scholarly journals Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations

2021 ◽  
Vol 43 ◽  
pp. e55871
Author(s):  
Larissa Pacheco Ferreira ◽  
Erica Cruz ◽  
Meire Lelis Leal Martins
Keyword(s):  
Hydrogen Peroxide ◽  
Bacillus Licheniformis ◽  
Statistical Design ◽  
Cleaning Efficiency ◽  
Component Mixture ◽  
Linear Alkylbenzene ◽  
Bacillus Spp ◽  
Broad Variety

Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a significant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents.

scholarly journals Potencial in vitro de Bacillus spp. no controle de Curtobacterium flaccumfaciens pv. flaccumfaciens em feijoeiro-comum

2016 ◽  
Vol 42 (4) ◽  
pp. 360-362
Author(s):  
Evelynne Urzêdo Leão ◽  
◽  
Júlio Cesar da Silva ◽  
Fabíola Rodrigues Medeiros ◽  
Gabriela Silva Santa Rosa Macêdo ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacillus Licheniformis ◽  
Bacillus Spp

RESUMO O feijoeiro-comum (Phaseolus vulgaris) é uma cultura de grande relevância na alimentação da população brasileira. A murcha-de-curtobacterium ou murcha bacteriana, causada por Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff). é uma doença vascular que acomete o feijoeiro causando danos significativos. Neste contexto, o objetivo deste trabalho foi avaliar a ação in vitro de Bacillus spp. na inibição de dois isolados de Cff, colonização do sistema radicular e desenvolvimento de plântulas de feijoeiro-comum. Foram realizados dois ensaios in vitro para verificar a atividade antagônica dos isolados Bacillus licheniformis, B. subtilis e B. subtilis + B. lichenformis a dois isolados de Cff. Todos os isolados de Bacillus spp. apresentaram inibição no crescimento dos isolados de Cff. Não foi observada a colonização das raízes das plântulas de feijoeiro-comum, pelos isolados bacterianos avaliados.

Bacillus Licheniformis Coated Bioparticles for Hydrogen Peroxide Degradation

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Wei Kheng Teoh ◽  
Zaharah Ibrahim ◽  
Shafinaz Shahir
Keyword(s):  
Hydrogen Peroxide ◽  
Bacillus Licheniformis ◽  
Extracellular Polymeric Substances ◽  
Exponential Phase ◽  
Bacterial Suspension ◽  
Late Exponential Phase ◽  
Repeated Use ◽  
Biofilm Development ◽  
Potential Use ◽  
Second Use

The potential use of Bacillus licheniformis coated bioparticles for hydrogen peroxide (H2O2) degradation was assessed in this study. Bioparticles were made by mixing zeolite, activated carbon and cement in ratio 20:5:6 for attachment of biofilm. The efficiency of H2O2 degradation was examined in the presence and absence of biofilm (control) on bioparticles. Optimisation of biofilm development (7 and 10 days) and reusability were also investigated for H2O2degradation. Actively growing bacterial suspension (late exponential phase) of B.licheniformis was used in development of pure culture biofilm. The 7–day biofilm coated bioparticles system successfully achieved complete H2O2 degradation within an hour (highest rate = 1.17 % H2O2 degraded per minute) while the control showed no significant H2O2 degradation. After repeated use of biofilm coated bioparticles, the rate of H2O2 degradation declined to 0.654 % H2O2degraded per minute, and second use, the rate of H2O2 degradation was 0.166 % H2O2 degraded per minute. Field Emission Scanning Electron Microscope (FESEM) images of the biofilm coated bioparticles showed the attachment of cells and formation of extracellular polymeric substances (EPS), whereas the control showed no biofilm formed.

scholarly journals Investigation of linear alkylbenzene synthesis using nanotitania-supported Dawson heteropolyacid as catalyst by statistical design approaches

