Mechanism of Hyaluronic Acid Protect Rabbit Chondrocyte Apoptosis Induced by NO

2013 ◽  
Vol 295-298 ◽  
pp. 78-81
Author(s):  
Hua Liu ◽  
Hua Guang Li ◽  
Su Liu
Keyword(s):  
Hyaluronic Acid ◽  
Treated Group ◽  
Calcium Overload ◽  
Intracellular Free Calcium ◽  
Chondrocyte Apoptosis ◽  
Free Calcium ◽  
Model Group ◽  
Culture Experiment ◽  
Cell Culture Experiment

AIM: To investigate the mechanism of hyaluronic acid on rabbit chondrocyte apoptosis in vitro induced by NO. METHODS: We cultured rabbit chondrocytes as normal group and added SNP after cultured 24 h as model group. Treated group was added HA. We used cell culture experiment. We tested the activity of mitochondria though MTT. The flow cytometry detected mitochondrial membrane potential, the percentage of apoptosis and intracellular free calcium concentration.RESULTS: HA could elevate the active of chondrocyte mitochondria and MMP; it could decrease the rate of chondrocyte apoptosis and intracellular free calcium concentration.CONCLUSION: HA can inhibit the lowering of the MMP in chondrocyte, which has a stable role on MMP and inhibit apoptosis occurred. This effect may be related to inhibiting of intracellular calcium overload chondrocytes.

scholarly journals Preclinical Evaluation and Monitoring of the Therapeutic Response of a Dual Targeted Hyaluronic Acid Nanodrug

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Minglong Chen ◽  
Wenqi Zhang ◽  
Kai Yuan ◽  
Mingxiang Bo ◽  
Bin Chen ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Therapeutic Response ◽  
Drug Carrier ◽  
Treated Group ◽  
Great Promise ◽  
Positron Emission ◽  
Evaluation And Monitoring ◽  
Chemotherapy Agents ◽  
Xenograft Tumor Growth

Chemotherapy is a powerful cancer treatment but suffers from poor biocompatibility and a lack of tumor targeting. Here, we developed a CD44-targeted polymeric nanocomplex by encapsulating 10-hydroxycamptothecin (HCPT) into hyaluronic acid nanoparticles (HANP) for targeted cancer therapy. In vitro, the HANP/HCPT showed improved cytotoxicity to five cancer cell lines including HT29, A549, MDA-MB-231, HepG2, and MDA-MB-435 versus free HCPT. After systemic administration into MDA-MB-231 breast cancer xenograft, tumor growth was significantly inhibited 5.25 ± 0.21 times in the HANP/HCPT treated group relative to the nontreated group. In addition, the treatment response was also accessed and confirmed by 18F-fluoro-2-deoxy-D-glucose ([18F] FDG) positron emission tomography (PET). The MDA-MB-231 tumors responded to HANP/HCPT 7 days after the first treatment, which benefits treatment strategy adjustment and personalization. No apparent systemic toxic effects were seen in mice treated with HANP/HCPT. In summary, the HANPs have great promise as a targeted drug carrier for cancer chemotherapy. Our HANP platform can also deliver other hydrophobic chemotherapy agents.

scholarly journals Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Xiao-hong Du ◽  
Qing-jun Chen ◽  
Jian-bo Song ◽  
Yan Xie ◽  
Yan Zhi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Calcium Ion ◽  
Lipid Peroxide ◽  
Intestinal Injury ◽  
Intracellular Free Calcium ◽  
Free Calcium ◽  
Stress Injury ◽  
Oxidative Stress Injury

Rhubarb-Aconite Decoction (RAD), a famous Chinese medicine prescription, has been widely used for treating intestinal injury. However, the effect of RAD on intestinal epithelial cells is unclear. The aim of this study was to investigate the effects of RAD drug-containing serum on the oxidative stress injury and inflammatory response induced by endotoxin (ET) in Caco-2 cells in vitro. Lipid peroxide malondialdehyde (MDA), lactate dehydrogenase (LDH), caspase-11, tumor necrosis factor-α(TNF-α), interleukin-3(IL-3), and cytokeratin (CK)18, adenosine triphosphate (ATP) activity, and intracellular free calcium ion levels were measured. The results showed that ET triggered the activation of caspase-11 and the massive release of TNF-α, increased the inhibitory rate of cell growth, MDA, and LDH expressions in Caco-2 cells. Moreover, RAD drug-containing serum could inhibit caspase-11 activation, decrease the release of TNF-α and IL-3, reduce intracellular free calcium ion, and enhance CK 18 expression and ATP activity. These novel findings demonstrated that ET-induced oxidative stress injury and inflammatory response of Caco-2 cells were improved by RAD drug-containing serum, indicating that RAD may be a good choice for the treatment of intestinal injury.

