Fumonisin B1-4-producing capacity of Hungarian Fusarium verticillioides isolates

2010 ◽  
Vol 3 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Á. Szécsi ◽  
A. Szekeres ◽  
T. Bartók ◽  
G. Oros ◽  
M. Bartók ◽  
...  
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Cultural Characteristics ◽  
The Third ◽  
Rice Grains ◽  
Rp Hplc ◽  
Culture Material ◽  
Analytical Results ◽  
Different Sources

The fumonisin B1-4 (FB1-4) toxin-producing capacities of 60 strains of Fusarium verticillioides isolated in the main maize-cultivating areas of Hungary were screened on rice grains in vitro. The amounts of FB1, FB2, FB3 and FB4 in the extracts of the culture material were determined by RP-HPLC/ESI-IT MS without any sample clean-up. All F. verticillioides strains produced all analogues (FB1-4) of the fumonisin B series. The strains did not differ significantly in their cultural characteristics, though the analytical results allowed the distinction of three idiosyncratic FB1-4-producing chemotypes with characteristic proportions of the fumonisin B analogues. The dominant chemotype produced them in the sequence FB1 > FB2 > FB3 > FB4, with large amounts of FB1 and FB2. A second chemotype produced a higher amount of FB3 than of FB2, while the third chemotype produced large amounts of FB2 and FB4. The proportion of FB1 in the extracts was inversely correlated with the total FB yield. No differences in FB1-4 producing capacity were observed between strains isolated from various locations or different sources.

scholarly journals In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries

2020 ◽  
Vol 8 (2) ◽  
pp. 241 ◽  
Author(s):  
Giovanni Beccari ◽  
Łukasz Stępień ◽  
Andrea Onofri ◽  
Veronica M. T. Lattanzio ◽  
Biancamaria Ciasca ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Geographical Origin ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Genetic Profile ◽  
Mediterranean Countries ◽  
Intergenic Regions ◽  
Fumonisin B2 ◽  
High Level

Investigating the in vitro fumonisin biosynthesis and the genetic structure of Fusarium verticillioides populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 F. verticillioides strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B1, fumonisin B2 and fumonisin B3 and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03–69.84 μg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent FUM1 gene and of intergenic regions FUM6-FUM7 and FUM7-FUM8 were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains.

scholarly journals Immunobiological Effects of Fumonisin B1 in Experimental Subchronic Mycotoxicoses in Rats

2002 ◽  
Vol 9 (1) ◽  
pp. 149-155 ◽  
Author(s):  
M. G. Theumer ◽  
A. G. López ◽  
D. T. Masih ◽  
S. N. Chulze ◽  
H. R. Rubinstein
Keyword(s):  
Body Weight ◽  
Small Intestine ◽  
Mononuclear Cells ◽  
Body Weight Gain ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Gibberella Fujikuroi ◽  
Interleukin 4

ABSTRACT Fumonisin B1 (FB1), the principal secondary metabolite produced by the fungus Fusarium verticillioides (Gibberella fujikuroi mating population A), is a potent toxin that can be found in fungus-contaminated corn and corn-based food products. We have investigated the immunobiological effects of subchronic dietary exposure to FB1 in male Wistar rats. Animals were fed with diets containing 0 (control) or 100 ppm of FB1 for 12 weeks. The total FB1 intake on day 90 was 810 mg/kg of body weight. Food consumption, body weight, and body weight gain on day 90 were reduced in animals exposed to FB1. Histopathologic changes consisted of histiocytic perivascular infiltrate and an increased number of Kupffer cells in the liver, necrosis and apoptosis of tubular epithelial cells in the kidney, and increased mitotic figures and lymphocytic infiltrate in the small intestine. Serum enzyme alkaline phosphatase was significantly elevated in rats fed FB1, while triglyceride levels decreased compared to controls. Treatment with FB1 in vivo or in vitro did not have a significant effect on mitogen-induced proliferation of spleen mononuclear cells. However, increased levels of interleukin-4 (IL-4) and decreased levels of IL-10 were released by these cells in culture compared to controls. FB1 in vivo or in vitro decreased the hydrogen peroxide (H2O2) released by peritoneal macrophages, while no changes in levels of superoxide anion produced by total peritoneal cells were detected. The results from the present work demonstrate that subchronic FB1 intake could affect the small intestine and alter the interleukin profile and some main functions of macrophages in antitumor activity.

scholarly journals Efficacy of potential biocontrol agents for control of Fusarium verticillioides and fumonisin B1 under different environmental conditions

2016 ◽  
Vol 9 (2) ◽  
pp. 205-213 ◽  
Author(s):  
N.I.P. Samsudin ◽  
N. Magan
Keyword(s):  
Water Activity ◽  
Growth Rates ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Biocontrol Agents ◽  
Dual Culture ◽  
Clonostachys Rosea ◽  
Inhibition Studies ◽  
Spore Cell

