Study on Screening Microorganisms with Resistance to Parathion-Methyl and Degradation Characteristics

2012 ◽  
Vol 209-211 ◽  
pp. 2077-2080
Author(s):  
Ping Fang ◽  
Lin Lin Wang ◽  
Xue Yu Zhang ◽  
Lie Ti Sha Wu ◽  
Bin Hui Jiang
Keyword(s):  
Organophosphorus Pesticide ◽  
Bacillus Pasteurii ◽  
16S Rdna Gene ◽  
Gene Sequence Analysis ◽  
Bacterial Morphology ◽  
Eighth Edition ◽  
Biochemical Experiment ◽  
Physiological And Biochemical ◽  
Main Factor

Toxicity of organophosphorus pesticide and the use of non-standard, which was the main factor of soil pesticides pollution, directly or indirectly harm human health. This study screened three wild species of bacteria, from the soil contaminated by pesticides in long-term, and respectively name as A1, A2 and A3. According to the bacterial 16S rDNA gene sequence analysis, combining with bacterial morphology, physiological and biochemical experiment results, and referring to "Berger bacterial identification manuals (eighth edition)", identified the three strains as Bacillus Branch, Clostridium microorganisms. A1, A2 and A3 were respectively Bacillus pasteurii, Clostridium butyricum and Clostridium acetobutylicum. Through the strain’s degradation and tolerability experiment to parathion-methyl, it turned out that the three strains reached the highest degradation rate in 12h, which was 73.04%, 78.05% and 85.51%, respectively.

Apophysites – Revue de la littérature et ­nouvelles directions

2014 ◽  
Vol 62 (3) ◽  
Keyword(s):  
Scientific Evidence ◽  
Ossification Center ◽  
Health Staff ◽  
Optimal Management ◽  
Long Term Effect ◽  
High Incidence ◽  
Youth Athlete ◽  
Pathology Education ◽  
Main Factor

Apophysitis are part of the growth-related diseases within youth athlete population. Despite their high incidence within this growing cohort, many doubts remain. The physiopathology is still debated. Initially, the fragmentation of the ossification center was seen as the main factor of the disease. For few years, this theory has been questioned due to consistent signs of tendon suffering. Apophysitis may have some negative long-term effect on a sporting career. There is currently poor scientific evidence on the optimal management and no treatment has been widely accepted. Prevention remains the most powerful intervention in this particular pathology. Education of the athlete’s sporting entourage (family, coaches and health staff) and the athlete himself is necessary to act quickly and adapt the training load to decrease mechanical stress on the suffering apophysis.

scholarly journals Thickness and corrosion resistance optimization of Micro-ARC oxidation coating on Mg-Al-Li-Zn alloy using Taguchi approach

2020 ◽  
pp. 62-66
Author(s):  
Do Le Hung Toan, Shuo-Jen Lee Do
Keyword(s):  
Corrosion Resistance ◽  
Processing Time ◽  
Electrical Potential ◽  
Surface Protection ◽  
Alkaline Electrolytes ◽  
Micro Arc Oxidation ◽  
Corrosive Environments ◽  
Zn Alloy ◽  
Main Factor

Micro arc oxidation method has been developed in the field of surface protection of magnesium alloys and considered as a simple, highly effective, commercial and environmentally friendly method in industry. MAO coatings are fabricated on novel Mg-Al-Li-Zn alloy to improve the anti-corrosion performance of surface by using friendly alkaline electrolytes under a high electrical potential. The Taguchi method and optimal analysis are used to identify the effects of the three factors including current density, processing time and electrical frequency on coating’s characteristics. The results have shown that the main factor that affects coating thickness and corrosion resistance of coating is the processing time. The results obtained by optimal conditions are consistent with prediction values of Taguchi analysis. The thickness of the coating can help to improve the long-term corrosion protection of a MAO coating in corrosive environments.

scholarly journals Metabolic responses of Carlina acaulis L. to chronic and acute cadmium stress: insights into chelation mechanisms, non-enzymatic antioxidants, and specialized metabolism

