Application of Immobilized Lipase Enzyme for the Production of Biodiesel from Waste Cooking Oil

2013 ◽  
Vol 6 (7) ◽  
pp. 322-330 ◽  
Author(s):  
Srinidhi Desikan ◽  
Rekha Kannan ◽  
Krishnan Narayanan ◽  
D.J. Mukesh Kumar ◽  
P.T. Kalaichelv
Keyword(s):  
Immobilized Lipase ◽  
Waste Cooking Oil ◽  
Cooking Oil ◽  
Lipase Enzyme

“Statistical Optimization of Bio Diesel Production from Waste Cooking Oil Using Pva -Alginate Immobilized Lipase Enzyme as Bio Catalyst”

2020 ◽  
Author(s):  
Veeranna S. Hombalimath ◽  
Shameen Sultana M. Sultana ◽  
Sharanappa A. A. A ◽  
Anil R. Shet R. Shet ◽  
Laxmikant. R. patil R. Patil ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Waste Cooking Oil ◽  
Statistical Optimization ◽  
Cooking Oil ◽  
Lipase Enzyme ◽  
Diesel Production

Biodiesel production from waste cooking oil using a novel biocatalyst of lipase enzyme immobilized magnetic nanocomposite

Fuel ◽  
2022 ◽  
Vol 313 ◽  
pp. 123057
Author(s):  
Ehsan Parandi ◽  
Maryam Safaripour ◽  
Magda H. Abdellattif ◽  
Majid Saidi ◽  
Alireza Bozorgian ◽  
...  
Keyword(s):  
Waste Cooking Oil ◽  
Magnetic Nanocomposite ◽  
Biodiesel Production ◽  
Cooking Oil ◽  
Lipase Enzyme

scholarly journals Surfactant Imprinting Hyperactivated Immobilized Lipase as Efficient Biocatalyst for Biodiesel Production from Waste Cooking Oil

Catalysts ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 914 ◽  
Author(s):  
Yang ◽  
Zhang
Keyword(s):  
Burkholderia Cepacia ◽  
Energy Demand ◽  
Immobilized Lipase ◽  
Waste Cooking Oil ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Enzymatic Production ◽  
Burkholderia Cepacia Lipase ◽  
Cooking Oil ◽  
Highly Active

Enzymatic production of biodiesel from waste cooking oil (WCO) could contribute to resolving the problems of energy demand and environment pollutions.In the present work, Burkholderia cepacia lipase (BCL) was activated by surfactant imprinting, and subsequently immobilized in magnetic cross-linked enzyme aggregates (mCLEAs) with hydroxyapatite coated magnetic nanoparticles (HAP-coated MNPs). The maximum hyperactivation of BCL mCLEAs was observed in the pretreatment of BCL with 0.1 mM Triton X-100. The optimized Triton-activated BCL mCLEAs was used as a highly active and robust biocatalyst for biodiesel production from WCO, exhibiting significant increase in biodiesel yield and tolerance to methanol. The results indicated that surfactant imprinting integrating mCLEAs could fix BCL in their active (open) form, experiencing a boost in activity and allowing biodiesel production performed in solvent without further addition of water. A maximal biodiesel yield of 98% was achieved under optimized conditions with molar ratio of methanol-to-WCO 7:1 in one-time addition in hexane at 40 °C. Therefore, the present study displays a versatile method for lipase immobilization and shows great practical latency in renewable biodiesel production.

scholarly journals Production of Biodiesel from High Acid Value Waste Cooking Oil Using an Optimized Lipase Enzyme/Acid-Catalyzed Hybrid Process

2009 ◽  
Vol 6 (s1) ◽  
pp. S485-S495 ◽  
Author(s):  
N. Saifuddin ◽  
A. Z. Raziah ◽  
H. Nor Farah
Keyword(s):  
Acid Value ◽  
Reaction Rates ◽  
Waste Cooking Oil ◽  
Biodiesel Production ◽  
Hybrid Process ◽  
Initial Reaction ◽  
Cooking Oil ◽  
High Acid ◽  
Lipase Enzyme ◽  
Acid Catalyzed

The present study is aimed at developing an enzymatic/acid-catalyzed hybrid process for biodiesel production using waste cooking oil with high acid value (poor quality) as feedstock. Tuned enzyme was prepared using a rapid drying technique of microwave dehydration (time required around 15 minutes). Further enhancement was achieved by three phase partitioning (TPP) method. The results on the lipase enzyme which was subjected to pH tuning and TPP, indicated remarkable increase in the initial rate of transesterification by 3.8 times. Microwave irradiation was found to increase the initial reaction rates by further 1.6 times, hence giving a combined increase in activity of about 5.4 times. The optimized enzyme was used for hydrolysis and 88% of the oil taken initially was hydrolyzed by the lipase. The hydrolysate was further used in acid-catalyzed esterification for biodiesel production. By using a feedstock to methanol molar ratio of 1:15 and a sulphuric acid concentration of 2.5%, a biodiesel conversion of 88% was obtained at 50 °C for an hour reaction time. This hybrid process may open a way for biodiesel production using unrefined and used oil with high acid value as feedstock.

scholarly journals Enzymatic Production of Bio-Diesel from Waste Cooking Oil Using Lipase

2008 ◽  
Vol 2 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Sulaiman Al-Zuhair
Keyword(s):  
Immobilized Lipase ◽  
Sol Gel ◽  
Waste Cooking Oil ◽  
Molar Ratio ◽  
Free Form ◽  
Kinetics Parameters ◽  
Enzymatic Production ◽  
Cooking Oil ◽  
Free Lipase

The applications of lipase immobilized on ceramic beads and entrapped in sol-gel matrix, in the production of bio-diesel from waste cooking oil, are compared to that of free lipase. Experimental determination of the effect of molar equivalent of methanol, to moles of ester bond in the triglyceride, on the rate of the enzymatic trans-esterification was experimentally determined. It was found that for the same weight of lipase used, the production of bio-diesel was much higher using lipase immobilized on ceramic beads in comparison to that using lipase entrapped in sol-gel and in free form. Substrates inhibition effect was observed in all cases, which agrees with previous results found in literature. The optimum methanol:oil molar ratio was found to be 0.87 for immobilized lipase from yeast source, C. antartica and 1.00 for free lipase from the same yeast source and immobilized lipase from bacterial source, P. cepacia. On the other hand, it was shown that biodieasel can be produced in considerable amounts, with yield reaching 40%, in absence of organic solvent using immobilized lipase, from P. cepacia, on ceramic beads. The results of this study can be used to determine the kinetics parameters of mathematical models which describe the system.

scholarly journals Synthesis of biodiesel from waste cooking oil using immobilized lipase in fixed bed reactor

2009 ◽  
Vol 50 (3) ◽  
pp. 668-673 ◽  
Author(s):  
Yingming Chen ◽  
Bo Xiao ◽  
Jie Chang ◽  
Yan Fu ◽  
Pengmei Lv ◽  
...  
Keyword(s):  
Immobilized Lipase ◽  
Fixed Bed ◽  
Waste Cooking Oil ◽  
Fixed Bed Reactor ◽  
Cooking Oil
Sign in / Sign up

Export Citation Format

Share Document