Handheld, One-Step, and Rapid Electrochemical Biosensor Platform with Smartphone Interface

2017 ◽  
Vol 868 ◽  
pp. 340-344 ◽  
Author(s):  
Jin An Wang ◽  
Zhong Liang Xia ◽  
Yan Su ◽  
Min Cong Lu ◽  
Ying Wan ◽  
...  
Keyword(s):  
Screen Printing ◽  
Electrochemical Biosensor ◽  
Point Of Care ◽  
Detection Time ◽  
Maximum Residue Limit ◽  
Point Of Care Testing ◽  
Step Detection ◽  
Electrode Sensor ◽  
One Step ◽  
User Friendly

Organophosphorous pesticide (OP), one of the most commonly used pesticides, could have serious adverse effects on human health and the environment. As the maximum residue limit (MRL) of OPs in food and water is low, it is in great demand to develop integrated, portable, user-friendly devices for the rapid and sensitive detection of OP residue. This work aims to present a homemade handhold device and screen printing electrode (SPE) chip for rapid, sensitive, and one-step detection of OPs. A handhold device was designed and fabricated to connect with smartphone to readout the electrochemical signals. Taking advantage of a three-electrode sensor chip and the inhibiting reaction of acetylcholine esterase (AchE), Trichlorphon can be detected in a considerable low detection limit of 1 ng/mL. By using the as-fabricated device and chip, the detection time was only 5 min and each test costs less than $1. The platform will allow point-of-care testing (POCT) of OPs and other targets, which shows promise for healthy and environmental monitoring.

scholarly journals Disposable Voltammetric Immunosensors Integrated with Microfluidic Platforms for Biomedical, Agricultural and Food Analyses: A Review

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4124 ◽  
Author(s):  
Fabiana Felix ◽  
Alexandre Baccaro ◽  
Lúcio Angnes
Keyword(s):  
Quality Management ◽  
Signal Amplification ◽  
Screen Printing ◽  
Point Of Care ◽  
Microfluidic Devices ◽  
Strong Interactions ◽  
Point Of Care Testing ◽  
Microfluidic Platforms ◽  
Broad Variety ◽  
Interesting Alternative

Disposable immunosensors are analytical devices used for the quantification of a broad variety of analytes in different areas such as clinical, environmental, agricultural and food quality management. They detect the analytes by means of the strong interactions between antibodies and antigens, which provide concentration-dependent signals. For the herein highlighted voltammetric immunosensors, the analytical measurements are due to changes in the electrical signals on the surface of the transducers. The possibility of using disposable and miniaturized immunoassays is a very interesting alternative for voltammetric analyses, mainly, when associated with screen-printing technologies (screen-printed electrodes, SPEs), and microfluidic platforms. The aim of this paper is to discuss a carefully selected literature about different examples of SPEs-based immunosensors associated with microfluidic technologies for diseases, food, agricultural and environmental analysis. Technological aspects of the development of the voltammetric immunoassays such as the signal amplification, construction of paper-based microfluidic platforms and the utilization of microfluidic devices for point-of-care testing will be presented as well.

scholarly journals Point-of-Care Testing-the Key in the Battle against SARS-CoV-2 Pandemic

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1464
Author(s):  
Florina Silvia Iliescu ◽  
Ana Maria Ionescu ◽  
Larisa Gogianu ◽  
Monica Simion ◽  
Violeta Dediu ◽  
...  
Keyword(s):  
Clinical Decision Making ◽  
Point Of Care ◽  
Low Cost ◽  
Diagnostic Testing ◽  
Clinical Decision ◽  
Rapid Screening ◽  
Diagnostic Tools ◽  
Point Of Care Testing ◽  
Qrt Pcr ◽  
User Friendly

The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the “gold standard” involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.

Integrating Target-Responsive Hydrogel with Pressuremeter Readout Enables Simple, Sensitive, User-Friendly, Quantitative Point-of-Care Testing

2017 ◽  
Vol 9 (27) ◽  
pp. 22252-22258 ◽  
Author(s):  
Dan Liu ◽  
Shasha Jia ◽  
Huimin Zhang ◽  
Yanli Ma ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Testing ◽  
User Friendly

Blocking-free and self-contained immunoassay platform for one-step point-of-care testing

2020 ◽  
Vol 165 ◽  
pp. 112394 ◽  
Author(s):  
Qi Liu ◽  
Xiaohu Zhou ◽  
Han Wu ◽  
Bo Zheng
Keyword(s):  
Point Of Care ◽  
Point Of Care Testing ◽  
One Step

A Distance-based Capillary Biosensor Using Wettability Alteration

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yansheng Li ◽  
Xiujin Men ◽  
Guowei Gao ◽  
Ye Tian ◽  
Yongqiang Wen ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Wettability Alteration ◽  
Detection Methods ◽  
Point Of Care Testing ◽  
User Friendly

Distance-based detection methods with quantitative readout are of great significance to point-of-care testing (POCT), which are low-cost, user-friendly and can be integrated into portable analytical devices. Here, we submit a...

scholarly journals Portable RT-PCR system: A Rapid and Scalable Diagnostic Tool for COVID-19 Testing

2021 ◽  
Author(s):  
Hosam M Zowawi ◽  
Thamer H Alenazi ◽  
Waleed S AlOmaim ◽  
Ahmad Wazzan ◽  
Abdullah Alsufayan ◽  
...  
Keyword(s):  
Point Of Care ◽  
Rapid Test ◽  
Standard Of Care ◽  
Test Results ◽  
Rt Pcr ◽  
Point Of Care Testing ◽  
System A ◽  
Portable System ◽  
Remote Sites ◽  
User Friendly

Combating the ongoing coronavirus disease 2019 (COVID-19) pandemic demands accurate, rapid, and point-of-care testing with fast results to triage cases for isolation and treatment. The current testing relies on RT-PCR, which is routinely performed in well-equipped laboratories by trained professionals at specific locations. However, during busy periods high numbers of samples queued for testing can delay the test results, impacting upon efforts to reduce the infection risk. Besides, the absence of well-established laboratories at remote sites and low-resourced environments can contribute to a silent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). These reasons compel the need to accommodate point-of-care testing for COVID-19 that meets the ASSURED criteria (Affordable, Sensitive, Specific, User-friendly, Rapid, robust, Equipment-free, and Deliverable). This study assessed the agreement and accuracy of the portable Biomeme SARS-CoV-2 system against the gold standard tests. Nasopharyngeal and nasal swabs were used. Of the 192 samples tested using the Biomeme SARS-CoV-2 system, the results from 189 samples (98.4%) were in agreement with the reference standard-of-care RT-PCR testing for SARS-CoV-2. The portable system generated simultaneous results for nine samples in 80 mins with high positive and negative percent agreements of 99.0% and 97.8%, respectively. We performed separate testing in a sealed glove box, offering complete biosafety containment. Thus, the Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote and low-resourced settings. Single Sentence Summary The Biomeme SARS-CoV-2 system can help decentralize COVID-19 testing and offer rapid test results for patients in remote sites and low-resourced settings.

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