scholarly journals The INRA-CIRAD citrus germplasm collection of San Giuliano, Corsica

2018 ◽  
Author(s):  
François Luro ◽  
Emmanuel Bloquel ◽  
Bruno Tomu ◽  
Gilles Costantino ◽  
Isabelle Tur ◽  
...  
Keyword(s):  
Germplasm Collection ◽  
Citrus Germplasm

PROSPECTS FOR THE USE OF REMOTE CROSS IN THE CITRUS CROPS SELECTION

1970 ◽  
pp. 46-49 ◽  
Author(s):  
R. V. Kulyan
Keyword(s):  
Complex Traits ◽  
Germplasm Collection ◽  
Early Maturity ◽  
Russian Research Institute ◽  
Citrus Germplasm ◽  
The Status ◽  
New Varieties ◽  
Primary Test ◽  
Growing Conditions ◽  
Positive Traits

The Russian Research Institute of Floriculture and Subtropical Crops has the citrus germplasm collection, in total over 150 genotypes of various origins including 30 wild and semi-wild relatives. As a result of controlled hybridization in 17 crossings combinations of with the participation of relatives of citrus plants, new 769 hybrid offspring were obtained, which combine the traits of both the maternal and paternal genotypes. Analyzing the populations, promising combinations were highlighted: C. reticulata × Fortunella margarita (47.1%); C. x natsudaidai × 3252 (42.1%) and C. reticulata × C. reticulata ‘Cleopatra’ (40.9%) to create the gene pool of distant hybrids. From the mentioned combinations of crossings the greatest percent of seedlings which phenotypes tend to cultivated varieties was received. This hybrid material is a valuable source for isolating forms that are resistant to extreme environmental factors. According to phenotypic characteristics, hybrids were divided into three categories: I – Cultural, II – Semi-wild and III – Wild. Of the first category, the largest number 87 prospective forms were selected, and can be of interest for further breeding. As a result of the study of interspecific hybrid seedlings, 137 promising forms have been identified, which are carriers the complex traits such as dwarfism, thornless, early maturity and increased winter hardiness. From this set 17 genotypes were selected, which received the status of an elite forms, which successfully pass the primary test, and will be also useful in further breeding work for creating sources with a complex of positive traits and on breeding new varieties of citrus crops resistant to growing conditions.

Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers

2011 ◽  
Vol 129 (4) ◽  
pp. 798-803 ◽  
Author(s):  
Manosh Kumar Biswas ◽  
Lijun Chai ◽  
Mohamed Hamdy Amar ◽  
Xianlong Zhang ◽  
Xiu-xin Deng
Keyword(s):  
Genetic Diversity ◽  
Comparative Analysis ◽  
Ssr Markers ◽  
Germplasm Collection ◽  
Citrus Germplasm

Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection

2011 ◽  
Vol 128 (3) ◽  
pp. 220-227 ◽  
Author(s):  
Mohamed Hamdy Amar ◽  
Manosh Kumar Biswas ◽  
Zongwen Zhang ◽  
Wen-Wu Guo
Keyword(s):  
Genetic Diversity ◽  
Germplasm Collection ◽  
Snp Markers ◽  
Citrus Germplasm

scholarly journals Resistance to Citrus Canker Caused by Xanthomonas axonopodis pv. citri in an Accession of Citrus sp. Similar to Grapefruit

Plant Disease ◽  
2008 ◽  
Vol 92 (4) ◽  
pp. 652-652 ◽  
Author(s):  
A. M. Gochez ◽  
B. I. Canteros
Keyword(s):  
High Resistance ◽  
Quantitative Resistance ◽  
Germplasm Collection ◽  
Citrus Canker ◽  
Citrus Paradisi ◽  
Xanthomonas Axonopodis ◽  
Citrus Germplasm ◽  
Entre Rios ◽  
Short Time

Citrus canker is caused by Xanthomonas axonopodis pv. citri. All grapefruit (Citrus paradisi Macf.) cultivars are highly susceptible to the A-group strains of Xanthomonas axonopodis pv. citri. A type of grapefruit (Citrus sp.) called Dalan Dan (DD) has shown high resistance in the field in Misiones, Argentina where it is widely planted. The DD is Accession No. NC678 in the Campinas Citrus Germplasm Collection (Centro APTA Citrus Sylvio Moreira, Cordeiropolis, SP, Brazil) and Accession No. CCC-618 Dalan Dan in the Argentina Citrus Germplasm Collection at INTA (Concordia, Entre Ríos). Molecular studies completed in Brazil have shown high similarities among grapefruit varieties and DD (1). We report here the characterization of quantitative resistance to citrus canker in DD. Quantitative resistance in citrus is expressed as lower number of lesions per leaf area (3). Lesions per square centimeter were calculated after 3 to 4 weeks of two sets of inoculation of three inoculum levels (5 × 102, 2.5 × 103, and 5 × 103 cells per ml) in leaves of developing leaf flushes. The period of greatest susceptibility in DD was 15 to 23 days for the three inoculum levels; 1.35 ± 0.34 to 0.01 ± 0.01, 4.98 ± 0.91 to 0.91 ± 0.48, and 9.85 ± 2.17 to 1.91 ± 1.37 lesions per cm2, respectively. By contrast, the period of greatest susceptibility in grapefruit cv. Duncan was 15 to 29 days for the same three inoculum levels; 2.78 ± 0.46 to 1.22 ± 0.39, 17.29 ± 2.79 to 5.55 ± 0.93, and 22.87 ± 3.27 to 11.95 ± 3.55 lesions per cm2, respectively. The results shown here suggest that the DD citrus accession is susceptible to citrus canker for a very short time during the development of leaf flushes, which results in very high resistance in the field. This citrus germplasm material could be valuable as a source of resistance to improve other varieties or as a replacement of grapefruit due to the similar fruit and juice qualities. References: (1) M. J. Corazza-Nunes et al. Euphytica 126:169, 2002. (2) M. J. Corazza-Nunes et al. Summa Phytopathol. 32:322, 2006. (3) R. E. Stall et al. Phytopathology 72:1097, 1982.