2015 ◽  
Vol 42 (4) ◽  
pp. 3283-3301 ◽  
Author(s):  
Nafiseh Toutounchian ◽  
Ali Ahmadpour ◽  
Majid M. Heravi ◽  
Fatemeh F. Bamoharram ◽  
Ali Ayati ◽  
...  
Keyword(s):  
Statistical Design ◽  
Linear Alkylbenzene

scholarly journals An Improved Cleaning Protocol for Foraminiferal Calcite: HyPerCal – A New Practice for Micropaleontological and Paleoclimatic Proxies

2020 ◽  
Author(s):  
Stergios D. Zarkogiannis ◽  
George Kontakiotis ◽  
Georgia Gkaniatsa ◽  
Venkata S. C. Kuppili ◽  
Shashidhara Marathe ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Isotope Composition ◽  
Late Quaternary ◽  
Aegean Sea ◽  
Single Species ◽  
Sea Surface ◽  
Sea Surface Salinity ◽  
Cleaning Efficiency ◽  
Surface Salinity ◽  
Simultaneous Treatment

Paleoclimatic and paleoceanographic studies routinely rely on the usage of foraminiferal calcite through faunal, morphometric and physico-chemical proxies. The application of such proxies presupposes the extraction and cleaning of these biomineralized components from ocean sediments in the most efficient way, a process which is often labor intensive and time consuming. In this respect, in this study we performed a systematic experiment for planktonic foraminiferal specimen cleaning using different chemical treatments and evaluated the resulting data of a Late Quaternary gravity core sample from the Aegean Sea. All cleaning procedures adopted here were made on the basis of their minimum potential bias upon foraminiferal proxies, such as the faunal assemblages, degree of fragmentation, stable isotope composition (&delta;18O and &delta;13C) and/or Mg/Ca ratios that are frequently used as proxies for surface-ocean climate parameters (e.g., sea surface temperature, sea surface salinity). Six different protocols were tested, involving washing, sieving, and chemical treatment of the samples with hydrogen peroxide and/or sodium hexametaphosphate (Calgon &reg;). Single species foraminifera shell weighing was combined with high-resolution Scanning Electron Microscopy (SEM) and synchrotron X-ray Microtomography (S&mu;CT) of the material processed by each of the cleaning protocols, in order to assess the decontamination degree of specimen&rsquo;s ultrastructure and interior. It appeared that a good compromise between time and cleaning efficiency is the simultaneous treatment of samples with a mixed hydrogen peroxide and Calgon solution, while the most effective way for an almost complete decontaminate of the calcareous components from undesirable sedimentary material is a two-step treatment - initially with hydrogen peroxide and subsequently with Calgon solutions.

scholarly journals Natural Endotoxemia in Dogs—A Hidden Condition That Can Be Treated with a Potential Probiotic Containing Bacillus subtilis, Bacillus licheniformis and Pediococcus acidilactici: A Study Model

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1367
Author(s):  
Maria-Catalina Matei ◽  
Sanda Maria Andrei ◽  
Victoria Buza ◽  
Mihai Sorin Cernea ◽  
Daria Antonia Dumitras ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Pediococcus Acidilactici ◽  
Probiotic Potential ◽  
New Information ◽  
Probiotic Treatment ◽  
Before And After ◽  
Bacillus Spp ◽  
Group 2 ◽  
Group 1

Spore-based Bacillus spp. products are considered to have a higher probiotic potential compared to products containing only lactic acid bacteria because their viability in the gastrointestinal (GI) tract is higher, even when GI environmental conditions are unfavorable. The aim of this study was to assess the effect of a Bacillus subtilis, Bacillus licheniformis and Pediococcus acidilactici spore-based potential probiotic on the natural levels of postprandial endotoxemia. A total of 11 dogs completed the study: group 1—healthy dogs: n = 5; group 2—dogs with apparent dysbiosis: n = 6. For 30 days, the dogs were fed the probiotic product; clinical examinations and blood sampling were done before and after completion of the probiotic treatment. Endotoxin levels were assessed pre-meal, 6 h and 12 h post-meal, before initiation and after completion of the treatment. The results showed a decrease in endotoxin levels after treatment, especially 12 h post-meal (group 1: 20.60%; group 2: 44.93%). This study reports new information with regard to natural endotoxemia levels in dogs and suggests that a multi-strain formula (spore-based) consisting of B. subtilis, B. licheniformis and P. acidilactici is able to diminish endotoxin values.

