scholarly journals Karakterisasi dan Pemanfaatan Plasma Nutfah Jeruk In Situ oleh Masyarakat Lokal Sumatera Utara

2016 ◽  
Vol 15 (2) ◽  
pp. 70
Author(s):  
Sortha Simatupang

<p>Characterization and utilization of in situ citrus germplasm of collection maintained by local community. This study was aimed at characterizing of in situ citrus germplasm collection and their use. This Study was conducted through interviewing citrus retailers as well as communinity leaders from January- December 2004. There were 33 citrus accescions found in this study. Local communinity utilized citrus in several different ways such as traditional medicine, ingredient of processed food as well as consumed as fresh fruit. Five accescions such as Laukawar, Keprok Sipirok, Boci, Maga, and Keling were sweet and juicy. In addition, Laukawar and Boci, both are seedless. There were four accescions, Andaliman, Purut, Sate, and Gajah having strong citrus scent. Lemon Tea, Nipis, seedless Nipis, Begu, Purut, Sunde, and Sate were used as ingredient of processed food. Gajah, Purut, Pagar, Malem, Kuku Harimau, Kersik, Kapas, Kayu, Puraga, and Kelele were used as traditional medicine. Keling Karo had highest vitamin C content (12 mg/100 mg). Laukawar had the highest total soluble solid. Bunian seemed to be salt tolerant one grown in mangrove area.</p><p> </p><p><strong>Abstrak</strong></p><p>Penelitian bertujuan untuk mendapatkan karakterisasi plasma nutfah jeruk in situ dan pemanfaatannya oleh masyarakat lokal Sumatera Utara. Metode yang digunakan adalah eksplorasi. Wawancara terbuka tanpa kuesioner dilakukan pada pedagang jeruk yang dikros cek dengan para sesepuh masyarakat lokal. Survei dilakukan pada bulan Januari sampai Desember 2004. Dari eksplorasi diperoleh 33 aksesi jeruk di Sumatera Utara yang terpelihara secara in situ. Masyarakat lokal memanfaatkannya sebagai obat tradisional, bahan campuran olahan pangan, dan sebagai buah segar. Lima aksesi mempunyai rasa manis dan berair, yaitu jeruk Laukawar, Keprok Sipirok, Boci, Maga, dan Keling. Laukawar dan Boci adalah jeruk tanpa biji untuk konsumsi segar. Jeruk yang mempunyai aroma sangat kuat adalah Andaliman (skor 9), jeruk Purut, jeruk Sate, dan jeruk Gajah (skor 8). Jeruk olahan untuk campuran pangan, yaitu Lemon Tea, Nipis, Nipis Tanpa Biji, Begu, Purut, Sunde, dan Sate. Jeruk untuk obat tradisional adalah Gajah, Purut, Pagar, Malem, Kuku Harimau, Kersik, Kapas, Kayu, Puraga, dan Kelele. Kandungan vitamin C tertinggi terdapat pada jeruk Keling Karo (12 mg/100 mg). Jeruk dengan padatan total terlarut tertinggi terdapat pada jeruk Laukawar (15oBrix). Terdapat satu aksesi jeruk yang toleran salinitas, yaitu jeruk Bunian, yang tumbuh di daerah mangrove.</p>