A mycotoxigenic strain of Fusarium verticillioides previously isolated from Malaysian maize kernels and identified morphologically and molecularly was used in the present work. The objectives were (1) to screen the competitiveness of three potential antagonists isolated from Malaysian maize and three other known candidates for control of growth of F. verticillioides in vitro based on interaction scores, growth rates and hyphal area of F. verticillioides, and (2) to examine the best candidates using different spore/cell ratios on milled maize agar at different water activity conditions on relative control of fumonisin B1 (FB1). Three fungi and three bacteria (BCAs 1-6) were examined for antagonistic effects against F. verticillioides in dual-culture assays. These showed that all fungal candidates intermingled with F. verticillioides while all bacterial candidates inhibited F. verticillioides on contact or at a distance, which in turn decreased the growth rates and hyphal area of F. verticillioides significantly. Although BCA1 (Clonostachys rosea 016) did not inhibit growth or hyphal area of F. verticillioides, it was included in FB1 inhibition studies with other bacterial candidates (BCA4, Streptomyces sp. AS1; BCA5, Gram-negative rod; BCA6, Enterobacter hormaechei) because of its established mycoparasitism. The FB1 inhibition studies were conducted on milled maize agar with different spore/cell ratios of pathogen:antagonist mixtures at 0.95/0.98 water activity (aw) and 25 °C for 14 days. FB1 biosynthesis for all treatments was significantly higher at 0.95 than 0.98 aw. Of the four antagonists tested, the best was BCA1 which inhibited FB1 biosynthesis by 73 and 100% at 0.95 and 0.98 aw, respectively. BCA5 was the next best, resulting in 38 and 78% FB1 inhibition at 0.95 and 0.98 aw, respectively. The pathogen:antagonist ratios for BCA1 and BCA5 showed best results at 50:50 and 25:75. These results were discussed in context of using biocontrol agents to minimise fumonisins in maize.

scholarly journals Determination of the amount of bioaccessible fumonisin B1 in different matrices after in vitro digestion

2015 ◽  
Vol 8 (3) ◽  
pp. 261-267 ◽  
Author(s):  
J. Szabó-Fodor ◽  
C. Dall'Asta ◽  
C. Falavigna ◽  
M. Kachlek ◽  
Á. Szécsi ◽  
...  
Keyword(s):  
Analytical Methods ◽  
Fusarium Verticillioides ◽  
Animal Experiments ◽  
Extraction Procedure ◽  
Fumonisin B1 ◽  
In Vitro Digestion ◽  
The Matrix ◽  
Pre Treatment

Conventional analytical methods used for the analysis of fumonisin content in animal feeds fail to take into account the fumonisin content bound to the matrix, which is otherwise bioaccessible and can be absorbed from the gastrointestinal tract. Moreover, underestimation of fumonisin content using routine analytical methods can affect animal experiments using cereals contaminated by fungi. In the present study, hidden fumonisin B1 was analysed in two cereal substrates (maize and wheat) which were inoculated with Fusarium verticillioides (MRC 826). The study compared a routine extraction procedure with an in vitro digestion sample pre-treatment. We found that all samples showed a higher content of fumonisin B1 after digestion, compared to the free fumonisin obtained only by extraction. The percentage of the hidden form was 38.6% (±18.5) in maize and 28.3% (±17.8) in wheat, expressed as the proportion of total fumonisin B1. These results indicate that the toxin exposure of the animals determined by the routine fumonisin analysis was underestimated, generally by 40%, as bioaccessibility was not taken into consideration. This is crucial in interpretation (and maybe in re-evaluation) of the results obtained from (other) animal experiments.

scholarly journals Fumonisins production potential of Fusarium verticillioides isolated from Serbian maize and wheat kernels

2017 ◽  
pp. 71-78
Author(s):  
Sasa Krstovic ◽  
Sandra Jaksic ◽  
Aleksandra Bocarov-Stancic ◽  
Slavica Stankovic ◽  
Snezana Jankovic ◽  
...  
Keyword(s):  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Toxin Production ◽  
Hplc Method ◽  
Production Potential ◽  
Sample Extraction ◽  
Wheat Kernels ◽  
Yeast Extract Sucrose

The production of fumonisins by potentially toxigenic Fusarium verticillioides isolates originating from Serbian maize and wheat kernels was tested in vitro. A total of six F. verticillioides isolates were incubated on yeast extract sucrose medium (YESA) for 4 weeks at 25 ?C in the dark. Their toxin production potential was tested by applying a modified HPLC method for determination of fumonisins in cereals, since the TLC method gave no results. Analyses were performed on a HPLC-FLD system after sample extraction from YESA and extract cleanup on a SPE column. Although the isolates were tested for fumonisin B1, B2 and B3, only fumonisin B1 was detected. The results showed that all tested isolates had toxigenic potential for fumonisin B1 production. The average fumonisin B1 production of the isolates ranged from 7 to 289 ?g/kg, thus indicating a highly variable toxigenic potential among the isolates. Isolate 1282 expressed the highest toxigenic potential for fumonisin B1 production (289 ?g/kg), while isolate 2533/A showed a questionable potential for fumonisin production (7 ?g/kg).

Sign in / Sign up

Export Citation Format

Share Document