Advanced Aqueous Cleaner II: PER Removal from Sensitive Cu/Low-k Devices

2005 ◽  
Vol 103-104 ◽  
pp. 373-376 ◽  
Author(s):  
Chris Reid ◽  
Jerome Daviot ◽  
Douglas Holmes
Keyword(s):  
Organic Solvent ◽  
Statistical Design ◽  
The Novel ◽  
Environmental Footprint ◽  
Cleaning Efficiency ◽  
Screening Experiment ◽  
Wet Chemical ◽  
Low K ◽  
Etch Residue ◽  
Acidic Region

This paper described the development of two types of Advanced Aqueous Cleaners (AAC™) for Aluminium (Al) Post Etch Residue (PER) removal. The first approach was developed to address a need for cleaning chemistries with a smaller environmental footprint that were also able to clean at significantly lower process times and temperatures than conventional wet chemical cleans. A broad screening experiment was undertaken during which it was highlighted it was possible to clean Al lines in an acidic region though this technology was not extendable to cleaning via features. However, the study emphasised the need to use a selective alkaline reducing formulation to maintain a high cleaning efficiency for the more complex residues formed during via etch. The novel Back End Of Line (BEOL) PER cleaners presented in this paper were optimised using a statistical Design Of Experiment (DOE) to perform at lower temperatures and shorter process times and were Fluoride and organic solvent free while containing a minimum of 80%wt water.

scholarly journals An Improved Cleaning Protocol for Foraminiferal Calcite from Unconsolidated Core Sediments: HyPerCal—A New Practice for Micropaleontological and Paleoclimatic Proxies

2020 ◽  
Vol 8 (12) ◽  
pp. 998
Author(s):  
Stergios D. Zarkogiannis ◽  
George Kontakiotis ◽  
Georgia Gkaniatsa ◽  
Venkata S. C. Kuppili ◽  
Shashidhara Marathe ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Isotope Composition ◽  
Late Quaternary ◽  
Aegean Sea ◽  
Single Species ◽  
Sea Surface ◽  
Sea Surface Salinity ◽  
Cleaning Efficiency ◽  
Surface Salinity ◽  
Simultaneous Treatment

Paleoclimatic and paleoceanographic studies routinely rely on the usage of foraminiferal calcite through faunal, morphometric and physico-chemical proxies. The application of such proxies presupposes the extraction and cleaning of these biomineralized components from ocean sediments in the most efficient way, a process which is often labor intensive and time consuming. In this respect, in this study we performed a systematic experiment for planktonic foraminiferal specimen cleaning using different chemical treatments and evaluated the resulting data of a Late Quaternary gravity core sample from the Aegean Sea. All cleaning procedures adopted here were made on the basis of their minimum potential bias upon foraminiferal proxies, such as the faunal assemblages, degree of fragmentation, stable isotope composition (δ18O and δ13C) and/or Mg/Ca ratios that are frequently used as proxies for surface-ocean climate parameters (e.g., sea surface temperature, sea surface salinity). Six different protocols were tested, involving washing, sieving, and chemical treatment of the samples with hydrogen peroxide and/or sodium hexametaphosphate (Calgon®). Single species foraminifera shell weighing was combined with high-resolution scanning electron microscopy (SEM) and synchrotron X-ray microtomography (SμCT) of the material processed by each of the cleaning protocols, in order to assess the decontamination degree of specimen’s ultrastructure and interior. It appeared that a good compromise between time and cleaning efficiency is the simultaneous treatment of samples with a mixed hydrogen peroxide and Calgon solution, while the most effective way to almost completely decontaminate the calcareous components from undesirable sedimentary material is a two-step treatment—initially with hydrogen peroxide and subsequently with Calgon solutions.