2019 ◽  
pp. 77-83

L-Galactosa deshidrogenasa y L-Gulono-1,4-lactona deshidrogenasa influyen en la biosíntesis de vitamina C en Myrciaria dubia (Kunth) McVaugh “camu-camu” L-Galactose dehydrogenase and L-Gulone-1,4-lactone dehydrogenase influence in biosynthesis of vitamin C in Myrciaria dubia (Kunth) McVaugh “camu-camu” Juan C. Castro, Angel Araujo, Freddy Gutiérrez, Sixto A. Imán Laboratorio de Biotecnología. Centro de Investigaciones de Recursos Naturales de la Amazonía (CIRNA),bUniversidad Nacional de la Amazonia Peruana (UNAP). Pasaje Los Paujiles S/N. AAHH Nuevo San Lorenzo. Distrito de San Juan Bautista-Iquitos Instituto Nacional de Innovación Agraria (INIA). San Roque. Iquitos-Perú. DOI: https://doi.org/10.33017/RevECIPeru2012.0024/ Resumen La vitamina C es un nutriente esencial para el hombre y una de sus fuentes naturales son los frutos de Myrciaria dubia. Pero esta planta muestra amplia variación en contenido de vitamina C. Para ayudar a esclarecer las bases bioquímicas de esta variación, nos planteamos como objetivos determinar si las enzimas L-Galactosa deshidrogenasa (L-GalDH) y L-Gulono-1,4-lactona deshidrogenasa (L-GuLDH) están presentes en las hojas y frutos (pulpa y cáscara) del camu-camu y comparar la actividad catalítica de ambas enzimas en la pulpa de frutos cosechados de plantas que producen alto y bajo contenido de vitamina C. Las hojas y frutos fueron obtenidos de seis plantas (tres que producen bajo y tres que producen alto contenido de vitamina C) de la colección de germoplasma del INIA. La vitamina C fue extraída y cuantificada mediante HPLC. Posteriormente, de ambos grupos de plantas, se purificó parcialmente las enzimas y midió por triplicado su actividad catalítica mediante espectrofotometría. Tanto L-GalDH como L-GuLDH fueron detectadas en las hojas, pulpa y cáscara. Ambas enzimas mostraron diferencias en sus actividades catalíticas entre los tejidos analizados (p < 0,05). Al comparar la actividad de estas enzimas entre plantas que producen frutos con alto (2258±217 mg Vit. C/100g pulpa) y bajo (1570±46 mg Vit. C/100g pulpa) contenido de vitamina C se encontró diferencias significativas (p < 0,05) entre ambos grupos. Siendo mayor la actividad catalítica de L-GalDH y L-GuLDH en frutos con alto contenido de vitamina C y mostraron menor actividad catalítica en los frutos con bajo contenido de esta vitamina. Adicionalmente, ensayos cinéticos realizados con la L-GuLDH del camu-camu mostraron que esta enzima tiene una alta afinidad (Km = 2,37 M y Vmax = 9,23 mol.mg prot-1 .min-1) por su sustrato L-Gulono-1,4-lactona. En conclusión, las enzimas L-Galactosa deshidrogenasa y L-Gulono-1,4-lactono deshidrogenasa están presentes en las hojas y frutos del camu-camu, lo que nos indica que las dos vías biosintéticas de vitamina C propuestas para las plantas (vía de Smirnoff-Wheeler y vía de Wolucka), determinan la producción de vitamina C en M. dubia. Asimismo, las evidencias experimentales sugieren que la acumulación de vitamina C en la pulpa de los frutos del camu-camu depende por lo menos de dos procesos: biosíntesis in situ y transporte desde otros tejidos con capacidad biosintética. Además, se evidencia que la actividad catalítica de L-GalDH y L-GulDH influyen en el contenido de vitamina C de la pulpa, de tal modo que una mayor actividad catalítica de ambas enzimas está asociada con más contenido de vitamina C en este tejido. Palabras clave: L-galactosa deshidrogenasa, L-gulono-1,4-lactona deshidrogenasa, biosíntesis de vitamina C, Myrciaria dubia. Abstract Vitamin C is an essential nutrient for humans and one their natural sources are fruits of Myrciaria dubia. But this plant shows wide variation in vitamin C. To help clarify the biochemical basis of this variation, we plan aims to determine whether the enzymes L-Galactose dehydrogenase (L-GalDH) and L-Gulone-1,4-Lactone dehydrogenase (L-GuLDH) are presents in leaves and fruits (pulp and peel) of camu-camu and compare catalytic activity of both enzymes in pulp of fruits harvested from plants that produce high and low content of vitamin C. Leaves and fruits were obtained from six plants (three producing low and three that produce high content of vitamin C) in the germplasm collection of INIA. Vitamin C was extracted and quantified by HPLC. Subsequently, from both groups of plants, was partially purified enzymes and measured by triplicate their catalytic activity by means of spectrophotometry. Both L-GalDH and L-GuLDH were detected in leaves, pulp and peel. Both enzymes showed differences in their catalytic activities among the tissues analyzed (p < 0.05). By comparing the activity of these enzymes between plants that produces fruits with high (2258±217 mg Vit. C/100g pulpa) and low (1570±46 mg Vit. C/100g pulpa) content of vitamin C significant differences were found (p <0.05) between groups. Being higher the catalytic activity of L-GalDH and L-GuLDH in fruits with high content in vitamin C and showed lower catalytic activity in fruits with low content in this vitamin. In addition, kinetic assays performed with the L-GuLDH of camu-camu showed that this enzyme has a high affinity (Km = 2.37 M y Vmax = 9.23 mol.mg prot-1 .min-1) for its substrate L-Gulone-1,4-lactone. In conclusion, the enzymes L-galactose dehydrogenase and L-gulone-1,4-lactone dehydrogenase are present in leaves and fruits of camu-camu, which indicates that the two biosynthetic pathways of vitamin C proposals for plants (pathway of Smirnoff-Wheeler and pathway of Wolucka) determine production of vitamin C in M. dubia. Furthermore, experimental evidences suggest that the accumulation of vitamin C in the pulp of the fruits of camu-camu depends at least of two processes: in situ biosynthesis and transport from other tissues with biosynthetic capacity. Furthermore, it is evident that catalytic activity of L-GalDH and L-GulDH influence in vitamin C content of pulp, so that a higher catalytic activity of both enzymes is associated with more vitamin C content in this tissue. Keywords: L-galactose dehydrogenase, L-gulone-1,4-lactone dehydrogenase, biosynthesis of vitamin C, Myrciaria dubia.


