59 ppm. Fikosianin yang dihasilkan dari ketiga metode ekstraksi termasuk antioksidan kuat.KATA KUNCI: ekstraksi, Spirulina plantesis, fikosianin, biopigmen, antioksidanEXTRACTION OF PHYCOCYANIN FROM SPIRULINA PLANTESIS FOR BIOPIGMENT AND ANTIOXIDANTABSTRACTRecently, biopigment known as a pigment which has no impact and easy to degradated in human body and better than artificial pigment. Biopigment can be resulted from the extraction of algae that are living in the water. Spirulina plantesis is one of algae resulting a pigment and known as phycocyanin. In this research, extraction of phycocyanin was done by three method of extractions; maceration, Ultrasound-Assisted-Extraction (UAE) and Freeze-thaw. Those extraction methods were compared to decide which method that resulted highest yield of extraction and has highest antioxidant activities. From the resulted data, the highest yield and highest phycocyanin concentration was obtained by freeze-thaw extraction method. Phycocyanin concentration from freeze-thaw ectract was 26,53% (w/w). However, the highest antioxidant activities was obtained by maceraion extraction mehod. The IC50 of extract from maceration was 49,59 ppm. Hence, all extracts from different extraction methods resulted high antioxidant activities. 

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

scholarly journals Simultaneous Determination of REEs in Coal Samples Using the Combination of Microwave-Assisted Ashing and Ultrasound-Assisted Extraction Methods Followed by ICP-OES Analysis

Minerals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1103
Author(s):  
Mceliseni Zuma ◽  
Philiswa Nomngongo ◽  
Nomvano Mketo
Keyword(s):  
Inductively Coupled Plasma ◽  
Bath Temperature ◽  
Extraction Methods ◽  
Icp Oes ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Ultrasonic Bath ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The world during the COVID-19 pandemic has led to extensive use of virtual activities by means of electronic devices, which are made up of rare earth elements (REEs). This means that quantitative knowledge of REEs in various resources is crucial for the development of effective recovery methods. Therefore, this report describes a simple microwave assisted ashing followed by ultrasound-assisted extraction (MAA-UAE) for quantitative determination of REEs in coal samples using inductively coupled plasma-optical emission spectroscopy (ICP-OES). Firstly, coal samples were ashed at 55 °C for 4.5 h to form white ashes, which were then treated with dilute HNO3 acid under ultrasonication to enhance the extraction of REEs. The quantitative recoveries (86–120%) of REEs were obtained when 1 mol L−1, 0.1 g, 40 °C, 20 min, and high frequency were applied for [HNO3], sample mass, ultrasonic bath temperature, extraction time, and ultrasonic bath frequency, respectively. The method detection limits of the proposed MAA-UAE method were between 0.0075 and 0.59 µg g−1 with satisfactory precision (<5%). The concentration levels of REEs in South African coals ranged from 1.4 to 105 µg g−1, suggesting that this coal can be a resource for REEs.

scholarly journals Ziziphus lotus (L.) Lam. plant treatment by ultrasounds and microwaves to improve antioxidants yield and quality: An overview

2021 ◽  
Vol 5 (12) ◽  
pp. 53-68
Author(s):  
Farida Berkani ◽  
Maria Luísa Serralheiro ◽  
Farid Dahmoune ◽  
Malik Mahdjoub ◽  
Nabil Kadri ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Yield ◽  
Point Of View ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Yield And Quality ◽  
Assisted Extraction ◽  
Industrialization Process ◽  
Ultrasound Assisted

The purpose of this review is to compile the literature published about different aspects of microwave-assisted extraction (MAE) use and ultrasound-assisted extraction (UAE) applied on jujube worldwide and to compare the results on the antioxidant activity obtained for each extraction method. As a result of the increased consumers demand for natural products, as well as for those of agro-food, nutraceutical, cosmetic industries, and green extraction techniques are nowadays trending to be potential alternatives that can improve antioxidant yield and its quality from an economical and environmental point of view by reducing time, energy, and solvent consumption. Ultrasounds and microwaves are widely used methods in the extraction of active principles due to their cavitation and dipolar rotation effect, respectively. These two techniques provide efficiency of extraction while minimizing the time and preserving the quality of the food matrix, overcoming the disadvantages of conventional techniques characterized by their consumption of large quantities of solvents and providing a sparse quantity of extraction. Jujube, a shrub with a high antioxidant potential, which can be affected by various extraction conditions can be the target of UAE and MAE to increase the antioxidant extraction yield. Exploiting the beneficial properties such as the antioxidant activity can lead to an industrialization process, replacing therefor synthetic antioxidants with natural compounds. These can also help in the development of new nutraceuticals and can be used, for instance, in agro-food industries as preservatives. Keywords : Microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), antioxidants, Ziziphus lotus (L.) Lam plant.

scholarly journals Flavonol Composition and Antioxidant Activity of Onions (Allium cepa L.) Based on the Development of New Analytical Ultrasound-Assisted Extraction Methods

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 273
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Estrella Espada-Bellido ◽  
Ceferino Carrera ◽  
Marta Ferreiro-González ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Volume Ratio ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Quercetin Derivatives ◽  
Extraction Solvent ◽  
Assisted Extraction ◽  
Response Optimization ◽  
Ultrasound Assisted