scholarly journals USO DE Bacillus spp. NO CONTROLE DE FITOPATÓGENOS EM SEMENTES DE SOJA VARIEDADE BRS VALIOSA RR

2013 ◽  
Vol 5 (1) ◽  
pp. 68 ◽  
Author(s):  
Gustavo De Andrade Bezerra ◽  
Daniella Aguiar Macedo ◽  
Ivaneide De Oliveira Nascimento ◽  
Thatyane Pereira de Sousa ◽  
Niedja Bezerra Costa ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Bacillus Pumilus ◽  
Bacillus Spp ◽  
E 52

RESUMO: O presente trabalho teve como objetivo avaliar o controle biológico de fitopatógenos da soja realizando microbiolização de sementes com Bacillus spp., B. polymyxa, B. cereus, B. pumilus e B. licheniformis. Para o teste de sanidade foi empregado o método do Blotter Test. Foram utilizadas sementes de soja da variedade BRS Valiosa RR. As sementes foram microbiolizadas com a suspensão de bactérias em solução salina (NaCl 0,85 %), na concentração de OD540 = 0,5 AA e levadas à mesa de agitação por 30 minutos. As sementes foram plaqueadas em papel de filtro em experimentos conduzidos em laboratório com delineamento inteiramente casualizado com seis tratamentos e cinco repetições. Os parâmetros avaliados foram incidência e redução dos patógenos em sementes de soja. Houve a avaliação de sementes sadias e infectadas. A variedade BRS valiosa RR apresentou 48 % de sementes sadias e 52% de sementes infectadas. De acordo com a análise da incidência e controle de fitopatógenos em sementes de soja com uso de Bacillus spp., as espécies Bacillus licheniformis e Bacillus pumilus apresentaram os maiores índices percentuais de controle na variedade utilizada no experimento.   PALAVRAS-CHAVE: controle biológico, microbiolização, sanidade.

scholarly journals A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species

2020 ◽  
Vol 21 (5) ◽  
pp. 1669 ◽  
Author(s):  
Chris R. Taitt ◽  
Tomasz A. Leski ◽  
Amy Chen ◽  
Kimberly L. Berk ◽  
Robert W. Dorsey ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Point Mutations ◽  
Near Neighbor ◽  
Pestis Strain ◽  
High Resolution Melt ◽  
Nucleotide Substitutions ◽  
High Resolution Melt Analysis ◽  
Resistance Determinants ◽  
Bacillus Spp ◽  
Broad Variety

A dramatic increase in global antimicrobial resistance (AMR) has been well documented. Of particular concern is the dearth of information regarding the spectrum and prevalence of AMR within Category A Select Agents. Here, we performed a survey of horizontally and vertically transferred AMR determinants among Category A agents and their near neighbors. Microarrays provided broad spectrum screening of 127 Francisella spp., Yersinia spp., and Bacillus spp. strains for the presence/absence of 500+ AMR genes (or families of genes). Detecting a broad variety of AMR genes in each genus, microarray analysis also picked up the presence of an engineered plasmid in a Y. pestis strain. High resolution melt analysis (HRMA) was also used to assess the presence of quinolone resistance-associated mutations in 100 of these strains. Though HRMA was able to detect resistance-causing point mutations in B. anthracis strains, it was not capable of discriminating these point mutations from other nucleotide substitutions (e.g., arising from sequence differences in near neighbors). Though these technologies are well-established, to our knowledge, this is the largest survey of Category A agents and their near-neighbor species for genes covering multiple mechanisms of AMR.

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