Author(s):  
R. V. Kulyan

The Russian Research Institute of Floriculture and Subtropical Crops has the citrus germplasm collection, in total over 150 genotypes of various origins including 30 wild and semi-wild relatives. As a result of controlled hybridization in 17 crossings combinations of with the participation of relatives of citrus plants, new 769 hybrid offspring were obtained, which combine the traits of both the maternal and paternal genotypes. Analyzing the populations, promising combinations were highlighted: C. reticulata × Fortunella margarita (47.1%); C. x natsudaidai × 3252 (42.1%) and C. reticulata × C. reticulata ‘Cleopatra’ (40.9%) to create the gene pool of distant hybrids. From the mentioned combinations of crossings the greatest percent of seedlings which phenotypes tend to cultivated varieties was received. This hybrid material is a valuable source for isolating forms that are resistant to extreme environmental factors. According to phenotypic characteristics, hybrids were divided into three categories: I – Cultural, II – Semi-wild and III – Wild. Of the first category, the largest number 87 prospective forms were selected, and can be of interest for further breeding. As a result of the study of interspecific hybrid seedlings, 137 promising forms have been identified, which are carriers the complex traits such as dwarfism, thornless, early maturity and increased winter hardiness. From this set 17 genotypes were selected, which received the status of an elite forms, which successfully pass the primary test, and will be also useful in further breeding work for creating sources with a complex of positive traits and on breeding new varieties of citrus crops resistant to growing conditions.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 638
Author(s):  
Marcelo B. Medeiros ◽  
José F. M. Valls ◽  
Aluana G. Abreu ◽  
Gustavo Heiden ◽  
Suelma Ribeiro-Silva ◽  
...  

This study presents the status of ex situ and in situ conservation for the crop wild relatives of rice, potato, sweet potato, and finger millet in Brazil, and the subsequent germplasm collection expeditions. This research is part of a global initiative entitled “Adapting Agriculture to Climate Change: Collecting, Protecting, and Preparing Crop Wild Relatives” supported by the Global Crop Diversity Trust. Species of the primary, secondary, and tertiary gene pools with occurrences reported in Brazil were included: Oryza alta Swallen, O. grandiglumis (Döll) Prod., O. latifolia Desv., O. glumaepatula Steud., Eleusine tristachya (Lam.) Lam., E. indica (L.) Gaertn., Solanum commersonii Dunal, S. chacoense Bitter, Ipomoea grandifolia (Dammer) O’Donell, I. ramosissima (Poir.) Choisy, I. tiliacea (Willd.) Choisy, I. triloba L., and I. cynanchifolia Meisn. The status of the ex situ and in situ conservation of each taxon was assessed using the gap analysis methodology, and the results were used to plan 16 germplasm collection expeditions. Seeds of the collected material were evaluated for viability, and the protocols for seed germination and cryopreservation were tested. The final conservation score, resulting from the gap analysis and including the average of the ex situ and in situ scores, resulted in a classification of medium priority of conservation for all the species, with the exception of I. grandifolia (high priority). The total accessions collected (174) almost doubled the total accessions of these crop wild relatives incorporated in Embrapa’s ex situ conservation system prior to 2015. In addition, accessions for practically absent species were collected for the ex situ conservation system, such as Ipomoea species, Eleusine indica, and Solanum chacoense. The methods used for dormancy breaking and low temperature conservation for the Oryza, Eleusine, and Ipomoea species were promising for the incorporation of accessions in the respective gene banks. The results show the importance of efforts to collect and conserve ex situ crop wild relatives in Brazil based on previous gap analysis. The complementarity with the in situ strategy also appears to be very promising in the country.