Diencephalic GABAergic Neurons in vitro Respond to Prolactin with a Rapid Increase in Intracellular Free Calcium

1992 ◽  
Vol 56 (2) ◽  
pp. 148-152 ◽  
Author(s):  
Walter Kolbinger ◽  
Cordian Beyer ◽  
Karl Föhr ◽  
Ingrid Reisert ◽  
Christof Pilgrim
Keyword(s):  
Gabaergic Neurons ◽  
Intracellular Free Calcium ◽  
Free Calcium

The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes

2016 ◽  
Vol 28 (5) ◽  
pp. 599 ◽  
Author(s):  
Jason R. Herrick ◽  
Chunmin Wang ◽  
Zoltan Machaty
Keyword(s):  
Enzymatic Digestion ◽  
Blastocyst Stage ◽  
Intracellular Free Calcium ◽  
In Vitro Fertilisation ◽  
Free Calcium ◽  
Blastocyst Development ◽  
Oocyte Vitrification ◽  
Developmental Potential ◽  
Parthenogenetic Activation

Embryos produced from vitrified feline oocytes have resulted in pregnancies, but the efficiency of oocyte vitrification in cats is still low. Our objectives were to evaluate the effects of exposing feline oocytes to ethylene glycol (EG), propanediol (PrOH) and dimethyl sulfoxide (DMSO) on changes in intracellular free-calcium concentrations ([Ca2+]i), the time needed for enzymatic digestion of the zona pellucida (ZP), the incidence of parthenogenetic activation and degeneration and embryonic development following in vitro fertilisation (IVF). All of the chemicals tested altered [Ca2+]i, but changes in [Ca2+]i, resistance of the ZP to enzymatic digestion and the incidence of parthenogenetic activation (<5% for all treatments) were not affected (P > 0.05) by extracellular Ca2+. Exposure to EG (>44.1%) and DMSO (19.7%) increased (P < 0.05) oocyte degeneration compared with control oocytes and oocytes exposed to PrOH (≤2.5%). Following exposure to a combination of PrOH and DMSO (10% v/v each), blastocyst development (per cleaved embryo; 52.1%) was similar (P > 0.05) to control oocytes (64.4%). When oocytes were vitrified with PrOH and DMSO, 28.3% of surviving (intact plasma membrane) oocytes cleaved following IVF, but no blastocyst developed. When a non-permeating cryoprotectant (galactose, 0.25 M) was added to the vitrification medium, 47.7% of surviving oocytes cleaved and 14.3% developed to the blastocyst stage.

scholarly journals Enhanced Intracellular Uptake of CdTe Quantum Dots by Conjugation of Oligopeptides

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Moon-Jeong Choi ◽  
R. Pierson ◽  
Yongmin Chang ◽  
Haiquing Guo ◽  
Inn-Kyu Kang
Keyword(s):  
Quantum Dots ◽  
Intracellular Uptake ◽  
3T3 Cells ◽  
Culture Experiment ◽  
Mean Size ◽  
Cdte Quantum Dot ◽  
Nih 3T3 ◽  
Cell Culture Experiment ◽  
Nih 3T3 Cells

Arg-Gly-Asp-Ser (RGDS), a typical membrane-permeable carrier peptide, was conjugated with mercaptoisobutyric acid-immobilized CdTe quantum dot (CTNPs) to enhance the intracellular uptake of quantum dots. Mean size of mercaptoisobutyric acid-immobilized quantum dots (37 nm) as determined by dynamic light scattering was increased up to 54 nm after RGDS immobilization. It was found, fromin vitrocell culture experiment, that fibroblast (NIH 3T3) cells were well proliferated in the presence of RGDS-conjugated quantum dots (RCTNPs), and the intracellular uptake of CTNPs and RCTNPs was studied by means of ICP and fluorescence microscopy. As a result, the RCTNPs specifically bound to the membrane of NIH 3T3 cells and almost saturated after 6 hours incubation. The amount of RCTNPs uptaken by the cells was higher than that of CTNPs, demonstrating the enhancing effect of RGDS peptide conjugation on the intracellular uptake of quantum dots (QDs).

scholarly journals Bryophyllum pinnatum Compounds Inhibit Oxytocin-Induced Signaling Pathways in Human Myometrial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Stefanie Santos ◽  
Leonie Zurfluh ◽  
Mónica Mennet ◽  
Olivier Potterat ◽  
Ursula von Mandach ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Calcium Concentration ◽  
Inhibitory Effect ◽  
Intracellular Free Calcium ◽  
Free Calcium ◽  
Myometrial Cells ◽  
Intracellular Free Calcium Concentration ◽  
Free Calcium Concentration ◽  
Bryophyllum Pinnatum

Bryophyllum pinnatum has been used in the treatment of premature labor, first in anthroposophic hospitals and, recently, in conventional settings as an add-on medication. In vitro work with hTERT human myometrial cells showed that B. pinnatum leaf press juice inhibits the increase of intracellular free calcium concentration induced by oxytocin, a hormone known to play a role in labor. Our aim was to identify fractions/compounds in B. pinnatum press juice that contribute to this inhibitory effect, and to investigate their effect on oxytocin-driven activation of the MAPK cascade. Several fractions/compounds from B. pinnatum press juice led to a concentration-dependent decrease of oxytocin-induced increase of intracellular free calcium concentration, but none of them was as strong as B. pinnatum press juice. However, the combination of a bufadienolide and a flavonoid-enriched fraction was as effective as B. pinnatum press juice, and their combination had a synergistic effect. B. pinnatum press juice inhibited oxytocin-driven activation of MAPKs SAPK/JNK and ERK1/2, an effect also exerted by the bufadienolide-enriched fraction. The effect of B. pinnatum press juice on oxytocin-induced signaling pathways was comparable to that of the oxytocin-receptor antagonist and tocolytic agent atosiban. Our findings further substantiate the use of B. pinnatum press juice preparations in the treatment of preterm labor.

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