scholarly journals Characterization of the Spatial Variation of Microbial Communities in a Decentralized Subtropical Wastewater Treatment Plant Using Passive Methods

Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1157
Author(s):  
Marycarmen Verduzco Garibay ◽  
Alberto Fernández del Castillo ◽  
Osiris Díaz Torres ◽  
José de Anda ◽  
Carlos Yebra-Montes ◽  
...  
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Spatial Variation ◽  
Wastewater Treatment Plant ◽  
Bacterial Communities ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Microbial Interactions ◽  
Nitrifying Bacteria ◽  
Degrading Bacteria

Septic tanks (STs), up-flow anaerobic filters (UAFs), and horizontal-flow constructed wetlands (HFCWs) are cost-effective wastewater treatment technologies especially efficient in tropical and sub-tropical regions. In this study, the bacterial communities within a decentralized wastewater treatment plant (WWTP) comprising a ST, a UAF, and a HFCW were analyzed using high-throughput sequencing of the V3–V4 region of the 16S rRNA gene. Bacterial diversity and its spatial variation were analyzed at the phylum and family level, and principal component analysis (PCA) was applied to nitrogen- and organic-matter-degrading families. The highest percentage of nitrogen removal was seen in the HFCW (28% of total Kjeldahl nitrogen, TKN, and 31% of NH3-N), and our results suggest that families such as Rhodocyclaceae (denitrifying bacteria), Nitrospiraceae (nitrifying bacteria), and Rhodospirillaceae (sulfur-oxidizing bacteria) contribute to such removal. The highest percentage of organic matter removal was seen in the UAF unit (40% of biological oxygen demand, BOD5, and 37% of chemical oxygen demand, COD), where organic-matter-degrading bacteria such as the Ruminococcaceae, Clostridiaceae, Lachnospiraceae, and Syntrophaceae families were identified. Redundancy analysis demonstrated that bacterial communities in the HFCW were more tolerant to physicochemical changes, while those in the ST and the UAF were highly influenced by dissolved oxygen and temperature. Also, pollutant removal pathways carried out by specific bacterial families and microbial interactions were elucidated. This study provides a detailed description of the bacterial communities present in a decentralized WWTP located in a subtropical region.

scholarly journals Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant

2018 ◽  
Vol 78 (7) ◽  
pp. 1517-1524 ◽  
Author(s):  
Riqiang Li ◽  
Jianxing Wang ◽  
Hongjiao Li
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Removal Rate ◽  
Treatment Plant ◽  
Coking Wastewater ◽  
Naphthalene Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Abstract As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.

Development of Bacterial Consortia and Biodegradation Ability under Different PAH Stresses

2014 ◽  
Vol 1073-1076 ◽  
pp. 176-182
Author(s):  
Jing Ma ◽  
Shao Liang Zhang ◽  
Jun Feng Qu ◽  
Ai Hua Yan ◽  
Fu Chen
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Wastewater Treatment Plant ◽  
Bacterial Communities ◽  
Treatment Plant ◽  
Species Structure ◽  
Active Sludge ◽  
Environmental Pressures ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The aims of the present work were simulating actual environmental pollution to select and compare the bacterial communities under different environmental stresses such as phenanthrene and pyrene. Two bacterial consortia named as Phe consortium and Pyr consortium were enriched from activated sludge obtained from a wastewater treatment plant. The PCR-DGGE analysis showed that the original active sludge harbored abundantly diverse PAH-degrading bacteria at first, then under different environmental pressures the consortium species tended to be concentrated and had species structure differences. In addition, the abilities of the two bacterial consortia to remediate a mixture of 4 PAHs (50 mg Kg-1 each) in soil were studied. Of the 4 PAHs, greater than 80% of the fluorene and phenanthrene in soil were removed by the Phe consortium in just 7-days respectively; whereas the Pyr-consortium could degrade over 60% of anthracene and pyrene.