Author(s):  
Yapi Elisée Kouakoua ◽  
Aïssatou Coulibaly ◽  
Kouadio Claver Degbeu ◽  
N’Guessan Georges Amani

Aims: The objective of this study was to evaluate the effect of dilution ratio and pasteurization on physico-chemical parameters of Saba senegalensis pulp in the context of its recovery. Study Design: The experimental set-up was of a completely randomized design. Place and Duration of Study: This study was carried out in the Department of Food Science and Technology, at the Laboratory of Food Biochemistry and Tropical Product Technology (BATPTL) of Nangui Abrogoua University, Abidjan, Côte d'Ivoire, between June and August 2019. Methodology: The pulp collected from the fruit was diluted according to the following pulp/water ratio (RPW; v/v): 1:0.5; 1:1 and 1:1.5 and pasteurized at 75°C at different times (5 min, 10 min and 15 min). The pH, titratable acidity (TA), Total soluble solid (TSS), vitamin C, β-carotene and the colour were determined on the different samples. Results: The results obtained during this study showed that all the physico-chemical parameters evaluated, except pH, are affected during dilution while during pasteurization only vitamin C and β-carotene are affected. During the respective dilutions, 50%, 62% and 66% of the vitamin C concentration decreased as well as 53%, 74% and 76% of β-carotene. It also led to a decrease in total soluble solid (TSS) of 32%, 50% and 59%. Following the pasteurization of each diluted pulp12%, 45% and 50% on average of the vitamin C are lost at the respective pasteurization time  (5 min, 10 min and 15 min respectively). For β-carotene the content decreases (50%) within the first 5 minutes and remains constant during the last 10 minutes of pasteurization. Conclusion: During Saba senegalensis fruit processing into nectar, dilution and pasteurization factors must be considered in order to ensure the physico-chemical quality of the beverage. The physico-chemical characteristics were more affected during dilution than in pasteurization.


2016 ◽  
Vol 1 (1) ◽  
pp. 1077-1086
Author(s):  
Niken Wahyuningsih ◽  
Ratna Ratna ◽  
Zulfahrizal Zulfahrizal

Abstrak.Menganalisis mutu selama penyimpanan dapat dilakukan dengan mengendalikan kondisi penyimpanan tertentu serta menduga laju penurunan mutu yang terjadi. Penelitian ini bertujuan untuk menduga umur simpan jeruk siam berdasarkan laju penurunan vitamin C selama penyimpanan menggunakan persamaan Arrhenius. Jeruk siam segar disimpan dengan variasi suhu yaitu suhu 10⁰C, 15⁰C, dan 28⁰C. Analisis dilakukan 3 hari sekali hingga panelis menolak dengan parameter susut bobot, tingkat kekerasan, total padatan terlarut (TPT), vitamin C, dan uji organoleptik yaitu warna, aroma, tekstur, rasa, dan penerimaan keseluruhan. Hasil penelitian diperoleh persentase susut bobot pada suhu 10⁰C dan suhu 15⁰C yaitu 11,57 % pada penyimpanan hari ke- 12. Tingkat kekerasan tertinggi pada suhu penyimpanan 10 ⁰C yaitu 2,09 Kg/cm2. Suhu 15 ⁰C mengalami kenaikan total padatan terlarut paling tinggi hingga 9,70% brix. Uji organoleptik penyimpanan terbaik pada suhu 15 ⁰C dengan lama penyimpanan hingga hari ke- 12, warna, tekstur, aroma, rasa dan penerimaan keseluruhan skornya yaitu 4,28, 4,05, 3,76, 4,50 dan 4,10. Umur simpan jeruk siam pada suhu 10⁰C umur simpannya 15 hari, suhu penyimpanan 15⁰C umur simpannya 14 hari, Suhu  28⁰C umur simpannya 13 hari. Penelitian ini didapatkan model pendugaan laju penurunan mutu jeruk siam selama penyimpanan yaitu k = 30.01808 e-777.54(1/T). Abstract. Analyzing the quality during storage could be done by controlling the storage as well as suspecting the rate of decrease the quality. This research aim to presume the life of siamese orange based on the rate of vitamin C decrease during the storage by using Arrhenius equation. The fresh siamese oranges with variety of storage temperature were 10 0C, 15 0C, and 28  0C. Analysis done in 3 days until the panelist refected to the shrinkage of weight, the level of solid, total soluble solid (TSS), Vitanin C, and organoleptic test such as colour, aroma, texture, taste, acceptance in a whole. From the result it is obtained the percentage of shrinkage weigh of temperature at 10 0C and at 15 0C  was 11,57 % in the storage of day 12. The highest level of solid was on temperature at 10 0C was 2,09 kg/cm2. On the temperature 15 0C it has the total soluble solid up to 9,70b% brix. The best organoleptic test storage was on temperature 15 0C with in 12 days of storage, the highest colour, aroma, texture, taste, and acceptance in a whole score was 4,28, 4,05, 3,76, 4,50 and 4,10. The siamese orange life storage on temperature 10 0C could be storage for 15 days, on temperature 15 0C could be storage for 14 days, on temperature 28 0C could be storage for 13 days. The reseach found out that the persume of decreasing the quality of siamese orange duringstorage was k = 30.01808 e-777.54(1/T).


