scholarly journals Effects of Sodium Butyrate on the Intestinal Morphology and DNA-Binding Activity of Intestinal Nuclear Factor-κB in Weanling Pigs

2012 ◽  
Vol 11 (6) ◽  
pp. 814-821 ◽  
Author(s):  
Jian-Jun Lu . ◽  
Zheng-Shun Wen ◽  
Xiao-Ting Zou .
Keyword(s):  
Dna Binding ◽  
Sodium Butyrate ◽  
Nuclear Factor Κb ◽  
Binding Activity ◽  
Intestinal Morphology ◽  
Nuclear Factor ◽  
Weanling Pigs ◽  
Dna Binding Activity

UV stimulation induces nuclear factor κB (NF-κB) DNA-binding activity but not transcriptional activation

2001 ◽  
Vol 29 (6) ◽  
pp. 688-691 ◽  
Author(s):  
K. J. Campbell ◽  
N. R. Chapman ◽  
N. D. Perkins
Keyword(s):  
Dna Binding ◽  
Transcriptional Activation ◽  
Cellular Response ◽  
Uv Light ◽  
Binding Protein ◽  
Nuclear Factor Κb ◽  
Camp Response Element Binding ◽  
Binding Activity ◽  
Nuclear Factor ◽  
Dna Binding Activity

The cellular response to DNA-damaging agents is partly mediated by DNA-binding transcription factors such as p53 and nuclear factor κB (NF-κB). Typically NF-κB activation is associated with resistance to apoptosis. Following stimulation with UV light however, NF-κB activation has been shown to be required for programmed cell death. To study this effect further and to determine the relationship between NF-κB and p53 function, we have examined the effect of UV light on U2OS cells. UV stimulation resulted in the activation of NF-κB DNA-binding and the induction of p53. Surprisingly, and in contrast with tumour necrosis factor α stimulation, this UV-induced NF-κB was transcriptionally inert. These observations suggest a model in which the NF-κB switch from an anti-apoptotic to a pro-apoptotic role within the cell results from modulation of its ability to stimulate gene expression, possibly as a result of the ability of p53 to sequester transcriptional co-activator proteins such as p300/CREB (cAMP-response-element-binding protein)-binding protein.

scholarly journals UV-A-induced decrease in nuclear factor-κB activity in human keratinocytes

1999 ◽  
Vol 338 (3) ◽  
pp. 607-613 ◽  
Author(s):  
Mojgan DJAVAHERI-MERGNY ◽  
Marie-Pierre GRAS ◽  
Jean-Louis MERGNY ◽  
Louis DUBERTRET
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Uv Radiation ◽  
Human Keratinocytes ◽  
Nuclear Factor Κb ◽  
Binding Activity ◽  
Luciferase Reporter ◽  
Nuclear Factor ◽  
Protein Subunits ◽  
Dna Binding Activity

Previous reports have demonstrated an increase in nuclear factor-κB (NF-κB) activity in response to UV radiation. These studies have essentially focused on the DNA-damaging fraction of solar UV radiation (UV-B and UV-C). In contrast, the effects of UV-A radiation (320–400 nm) on NF-κB are not well known. In this study, we present evidence that UV-A radiation induces a marked decrease in NF-κB DNA-binding activity in NCTC 2544 human keratinocytes. In addition, NCTC 2544 keratinocytes pretreated with UV-A fail to respond to NF-κB inducers. Moreover, UV-A radiation induces a decrease in NF-κB-driven luciferase reporter gene expression in NCTC 2544 keratinocytes. The expression of the gene encoding IκBα (IκB is the NF-κB inhibitor), which is closely associated with NF-κB activity, is also reduced (3-fold) upon UV-A treatment. Our results indicate that the UV-A-induced decrease in NF-κB DNA-binding activity is associated with a decrease in the levels of the p50 and p65 protein subunits. This is the first evidence that an oxidative stress, such as UV-A radiation, may induce a specific decrease in NF-κB activity in mammalian cells, probably through degradation of NF-κB protein subunits. These findings suggest that UV-A could modulate the NF-κB-dependent gene expression.

Decreased nuclear factor-κB DNA binding activity following permanent focal cerebral ischaemia in the rat

2000 ◽  
Vol 288 (1) ◽  
pp. 45-48 ◽  
Author(s):  
E.A Irving ◽  
S.J Hadingham ◽  
J Roberts ◽  
M Gibbons ◽  
M Chabot-Fletcher ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cerebral Ischaemia ◽  
Nuclear Factor Κb ◽  
Binding Activity ◽  
Nuclear Factor ◽  
Focal Cerebral Ischaemia ◽  
Dna Binding Activity

Acute stress enhances contact dermatitis by promoting nuclear factor-κB DNA-binding activity and interleukin-18 expression in mice

2010 ◽  
Vol 37 (6) ◽  
pp. 512-521 ◽  
Author(s):  
Jing ZHANG ◽  
Lingjiang LI ◽  
Qianjin LU ◽  
Rong XIAO ◽  
Haiquan WEN ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Dna Binding ◽  
Acute Stress ◽  
Nuclear Factor Κb ◽  
Binding Activity ◽  
Nuclear Factor ◽  
Interleukin 18 ◽  
Dna Binding Activity

Vitamin E deficiency induces liver nuclear factor-κB DNA-binding activity and changes in related genes

2005 ◽  
Vol 39 (10) ◽  
pp. 1127-1138 ◽  
Author(s):  
María Morante ◽  
Juan Sandoval ◽  
Mari-Carmen Gómez-Cabrera ◽  
José L. Rodríguez ◽  
Federico V. Pallardó ◽  
...  
Keyword(s):  
Vitamin E ◽  
Dna Binding ◽  
Nuclear Factor Κb ◽  
Binding Activity ◽  
Nuclear Factor ◽  
Vitamin E Deficiency ◽  
Dna Binding Activity

Nuclear factor-κB in rat brain: Enhanced DNA-binding activity following convulsant-induced seizures

1994 ◽  
Vol 170 (1) ◽  
pp. 145-148 ◽  
Author(s):  
Adapa V. Prasad ◽  
Webster H. Pilcher ◽  
Shirley A. Joseph
Keyword(s):  
Dna Binding ◽  
Rat Brain ◽  
Nuclear Factor Κb ◽  
Binding Activity ◽  
Nuclear Factor ◽  
Dna Binding Activity
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