2020 ◽  
Author(s):  
Sławomir Dresler ◽  
Maciej Strzemski ◽  
Jozef Kováčik ◽  
Jan Sawicki ◽  
Michał Staniak ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Cadmium Stress ◽  
Glutathione Depletion ◽  
Metabolic Responses ◽  
Shoot Biomass ◽  
Abiotic Factor ◽  
Enzymatic Antioxidants ◽  
Short Term ◽  
Physiological And Biochemical

Abstract BackgroundChronic and acute stress can lead to completely different metabolic responses of plants exposed to the same abiotic factor. The effect of long-term chronic cadmium stress (ChS, 0.1 µM Cd, 85 days) or short-term acute cadmium stress (AS, 10 µM Cd, 4 days) on the physiology of Carlina acaulis L. (Asteraceae) and selected secondary metabolites was compared to identify specific physiological and biochemical reactions.ResultsThe bioconcentration of Cd in all analyzed organs was higher under AS in comparison with ChS (130 vs. 16 µg g-1 DW, 7.9 vs. 3.2 µg g-1 DW, and 11.5 vs. 2.4 µg g-1 DW in roots, leaves, and trichomes, respectively). The high concentration of Cd in the trichomes in the AS treatment might be an anatomical adaptation mechanism. ChS evoked an increase in the root biomass, whereas its impact on shoot biomass was not significant in any treatment. The amounts of ascorbic acid and the sum of phytochelatins were higher in the shoots, whilst organic acids (malic and citric) reached higher levels in the roots of plants from the ChS treatment. Glutathione depletion occurred in the shoots, but there was no change in its root level in both treatments. The amount of chlorogenic acid, but not that of ursolic and oleanolic acids, was increased under ChS. On the other hand, AS exposure elevated the level of ursolic and oleanolic acids, but not chlorogenic acid in the shoots. ConclusionsThese data indicate that ChS and AS induce different physiological and biochemical defense mechanisms. Both chelation and enhancement of the antioxidative machinery contribute to protection of C. acaulis exposed to long-term (chronic) Cd exposure and alleviate Cd toxicity effectively. However, triterpene acids were elevated only under AS treatment, which may suggest an instantaneous action of these metabolites under short-term acute Cd stress.

Identification and characterization of a cold-adapted and halotolerant nitrobenzene-degrading bacterium

RSC Advances ◽  
2015 ◽  
Vol 5 (98) ◽  
pp. 80276-80282 ◽  
Author(s):  
Xiping Ma ◽  
Xianzhu Li ◽  
Wanlong Li ◽  
Di Wang ◽  
Chengbin Xu ◽  
...  
Keyword(s):  
16S Rdna ◽  
Gene Sequence ◽  
16S Rdna Gene ◽  
Cold Adapted ◽  
Biochemical Characteristics ◽  
Physiological And Biochemical Characteristics ◽  
Degrading Bacterium ◽  
Identification And Characterization ◽  
Physiological And Biochemical

According to strain X7's morphological, physiological and biochemical characteristics and 16S rDNA gene sequence, the result showed that strain X7 was Myroides odoratus.

scholarly journals Stenotrophomonas terrae sp. nov. and Stenotrophomonas humi sp. nov., two nitrate-reducing bacteria isolated from soil

2007 ◽  
Vol 57 (9) ◽  
pp. 2056-2061 ◽  
Author(s):  
Kim Heylen ◽  
Bram Vanparys ◽  
Filip Peirsegaele ◽  
Liesbeth Lebbe ◽  
Paul De Vos
Keyword(s):  
Soil Analysis ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Gene Sequence Analysis ◽  
Species Analysis ◽  
Different Strains ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria ◽  
Type Strains ◽  
Physiological And Biochemical

Three Gram-negative, rod-shaped, non-spore-forming, nitrate-reducing isolates (R-32746, R-32768T and R-32729T) were obtained from soil. Analysis of repetitive sequence-based PCR showed that the three isolates represented two different strains. 16S rRNA gene sequence analysis and DNA–DNA hybridization placed them within the genus Stenotrophomonas and revealed that they were genotypically different from each other and from all recognized Stenotrophomonas species. Analysis of the fatty acid composition and physiological and biochemical tests allowed differentiation from their closest phylogenetic neighbours. They are therefore considered to represent two novel species, for which the names Stenotrophomonas terrae sp. nov. and Stenotrophomonas humi sp. nov. are proposed, with strains R-32768T (=LMG 23958T=DSM 18941T) and R-32729T (=LMG 23959T=DSM 18929T), respectively, as the type strains.