Author(s):  
François Luro ◽  
Emmanuel Bloquel ◽  
Bruno Tomu ◽  
Gilles Costantino ◽  
Isabelle Tur ◽  
...  

Agriculture ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 14 ◽  
Author(s):  
Danilo Loconsole ◽  
Giuseppe Cristiano ◽  
Barbara De Lucia

Halophytes are naturally adapted in saline environments, where they benefit from the substantial amounts of salt in the growth media. The need for salt-tolerant crops increases as substantial percentages of cultivated land worldwide are affected by salinity. There are few protocols, guidelines, or trials for glasswort (Salicornia (L.) and Sarcocornia (Scott), belong to the Amaranthaceae) field cultivation. The high salt tolerance and content in bioactive compounds make glassworts one of the most important candidates for future use both for fresh and processed food, due to their functional and health properties. This review describes the glassworts respect to their biodiversity and the most important factors affecting propagation, salt tolerance traits, agro-techniques and yields, food uses and nutraceutical properties.


Author(s):  
Liana Mariana Deac ◽  
Anca Farcas ◽  
Dan C. Vodnar ◽  
Maria Tofana ◽  
Sonia Socaci

The fir buds as well as the fir buds extracts (such as fir buds syrup) are used in traditional medicine and phytotheraphy for the alleviation of light to medium forms of respiratory diseases and for the prophylaxis or treatment of some childhood disorders. The antioxidant and antimicrobial properties of the two types fir buds syrups (cold pressed and boiled) taken into study were demonstrated by its content in vitamin C, flavonoids and by the inhibitory effect on E.coli, B. cereus, S.aureus, L. monocytogenes, Salmonella thypi.


Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1457
Author(s):  
Leszek Rydzak ◽  
Zbigniew Kobus ◽  
Rafał Nadulski ◽  
Kamil Wilczyński ◽  
Anna Pecyna ◽  
...  

The paper presents the comparison of quality of six different commercial apple juices produced in Poland. The apple juices came from two different Polish companies. From each manufacturer three various juices were selected: two cloudy and clarified one produced as a mixture of different apple varieties. The following properties were evaluated: density, soluble solid content, viscosity, total phenolic contents, phenolic acid, and antiradical activity. The obtained results showed that three from fourth cloudy juices (M1Sz, M1A and M2A) differed from clarified juices. The highest differences were noticed for total phenolic contents, phenolic acids, antiradical activity and viscosity. The values of these properties ranged between 60.72–103.6 mg GAE·100−1 mL−1 for phenolic contents, 78.3–90.9% for antiradical activity and 2.68–5.79 mPa·s for viscosity. The remaining cloudy juice (M2Sz) was more similar to the clarified ones. The strong correlation between total phenolic content and antiradical activity of apple juices was found. The high correlation coefficient obtained between these features indicates that both producers do not apply excessive amounts of vitamin C during the production of cloudy apple juices. There was also a strong interrelation between the density of juice and solid soluble content. However, no correlation was found between the solid soluble content and the viscosity of the tested juices.


Sign in / Sign up

Export Citation Format

Share Document