Evaluation of Antimicrobial Potential of a Galactose-Specific Lectin in the Skin Mucus of African Catfish (Clarias gariepinus, Burchell, 1822) against some Aquatic Microorganisms

Antimicrobial peptides (AMPs) constitute a broad range of bioactive compounds in diverse organisms, including fish. They are effector molecules for the innate immune response, against pathogens, tissue damage and infections. Still, AMPs from African Catfish, Clarias gariepinus, skin mucus are largely unexplored despite their possible therapeutic role in combating antimicrobial resistance. In this study, African Catfish Antimicrobial peptides (ACAPs) were identified from the skin mucus of African Catfish, C. gariepinus. Native peptides were extracted from fish mucus scrapings in 10% acetic acid (v/v) and ultra-filtered using 5 kDa molecular weight cut-off membrane. The extract was purified using C18 Solid-Phase Extraction. The antibacterial activity was determined using the Agar Well Diffusion method and broth-dilution method utilizing Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Thereafter, Sephadex G-25 gel filtration was further utilized in bio-guided isolation of the most active fractions prior to peptide identification using Orbitrap Fusion Lumos Tribrid Mass Spectrometry. The skin mucus extracted from African Catfish from all the three major lakes of Uganda exhibited antimicrobial activity on E. coli and S. aureus. Lake Albert’s C. gariepinus demonstrated the best activity with the lowest MIC of 2.84 and 0.71 μg/ml on S. aureus and E. coli, respectively. Sephadex G-25 peak I mass spectrometry analysis (Data are available via ProteomeXchange with identifier PXD029193) alongside in silico analysis revealed seven short peptides (11–16 amino acid residues) of high antimicrobial scores (0.561–0.905 units). In addition, these peptides had a low molecular weight (1005.57–1622.05 Da) and had percentage hydrophobicity above 54%. Up to four of these AMPs demonstrated α-helix structure conformation, rendering them amphipathic. The findings of this study indicate that novel AMPs can be sourced from the skin mucus of C. gariepinus. Such AMPs are potential alternatives to the traditional antibiotics and can be of great application to food and pharmaceutical industries; however, further studies are still needed to establish their drug-likeness and safety profiles.

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PERBEDAAN DAYA TAHAN NON SPESIFIK LIMA SPESIES IKAN AIR TAWAR TERHADAP INFEKSI Aeromonashydrophila

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.9031 ◽

2003 ◽

Vol 5 (2) ◽

pp. 1

Author(s):

H. Syakuri ◽

Triyanto Triyanto ◽

K. H. Nitimulyo

Keyword(s):

Antibacterial Activity ◽

Common Carp ◽

Antibody Titer ◽

Fish Species ◽

Nile Tilapia ◽

Clarias Gariepinus ◽

Causative Factor ◽

African Catfish ◽

Skin Mucus ◽

Time To Death

Fish naturally has a non-specific defense against several pathogens. The non-specific defense is anatomical and physiological function that varies according to genetical and environmental factor. The differences of non-specific defense against Aeromonas hydrophila infection were studied in blackfinpacu (Colossoma macropomum), gourami (Osphronemus goramy), common crap (Cyprinus carpio), African catfish (Clarias gariepinus), and red nile tilapia (Orochromis sp.). This study also examined the differences on several parameters of non-specific defense, including differentiation anc number of leucocytes, serum total, titer of antibody, and antibacterial activity of skin mucus.The fishes were intramuscularly infected at the media lethal doses for common carp (7,4 x 108cfu/fish). Pathological changes, survival rate, and mean time to death were observed every day. The observation of non-specific defense parameters were carried out prior and at seven day after infection.The results showed thahblackfinpacu was the most resistant species against A. hydrophila infection. African catfish and red nile tilapia were most resistant than gourami and common carp. The antibody titer and percentage of thrombocytes were the causative factor for the difference of the resistence against A. hydrophila. The five fish species also showed differences on leucocytes number, monocytes and eosinophiles percentage, and serum total, but they did not showed the differences of neutrophile percentage. The skin mucus of all fish species did not exhibited antibacterial activity against A. hydrophila. This study also found increase in antibody titer and leucocytes number after A. hydrophila infection.

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Charaterization of galactose-specific lectin from the skin mucus of African catfish Clarias gariepinus aBurchell, 1822

Scientific Research and Essays ◽

10.5897/sre2014.5995 ◽

2014 ◽

Vol 9 (20) ◽

pp. 869-879 ◽

Cited By ~ 1

Author(s):

Oludele Olayemi ODEKANYIN ◽

Adenike KUKU

Keyword(s):

Clarias Gariepinus ◽

African Catfish ◽

Skin Mucus

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EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat ◽

10.32504/sm.v14i1.106 ◽

2019 ◽

Vol 14 (1) ◽

pp. 26

Author(s):

Dyah Anggraeni ◽

Nurlela Nurlela

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Research Result ◽

Clarias Gariepinus ◽

Storage Period ◽

The Body ◽

Garlic Extract ◽

African Catfish ◽

Natural Preservatives ◽

Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L). Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus). Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter. Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

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PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN

Jurnal Natural ◽

10.30862/jn.v9i1.778 ◽

2010 ◽

Vol 9 (1) ◽

Author(s):

Lutfi Lutfi

Keyword(s):

Liquid Nitrogen ◽

Clarias Gariepinus ◽

African Catfish ◽

Nitrogen Vapor ◽

Dmso Concentration ◽

Freezing Temperatures ◽

Vapor Liquid

Effect of four types of diluentss and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. The steps in preparation the 16 treatments combination of the diluents are preparation of diluents, mixing diluents with DMSO, packing of semen into 0.3-ml straw, equilibration of semen at 4 °C for 30 minutes, freezing of semen in nitrogen vapor liquid at a height of 6.5 cm for 10 minutes, and subsequently storage of semen in liquid nitrogen (-196 0C) for further analysis of post-thawing motility (PTM). The result showed that the highest level of motility of spermatozoa was in treatment P1D15 (45.7 ± 4.3%) and the lowest was in treatment P2D20 (14.5 ± 13.2%). The best diluent in this observation was diluents containing NaCl, KCl, CaCl2 and NaHCO3. The best concentration was DMSO 15%. While the best interaction between the concentration of DMSO diluents is P1D15 treatments containing NaCl, KCl, CaCl 2 and NaHCO3 with a combination of 15% DMSO concentration. The conclusion of the research is that diluents containing NaCl, KCl, CaCl2 and NaHCO3 with a combination of 15% DMSO concentration can be used in cryopreservation of African catfish semen.

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