Savings with upgraded performance through improved activated sludge denitrification in the combined activated sludge–biofilter system of the Southpest Wastewater Treatment Plant

2008 ◽  
Vol 57 (8) ◽  
pp. 1287-1293 ◽  
Author(s):  
A. Jobbágy ◽  
G. M. Tardy ◽  
Gy. Palkó ◽  
A. Benáková ◽  
O. Krhutková ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Biological Treatment ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Process Efficiency ◽  
Initial Value ◽  
Initial Profile ◽  
Biological Treatment System

The purpose of the experiments was to increase the rate of activated sludge denitrification in the combined biological treatment system of the Southpest Wastewater Treatment Plant in order to gain savings in cost and energy and improve process efficiency. Initial profile measurements revealed excess denitrification capacity of the preclarified wastewater. As a consequence, flow of nitrification filter effluent recirculated to the anoxic activated sludge basins was increased from 23,000 m3 d−1 to 42,288 m3 d−1 at an average preclarified influent flow of 64,843 m3 d−1, Both simulation studies and microbiological investigations suggested that activated sludge nitrification, achieved despite the low SRT (2–3 days), was initiated by the backseeding from the nitrification filters and facilitated by the decreased oxygen demand of the influent organics used for denitrification. With the improved activated sludge denitrification, methanol demand could be decreased to about half of the initial value. With the increased efficiency of the activated sludge pre-denitrification, plant effluent COD levels decreased from 40–70 mg l−1 to < 30–45 mg l−1 due to the decreased likelihood of methanol overdosing in the denitrification filter

Evaluating the treatment performance of a full scale Activated Sludge Plant in Islamabad, Pakistan

2012 ◽  
Vol 7 (1) ◽  
Author(s):  
S. S. Fatima ◽  
S. Jamal Khan
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Suspended Solids ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Full Scale ◽  
Operational Parameter ◽  
Mixed Liquor ◽  
Treatment Performance

In this study, the performance of wastewater treatment plant located at sector I-9 Islamabad, Pakistan, was evaluated. This full scale domestic wastewater treatment plant is based on conventional activated sludge process. The parameters which were monitored regularly included total suspended solids (TSS), mixed liquor suspended solids (MLSS), mixed liquor volatile suspended solids (MLVSS), biological oxygen demand (BOD), and chemical oxygen demand (COD). It was found that the biological degradation efficiency of the plant was below the desired levels in terms of COD and BOD. Also the plant operators were not maintaining consistent sludge retention time (SRT). Abrupt discharge of MLSS through the Surplus Activated sludge (SAS) pump was the main reason for the low MLSS in the aeration tank and consequently low treatment performance. In this study the SRT was optimized based on desired MLSS concentration between 3,000–3,500 mg/L and required performance in terms of BOD, COD and TSS. This study revealed that SRT is a very important operational parameter and its knowledge and correct implementation by the plant operators should be mandatory.

Metro-environmental data approach for the prediction of chemical oxygen demand in new Nicosia wastewater treatment plant

2021 ◽  
Vol 221 ◽  
pp. 31-40
Author(s):  
A.S. Mubarak ◽  
Parvaneh Esmaili ◽  
Z.S. Ameen ◽  
R.A. Abdulkadir ◽  
M.S. Gaya ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Chemical Oxygen Demand ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Environmental Data

scholarly journals Investigation of the Efficiency of Phosphorus Removal Using Anionic Resins From the Continuous Flow in the Waste Stabilization Pond Outlet: A Case Study of Kaboodrahang Wastewater Treatment Plant

2019 ◽  
Vol 6 (1) ◽  
pp. 16-20
Author(s):  
Ali Akbar Rahmani Sarmazdeh ◽  
Mostafa Leili
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Phosphorus Removal ◽  
Continuous Flow ◽  
Removal Rate ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Cold Season ◽  
Stabilization Pond ◽  
The Mean

This research mainly aimed to investigate phosphorus removal from stabilization pond effluent by using anionic resins in the continuous flow mode of operation due to high amounts of phosphorus in the wastewater treatment plant effluent of Kaboodrahang, western Iran, as well as the violation from a prescribed effluent standard to discharge receiving the surface waters. For this purpose, the pilot was made of a plexiglass cylinder and other equipment such as pump and other accessories, as well as Purolite A-100 resin. The reactor effects on the desired study parameters were assessed in two warm and cold seasons. The results showed that the phosphorus concentrations reduced from 7-10 mg/L to 4-7 mg/L and the rate of phosphorus removal was higher in the hot season compared to the cold season. Moreover, the optimum temperature and pH were obtained 21ºC and 8.5, respectively. The mean inlet biological oxygen demand (BOD) was 150 mg/L for both warm and cold seasons, where the highest removal rate of 17% was obtained in the cold season. The mean chemical oxygen demand concentration of the pilot was 250 mg/L for both seasons, and the highest removal rate was observed in the cold season with an efficiency of 18%. Regarding the total suspended solids with the mean inlet of 230 mg/L, the highest removal efficiency was obtained 6% in the warm season.

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