scholarly journals RAPD primer screening as a preliminary study to analyze the genetic diversity of Citrus spp. in South Sulawesi, Indonesia

2021 ◽  
Vol 886 (1) ◽  
pp. 012017
Author(s):  
M Tuwo ◽  
T Kuswinanti ◽  
A Nasruddin ◽  
E Tambaru
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Germplasm Collection ◽  
Breeding Programs ◽  
Citrus Breeding ◽  
South Sulawesi ◽  
Citrus Germplasm ◽  
Rapd Primer ◽  
Preliminary Study ◽  
Polymorphic Bands

Abstract The typical citrus germplasm collection in South Sulawesi has not been thoroughly characterized, especially in several citrus development centers, which have begun to be promoted again after the decline in productivity due to CVPD infection. The study of citrus diversity is very important to support future citrus breeding programs. Random amplified polymorphic DNA (RAPD) has been widely used for the analysis of genetic diversity among species in populations. In this study, 23 RAPD primers were used on Citrus cultivated in Selayar and Pangkep Regencies, which are citrus development areas in South Sulawesi. A total of 19 primers (OPA-05, OPA-09, OPA-17, OPC-09, OPC-17, OPE-04, OPH-04, OPH-15, OPN-14, 0PN-16, OPR-08, OPR-20, OPW-06, OPW-09, OPX-07, OPX-11, OPX-17, UBC-18, and UBC-51) can form polymorphic bands in randomly selected DNA samples. Monomorphic bands were formed by OPA-12 and OPD-07 primer in 12 samples. The primers OPX-13 and OPX-16 produced unclear bands. These 19 primers can be used to amplify DNA and determine the genetic diversity of Citrus in further analysis.

scholarly journals Karakterisasi dan Pemanfaatan Plasma Nutfah Jeruk In Situ oleh Masyarakat Lokal Sumatera Utara

2016 ◽  
Vol 15 (2) ◽  
pp. 70
Author(s):  
Sortha Simatupang
Keyword(s):  
Vitamin C ◽  
Traditional Medicine ◽  
Local Community ◽  
Germplasm Collection ◽  
Processed Food ◽  
Salt Tolerant ◽  
Mangrove Area ◽  
Citrus Germplasm ◽  
Soluble Solid

<p>Characterization and utilization of in situ citrus germplasm of collection maintained by local community. This study was aimed at characterizing of in situ citrus germplasm collection and their use. This Study was conducted through interviewing citrus retailers as well as communinity leaders from January- December 2004. There were 33 citrus accescions found in this study. Local communinity utilized citrus in several different ways such as traditional medicine, ingredient of processed food as well as consumed as fresh fruit. Five accescions such as Laukawar, Keprok Sipirok, Boci, Maga, and Keling were sweet and juicy. In addition, Laukawar and Boci, both are seedless. There were four accescions, Andaliman, Purut, Sate, and Gajah having strong citrus scent. Lemon Tea, Nipis, seedless Nipis, Begu, Purut, Sunde, and Sate were used as ingredient of processed food. Gajah, Purut, Pagar, Malem, Kuku Harimau, Kersik, Kapas, Kayu, Puraga, and Kelele were used as traditional medicine. Keling Karo had highest vitamin C content (12 mg/100 mg). Laukawar had the highest total soluble solid. Bunian seemed to be salt tolerant one grown in mangrove area.</p><p> </p><p><strong>Abstrak</strong></p><p>Penelitian bertujuan untuk mendapatkan karakterisasi plasma nutfah jeruk in situ dan pemanfaatannya oleh masyarakat lokal Sumatera Utara. Metode yang digunakan adalah eksplorasi. Wawancara terbuka tanpa kuesioner dilakukan pada pedagang jeruk yang dikros cek dengan para sesepuh masyarakat lokal. Survei dilakukan pada bulan Januari sampai Desember 2004. Dari eksplorasi diperoleh 33 aksesi jeruk di Sumatera Utara yang terpelihara secara in situ. Masyarakat lokal memanfaatkannya sebagai obat tradisional, bahan campuran olahan pangan, dan sebagai buah segar. Lima aksesi mempunyai rasa manis dan berair, yaitu jeruk Laukawar, Keprok Sipirok, Boci, Maga, dan Keling. Laukawar dan Boci adalah jeruk tanpa biji untuk konsumsi segar. Jeruk yang mempunyai aroma sangat kuat adalah Andaliman (skor 9), jeruk Purut, jeruk Sate, dan jeruk Gajah (skor 8). Jeruk olahan untuk campuran pangan, yaitu Lemon Tea, Nipis, Nipis Tanpa Biji, Begu, Purut, Sunde, dan Sate. Jeruk untuk obat tradisional adalah Gajah, Purut, Pagar, Malem, Kuku Harimau, Kersik, Kapas, Kayu, Puraga, dan Kelele. Kandungan vitamin C tertinggi terdapat pada jeruk Keling Karo (12 mg/100 mg). Jeruk dengan padatan total terlarut tertinggi terdapat pada jeruk Laukawar (15oBrix). Terdapat satu aksesi jeruk yang toleran salinitas, yaitu jeruk Bunian, yang tumbuh di daerah mangrove.</p>

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