The onion is one of the most cultivated and consumed vegetables, and is a very valuable source of antioxidant substances. Every onion variety is rich in flavonols—mainly quercetin derivatives which makes onions the main dietary source of these compounds. This study intends to develop an ultrasound-assisted extraction method (UAE), an economical, green, and efficient technique, that allows us to determine the quality of onion extracts in terms of flavonol composition and antioxidant activity. For this purpose, an ultrasound-assisted extraction method has been optimized to obtain extracts with a high amount of flavonols, as well as with a high antioxidant activity, not only separately using a Box–Behnken design, but also simultaneously, based on multi-response optimization. Multiple response optimization has not only demonstrated an efficacy level similar to that of the individual ones, but it also represents a considerable reduction in terms of cost, time and work. The optimal conditions for simultaneous extractions were determined as follows: 76.8% methanol as an extraction solvent at pH 2 and 58.5 °C temperature, 85% amplitude, 0.9 s cycle, and 0.2:13 g:mL sample mass/solvent volume ratio. Furthermore, the developed method exhibited a high precision level and great recoveries in a rather short extraction time (2 min). These results, together with the development of a fast and simple UHPLC analysis method, lead us to consider the developed UAE method as a suitable technique for the extraction of bioactive compounds from onion matrices.

scholarly journals Extraction Methods for the Isolation of Isoflavonoids from Plant Material

2017 ◽  
Vol 15 (1) ◽  
pp. 34-45 ◽  
Author(s):  
Tomasz Blicharski ◽  
Anna Oniszczuk
Keyword(s):  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Phytochemical Analysis ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction ◽  
Pharmacological Activities ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Biological And Pharmacological Activities

AbstractThe purpose of this review is to describe and compare selected traditional and modern extraction methods employed in the isolation of isoflavonoids from plants. Conventional methods such as maceration, percolation, or Soxhlet extraction are still frequently used in phytochemical analysis. Despite their flexibility, traditional extraction techniques have significant drawbacks, including the need for a significant investment of time, energy, and starting material, and a requirement for large amounts of potentially toxic solvents. Moreover, these techniques are difficult to automate, produce considerable amount of waste and pose a risk of degradation of thermolabile compounds. Modern extraction methods, such as: ultrasound-assisted extraction, microwave-assisted extraction, accelerated solvent extraction, supercritical fluid extraction, and negative pressure cavitation extraction, can be regarded as remedies for the aforementioned problems. This manuscript discusses the use of the most relevant extraction techniques in the process of isolation of isoflavonoids, secondary metabolites that have been found to have a plethora of biological and pharmacological activities.

scholarly journals Application And Comparison Of Different Extraction Methods of capsaicin from chili, Capsicum annuum L.

2016 ◽  
Vol 19 (2) ◽  
pp. 44-51
Author(s):  
Khoa Anh Tran ◽  
Tuyet Thi Ngoc Nguyen ◽  
Phung Thi Kim Le
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Extraction Method ◽  
Extraction Methods ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Main Component

The extraction of chili oil and capsaicin from chili using some different method such as Soxhlet, maceration, ultrasound-assisted extraction, microwave-assisted extraction and supercritical carbon dioxide with ethanol as a modifier was studied in this work. The results showed that the main component of chili oil is polyunsaturated fatty acids (representing 77.4%); mainly oleic acid and linoleic acid. The highest yield of oil and capsaicin were 0,5358g/g and 2,9534mg/g material; achieved from microwave-assisted extraction method. However, the highest selectivity was 1.77% with supercritical fluid extraction method. With additional co-solvent, the concentration of capsaicin increased but the selectivity decreased.

scholarly journals Ziziphus lotus (L.) Lam. plant treatment by ultrasounds and microwaves to improve antioxidants yield and quality: An overview

2021 ◽  
Vol 5 (12) ◽  
pp. 53-68
Author(s):  
Farida Berkani ◽  
Maria Luísa Serralheiro ◽  
Farid Dahmoune ◽  
Malik Mahdjoub ◽  
Nabil Kadri ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Yield ◽  
Point Of View ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Yield And Quality ◽  
Assisted Extraction ◽  
Industrialization Process ◽  
Ultrasound Assisted

The purpose of this review is to compile the literature published about different aspects of microwave-assisted extraction (MAE) use and ultrasound-assisted extraction (UAE) applied on jujube worldwide and to compare the results on the antioxidant activity obtained for each extraction method. As a result of the increased consumers demand for natural products, as well as for those of agro-food, nutraceutical, cosmetic industries, and green extraction techniques are nowadays trending to be potential alternatives that can improve antioxidant yield and its quality from an economical and environmental point of view by reducing time, energy, and solvent consumption. Ultrasounds and microwaves are widely used methods in the extraction of active principles due to their cavitation and dipolar rotation effect, respectively. These two techniques provide efficiency of extraction while minimizing the time and preserving the quality of the food matrix, overcoming the disadvantages of conventional techniques characterized by their consumption of large quantities of solvents and providing a sparse quantity of extraction. Jujube, a shrub with a high antioxidant potential, which can be affected by various extraction conditions can be the target of UAE and MAE to increase the antioxidant extraction yield. Exploiting the beneficial properties such as the antioxidant activity can lead to an industrialization process, replacing therefor synthetic antioxidants with natural compounds. These can also help in the development of new nutraceuticals and can be used, for instance, in agro-food industries as preservatives. Keywords : Microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), antioxidants, Ziziphus lotus (L.) Lam plant.

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