Grafting Collagen on the Plasma Sprayed Titania Coating Treated by Sodium Hydroxide

Author(s):  
Baoe Li ◽  
Xuan Yong Liu ◽  
Chuan Xian Ding

2007 ◽  
Vol 330-332 ◽  
pp. 541-544 ◽  
Author(s):  
Baoe Li ◽  
Xuan Yong Liu ◽  
Chuan Xian Ding

In this work, collagen type I was covalently grafted on the surface of plasma sprayed titania coatings to improve their biocompatibility. The plasma sprayed titania coatings were pretreated by sodium hydroxide to induce the formation of hydroxyl groups which can covalently graft collagen, rendering the collagen having good stability. The dependence of collagen grafting on the sodium hydroxide treatment conditions (concentration, time and temperature) was investigated by measuring the amount of collagen grafted on the titania surface. The biocompatibility of the titania coatings with grafted collagen was evaluated by in vitro cell culture. The results showed that the amount of collagen grafted on the titania coatings increased with the concentration of the sodium hydroxide and the treating temperature, while that on the coating is slightly dependent on the treatment time in sodium hydroxide. In vitro cell culture test proved the positive effects of collagen on the biocompatibility of the plasma sprayed titania coating.



2008 ◽  
Vol 23 (5) ◽  
pp. 1021-1026 ◽  
Author(s):  
Xiao-Bing ZHAO


2021 ◽  
Vol 2021.58 (0) ◽  
pp. E035
Author(s):  
O Mirazul Mahmud Abir ◽  
Yuichi OTSUKA ◽  
Kyuoshi OHNUMA ◽  
Yukio MIYASHITA


2007 ◽  
Vol 83A (4) ◽  
pp. 923-930 ◽  
Author(s):  
Baoe Li ◽  
Xuanyong Liu ◽  
Cong Cao ◽  
Chuanxian Ding


Heliyon ◽  
2019 ◽  
Vol 5 (8) ◽  
pp. e02207
Author(s):  
Sarita Morakul ◽  
Yuichi Otsuka ◽  
Kiyoshi Ohnuma ◽  
Motohiro Tagaya ◽  
Satoshi Motozuka ◽  
...  


2008 ◽  
Vol 202 (14) ◽  
pp. 3221-3226 ◽  
Author(s):  
Xiaobing Zhao ◽  
Xuanyong Liu ◽  
Jing You ◽  
Zhigang Chen ◽  
Chuanxian Ding


2001 ◽  
Vol 139 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Hyung-Jun Kim ◽  
Chang-Hee Lee ◽  
Young-Gak Kweon


2021 ◽  
Author(s):  
Mirazul Mahmud Abir ◽  
Yuichi Otsuka ◽  
Yukio Miyashita

Abstract This study investigates the effect of composition on the antibacterial and antiviral properties of hydroxyapatite/titania composite coatings deposited by suspension plasma spraying. Hydroxyapatite is a bioceramic material used as a plasma-sprayed coating to promote osseointegration of femoral stems. TiO2 has promising photocatalytic activity and good efficiency in destroying bacteria, viral species, and parasites. Prior to coating, substrates were grit blasted, ultrasonically cleaned, and heated to enhance adhesion strength. The microstructure of the resulting coatings was then characterized using XRD and Raman spectroscopy. Test results indicated that SPS transformed Ti2O3 into TiO2 with mixed phases. Ti4O7 and Ti3O5 phases were also identified, which show photocatalytic activity due to oxygen vacancies. Antibacterial and antiviral tests were conducted as well.



2006 ◽  
Vol 200 (18-19) ◽  
pp. 5487-5492 ◽  
Author(s):  
Xiaobing Zhao ◽  
Xuanyong Liu ◽  
Chuanxian Ding ◽  
Paul K. Chu


Author(s):  
Russell L. Steere ◽  
Eric F. Erbe

Thin sheets of acrylamide and agar gels of different concentrations were prepared and washed in distilled water, cut into pieces of appropriate size to fit into complementary freeze-etch specimen holders (1) and rapidly frozen. Freeze-etching was accomplished in a modified Denton DFE-2 freeze-etch unit on a DV-503 vacuum evaporator.* All samples were etched for 10 min. at -98°C then re-cooled to -150°C for deposition of Pt-C shadow- and C replica-films. Acrylamide gels were dissolved in Chlorox (5.251 sodium hypochlorite) containing 101 sodium hydroxide, whereas agar gels dissolved rapidly in the commonly used chromic acid cleaning solutions. Replicas were picked up on grids with thin Foimvar support films and stereo electron micrographs were obtained with a JEM-100 B electron microscope equipped with a 60° goniometer stage.Characteristic differences between gels of different concentrations (Figs. 1 and 2) were sufficiently pronounced to convince us that the structures observed are real and not the result of freezing artifacts.



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