scholarly journals Brassinosteroid Priming Improves Peanut Drought Tolerance via Eliminating Inhibition on Genes in Photosynthesis and Hormone Signaling

Genes ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 919
Author(s):  
Luping Huang ◽  
Lei Zhang ◽  
Ruier Zeng ◽  
Xinyue Wang ◽  
Huajian Zhang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Priming ◽  
Growth And Yield ◽  
Signal Cascade ◽  
Growth Promoting ◽  
Fast Adaptation ◽  
Gene Ontologies ◽  
Transcriptomic Changes ◽  
Physiological And Biochemical

Drought negatively affects the growth and yield of terrestrial crops. Seed priming, pre-exposing seed to a compound, could induce improved tolerance and adaptation to stress in germinated plants. To understand the effects and regulatory mechanism of seed priming with brassinosteroid (BR) on peanut plants, we treated seeds with five BR concentrations and examined dozens of physiological and biochemical features, and transcriptomic changes in leaves under well-watered and drought conditions. We found optimal 0.15 ppm BR priming could reduce inhibitions from drought and increase the yield of peanut, and priming effects are dependent on stage of plant development and duration of drought. BR priming induced fewer differentially expressed genes (DEGs) than no BR priming under well-watered condition. Drought with BR priming reduced the number of DEGs than drought only. These DEGs were enriched in varied gene ontologies and metabolism pathways. Downregulation of DEGs involved in both light perceiving and photosynthesis in leaves is consistent with low parameters of photosynthesis. Optimal BR priming partially rescued the levels of growth promoting auxin and gibberellin which were largely reduced by drought, and increased levels of defense associated abscisic acid and salicylic acid after long-term drought. BR priming induced many DEGs which function as kinase or transcription factor for signal cascade under drought. We proposed BR priming-induced regulatory responses will be memorized and recalled for fast adaptation in later drought stress. These results provide physiological and regulatory bases of effects of seed priming with BR, which can help to guide the framing improvement under drought stress.

scholarly journals Long-term seasonal and spatial patterns in mortality and survival of Calanus finmarchicus across the Atlantic Zone Monitoring Programme region, Northwest Atlantic

2009 ◽  
Vol 66 (9) ◽  
pp. 1942-1958 ◽  
Author(s):  
Stéphane Plourde ◽  
Pierre Pepin ◽  
Erica J. H. Head
Keyword(s):  
Population Growth ◽  
Spatial Patterns ◽  
Phytoplankton Biomass ◽  
Mortality Rates ◽  
Monitoring Programme ◽  
Calanus Finmarchicus ◽  
Northwest Atlantic ◽  
Specific Mortality ◽  
Main Factor

Abstract Plourde, S., Pepin, P., and Head, E. J. H. 2009. Long-term seasonal and spatial patterns in mortality and survival of Calanus finmarchicus across the Atlantic Zone Monitoring Programme region, Northwest Atlantic. – ICES Journal of Marine Science, 66: 1942–1958. The vertical life table method was used to estimate stage-specific daily mortality rates and survival from 1999 to 2006 for Calanus finmarchicus sampled in the Canadian Atlantic Zone Monitoring Programme, which covers the Newfoundland–Labrador Shelf (NLS), Gulf of St Lawrence (GSL), and Scotian Shelf (SS). Stage-specific mortality rates and survival showed significant regional and seasonal differences, with the largest signal associated with variations in temperature. Density-dependent mortality, associated with the abundance of C6 females, was the main factor influencing mortality in the egg–C1 transition during the period of population growth in spring on the SS, and in summer in the GSL and on the NLS. In autumn, mortality in egg–C1 was positively related to temperature and negatively related to phytoplankton biomass, with particularly high mortality rates on the SS. The integration of our results into stage-specific recruitment rates from egg to C5 revealed that C. finmarchicus populations experience their greatest loss (mortality) during the egg–C1 transition. Loss during development to C1 was greater in the GSL than in the other regions during the period of population growth, resulting in lower recruitment success in the GSL. In autumn, C. finmarchicus showed low stage-specific daily recruitment rates on the SS at high temperatures, and low phytoplankton biomass compared with those in the GSL and on the NLS. Our findings reinforce the necessity of describing regional and seasonal patterns in mortality and survival to understand factors controlling the population dynamics of C. finmarchicus.

scholarly journals Microlunatus soli sp. nov., isolated from soil

2010 ◽  
Vol 60 (4) ◽  
pp. 824-827 ◽  
Author(s):  
P. Kämpfer ◽  
Chiu-Chung Young ◽  
H.-J. Busse ◽  
Jiunn-Nan Chu ◽  
P. Schumann ◽  
...  
Keyword(s):  
Edible Mushroom ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Glycine Residue ◽  
Gene Sequence Analysis ◽  
Peptidoglycan Structure ◽  
Unknown Phospholipid ◽  
Type Strains ◽  
Physiological And Biochemical

A Gram-stain-positive, coccoid, non-endospore-forming actinobacterium (strain CC-12602T) was isolated from a spawn used for growing the edible mushroom Agaricus brasiliensis in the laboratory. On the basis of 16S rRNA gene sequence analysis, strain CC-12602T was shown to belong to the genus Microlunatus and was related most closely to the type strains of Microlunatus ginsengisoli (96.1 % similarity), M. phosphovorus (95.9 %), M. panaciterrae (95.8 %) and M. aurantiacus (95.5 %). The quinone system comprised menaquinone MK-9(H4) as the major component and the polyamine pattern consisted of spermidine and spermine as major compounds. The predominant polar lipids were phosphatidylglycerol and unknown phospholipid PL3. Moderate amounts of diphosphatidylglycerol, an unknown glycolipid and three unknown phospholipids and minor amounts of an unknown phospholipid and a polar lipid were detected. The peptidoglycan type was A3γ′, based on ll-2,6-diaminopimelic acid with an interpeptide bridge consisting of a single glycine residue and a second glycine residue at position 1 of the peptide subunit. Peptidoglycan structure and major fatty acids (anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0) supported the affiliation of strain CC-12602T to the genus Microlunatus. The results of physiological and biochemical tests allowed strain CC-12602T to be differentiated phenotypically from recognized Microlunatus species. Strain CC-12602T is therefore considered to represent a novel species of the genus Microlunatus, for which the name Microlunatus soli sp. nov. is proposed. The type strain is CC-12602T (=DSM 21800T =CCM 7685T).

Indole degrading of ammonia oxidizing bacteria isolated from swine wastewater treatment system

2009 ◽  
Vol 59 (12) ◽  
pp. 2405-2410 ◽  
Author(s):  
Ping Li ◽  
Lei Tong ◽  
Kun Liu ◽  
Yanhong Wang ◽  
Yanxin Wang
Keyword(s):  
Wastewater Treatment ◽  
Biochemical Analysis ◽  
Ammonia Oxidation ◽  
Treatment System ◽  
Swine Wastewater ◽  
Ammonia Oxidizing Bacteria ◽  
16S Rdna Gene ◽  
Wastewater Treatment System ◽  
Nitrate And Nitrite ◽  
Physiological And Biochemical

Three new strains named LPA11, LPB11 and LPC24 were isolated to investigate the patterns of indole degradation and ammonia oxidation in swine wastewater from different parts of a swine wastewater treatment system by the direct spreading plate method. These three isolates were all identified as Pseudomonas putida based on 16S-rDNA gene sequences, main physiological and biochemical analysis. They were capable of decomposing 1.0 mM indole completely in 10, 16 and 18 days respectively. According to the results of HPLC and GC/MS, the possible pathway for the degradation was via oxindole, isatin and anthranilic acid. The three bacteria were capable of oxidizing ammonia, and the strains LPA11 and LPC24 were capable of effectively reducing nitrate and nitrite.

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